• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.133-133
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• 1998

Tylopeptins A and B are novel peptides from methanol extract of fruit body of mushroom, Tylopilus neofelleus, These novel peptides were isolated by reverse-phase HPLC. And they have been shown to consist of one acetylated N -terminal residue, fourteen amino acids, and leucinol, C-terminal amino alcohol. Sequencial determination and complete 1H and 13C resonance assignments were based on positive ion F AB mass spectroscopy and two dimensional NMR techniques using HOHAHA, ROSEY, HMQC, and HMBC. These compounds are active against some Gram-positive bacteria, but inactive against phathogenic fungi and Gram-negative bacteria.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.2
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• pp.77-80
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• 2007

Macroalgal tissues can be used as indicating materials for environmental assessment using several algal biotechnology techniques. As bioassay test organisms, macroalgal tissues are required as an axenic state for suitable biological indicators. Callus formation and blade regeneration under suitable culture conditions are also useful for the tests. Quantitative method using tetrazolium chloride or $alamarBlue^{TM}$ is devised on a rapid assessment of the seaweed viability. The use of RT-PCR especially differential display technique should provide the means for the detection and isolation of the responding genes induced by the environmental stress. Seaweed thriving in more environmental changes might contain more diverse biologically active substances.

• Archives of Pharmacal Research
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• v.3 no.1
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• pp.31-36
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• 1980

Seeds or beans of 55 plants belonging to 31 families were screened by using several different types of red blood cells to find new lectins. In this paper, white kidney been (phaseolus vulgaris C.) was chosen to study biochemical properties of hemagglutinating proteins(lectins). An anion exchanger, DEAE Sephadex A-50, and polyacrylamide disc gel electrophoresis were main techniques used. From three main fractions eluted by stepwise NaCl gradient in 25mM Tris-HCI buffer on DEAE Sephadex column, principal lectin was identified.

• Molecules and Cells
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• v.24 no.2
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• pp.268-275
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• 2007

Protein phosphorylation plays a key role in signal transduction in cells. Since phosphoproteins are present in low abundance, enrichment methods are required for their purification and analysis. Chemical derivatization strategies have been devised for enriching phosphoproteins and phosphopeptides. In this report, we employed a strategy that replaces the phosphate moieties on serine and threonine residues with a biotin-containing tag via a series of chemical reactions. Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO)-depleted protein extracts prepared from Arabidopsis seedlings were chemically modified for 'biotin-tagging'. The biotinylated (previously phosphorylated) proteins were then selectively isolated by avidin-biotin affinity chromatography, followed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This led to the identification of 31 protein spots, representing 18 different proteins, which are implicated in a variety of cellular processes. Despite its current technical limitations, with further improvements in tools and techniques this strategy may be developed into a useful approach.

• Archives of Pharmacal Research
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• v.19 no.5
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• pp.406-410
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• 1996

The flowers of Chrysanthemum boreale afforded handelin, a unique guaianolide dimer and a mixture of n-hydrocarbons and n-hydrocarbon alcohols in addition to b-sitosterol and b-sitosterol glucoside. Detailed analysis of the $^{1}H$- and $^{13}C$-NMR spectra of handelin was carried out by the application of two-dimensional $^{1}H-^{1}H$-COSY and $^{1}H$- $^{13}C$ multiple-bond, multiple-quantum spectroscopic correlation techniques. Handelin was inactive in the in vitro anti-tumor activity.

• International Journal of Aeronautical and Space Sciences
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• v.18 no.3
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• pp.535-544
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• 2017

In-flight aircraft engine performance estimation is one of the key techniques for advanced intelligent engine control and in-flight fault detection, isolation and accommodation. This paper detailed the current performance degradation estimation methods, and an improved hybrid Kalman filter via velocity-based LPV (VLPV) framework for these needs is proposed in this paper. Composed of a nonlinear on-board model (NOBM) and VLPV, the filter shows a hybrid architecture. The outputs of NOBM are used for the baseline of the VLPV Kalman filter, while the system performance degradation factors on-line estimated by the measured real system output deviations are fed back to the NOBM for its updating. In addition, the setting of the process and measurement noise covariance matrices' values are also discussed. By applying it to a commercial turbofan engine, simulation results show the efficiency.

• Natural Product Sciences
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• v.22 no.1
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• pp.35-40
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• 2016

The first study on chemical constituents and biological activities of Clausena lansium (Lour.) Skeels (Rutaceae) growing in Vietnam has been done. Phytochemical investigation of n-hexane extract led to the isolation of five compounds: dihydroindicolactone (1), 8-geranyloxy psoralen (2), imperatorin (3), heraclenol (4) and indicolactone (5), in which this is the first report on the presence of dihydroindicolactone (1). Their structures were elucidated based on LC/MS/NMR hyphenated techniques as well as comparison with those of literature data. The n-hexane extract and its subfractions, ethanol 95% extract and several isolated compounds were evaluated for antifungal activity.

• Natural Product Sciences
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• v.14 no.4
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• pp.227-232
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• 2008

Phytochemical further investigation of the chloroform extract of the aerial parts of Crucianella maritima L. (Rubiaceae) growing in Egypt resulted in the isolation of a new anthraquinone; 3-formyl-1-hydroxy-2-methoxy anthraquinone (3) along with the four known compounds isolated for the first time from the genus Crucianella; alizarin-1-methyl ether (2), 1,4-dihydroxy-2-methoxy anthraquinone (5), 1, 3, 6-trihydroxy-2-methoxy anthraquinone (7) and the flavonol kaempferol (8), beside four known compounds previously isolated from the same plant. The structures of the isolated compounds were established based on different spectroscopic data including UV, IR, EIMS, 1D and 2D-NMR. Moreover, the antioxidant and antimicrobial activities of the isolated compounds were evaluated.

• Natural Product Sciences
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• v.4 no.4
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• pp.257-262
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• 1998

The extract of the aerial parts of Fumaria bastardii Bor. afforded 12 alkaloids belonging to the skeletally six different groups of the isoquinoline alkaloids. In this publication, the isolation and identification of protopine (1), corydaldine (2), oxyhydrastinine (3), (-)-fumaritine (4), (+)-fumariline (5), (-)-O-methylfumarophycine (12), (+)-bicuculline (10), $(-)-{\beta}-hydrastine$ (7), (-)-corlumine (11), (+)-tetrahydropalmatine (8), (-)-stylopine (6), and (+)-juziphine (9) are described. Their structures have been determined by using extensive spectroscopic techniques. This is the first report of the occurrence of these alkaloids in Fumaria bastardii of Turkish origin.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.10
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• pp.700-706
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• 2015

In this study, stability designing electronics mounted on launch vehicle for shock load(low/high frequency band) could be derived. For the low-frequency shock loads, CCA(circuit card assembly) has secured the structural integrity over the best natural frequency techniques. For the high-frequency shock load, the structural integrity could be ensured with applying device such as the insulation pad. When the EAR is applied, insulation effect of part application is good more than whole application.