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Reverse-Phase HPLC Method for Identification of Diastereomeric Constituents from Sasa borealis  

Jeong Yeon Hee (College of Pharmacy, Ewha Womans University)
Lee Jun (College of Pharmacy, Ewha Womans University)
Kwon Youngjoo (College of Pharmacy, Ewha Womans University)
Seo Eun-Hyoung (College of Pharmacy, Ewha Womans University)
Publication Information
YAKHAK HOEJI / v.50, no.1, 2006 , pp. 21-25 More about this Journal
Abstract
Reiterated normal-phase column chromatography lead to the isolation and purification of six known compounds but for the first time from the whole plant of Sasa borealis (Hack.) Makino (Gramineae): tricin 4'-O-(erythro-${\beta}$-guaia-cylglyceryl) ether (1), tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether (2), tricin 4'-O-[erythro-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (3), tricin 4'-O-[threo-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (4), (-)-pinoresinol (5), and vanillin (6). The structures of the compounds (1-6) were established based on interpretation of high resolution NMR (COSY, HSQC, HMBC, and NOESY) spectral data. In particular, compounds 1 and 3 were diastereomers of compounds 2 and 4, respectively. These two sets of diastereomers were able to be simultaneously identified and quantified by a gradient reversed-phase HPLC method with UV photodiode array, This sensitive HPLC method is noteworthy as a simultaneous separation and identification method to test the extract of the family Gramineae which contains these compounds.
Keywords
Sasa borealis; Gramineae; simultaneous isolation and identification by HPLC;
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