• Title/Summary/Keyword: isolated set

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Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers

  • Kim, Sang-Woo;Kim, Sinil;Lee, Hyun-Jun;Park, Ju-Wan;Ro, Hyeon-Su
    • Mycobiology
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    • v.41 no.4
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    • pp.252-255
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    • 2013
  • Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.

Generalized Weyl's Theorem for Some Classes of Operators

  • Mecheri, Salah
    • Kyungpook Mathematical Journal
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    • v.46 no.4
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    • pp.553-563
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    • 2006
  • Let A be a bounded linear operator acting on a Hilbert space H. The B-Weyl spectrum of A is the set ${\sigma}_{B{\omega}}(A)$ of all ${\lambda}{\in}\mathbb{C}$ such that $A-{\lambda}I$ is not a B-Fredholm operator of index 0. Let E(A) be the set of all isolated eigenvalues of A. Recently in [6] Berkani showed that if A is a hyponormal operator, then A satisfies generalized Weyl's theorem ${\sigma}_{B{\omega}}(A)={\sigma}(A)$\E(A), and the B-Weyl spectrum ${\sigma}_{B{\omega}}(A)$ of A satisfies the spectral mapping theorem. In [51], H. Weyl proved that weyl's theorem holds for hermitian operators. Weyl's theorem has been extended from hermitian operators to hyponormal and Toeplitz operators [12], and to several classes of operators including semi-normal operators ([9], [10]). Recently W. Y. Lee [35] showed that Weyl's theorem holds for algebraically hyponormal operators. R. Curto and Y. M. Han [14] have extended Lee's results to algebraically paranormal operators. In [19] the authors showed that Weyl's theorem holds for algebraically p-hyponormal operators. As Berkani has shown in [5], if the generalized Weyl's theorem holds for A, then so does Weyl's theorem. In this paper all the above results are generalized by proving that generalizedWeyl's theorem holds for the case where A is an algebraically ($p,\;k$)-quasihyponormal or an algebarically paranormal operator which includes all the above mentioned operators.

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Modeling HRTFs for Customization (맞춤형 머리전달함수 구현을 위한 모델링 기법)

  • Shin, Ki-H.;Park, Young-Jin;Park, Yoon-Shik
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.11a
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    • pp.641-644
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    • 2005
  • This study reveals some recent attempt in modeling empirically obtained B&K HATS (Head and Torso Simulator) HRTFs (Head Related Transfer Functions) to Isolate parameters that stimulate lateral and elevation perception. Localization using non-individual HRTFs often yields poor performance in synthesizing virtual sound sources when applied to a group of individuals due to differences in size and shape of head, pinnae, and torso. For realization of both effective and efficient virtual audio it is necessary to develop a method to tailor a given set of non-individual HRTFs to fit each listener without measuring his/her HRTF set. Pole-zero modeling is applied to fit HRIRs (Head Related Impulse Responses) and modeling criterions for determining suitable number of parameters are suggested for efficient modeling. Horizontal HRTFs are modeled as minimum-phase transfer functions with appropriate ITDs (Interaural Time Delay) obtained from RTF (Ray Tracing Formula) to better fit the size of listener's head for usage in simple virtualizer algorithms without complex regularization processes. Result of modeling HRTFs in the median plane is shown and parameters responsible for elevation perception are isolated which can be referred to in the future study of developing customizable HRTFs.

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Influence of strong ground motion duration on reinforced concrete walls

  • Flores, Camilo;Bazaez, Ramiro;Lopez, Alvaro
    • Earthquakes and Structures
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    • v.21 no.5
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    • pp.477-487
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    • 2021
  • This study focuses on the influence of strong ground motion duration on the response and collapse probability of reinforced concrete walls with a predominant response in flexure. Walls with different height and mass were used to account for a broad spectrum of configurations and fundamental periods. The walls were designed following the specifications of the Chilean design code. Non-linear models of the reinforced concrete walls using a distributed plasticity approach were performed in OpenSees and calibrated with experimental data. Special attention was put on modeling strength and stiffness degradation. The effect of duration was isolated using spectrally equivalent ground motions of long and short duration. In order to assess the behavior of the RC shear walls, incremental dynamic analyses (IDA) were performed, and fragility curves were obtained using cumulative and non-cumulative engineering demand parameters. The spectral acceleration at the fundamental period of the wall was used as the intensity measure (IM) for the IDAs. The results show that the long duration ground motion set decreases the average collapse capacity in walls of medium and long periods compared to the results using the short duration set. Also, it was found that a lower median intensity is required to achieve moderate damage states in the same medium and long period wall models. Finally, strength and stiffness degradation are important modelling parameters and if they are not included, the damage in reinforced concrete walls may be greatly underestimated.

A Study on DC Traction Power Supply System Using PWM Converter (PWM컨버터를 적용한 경전철 전력공급시스템에 관한 연구)

  • Kim, Joorak;Park, Chang-Reung;Park, Kijun;Kim, Joo-Uk
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.29 no.4
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    • pp.250-254
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    • 2016
  • Currently, power conversion system which converts AC to DC Power is applied in domestic urban railway. The diode rectifier is used in most of them. However the diode rectifier can not control the output voltage and can not regenerate power as well. On the other hand, PWM (pulse width modulation) converter using IGBT (isolated gate bipolar transistor) can control output voltage, allowing it to reduce the output voltage drop. Moreover the Bi-directional conduction regenerates power which does not require additional device for power regeneration control. This paper compared the simulation results for the DC power supply system on both the diode rectifier and the PWM converter. Under the same load condition, simulation circuit for each power supply system was constructed with the PSIM (performance simulation and modeling tool) software. The load condition was set according to the resistance value of the currently operating impedance of light rail line, and the line impedance was set according to the distance of each substations. The train was set using a passive resistor. PI (proportional integral) controller was applied to regulate the output voltage. PSIM simulation was conducted to verify that the PWM Converter was more efficient than the diode rectifier in DC Traction power supply system.

Molecular Analysis of Colonized Bacteria in a Human Newborn Infant Gut

  • Park Hee-Kyung;Shim Sung-Sub;Kim Su-Yung;Park Jae-Hong;Park Su-Eun;Kim Hak-Jung;Kang Byeong-Chul;Kim Cheol-Min
    • Journal of Microbiology
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    • v.43 no.4
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    • pp.345-353
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    • 2005
  • The complex ecosystem of intestinal micro flora is estimated to harbor approximately 400 different microbial species, mostly bacteria. However, studies on bacterial colonization have mostly been based on culturing methods, which only detect a small fraction of the whole microbiotic ecosystem of the gut. To clarify the initial acquisition and subsequent colonization of bacteria in an infant within the few days after birth, phylogenetic analysis was performed using 16S rDNA sequences from the DNA iso-lated from feces on the 1st, 3rd, and 6th day. 16S rDNA libraries were constructed with the amplicons of PCR conditions at 30 cycles and $50^{\circ}C$ annealing temperature. Nine independent libraries were produced by the application of three sets of primers (set A, set B, and set C) combined with three fecal samples for day 1, day 3, and day 6 of life. Approximately 220 clones ($76.7\%$) of all 325 isolated clones were characterized as known species, while other 105 clones ($32.3\%$) were characterized as unknown species. The library clone with set A universal primers amplifying 350 bp displayed increased diversity by days. Thus, set A primers were better suited for this type of molecular ecological analysis. On the first day of the life of the infant, Enterobacter, Lactococcus lactis, Leuconostoc citreum, and Streptococcus mitis were present. The largest taxonomic group was L. lactis. On the third day of the life of the infant, Enterobacter, Enterococcus faecalis, Escherichia coli, S. mitis, and Streptococcus salivarius were present. On the sixth day of the life of the infant, Citrobacter, Clostridium difficile, Enterobacter sp., Enterobacter cloacae, and E. coli were present. The largest taxonomic group was E. coli. These results showed that microbiotic diversity changes very rapidly in the few days after birth, and the acquisition of unculturable bacteria expanded rapidly after the third day.

Isolation and Structural Determination of a Complete Set of Intact Bacteriochlorophyll-d Homologs and Isomers from Green Sulfur Bacterium Chlorobium vibrioforme and Their Aggregation Properties in Hydrophobic Solvents

  • Mizoguchi, Tadashi;Saga, Yoshitaka;Tamiaki, Hitoshi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.344-346
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    • 2002
  • Eight intact bacteriochlorophyll (BChl)-d homologs and isomers were isolated from a strain of green sulfur bacterium Chlorobium vibrioforme. All the molecular structures of the BChl-d components were fully determined by a combination of mass spectrometry and $^1$H-NMR spectroscopy. The aggregation behavior of the isomerically pure BChls-d in hydrophobic organic solvents was examined with respect to the stereoisomeric configuration at the C3$^1$ position as well as the bulkiness of the C8 and C12 side-chains by using electronic- absorption spectroscopy.

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Speech Recognition in Car Noise Environments Using Multiple Models Based on a Hybrid Method of Spectral Subtraction and Residual Noise Masking

  • Song, Myung-Gyu;Jung, Hoi-In;Shim, Kab-Jong;Kim, Hyung-Soon
    • The Journal of the Acoustical Society of Korea
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    • v.18 no.3E
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    • pp.3-8
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    • 1999
  • In speech recognition for real-world applications, the performance degradation due to the mismatch introduced between training and testing environments should be overcome. In this paper, to reduce this mismatch, we provide a hybrid method of spectral subtraction and residual noise masking. We also employ multiple model approach to obtain improved robustness over various noise environments. In this approach, multiple model sets are made according to several noise masking levels and then a model set appropriate for the estimated noise level is selected automatically in recognition phase. According to speaker independent isolated word recognition experiments in car noise environments, the proposed method using model sets with only two masking levels reduced average word error rate by 60% in comparison with spectral subtraction method.

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Noise Robust Automatic Speech Recognition Scheme with Histogram of Oriented Gradient Features

  • Park, Taejin;Beack, SeungKwan;Lee, Taejin
    • IEIE Transactions on Smart Processing and Computing
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    • v.3 no.5
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    • pp.259-266
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    • 2014
  • In this paper, we propose a novel technique for noise robust automatic speech recognition (ASR). The development of ASR techniques has made it possible to recognize isolated words with a near perfect word recognition rate. However, in a highly noisy environment, a distinct mismatch between the trained speech and the test data results in a significantly degraded word recognition rate (WRA). Unlike conventional ASR systems employing Mel-frequency cepstral coefficients (MFCCs) and a hidden Markov model (HMM), this study employ histogram of oriented gradient (HOG) features and a Support Vector Machine (SVM) to ASR tasks to overcome this problem. Our proposed ASR system is less vulnerable to external interference noise, and achieves a higher WRA compared to a conventional ASR system equipped with MFCCs and an HMM. The performance of our proposed ASR system was evaluated using a phonetically balanced word (PBW) set mixed with artificially added noise.

Identification and Molecular Characterization of Insecticidal cryl-type Genes from Bacillus thuringiensis 2385-1

  • Li, Ming-Shun;Park, Jae-Young;Roh, Jong-Yul;Shim, Hee-Jin;Boo, Kyung-Saeng;Je, Yeon-Ho
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.114-115
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    • 2003
  • A Bacillus thuringiensis isolate, Bt 2385-1, which showed toxicity to lepidopteran, was isolated from Korean soil sample and characterized. PCR-RFLP showed that this isolate contains two novel cryl-type crystal protein genes. In this study, we designed cryl-type specific primer set (ATG1-F and N400-R) to clone the toxic domain of the all cryl-type genes. The two novel rlyl-type toxin genes in addition to crylJal gene were cloned and sequenced. (omitted)

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