• Title/Summary/Keyword: isolation, identification

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Construction of Probability Identification Matrix and Selective Medium for Acidophilic Actinomycetes Using Numerical Classification Data

  • Seong, Chi-Nam;Park, Seok-Kyu;Michael Goodfellow;Kim, Seung-Bum;Hah, Yung-Chil
    • Journal of Microbiology
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    • v.33 no.2
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    • pp.95-102
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    • 1995
  • A probability identification matrix of acidophilic Streptomyces was constructed. The phenetic data of the strains were derived from numerical classification described by Seong et al. The minimum number of diagnostic characters was determined using computer programs for calculation of different separation indices. The resulting matrix consisted of 25 clusters versus 53 characters. Theoretical evaluation of this matrix was achieved by estimating the chuster overlap and the identification scores for the Hypothetical Median Organisms (HMO) and for the representatives of each cluster. Cluster overlap was found to be relatively small. Identification scores for the HMO and the randomly selected representatives of each cluster were satisfactory. The matrix was assessed practically by applying the matrix to the identification of unknown isolates. Of the unknown isolates, 71.9% were clearly identified to one of eight clusters. The numerical classification data was also used to design a selective isolation medium for antibiotic-producing organisms. Four chemical substances including 2 antibiotics were determined by the DLACHAR program as diagnostic for the isolation of target organisms which have antimicrobial activity against Micrococcus luteus. It was possible to detect the increased rate of selective isolation on the synthesized medium. Theresults show that the numerical phenetic data can be applied to a variety of purposes, such as construction of identification matrix and selective isolation medium for acidophilic antinomycetes.

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Rapid identification of Burkholderia glumae from diseased seeds

  • Noh, Tae-Hwan;Song, Wan-Yeob;Kang, Mi-Hyung;Hyung Moo kim;Lee, Du-Ku;Park, Jong-Cheol;Shim, Hyeong-Kwon
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.136.1-136
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    • 2003
  • Bacterial grain rot by Burkholderia gluae cause severe damage in seedling and grain of rice after heading season. This seed-borne pathogen play a role as first infection agent that could be cause disease following cropping season. Until now the direct isolation of the bacteria has some trouble by interference of other bacteria existed inside seed. This study established convenient identification method as simple isolation with KB medium from seed showing symptom and using PCR identification. By this isolation method, B. glumae was isolated from 40 to 50% in brown rice and inner hull, however, there were saprophytic bacteria and fungi outer hull. In PCR identification with Ogf4 and Ogr3 primer to these 25 isolates, the amplified products were presented in all of the collections but not in 10 saprophytic germs. The isolation rate was constant to 3 months stored seeds. This result provide a rapid and convenient isolation and identification of B. glumae.

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Time and frequency domain identification of seismically isolated structures: advantages and limitations

  • Kampas, G.;Makris, N.
    • Earthquakes and Structures
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    • v.3 no.3_4
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    • pp.249-270
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    • 2012
  • This paper investigates the effectiveness of widely used identification methods to identify the response of seismically isolated structures supported on bearings with bilinear behavior. The paper shows that while both time domain and frequency domain methods predict with high accuracy the modal characteristics of structures isolated by linear isolation system, their performance degrades appreciably when the isolation system exhibits bilinear behavior even when its strength assumes moderate values (say 5% of the weight). The paper also shows that the natural period of isolated structure that results from bilinear isolation systems can be satisfactorily predicted with wavelet analysis.

A Method to Improve Isolation for Passive RFID Applications (수동형 RFID 시스템 적용을 위한 Isolation 개선 방법)

  • Kim, Jae-Kwon;Burm, Jin-Wook
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.44 no.4 s.316
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    • pp.45-49
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    • 2007
  • The isolation between transmitter and receiver has been a critical problem in a pulse-modulated RFID (Radio Frequency Identification) system where transmitter and receiver use the identical frequency. A method using a switch, a mixer, a BPF (band-pass filter), and an additional oscillator in the transmitter is proposed to improve the isolation between transmitter and receiver in an RFID system. The Proposed system up-converts the outgoing wave from a frequency different from the received signal and improves the isolation. The proposed method provided additional 20 dB improvement on the isolation.

Design and Modeling of a 3-DOF Precision Stage for Vibration Isolation (제진을 위한 3 자유도 정밀 스테이지의 설계와 모델링)

  • Moon, Jun-Hee;Kim, Hwa-Soo;Pahk, Heui-Jae
    • Journal of the Korean Society for Precision Engineering
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    • v.24 no.3 s.192
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    • pp.124-133
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    • 2007
  • Active vibration isolation systems need the following performance specifications which are different from those of existing positioning systems: usage of seismic sensors, strict suppression of phase lead/lag in signal processing for sensors and actuators, excellent control in low frequency range and so on. In consideration of such specifications, a 3-DOF precision stage for vibration isolation is designed and modeled based on the physical characteristics. Then the major parameters such as spring constants and damping coefficients are valued by the system identification method using empirical transfer function. Finite element analysis is used as a verification and simulation tool throughout this research. This paper lays the foundation for the future research on the control of the active vibration isolation system.

Isolation and Identification of Red Color Pigments from the Korean Lithospermum erythrorhizon (한국산 자초 적색소의 분리 및 확인)

  • 이제헌;오문헌;이희봉
    • The Korean Journal of Food And Nutrition
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    • v.13 no.4
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    • pp.379-382
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    • 2000
  • Conditions of isolation for the red color pigments from the Korean Lithospermum erythrorhizon were investigated and identification of the red pigment was analysed. Non-polar solvents were more effective than water. Especially, 95% ethanol was observed as optimum solvent for the pigments extraction. About 20 minutes at 40$^{\circ}C$ with 95% ethanol was enough for the extraction of the red pigments. The major pigment was analysed as acetylshikonin by TLC, IR, NMR and GC/MS.

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Satellite Fault Detection and Isolation Scheme with Modified Adaptive Fading EKF

  • Lim, Jun Kyu;Park, Chan Gook
    • Journal of Electrical Engineering and Technology
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    • v.9 no.4
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    • pp.1401-1410
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    • 2014
  • This paper presents a modified adaptive fading EKF (AFEKF) for sensor fault detection and isolation in the satellite. Also, the fault detection and isolation (FDI) scheme is developed in three phases. In the first phase, the AFEKF is modified to increase sensor fault detection performance. The sensor fault detection and sensor selection method are proposed. In the second phase, the IMM filer with scalar penalty is designed to detect wherever actuator faults occur. In the third phase of the FDI scheme, the sub-IMM filter is designed to identify the fault type which is either the total or partial fault. An important feature of the proposed FDI scheme can decrease the number of filters for detecting sensor fault. Also, the proposed scheme can classify fault detection and isolation as well as fault type identification.

Isolation and Identification of Pathogenic Microorganisms from Soybean Sprouts

  • Kim, Hye-Jung;Koo, Kyoung-Mo;Kim, Gi-Nahm;Lee, Dong-Sun;Paik, Hyun-Dong
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.305-309
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    • 2002
  • Raw soybean sprouts were tested for contamination with the following bacteria which have potential for pathogenesis or food spoilage : Salmonella spp., Escherichia coli O157:H7, Yersinia enterocolitica, Vibrio parahae-molyticus, Aeromonas hydrophila, Plesidomonas shigeloides, Pseudomonas aeruginosa, Staphylococcus aureus, Lis-teria monocytogenes, Bacillus cereus, Clostridium perfringens, Campylobacter jejuni, Erwinia spp., and Fusarium spp. Three of the above strains were isolated from the sprouts, and identified by morphological and biochemical methods including an API kit and ATB automated identification system. The isolate cultured in Cereus selective agar, a selective medium, was a Gram-positive, rod shaped, anaerobic spore former. The biochemical and culture tests revealed the following characteristics: catalase-positive, no growth on Simmon's citrate, NO₂ production and requirement of arginine for growth; the ATB automated identification system gave 99.8 % agreement for the identification of Bacillus cereus to the species level. The isolate cultured in Macconkey agar selective medium was Gram-negative, rod shaped and a gas former; the ATB-system gave 99.9% agreement for the identification of Aeromonas hydrophila to the species level. The isolate found in Pseudomonas isolation agar was Gram-negative, rod shaped, cytochrome oxidase-positive, a reducer of nitrates to nitrogen, and pyocyanin producer; the ATB-system gave 99.9 % agreement for the identification of Pseudomonas aeruginosa to the species level. These results indicate that the three bacteria species present in the soybean sprouts were Bacillus cereus, Aero-monas hydrophila, and Pseudomonas aeruginosa. Salmonella spp., Escherichia coli O157:H7, and Yersinia enter-ocolitica, which are associated with serious disease in humans, were not isolated from soybean sprouts examined in this study.

Components of Potentilla Species -Isolation and Identification of 3, 3', 4-tri-O-methylellagic acid- (Potentilla속 식물의 성분연구 -3, 3', 4-tri-O-methylellagic acid의 분리 및 동정-)

  • Kim, Hack-Seang
    • YAKHAK HOEJI
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    • v.33 no.6
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    • pp.377-379
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    • 1989
  • The methylated compound of ellagic acid was isolated from Potentilla chinensis (Rosaceae). The isolated compound was 3, 3', 4-tri-O-methylellagic acid, [2, 3, 7-trimethoxy-8-hydroxy[1] benzopyrano-[5, 4, 3, cde][1] benzopyran-5, 10-dione], $C_{17}H_{12}O_8$, m.p. $293-295^{\circ}C$. The isolation of trimethylellagic acid was conducted by the column chromatography and the identification of the compound was carried out by the methods of IR, NMR and MS spectroscopy.

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