• 대한약리학회지
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• 제14권1_2호
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• pp.41-46
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• 1978

The effects of sodium taurocholate(STC) and sodium deoxycholate(SDC) on cardiac function were examined by using isolated atria of rabbit and guinea pig and heart of anesthetized frog. Also the antiarrythmic action of STC and SDC on atrial arrhythmias induced by epinephrine or ouabain was studied. The results were following. The cholates exhibited a slight decrease in rate and contractile amplitude of the isolated rabbit atria. The cholates abolished partially the spontaneous arrhythmic occurring in isolated rabbit and guinea pig atria but no effect on the atrial arrhythmia induced by ouabain and epinephrine was observed. Concomitant administration of cholates with ouabain produced a marked prolongation of atrial arrhythmia in comparison to that of ouabain alone in both isolated rabbit and guinea pig atria. The cholates exhibited a marked prolongation in ventricular arrhythmia and cardiac arrest time in comparison to that of ouabain treatment. However, the combined treatment with cholates and ouabain produced a slight prolongation in comparison to that of ouabain alone in the heart of anesthetized frog. The above results suggest that cholates have a slight antiarrythmic effect on the heart but this effectiveness is different from those of propranolol that is non-selective antiarrhythmic drug.

• Journal of Chest Surgery
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• 제18권4호
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• pp.563-568
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• 1985

Two cases of situs inversus totalis with isolated congenital heart disease were treated at department of cardiothoracic surgery, Chungnam National University Hospital. Sixteen months old girl was operated under diagnosis of PDA with mirro-image dextrocardia by triple ligation. Fifteen months old girl was managed under diagnosis of VSD with mirro-image dextrocardia by simple closure of VSD with extracorporeal circulation. The former had smooth postoperative course, but the latter was died of asphyxia due to vomitus on postoperative second day

• 한국환경보건학회지
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• 제5권1호
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• pp.71-75
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• 1978

Distribution of Vibrio parahaemolyticus in the Tegillaria granosa Linne in Costal area Inchun. The survey has been done chiefly from the Inchun Sea ports during 5 months from Oct. 1974 to May 1975. 1. The number of Vibrio parahaemolyticus isolated 17(13.8%) of 117 specimens from Anadara Subcrenata. 2. The number of Vibrio alginolyticus isolated 92 strains of 119 specimens from Tegillaria granosa Linne. 3. Bacteriological characterigation such as morphological, ecological, biochemical results of 17 isolated strain has been identified the category of typical Vibrio parahaemolyticus differentiation. 4. Most of Kanagawa positive strains was positive in Greig test, and considerable number of Kanagawa negative strains was also positive. The result of Greig test was almost the same between cultures in brain heart infusion broth and brain heart infusion agar. 5. Kanagawa positive strains showed same lethal activity in inoculated mice into peritoreal cavity as compared with Kanagawa negative strain.

• 생약학회지
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• 제18권4호
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• pp.241-248
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• 1987

Woohwangchungsim-Won has been widely used for the treatment of appoplexy, hypertension, arteriosclerosis, insomnia and cerebrovascular accident, etc in oriental hospital and pharmacy. In order to investigate the efficacy of Woohwangchungsim-Won, the water extract of it were bioassayed for isolated ileum, blood vessels, blood pressure, heart and diuresis. The results of this studies were as follows; Spontaneous motilities of isolated ileum of mice were strongly suppressed, and contraction of isolated ileum of mice and guinea-pigs induced by acetylcholine chloride, barium chloride and histamine were inhibited. Vaso-dilating action due to vascular smooth muscle relaxation in frogs and rabbits, and hypotensive action in anesthetized rabbits were noted. Negative inotropic action on the isolated frog heart and diuretic effect in rabbits were shown.

• 대한약리학회지
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• 제22권2호
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• pp.105-114
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• 1986

본 실험실에서는 민간요법으로 말라리아와 성병등의 치료제로 사용되어온 회양목(Buxus microphylla var. koreana Nakai)에서 steroid성 alkaloid인 cyclobuxine D와 nonalkaloid인 buxuletin을 분리하였다. Buxuletin의 가토에 있어서 이뇨작용에 대해서는 본 실험실에서 보고한바 있으며 cyclobuxine D의 약리작용에 대한 보고는 지금까지 전무한 상태이다. 본 실험에서는 새로운 항부정맥 약물의 밭견의 일환으로 cyclobuxine D의 적출 개구리 심장에 대한 작용과 마취시킨 흰쥐의 ECG와 심박동수에 대한 작용을 관찰하였다. Cyclobuxine D는 적출 개구리 심장에 대해 용량의존적 심근 수축력 감소를 나타냈으며 흰쥐에 있어서 현저한 심박동수 감소를 나타냈다. Cyclobuxine D는 흰쥐의 ECG에 있어서 PR interval과 P ${\alpha}T$ interval을 연장시키며, QRS complex와 PR interval의 심박동수에 대한 보상치인 PRc에 대해서는 고용량에서는 연장시키나 그 작용이 현저하지 않다. 이 결과로 보아 cyclobuxine D는 흰쥐의 ECG에서 A-V conduction과 ventricular depolarization에 주로 관여하는 것으로 사료 되며 이는 기존의 antiarrhythmic drugs과 비교해 볼 때 quinidine sulfate와 유사성이 있다고 추정된다.

• Journal of Chest Surgery
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• 제23권6호
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• pp.1074-1083
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• 1990

This study was evaluated the metabolic, physiologic and histologic effects of myocardial protection of verapamil[isoveratril]on isolated rat hearts to 90 minutes of ischemic arrest. Heart was perfused with a modified Kreb’s Henseleit bicarbonate buffer with glucose and arrested with retrograde coronary perfusion by glucose insulin[GI], potassium and verapamil. Mean aortic systolic pressure, heart rate, coronary flows were measured and morphologic changes were examined during working heart perfusion. Perfusion and arrest were controlled four groups subjected 60 isolated rat hearts. Four groups hearts reperfused during 40 minutes after 90 minutes global ischemia for physiologic recovery. 15 hearts of four groups were assayed to histological morphologic changes. GI treated hearts recovered less than 28% of function and changed more than 80% of mitochondria of control group. Verapamil hearts[0.2, 0.1 gm/kg] recovered more than 88% of function and permitted the maintenance of continuous cellular level of Serum Glutamic Oxalaxetate Transaminase[SGOT], but declined 28% of Phosphate Kinase[CP], GI treated heart showed widespread evidence of extensive damage of mitochondria. The damage was that interstitial huge edema are present and there was contraction band formation within the swollen cells. The verapamil and potassium group were not found morphologic change compared with control group. Their functions were shown that metabolic and physiologic action of verapamil-group lasted 20 minutes longer than potassium group.

• Journal of Chest Surgery
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• 제28권6호
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• pp.549-556
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• 1995

We investigated the effects of aprotinin, a protease inhibitor, on isolated rat heart subjected to cardioplegia and global ischemia for 4 hours and then reperfused for 40 minutes. Before ischemia, hearts were perfused with either aprotinin 1x105KIU/L[Aprotinin group,n=8 or no aprotinin[control group,n=8 added to Krebs-Henseleite solution for 30 minutes. Hemodynamic and biochemical parameters such as heart rate, LVP, dP/dt, coronary flow and creatine kinase were measured before cardioplegia and after reperfusion 10,20,30,40 minutes. After completion of experiment, wet and dry heart weight were measured for tissue water and water content evaluation. On reperfusion, recovery of LVP of aprotinin group at each time point was significantly better than that of control group[p<0.05 , and of dP/dt at reperfusion 40 minutes[p=0.034 . No statistically significant differences in heart rate, coronary flow and CK were observed between the two groups, but aprotinin group seemed to have better recovery. No significant differences in tissue water and water content were observed between the two group.These results suggest that pretreatment of aprotinin is effective in myocardial preservation in prolonged hypothermic ischemia and reperfusion.

• 대한수의학회지
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• 제27권1호
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• pp.35-40
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• 1987

Higenamine, a benzyltetrahydroisopuinoline analog isolated from aconite tuber, has potent isotropic action. Recent studies suggest it may have beta receptor agonistic property in that its inotropic action is blocked by propranolol in isolated rabbit heart. However, no study has been carried out on other organs than heart. Higenamine is expected to have pharmacological actions on smooth muscle on the ground that it has catecholamine moiety and tetrahydrosioquinoline nucleus in its chemical structure, both of which are well known to have smooth muscle relaxation effects. Therefore present study was aimed at determining whether higenamine has bronchodilating effect in isolated guinea pig trachea smooth muscle rich in adrenergic beta receptor and if any, it has agonistic effect on beta receptor. The results were summarized as follows : 1. Higenamine had remarkable bronchodilating effect in guinea pig tracheal smooth muscle in a dose-dependent manner. 2. Bronchodilator effect of higenamine in isolated guinea pig tracheal smooth muscle was blocked competitively by propranolol. The $pD_2$ value of higenamine in isolated guinea pig tracheal smooth muscle was 5.65 and the $pA_2$ value of propranolol against higenamine in the same preparation was 7.97.

• Journal of Chest Surgery
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• 제22권6호
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• pp.887-893
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• 1989

Donor availability is a major limiting factor in heart transplantation. Prolonging donor heart preservation would facilitate distant heart procurement. The setup used was the isolated retrograde perfused nonworking rabbit heart model and 4 hours of preservation at 2oC. And the electron microscopic findings of the myocardium were evaluated after reperfusion for 5 minutes. The following three groups [each group, n=4] were prepared: Group I: the heart was arrested with the St. Thomas Hospital solution [STH] and stored in Ringers lactate solution [RLS]; Group II: the heart was arrested with STH and stored in Modified Collins-Sachs solution [MCS]; Group g: the heart was arrested with and stored in MCS. The result was the most severe myocardial injury in the Group III on electron microscopic study.

• 대한수의학회지
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• 제41권4호
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• pp.497-503
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• 2001

Evidence from the last 10 years have been suggested that melatonin mainly produce a depressant effect on the cardiac system, but we found an activating effect of melatonin on heart rate in this research. To determine the hypothesis that melatonin has dual effects on physiological behaviour of cardiac system, we investigated the effects of melatonin on heart rate in isolated rat atria and anesthetized rats. Regardless of concentration, melatonin produced bradycardia in the 84 cases of 148 experiments (57 %) and tachycardia in the 64 cases of 148 experiments (43 %). And in atrium, melatonin produced a decrease automaticity in 52 cases of 86 experiments (60 %) and increase automaticity in 40 % (34/86 cases). Also, these effects are not significnat relationship with concetration of melatonin. The melatonin-induced bradycardia in vivo was inhibited by pretreatment of atropine or bilateral cervical vagotomy. Also, in isolated atrium the melatonin-induced decrease in automaticity was inhibited by pretreatment of atropine. These melatonin-induced responses were potenitated by pretreatment of propranolol. The melatonin-induced tachycardia in vivo was inhibited by pretreatment of propranolol, nifedipine or bilateral cervical vagotomy, but not by pretreatment of atropine. The melatonin-induced incease in automaticity in isolated atrium was converted to decrease in automaticity by pretreatment of propranolol. In addition, the change in heart rate caused by adrenoceptor agonists was inhibited by pretreatment of melatonin. These results indicate that melatonin-induced bradycardia may be related to a muscarinic receptor activation and melatonin-induced tachycardia may be related to a $\beta$-adrenoceptor stimulation.