Proceedings of the Korean Society of Sericultural Science Conference (한국잠사학회:학술대회논문집)

Korean Society of Sericultural Science (한국잠사학회)
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Identification and Molecular Characterization of Insecticidal cryl-type Genes from Bacillus thuringiensis 2385-1

  • Li, Ming-Shun (School of Agricultural Biotechnology, Seoul National University) ;
  • Park, Jae-Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University) ;
  • Shim, Hee-Jin (School of Agricultural Biotechnology, Seoul National University) ;
  • Boo, Kyung-Saeng (School of Agricultural Biotechnology, Seoul National University) ;
  • Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2003.10.01
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Abstract

A Bacillus thuringiensis isolate, Bt 2385-1, which showed toxicity to lepidopteran, was isolated from Korean soil sample and characterized. PCR-RFLP showed that this isolate contains two novel cryl-type crystal protein genes. In this study, we designed cryl-type specific primer set (ATG1-F and N400-R) to clone the toxic domain of the all cryl-type genes. The two novel rlyl-type toxin genes in addition to crylJal gene were cloned and sequenced. (omitted)

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