• Title/Summary/Keyword: isolation, identification

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Isolation and Identification of Dextranase Production Strains and Enzyme Production (Dextranase 생산균주의 분리, 동정 및 효소생산)

  • Lee, Jong-Tae;Yi, Dong-Heui;Kwak, Yi-Seong;Kim, Young-Ho;Sung, Hyun-Soon;Kim, Chan-Jo
    • Microbiology and Biotechnology Letters
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    • v.23 no.4
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    • pp.405-410
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    • 1995
  • In order to screen dextranase with high dextranolytic activity from microbial origins, dextranase producing fungal isolates were isolated from soil of the Taeion area. 197 strains with dextranolytic activities were isolated, out of which 3 strains with high dextranolytic activities were selected in the first screening. A strain (GR-98) with a best dextranolytic activity was selected in the second screening. The strain was identified to be similiar Aspergillus ustus by the morphological and cultural characteristics. The optimum culture temperature and initial pH for the dextranase production of the strain was 30$\circ$C and 7.0, respectively. The optimum culture medium was composed of 2% dextran, 0.3% KNO$_{3}$, 0.05% K$_{2}$HPO$_{4}$, 0.02% MgSO$_{4}$-7H$_{2}$O, 0.05% KC1, and 2.5 $\mu$g/ml pyridoxamine, and the enzyme production was maximum when the strain was subcultured at 30$\circ$C for 7 days.

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Optimizing and Identification of Design Parameters of a Cylindrical Hydraulic Engine Mount by an Optimization Method (최적화 기법에 의한 원통형 유체 엔진마운트의 설계변수 동정 및 최적화)

  • Ahn, Young-Kong
    • Journal of Power System Engineering
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    • v.21 no.3
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    • pp.66-73
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    • 2017
  • In order to identify the design parameters of a hydraulic engine mount with a nonlinear characteristics, an experimental method has been used generally. The method takes a considerable time and expense because of preparing an experimental apparatus, conducting a test, and analyzing results. Therefore, this paper presents a simple method to identify the design parameters of a cylindrical hydraulic engine mount, and optimize the design parameters. The physical model and mathematical equations of the mount were derived, and values of the design parameters of the mount were identified by optimization method with minimizing difference between the analytical results with the equations and the experimental results. This method is more simpler than the conventional experiment method and identify successfully the design parameters. In addition, the technique can optimize the design parameters of the mount to improves the isolation performance of the mount.

Isolation and Identification of Aldehyde Producing Methanol Utilizing Yeast (메탄올 자화성 효모의 분리, 동정 및 Aldehyde 생산)

  • 윤병대;김희식;권태종;양지원;권기석;이현선;안종석;민태익
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.630-636
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    • 1992
  • Hansenula nonfermentans KYP-l was selected and identified from 19 methanol utilizing yeasts isolated from soil samples by the enrichment culture technique. This strain showed a high cell concentration and a high aldehyde production. Aldehyde production was carried out in a resting cell system using methanol utilizing yeast as a biocatalyst. The molar yield of acetaldehyde was the highest among the aldehyde investigated, and the maximum amount of aldehyde was produced by cells obtained from a 40 hours' culture.

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Constituents from Hypericum ascyron (물레나물 (Hypericum ascyron)의 성분)

  • Chae, Sung-Wook;Lee, So-Young;Kim, Ju-Sun;Bae, Ki-Hwan;Kim, Sung-Kyu;Kang, Sam-Sik
    • Korean Journal of Pharmacognosy
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    • v.37 no.3
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    • pp.162-168
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    • 2006
  • The isolation and identification of a new xanthone (6-0-palmitolyl-1,6-dihydroxy-5,7-dimethoxyxanthone) along with n-alkanols, fatty acids, 1,6-dihydroxy-5,7-dimethoxyxanthone, 1,7-dihydroxykanthone, betulinic acid, ${\beta}-sitosterol$, methyl 4-hydroxybenzoate, quercetin, and kaempferol from the aerial parts of Hypericum ascyron was reported. The structures were elucidated by spectroscopic methods, mainly NMR and mass spectrometry.

Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees

  • Vasconcelos, Camila Vilela;Pereira, Fabiola Teodoro;Duarte, Elizabeth Amelia Alves;de Oliveira, Thiago Alves Santos;Peixoto, Nei;Carvalho, Daniel Diego Costa
    • The Plant Pathology Journal
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    • v.34 no.3
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    • pp.157-162
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    • 2018
  • The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.

Isolation and Identification of a Soil Actinomycetes YBE-316 Producing an Antitumor Antibiotic (항암성 항생물질을 생산하는 토양 방선균 YBE-316의 분리 및 동정)

  • Shin, Jin-E;Park, Jae-Hong;Bai, Dong-Hoon;Yu, Ju-Hyun
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.297-303
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    • 1995
  • Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

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Isolation and Identification of 13-Deacetyl-taxchinin I, a New Taxoid from Plant Cell Cultures of Taxus chinensis (식물세포 Taxus chinensis 배양으로부터 신물질 13-Deacetyl-taxchinin I의 분리 및 동정)

  • 김진현;기은숙;유시용;최형균;홍승서;이현수
    • KSBB Journal
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    • v.15 no.6
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    • pp.560-565
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    • 2000
  • 13-Deacetyl-taxchinin I, a taxoid having a rearranged 11(15\longrightarrow1)-abeo-taxane skeleton, has been isolated and identified from plant cell cultures of Taxus chinensis. The compound has not previously been encountered in nature. Its structure was elucidated by 1-and 2D NMR techniques including H-H COSY, HMQC, and HMBC experiments. This taxoid also provides information for better understanding of structure-activity relationships and biosynthesis, as well as improving the quality control of paclitaxel production.

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MODELING AND PARAMETER IDENTIFICATION FOR A PASSIVE HYDRAULIC MOUNT

  • Zhang, Y.X.;Zhang, J.W.;Shangguan, W.B.;Feng, Q.Sh.
    • International Journal of Automotive Technology
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    • v.8 no.2
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    • pp.233-241
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    • 2007
  • A lumped parameter model is proposed for the analysis of dynamic behaviour of a Passive Hydraulic Engine Mount (PHEM), incorporating inertia track and throttle, which is characterized by effective and efficient vibration isolation behaviour in the range of both low and high frequencies. Most of the model parameters, including volume compliance of the throttle chamber, effective piston area, fluid inertia and resistance of inertia track and throttle are identified by an experimental approach. Numerical predictions are obtained through a finite element method for responses of dynamic stiffness of the rubber spring. The experiments are made for the purpose of PHEM validation. Comparison of numerical results with experimental observations has shown that the present PHEM achieves good performance for vibration isolation.