• 식물병연구
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• 제21권3호
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• pp.227-230
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• 2015

The ascomycete fungus Fusarium fujikuroi causes bakanae disease in rice and this disease has been reemerging in Korea. Other fungal species including F. graminearum and Magnaporthe oryzae are often associated with F. fujikuroi, hampering pure isolation of F. fujikuroi from rice. In this study, we modified a selective medium for F. fujikuroi as supplementing both pentachloronitrobenzene and hydrogen peroxide into minimal medium. This medium efficiently suppressed the vegetative growth of F. graminearum and M. oryzae, but did not significantly reduce F. fujikuroi growth, providing an efficient tool for isolating F. fujikuroi.

• 미생물학회지
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• 제23권1호
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• pp.43-48
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• 1985

철저한 혐기적 방법으로 배양분리한 결과 편성 혐기성세균의 분리율이 높아졌고, 검체당 평균 3.3주가 분리되었다. 혈액배지인 NATB배지와 BA배지에서 비혈액배지인 NAT배지와 GAM배지에서 보다 세균 분리효과가 우수했다. NATB배지에는 혐기성 그람 양성 구균에 좋은 분리효과를 나타내었고, 그람 음성 간균은 15주 분리되었다. 이 중 Bacteroides가 12주로서 가장 많이 존재하는 균종이었다.

• The Plant Pathology Journal
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• 제29권4호
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• pp.446-450
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• 2013

The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.

• 한국구조물진단유지관리공학회 논문집
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• 제14권6호
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• pp.93-99
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• 2010

본 논문에서는 상부구조에 있어서 보-기둥 강성비 변화에 따른 골조의 특성과 상부구조와 면진층 간의 진동주기비에 따른 면진효과를 분석해 보고, 상부골조의 주기와 목표 면진주기의 설정에 따라 면진효과가 어떻게 달라지는가에 대한 정보를 제공하여 향후 면진건물을 설계하기 위한 기본계획을 세우는데 있어서 필요한 기초 자료를 제공하고자 한다. 그 결과 건물골조의 경우 유효한 면진효과를 얻기 위해서는 최소한 상부구조의 고유진동주기 대비 2.5배 이상의 면진주기를 확보하고, 목표 면진주기를 2.0초 이상으로 설정하여 설계할 것을 추천한다.

• The Plant Pathology Journal
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• 제34권3호
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• pp.157-162
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• 2018

The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.

• 한국미생물·생명공학회지
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• 제22권2호
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• pp.210-216
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• 1994

Phage utilizing medium and BL agar supplemented with antibiotic Tc(tetracycline) were developed as selective media for the isolation and counting of bifidobacteria in dairy products. The former was based on the host specificity of phage. When bifidobacteria and Lactobacillus casei HY 2782 were mixed together in dairy product, L. casei HY 2782 was laysed by J1 phage which has host specificity to L. casei HY 2782 whereas bifidobacteria grew well on the selective medium added wIth J1 phage. The latter was found to inhibit the growth of S. salivarius subsp. thermophilus, L. acidophilus, and L. casei, but commercial bifidobacteria grew well in Tc-containing BL agar.

• 미생물학회지
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• 제21권4호
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• pp.213-220
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• 1983

Conditions for isolation of protoplasts from conidiospores of Trichoderma koningii ATCC 26113 were tested. Maximum production of conidial protoplasts was obtained by preincubation of conidiospores on liquid minimal medium for 8 1/2 hrs. and by reaction with cell wall lytic enzyme for 3 hrs. Among effective cell wall lytic enzymes (Driselase, p-Glucuronidase, Novozyme and Zymolyase), Driselase was the most effective one on the production of conidial protoplasts. The production of conidial protoplasts was also enhanced by addition of 2-Deoxy-D-Glucose $(25{\mu}g/ml)$ into liquid minimal medium. Over 70% of the initial swollen conidia, preincubated in liquid minimal medium supplemented with 2-Deoxy-D-Glucose $(25{\mu}g/ml)$, were converted to protoplasts by incubation with 2% (w/v) commercial lytic enzyme Driselase at $28^{\circ}C$ for 3 hrs. The reversion frequency of the conidial protoplasts was about 30 times (25-50%) higher than that of mycelial protoplasts (0.6-1.3%).

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2000년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2000
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• pp.424-431
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• 2000

After the Kobe earthquake(1995) in Japan, the Izmit earthquake(1997) in Turkey and the Chi-chi earthquake(1999) in Taiwan, the small-to-medium-sized earthquakes occurred in the Koreans peninsula and this shows the fact that Korea is not located in the safety zone of earthquake. The main concept of base isolation system is to reduce the member forces by decreasing the earthquake forces transmitted to superstructure instead of the conventional techniques of strengthening the structural members. This study investigates the effect of seismic response attenuation of computer floors using base isolation systems

• Journal of Plant Biology
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• 제29권1호
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• pp.11-18
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• 1986

Protopasts were isolated from cultured cells of potato (Solanum tuberosum L.) tuber tissue. The ability of callus formation from the culture cells was higher in cultivars Dejima and Superior than in Shimabara and Irish Cobbler on Lam's medium. Therefore, the former was used as sources for protoplast isolation. Friable calli were transferred to liquid media and cells in exponential phase were used for protoplast isolation. In both of Dejima and Superior, the yield of protoplasts was high in the enzyme solution of 2% Onozuka cellulase and 1% macerozyme. Also, viability of isolated protoplasts was very good. Thus, it seems that these protoplasts would be applicable to various aims of research.

• Computers and Concrete
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• 제8권6호
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• pp.693-715
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• 2011

The main object of this study is to evaluate the seismic response effects on a reinforced concrete building isolated by triple concave friction pendulum (TCFP) bearings. The site-response effects arise from the difference in the local soil conditions at the support points of the buildings. The local soil conditions are, therefore, considered as soft, medium and firm; separately. The results on the responses of the isolated building are compared with those of the non-isolated. The building model used in the time history analysis, which is a two-dimensional and eight-storey reinforced concrete building with and without the seismic isolation bearings and/or the local soil conditions, is composed of two-dimensional moment resisting frames for superstructure and of plane elements featuring plane-stress for substructure. The TCFP bearings for isolating the building are modelled as of a series arrangement of the three single concave friction pendulum (SCFP) bearings. In order to investigate the efficiency of both the seismic isolation bearings and the site-response effects on the buildings, the time history analyses are elaborately conducted. It is noted that the site-response effects are important for the isolated building constructed on soft, medium or firm type local foundation soil. The results of the analysis demonstrate that the site-response has significant effects on the response values of the structure-seismic isolation-foundation soil system.