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http://dx.doi.org/10.5423/PPJ.OA.09.2017.0194

Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees  

Vasconcelos, Camila Vilela (Universidade Estadual de Goias)
Pereira, Fabiola Teodoro (Universidade Estadual de Goias)
Duarte, Elizabeth Amelia Alves (Universidade Federal do Reconcavo da Bahia)
de Oliveira, Thiago Alves Santos (Faculdade Maria Milza)
Peixoto, Nei (Universidade Estadual de Goias)
Carvalho, Daniel Diego Costa (Universidade Estadual de Goias)
Publication Information
The Plant Pathology Journal / v.34, no.3, 2018 , pp. 157-162 More about this Journal
Abstract
The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.
Keywords
algal spot disease; algal isolation; Mangifera indica;
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1 Sales-Campos, C. and Andrade, M. C. N. 2010. Most favorable temperature and culture medium for the mycelial growth of a strain of Lentinus strigosus from the Amazon region. Arq. Inst. Biol. 77:539-543.
2 Suto, Y. and Ohtani, S. 2009. Morphology and taxonomy of fve Cephaleuros species (Trentepohliaceae, Chlorophyta) from Japan, including three new species. Phycologia 48:213-236.   DOI
3 Suto, Y. and Ohtani, S. 2011. Morphological features and chromosome numbers in cultures of five Cephaleuros species (Trentepohliaceae, Chlorophyta) from Japan. Phycol. Res. 59:42-51.   DOI
4 Suutari, M., Majaneva, M., Fewer, D. P., Voirin, B., Aiello, A., Friedl, T., Chiarello, A. G. and Blomster, J. 2010. Molecular evidence for a diverse green algal community growing in the hair of sloths and a specifc association with Trichophilus welckeri (Chlorophyta, Ulvophyceae). BMC Evol. Biol. 10:86-97.   DOI
5 Agrios, G. N. 2005. Plant pathology. 5th ed. Elsevier Academic Press, Amsterdam, The Netherlands.
6 Boedeker, C., Karsten, U., Leliaert, F. and Zuccarello, G. C. 2013. Molecular, biochemical and morphological data suggest an affliation of Spongiochrysis hawaiiensis with the Trentepohliales (Ulvophyceae, Chlorophyta). Phycol. Res. 61:133-144.   DOI
7 Chowdary, Y. B. K. 1969. Induction of reproductive organs in Cephaleuros virescens. Indian J. Microbiol. 3:153-158.
8 Ferreira, D. F. 2011. Sisvar: a computer statistical analysis system. Cienc. Agrotec. 35:1039-1042.   DOI
9 Hamby, R. K., Sims, L., Issel, L. and Zimmer, E. 1988. Direct ribosomal RNA sequencing: optimization of extraction and sequencing methods for work with higher plants. Plant Mol. Biol. Rep. 6:175-192.   DOI
10 Han, K., Park, M., Park, J. and Shin, H. 2011. First report of algal leaf spot associated with Cephaleuros virescens on greenhouse grown Ficus benghalensis in Korea. Australas Plant Dis. Notes 6:72-73.   DOI
11 Holcomb, G. E. 1986. Hosts of the parasitic alga Cephaleuros virescens in Louisiana and new host records for the continental United States. Plant. Dis. 70:1080-1083.   DOI
12 Jose, G. and Chowdary, Y. B. K. 1977. Karyological studies on Cephaleuros Kunze. Acta Bot. Indica 5:114-122.
13 Ponmurugan, P., Saravanan, D. and Ramya, M. 2010. Culture and biochemical analysis of a tea algal pathogen, Cephaleuros parasiticus. J. Phycol. 46:1017-1023.   DOI
14 Leliaert, F., Smith, D. R., Moreau, H., Herron, M. D., Verbruggen, H., Delwiche, C. F. and Clerck, O. 2012. Phylogeny and molecular evolution of the green algae. Crit. Rev. Plant Sci. 31:1-46.   DOI
15 Lopez-Bautista, J. M., Rindi, F. and Guiry, M. D. 2006. Molecular systematics of the subaerial green algal order Trentepohliales: an assessment based on morphological and molecular data. Int. J. Syst. Evol. Microbiol. 56:1709-1715.   DOI
16 Malagi, G., Santos, I., Mazaro, S. M. and Guginski, C. A. 2011. Deteccao de mancha-de-alga (Cephaleuros virescens Kunze) em citros no estado do Parana. Revista Brasileira de Agrociencia 17:148-152.
17 Nelson, S. C. 2008. Cephaleuros species, the plant-parasitic green algae. University of Hawaii, Honolulu, HI. 6 pp.
18 Ponmurugan, P., Baby, U. I. and Rajkumar, R. 2007. Growth, photosynthetic and biochemical responses of tea cultivars infected with various diseases. Photosynthetica 45:143-146.   DOI
19 Ponmurugan, P., Saravanan, D., Ramya, M., Srinivasan, T. R., Baby, U. U. and Ajay, D. 2009. Studies on Cephaleuros parasiticus Karst, a pathogenic alga causing red rust disease in tea plantations. J. Plant. Crops 37:70-73.
20 Ren, H., Liu, B., Ma, C., Zhao, L. and Ren, N. 2013. A new lipidrich microalga Scenedesmus sp. strain R-16 isolated using Nile red staining: effects of carbon and nitrogen sources and initial pH on the biomass and lipid production. Biotechnol. Biofuels 6:143.   DOI
21 Rindi, F., Lam, D. W. and Lopez-Bautista, J. M. 2009. Phylogenetic relationship and species circumcription in Trentepohlia and Printzina (Trentepohliales, Chlorophyta). Mol. Phylogenet. Evol. 52:329-339.   DOI