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http://dx.doi.org/10.5941/MYCO.2013.41.4.252

Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers  

Kim, Sang-Woo (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
Kim, Sinil (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
Lee, Hyun-Jun (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
Park, Ju-Wan (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
Publication Information
Mycobiology / v.41, no.4, 2013 , pp. 252-255 More about this Journal
Abstract
Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.
Keywords
Fungal pathogens; ITS primer; Mushroom; Pleurotus eryngii;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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