• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.922-923
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• 2008

This paper is proposed to a novel DC-DC buck-boost converter added electric isolation by using a soft switching method. To be achieved of high efficiency system, the proposed converter is constructed by using a partial resonant circuit. The control switches using in the converter are operated with soft switching for a partial resonant method. The controlling switches are operated without increasing their voltage and current stresses by the soft switching technology. The result is that the switching loss is very low and the converter efficiency is high. And the proposed converter is added in a electric isolation. When the power conversion system is required to electric isolation, the proposed converter is adopted with system development of high efficiency. The soft switching operation and the system efficiency of the proposed converter is verified by digital simulation and experimental results.

• Proceedings of the KIEE Conference
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• 2008.10c
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• pp.142-144
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• 2008

This paper is studied on the characteristic analyses of a high performance buck-boost converter added electric isolation by using a soft switching method. To be achieved of a high performance system, the proposed buck-boost converter is constructed by using a partial resonant circuit. The control switches using in the converter are operated with soft switching for a Partial resonant method. The controlling switches are operated without increasing their voltage and current stresses by the soft switching technology. The result is that the switching loss is very low and the converter efficiency is high. And the proposed converter is added in a electric isolation. When the power conversion system is required to electric isolation, the proposed converter is adopted with the system development of high efficiency. The soft switching operation and system efficiency of the proposed converter is verified by digital simulation and experimental results.

• Journal of the Earthquake Engineering Society of Korea
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• v.24 no.4
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• pp.169-178
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• 2020

In a previous paper, ambient vibration tests were conducted on a cable stayed bridge with resilient-friction base isolation systems (R-FBI) to extract the dynamic characteristics of the bridge and compare the results with a seismic analysis model. In this paper, a nonlinear seismic analysis model was established for analysis of the bridge to compare the difference in seismic responses between nonlinear time history analysis and multi-mode spectral analysis methods in the seismic design phase of cable supported bridges. Through these studies, it was confirmed that the seismic design procedures of the "Korean Highway Bridge Design Code (Limit State Design) for Cable Supported Bridges" is not suitable for cable supported bridges installed with R-FBI. Therefore, to reflect the actual dynamic characteristics of the R-FBI installed on cable-supported bridges, an improved seismic design procedure is proposed that applies the seismic analysis method differently depending on the seismic isolation effect of the R-FBI for each seismic performance level.

• Journal of Microbiology and Biotechnology
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• v.34 no.9
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• pp.1919-1925
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• 2024

Effective isolation and sensitive detection of Pseudomonas aeruginosa (P. aeruginosa) is crucial for the early diagnosis and prognosis of various diseases, such as urinary tract infections. However, efficient isolation and simultaneous detection of P. aeruginosa remains a huge challenge. Herein, we depict a novel fluorescence assay for sensitive, enzyme-free detection of P. aeruginosa by integrating DNAzyme cascade-induced DNA tweezers and magnetic nanoparticles (MNPs)-based separation. The capture probe@MNPs is capable of accurately identifying target bacteria and transporting the bacteria signal to nucleic acid signals. Based on the DNAzyme cascade-induced DNA tweezers, the nucleic acid signals are extensively amplified, endowing the method with a high sensitivity and a low detection limit of 1 cfu/mL. In addition, the method also exhibits a wide detection of six orders of magnitudes. The proposed method could be extended to other bacteria detection by simply changing the aptamer sequence. Taking the merit of the high sensitivity, greatly minimized detection time (less than 1.5 h), enzyme-free characteristics, and stability, the proposed method could be potentially applied to diagnosing and preventing diseases caused by pathogenic bacteria.

• Korean Journal of Veterinary Service
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• v.22 no.3
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• pp.221-237
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• 1999

To investigate the specificity of rapid slide agglutination test for pullorum-gallinarum diseases and to obtain a basic data for avian salmonellosis control, salmonella isolation was peformed for the layer chickens positively reacted in pullonlm-typhoid agglutination test. The biochemical, serological and antimicrobial properties of the isolates were examined. The results obtained through this study were summarized as follows; 1. Of 2,384 chickens tested by the agglutination test, 606 chickens (25.4%) were positive reactors. 154 of 606 reactors and 49 of the non-reacting chickens were investigated for salmonella isolation, resulting in isolation of 68 strains of salmonellae from 27 chickens. 2. By organs, the isolation frequency from liver, cecum, spleen, ovary and gall bladder showed 8.9% (18 strains), 8.9% (18 strains), 7.4% (15 strains), 4.4% (9 strains) and 3.9% (8 strains), respectively. 3. By culture medium the combination of selenite broth and MacConkey agar revealed the highest isolation rate and the enrichment culture by delayed secondary enrichment culture method was found the most effective for salmonella isolation. 4. The serotypes of 68 salmonella isolates were identified as 3 strains of S pullorum, 24 strains of S gallinarum, 15 strains of S typhimurium, 8 strains of S enteritidis, 7 strains of S paratyphi A, 5 strains of S typhimurium and 6 strains of the other salmonellae. 5. The serotypes of 8 salmonella strains isolated from 49 chickens non-reacting in pullorum-typhoid agglutination test were identified as 3 strains of S typhimurium and 5 strains of S infantis. 6. When 24 chickens of which 68 strains of salmonellae isolated were examined by microplate agglutination test, the average antibody titer for pullorum antigen was $2^{5.25}$. The chickens at antibody titer between $2^3$ and $2^5$ showed the higher frequency of isolation as compared with the chickens at the other titers. 7. When salmonella isolates were tested the antimicrobial drug sensitivity by disk diffusion method, S paratyphi A were highly sensitive by 100% to ATM and GM, S typhimurium, by 88% to AM, CIP, IMP and TN, S infantis, by 100% to AM, CRO, ENR and PIP, S enteritidis,by 100% to IMP and PIP, S pullorum, by 100% to ATM, CRO, ENR and PIP and S gallinarum, by 92% to CRO, CIP and PIP.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.10a
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• pp.448-454
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• 2000

Piled multi-block system has been frequently adopted in the historic structures or monuments of cultural heritage. It is well known that such a structural system is very vulnerable to the earthquake shaking. If the structure is of slender type, then it may experience overturning at very low level intensity of ground shaking. One of the methods used to protect such structures from earthquake is seismic isolation system. But the behavior of multi-block systems mounted on the isolated basis is not well understood yet. In this paper we investigate the dynamic behavior of single slender rigid block mounted on the three different isolation systems, i.e., P-F system, FPS and LRB system. Sliding at the isolation interface of P-F system and FPS is formulated based on Coulomb friction. The mounted single block is assumed undergoing rocking or sticking only. Impacting of a single block is described using distinct element method (DEM). Free vibrations due to a prescribed initial conditions are studied. Responses to the harmonic excitation and earthquake motions are calculated

• Journal of the Korean Society for Precision Engineering
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• v.21 no.10
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• pp.137-146
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• 2004

This is the second of two companion papers concerned with the analysis and design of a pneumatic vibration isolation system. The properties of the system are clarified by observation of the transmissibility surface calculated by the models and algorithm developed in the first paper of this research. It Is shown that the nonlinear model proposed in this research is more closer to experimental results than the linear model that have been used in previous studies. The design optimization of the major design variables that affect the performance of the system is achieved by using the condition for attenuation, disturbance rejection and maximum damping in resonance peak. The design space search method is adopted for the optimization of the orifice area. The models, transmissibility calculation algorithms and design optimization techniques developed in this research are shown to be greatly helpful to the optimal design of the pneumatic vibration isolation system by experiment.

• Earthquakes and Structures
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• v.4 no.5
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• pp.527-555
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• 2013

This paper proposes probabilistic models for estimating the seismic demands on reinforced concrete (RC) bridges with base isolation. The models consider the shear and deformation demands on the bridge columns and the deformation demand on the isolation devices. An experimental design is used to generate a population of bridges based on the AASHTO LRFD Bridge Design Specifications (AASHTO 2007) and the Caltrans' Seismic Design Criteria (Caltrans 1999). Ground motion records are used for time history analysis of each bridge to develop probabilistic models that are practical and are able to account for the uncertainties and biases in the current, common deterministic model. As application of the developed probabilistic models, a simple method is provided to determine the fragility of bridges. This work facilitates the reliability-based design for this type of bridges and contributes to the transition from limit state design to performance-based design.

• The Transactions of the Korean Institute of Power Electronics
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• v.19 no.6
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• pp.522-529
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• 2014

This study proposes an isolation amplifier circuit for the sensing and feedback of inverter DC-link voltage, which is inevitable for the precise control of inverter output voltage. The isolation amplifier consists of a pulse-width modulator and a pulse transformer with dual secondary windings. The accuracy of the proposed circuit depends on the precise matching of filter parameters in dual secondary circuits. The influences of parameter inaccuracy on the amplifier performances are analyzed. A modified circuit is proposed to reduce the dependency on filter parameters. The validity of the proposed method is verified through simulation and experiment.

• Transactions on Electrical and Electronic Materials
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• v.3 no.4
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• pp.5-9
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• 2002

Chemical mechanical polishing (CMP) has become the preferred planarization method for multilevel interconnect technology due to its ability to achieve a high degree of feature level planarity. Especially, to achieve the higher density and greater performance, shallow trench isolation (STI)-CMP process has been attracted attention for multilevel interconnection as an essential isolation technology. Also, it was possible to apply the direct STI-CMP process without reverse moat etch step using high selectivity slurry (HSS). In this work, we determined the process margin with optimized process conditions to apply HSS STI-CMP process. Then, we evaluated the reliability and reproducibility of STI-CMP process through the optimal process conditions. The wafer-to-wafer thickness variation and day-by-day reproducibility of STI-CMP process after repeatable tests were investigated. Our experimental results show, quite acceptable and reproducible CMP results with a wafer-to-wafer thickness variation within 400$\AA$.