• 한국지진공학회논문집
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• 제14권2호
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• pp.1-13
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• 2010

본 논문에서는 비선형 지진격리교량의 최적 설계 방법을 제시하였다. 최적설계를 위한 목적함수로는 교각과 지진격리장치의 파괴확률을 고려하였으며, 상충하는 두 목적함수를 동시에 최적화하는 다수의 해를 효율적으로 검색하고자 유전자 알고리즘에 기반한 다목적 최적화기법을 도입하였다. 또한, 최적화 과정에서 요구되는 다수의 비선형 시간이력해석을 수행하지 않고도 교량의 확률적 응답을 효율적으로 예측할 수 있는 추계학적 선형화 방법을 접목하였다. 제시하는 방법의 효율성을 검증하기 위한 수치 예로서 실제 교량인 남한강교를 고려하였고, 제안하는 방법과 기존 비선형 시간이력해석을 이용한 생애주기비용 기반 설계법을 각각 적용하여 내진성능을 비교하였다. 내진성능을 비교한 결과, 제시하는 방법이 기존의 비용에 기반한 최적설계보다 우수한 성능 및 경제성을 보임을 검증하였다. 또한, 다양한 지진하중에 대해서도 제안된 방법이 보다 개선된 성능을 보임을 확인하였다.

• 전자공학회논문지C
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• 제36C권6호
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• pp.44-51
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• 1999

HMM-Net은 은닉 마르코프 모델(HMM)의 계산과정을 신경망 구조로 구현한 것으로, HMM이 갖고 있는 시계열 모델링 능력과 신경망이 갖고 있는 우수한 변별력을 결합한 것이다. HMM-Net 분류기를 학습하는 방법으로는 HMM의 학습에 이용되는 最尤法(ML)과 신경망 학습의 最小二乘誤差法(MMSE)를 적용할 수 있다. 이들 중 ML이 MMSE보다 안정된 학습을 보장하는 반면 초기 학습조건을 적절하게 설정하였을 경우에는 MMSE가 ML보다 우수하다고 알려져 있다[3]. 따라서 이 논문에서는 먼저 ML을 이용하여 초기학습을 수행한 다음 보다 학습성능이 우수한 MMSE로 바꾸어 최적 또는 준최적으로 학습하는 하이브리드 학습법(ML/MMSE)을 제안한다. 실험용 시계열 패턴으로 /0/부터 /9/까지의 고립 숫자음을 이용하여 실험한 결과, 제안한 방법이 학습특성 및 인식률면에서 ML이나 MMSE만을 이용하는 기존의 방법보다 우수하였음을 확인하였다.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.357-363
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• 2011

Recently, we found that Arthrobacter crystallopoietes N-08 isolated from soil directly produces trehalose from maltose by a resting cell reaction. In this study, the optimal set of conditions and substrate specificity for the trehalose production using resting cells was investigated. Optimum temperature and pH of the resting cell reaction were $55^{\circ}C$ and pH 5.5, respectively, and the reaction was stable for two hours at $37{\sim}55^{\circ}C$ and for one hour at the wide pH ranges of 3~9. Various disaccharide substrates with different glycosidic linkages, such as maltose, isomaltose, cellobiose, nigerose, sophorose, and laminaribiose, were converted into trehalose-like spots in thin layer chromatography (TLC). These results indicated broad substrate specificity of this reaction and the possibility that cellobiose could be converted into other trehalose anomers such as ${\alpha},{\beta}$- and ${\beta},{\beta}$-trehalose. Therefore, the product after the resting cell reaction with cellobiose was purified by ${\beta}$-glucosidase treatment and Dowex-1 ($OH^-$) column chromatography and its structure was analyzed. Component sugar and methylation analyses indicated that this cellobiose-conversion product was composed of only non-reducing terminal glucopyranoside. MALDI-TOF and ESI-MS/MS analyses suggested that this oligosaccharide contained a non-reducing disaccharide unit with a 1,1-glucosidic linkage. When this disaccharide was analyzed by $^1H$-NMR and $^{13}C$-NMR, it gave the same signals with ${\alpha}$-D-glucopyranosyl-(1,1)-${\alpha}$-D-glucopyranoside. These results suggest that cellobiose can be converted to ${\alpha},{\alpha}$-trehalose by the resting cells of A. crystallopoietes N-08.

• Molecules and Cells
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• 제40권9호
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• pp.685-695
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• 2017

Obesity and the metabolic disorders that constitute metabolic syndrome are a primary cause of morbidity and mortality in the world. Nonetheless, the changes in the proteins and the underlying molecular pathways involved in the relevant pathogenesis are poorly understood. In this study a proteomic analysis of the visceral adipose tissue isolated from metabolically healthy and unhealthy obese patients was used to identify presence of altered pathway(s) leading to metabolic dysfunction. Samples were obtained from 18 obese patients undergoing bariatric surgery and were subdivided into two groups based on the presence or absence of comorbidities as defined by the International Diabetes Federation. Two dimensional difference in-gel electrophoresis coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was carried out. A total of 28 proteins were identified with a statistically significant difference in abundance and a 1.5-fold change (ANOVA, $p{\leq}0.05$) between the groups. 11 proteins showed increased abundance while 17 proteins were decreased in the metabolically unhealthy obese compared to the healthy obese. The differentially expressed proteins belonged broadly to three functional categories: (i) protein and lipid metabolism (ii) cytoskeleton and (iii) regulation of other metabolic processes. Network analysis by Ingenuity pathway analysis identified the $NF{\kappa}B$, IRK/MAPK and PKC as the nodes with the highest connections within the connectivity map. The top network pathway identified in our protein data set related to cellular movement, hematological system development and function, and immune cell trafficking. The VAT proteome between the two groups differed substantially between the groups which could potentially be the reason for metabolic dysfunction.

• 농업과학연구
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• 제40권4호
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• pp.367-375
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• 2013

The enzymatic interesterification was performed to produce structured lipids (SLs) with palm mid fraction (PMF) and stearic ethyl ester (STEE) for 1, 3, 6, 9, 12 and 15 hr at $80^{\circ}C$. The reaction was catalyzed by Lipozyme TLIM (immobilized lipase from Thermomyces lanuginosus, amount of 20% by weight of total substrates) in a shaking water bath set at 180 rpm. The optimum condition for synthesis of asymmetric SLs were: substrate molar ratio 1:0.5 (PMF:STEE, by weight), reaction time 6 hr, enzyme 20% (wt%, water activity=0.085) of total substrate and reaction temperature $80^{\circ}C$. After reaction at optimized condition, triacylglycerols (symmetrical and asymmetrical TAGs) from reactants were isolated. POP/PPO (1,3-palmitoyl-2-oleoyl glycerol or 1,2-palmitoyl-3-oleoyl glycerol), POS/PSO (palmitoyl-oleoyl-stearoyl glycerol or palmitoyl-stearoyl-oleoyl glycerol), SOS/SSO (1,3-stearoyl-2-oleoyl glycerol or 1,2-stearoyl-3-oleoyl glycerol) were obtained by solvent fractionation. Finally, refined SLs contained stearic acid of 16.91%. Solid fat index and thermogram of the refined SLs were obtained using differential scanning calorimetry. The degree of asymmetric triacylglycerol in the refined SLs was analyzed by Ag-HPLC equipped with evaporated light scattering detector (ELSD). The refined SLs consisted of symmetric TAG of 41.15 area% and asymmetric TAG of 58.85 area%.

• 한국식품과학회지
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• 제52권4호
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• pp.357-362
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• 2020

본 연구는 조직감 및 기능성 면에서 우수한 식물성 대체육의 제조를 위하여 부원료로 버섯을 첨가한 압출성형 대체육의 품질특성에 대하여 살펴보았다. 대체육의 원료는 분리대두단백 50%, 글루텐 40%, 옥수수 전분 10%를 섞은 것을 기본배합(base)으로 하였다. 버섯 첨가함량을 기본배합 대비 0, 4, 8, 12%로 한 총 네개의 시료를 수분함량 55%, 스크루 속도 150 rpm, 바렐 온도를 170℃로 하여 냉각 사출구를 통해 20℃로 사출하였다. 압출성형 후 물리적 특성으로 경도, 탄성, 응집성, 씹힘성과 절단 강도, 조직잔사지수, 보수력을 측정하였고, 화학적 특성으로 수용성 질소지수, 단백질 소화율과 DPPH 라디칼 소거활성을 측정하였다. 버섯 첨가함량이 증가함에 따라 경도, 탄성, 응집성, 씹힘성과 절단강도가 모두 증가하였으며, 조직잔사지수 역시 증가하였다. 반면에, 버섯 첨가함량이 증가할수록 보수력과 수용성 질소지수는 감소하였으며, 단백질 소화율도 다소 감소하였다. DPPH 라디칼 소거활성은 버섯 첨가함량의 증가에 따라 유의적인 증가를 나타내었다. 압출성형 대체육에 버섯의 첨가는 대체육의 조직감을 개선하고 항산화 기능성을 부여하는 유효한 공정임을 확인하였다.

• 한국항해항만학회지
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• 제34권6호
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• pp.493-501
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• 2010

부산 해운대구에서 사하구에 이르는 부산의 중심지역에서는 도시화의 진전 속에 어촌은 사실상 소멸하였으며, 인구밀집구역에 둘러싸인 17개 어항들이 주변지역과의 부조화 속에 방치되거나 고립되어 있는 상태이다. 그러나 이러한 도시화지역 내부의 어항은 해양도시로서의 아이덴티티를 부여하는 존재이며 그 활용여부에 따라 어항 자체 뿐 아니라 주변지역의 가치를 고양할 수 있는 잠재력을 가진 사회경제적 자원으로 삼을 수 있다는 것이 필자들의 기본적인 생각이다. 본 연구에서 우리는 중부산지역의 어항들을 대표적인 '도시형 어항'으로 정의하고, '도시'와 '어항'의 공존과 조화의 방향을 모색코자 하였으며, 이를 위해 중부산지역 17개 어항들의 어업생산기반과 주변환경 및 어항정비수요를 조사하고 어업인들과 지역주민들의 의견을 취합하여 도시와 어항의 조화로운 공존을 위한 도시형 어항정비의 중장기적 방향을 설정하는 것을 주된 목표로 하였다.

• Journal of Microbiology
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• 제43권3호
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• pp.257-259
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• 2005

Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.

• Archives of Plastic Surgery
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• 제42권5호
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• pp.614-618
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• 2015

Background Hand strength deficit following digital replantation is usually attributed to the mechanical deficiency of the replanted digit. Zone 1 replantation, however, should not be associated with any mechanical deficit, as the joint and tendon are intact. We evaluate shortterm motor functions in patients who have undergone single-digit zone 1 replantation. Methods A single-institution retrospective review was performed for all patients who underwent zone 1 replantation. Hand and pinch strengths were evaluated using standard dynamometers. Each set of measurements was pooled according to follow-up periods (within 1 month, 1 to 2 months, 2 to 3 months, and after 3 months). The uninjured hand was used as reference for measurements. Results The review identified 53 patients who had undergone zone 1 replantation and presented for follow-up visits. Compared to the uninjured hand, dynamometer measurements revealed significantly less strength for the hand with replanted digit at one month. The relative mean grip, pulp, and key pinch strength were 31%, 46%, and 48% of the uninjured hand. These three strength measurements gradually increased, with relative strength measurements of 59%, 70%, and 78% for 4-month follow up. Conclusions Despite the lack of joint or tendon injury, strength of the injured hand was significantly lower than that of the uninjured hand during the 4 months following replantation. Improved rehabilitation strategies are needed to diminish the short-term negative impact that an isolated zone 1 replantation has on the overall hand strength.

• 품질경영학회지
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• 제44권4호
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• pp.713-733
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• 2016

Purpose: The primary objective of this case study is to establish a COQ(Cost of Quality) management system for power generation industries. Key topics of this study include collecting COQ elements, their classifications, COQ computation formula, and determining COQ improvement projects. Results: A comprehensive set of COQ elements have been isolated for electric power generation companies. The COQ elements were classified in such a way that they could be managed according to the PAF model as well as the SIPOC diagram. This study showed that a systematic approach could be established for monitoring the COQ elements and using them in the process of improving quality competitiveness. Methods: The PAF(Prevention-Appraisal-Failure) model has been employed in the process of collecting COQ elements for a power generation company. All the cost of quality elements were first examined through an extensive review of articles and books in the field of quality. The cost elements were then refined and augmented by conducting a comparative study with international standards. The COQ elements have been verified by a group of quality managers and classified according to both the PAF model and the SIPOC diagram for better understanding in the entire organization. An improvement strategy has been also proposed by using a typical COQ level of power generation companies. Conclusion: The conventional PAF model was used in establishing a COQ management system for power generation industries. This case study illustrates the procedure about identification, classification and computation of quality costs, including selection of improvement projects. The system can be used not only for observing the current state of cost elements related to quality, but also for planning an improvement strategy using the ratio of cost classification.