Korean Journal of Microbiology (미생물학회지)
- Volume 21 Issue 4
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- Pages.213-220
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- 1983
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Isolation of protoplast from conidiospore of Trichoderma koningii
Trichoderma koningii의 conidiospore로부터의 원형질체 분리에 관하여
Abstract
Conditions for isolation of protoplasts from conidiospores of Trichoderma koningii ATCC 26113 were tested. Maximum production of conidial protoplasts was obtained by preincubation of conidiospores on liquid minimal medium for 8 1/2 hrs. and by reaction with cell wall lytic enzyme for 3 hrs. Among effective cell wall lytic enzymes (Driselase, p-Glucuronidase, Novozyme and Zymolyase), Driselase was the most effective one on the production of conidial protoplasts. The production of conidial protoplasts was also enhanced by addition of 2-Deoxy-D-Glucose
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