• 한국지하수토양환경학회지:지하수토양환경
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• 제20권1호
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• pp.56-64
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• 2015

Acid mine drainage occurrence is a serious environmental problem by mining industry, it usually contains high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of the greatest concern. An indigenous plant extract was used to produce calcium carbonate from Canavalia ensiformis as effective biomaterial, and its ability to form the calcium carbonate under stable conditions was compared to that of purified urease. X-ray diffraction and scanning electron microscopy were employed to elucidate the mechanism of calcium carbonate formation from the crude plant extracts. The results revealed that urease in the plant extracts catalyzed the hydrolysis of urea in liquid state cultures and decreased heavy metal amounts in the contaminated soil. The heavy metal amounts were decreased in the leachate from the treated mine soil; 31.7% of As, 65.8% of Mn, 50.6% of Zn, 51.6% of Pb, 45.1% of Cr, and 49.7% of Cu, respectively. The procedure described herein is a simple and beneficial method of calcium carbonate biomineralization without cultivation of microorganisms or further purification of crude extracts. This study suggests that crude plant extracts of Canavalia ensiformis have the potential to be used in place of purified forms of the enzyme during remediation of heavy metal contaminated soil.

• 아시안잔디학회지
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• 제11권2호
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• pp.89-95
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• 1997

Soil chemical properties were investigated to elucidate vertical movement of mineral nutrients in a fairway slope of 27 year-old golf course. Soil samples were taken at every 10cm depth to 4Ocm on 4 sites 20m apart each along two parallel lines 60m apart on the slope(15˚) in August. Accord-ing to the similarity of vertical distribution pattern they could be classified into 6 distinctive groups(magnesium, nitrate, phosphorus, ammonium, manganese and copper) and the slope tended to affect the vertical movement of minerals. The contents of Ca and Mg increased with depth while NO$_3$-N and Zn decreased and correlated positively. Soil pH showed significant positive correlation with depth, Ca and Mg. Phosphorus content was highest in 10~20cm depth and decreased resulting in relatively high content in 30~40cm depth of the bottom site of slope. Iron distribution pattern was similar to phosphorus. Potassium and $NH_4$showed various(irregular) patterns. Mn was highest in the deep layer at the lower sites of slope but it was reverse at the upper sites and negatively correlated with Fe. Cu content incresed with depth and heighest in 20~30cm depth. Electroconductivity showed significant positive correlation with $NO_3$-N. Magnesium, cal-cium and copper seem to be prone to defficiency due to fast leaching, and nitrate and phosphorus prone to excess problem. Preventive measures on acidification of surface soil should be taken.

• Nutrition Research and Practice
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• 제11권1호
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• pp.64-69
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• 2017

BACKGROUND/OBJECTIVES: This study was performed to measure fat-soluble vitamins and minerals in breast milk of Korean lactating mothers who exclusively breastfed their babies. SUBJECTS/METHODS: Breast milk samples were collected from 334 mothers. Concentrations of retinol and ${\alpha}$-tocopherol were analyzed by high performance liquid chromatography ultraviolet spectrometry while concentrations of minerals were measured by inductively coupled plasma optical emission spectrometry. RESULTS: Retinol and ${\alpha}$-tocopherol contents of breast milk were $39.58{\pm}19.64{\mu}g/dL$ and $0.23{\pm}0.13mg/dL$, respectively. Average sodium, potassium, calcium, phosphorus, and magnesium levels in breast milk were $11.11{\pm}5.16$, $38.56{\pm}9.01$, $27.87{\pm}6.10$, $13.56{\pm}3.30$, and $3.05{\pm}0.65mg/dL$, respectively. Contents of trace elements such as iron, zinc, copper, and manganese were $40.26{\pm}46.21$, $98.40{\pm}62.47$, $24.09{\pm}9.03$, and $0.90{\pm}1.63{\mu}g/dL$, respectively. Fat-soluble vitamin concentration was positively correlated with total fat in milk samples, but no significant differences were observed in levels of retinol, ${\alpha}$-tocopherol, or minerals based on whether or not lactating women were taking dietary supplements. CONCLUSIONS: Micronutrient contents of breast milk samples from Korean lactating women were comparable to those of other nations. Retinol and ${\alpha}$-tocopherol levels were correlated and also with total fat in breast milk.

• 광물과 암석
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• 제33권4호
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• pp.377-389
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• 2020

본 연구에서는 낙동강수계의 안동댐 퇴적물에 대한 오염도 조사 및 평가를 수행하였다. 안동댐 상류 40 km까지 약 5 km 간격으로 호수의 중간, 좌, 우지점에서 성층기와 전도기로 구분하여 퇴적물 시료를 채취하였다. 일반 항목인 강열 감량, 총질소, 총인의 경우 오염도가 낮지만, 중금속의 경우 비소와 카드뮴의 오염도는 매우 높다. 지천 및 대조군에 비해 댐 내 퇴적물의 오염도가 높으며, 시료채취 시기별로는 성층기에 크롬, 구리, 납의 농도가 높고, 순환기에 아연의 농도가 높은 경향을 보인다. 상하류의 위치에 따른 농도 차이를 나타내는 중금속은 비소, 카드뮴, 망간, 아연이며 차이를 보이지 않는 항목은 크롬, 구리, 수은, 철, 납 등이다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1614-1621
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• 2013

The aim of this study was to investigate the effects of graded levels of montmorillonite, a constituent of clay, on performance, hematological parameters and bone mineralization in weaned pigs. One hundred and twenty, 35-d-old crossbred pigs (Duroc${\times}$Large White${\times}$Landrace, $10.50{\pm}1.20$ kg) were used in a 28-d experiment and fed either an unsupplemented corn-soybean meal basal diet or similar diets supplemented with 0.5, 1.0, 2.5 or 5.0% montmorillonite added at the expense of wheat bran. Each treatment was replicated six times with four pigs (two barrows and two gilts) per replicate. Feed intake declined (linear and quadratic effect, p<0.01) with increasing level of montmorillonite while feed conversion was improved (linear and quadratic effect, p<0.01). Daily gain was unaffected by dietary treatment. Plasma myeloperoxidase declined linearly (p = 0.03) with increasing dietary level of montmorillonite. Plasma malondialdehyde and nitric oxide levels were quadratically affected (p<0.01) by montmorillonite with increases observed for pigs fed the 0.5 and 1.0% levels which then declined for pigs fed the 2.5 and 5.0% treatments. In bone, the content of potassium, sodium, copper, iron, manganese and magnesium were decreased (linear and quadratic effect, p<0.01) in response to an increase of dietary montmorillonite. These results suggest that dietary inclusion of montmorillonite at levels as high as 5.0% does not result in overt toxicity but could induce potential oxidative damage and reduce bone mineralization in pigs.

• 한국토양비료학회지
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• 제47권6호
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• pp.391-397
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• 2014

Acid mine drainage occurrence is a serious environmental problem by mining industry; it usually contain high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of greatest concern. It causes mine impacted soil pollution with mining and smelting activities, fossil fuel combustion, and waste disposal. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a minei-mpacted area. All isolated bacterial strains were identified Sporosarcina pasteurii with more than 98% of similarity, therefore they were named Sporosarcina sp. KM-01, KM-07, and KM-12. The heavy metals detected from the collected mine soils containing bacterial isolates as Mn ($170.50mg\;kg^{-1}$), As ($114.05mg\;kg^{-1}$), Zn ($92.07mg\;kg^{-1}$), Cu ($62.44mg\;kg^{-1}$), and Pb ($40.29mg\;kg^{-1}$). The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS analyses showed that calcium carbonate was successfully produced and increased with time. To confirm the calcium carbonate precipitation ability, urease activity and precipitate weight were also measured and compared. These results demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals by mining activity.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.144-144
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• 2017

Micronutrients such as zinc (Zn), iron (Fe), manganese (Mn) have important roles for development and growth in plants but it also have roles in animals and humans. In previous studies, a Korean weedy rice, KH2J was selected to have tolerance to heavy metal, lead (Pb) compared with a cultivar, Milyang23. To identify QTLs for micronutrients concentration in grain, an F2 population (120 plants) were developed from a cross between KH2J and an indica rice cultivar, Milyang23. To measure the concentration of eight ions, Zn, Fe, Mn, Pb, calcium (Ca), copper (Cu), cadmium (Cd) and arsenic (As), grains were collected and digested with 65% nitric acid, and the ion contents were measured using inductively coupled plasma mass spectrometry. A total 27 putative quantitative trait loci (QTLs) were detected on 12 chromosomes by single point analysis and 22 putative QTLs were detected by composite interval mapping. The co-locations of QTL for Zn, Fe and Mn were observed on chromosome 5. The QTLs for Cd, Cu and Zn were co-localized on chromosome 10, and QTLs for Zn, As and Mn was on chromosome 12. The Zn concentration in F2 generation showed significant correlation with concentrations of As (r = -0.4), Cu (r = 0.5) and Fe (r = 0.2) (P < 0.01). Also, the Ca concentration was significantly related with Mn and Fe concentrations (P < 0.01). Fine mapping of these QTLs is underway to analyze their functional relationship.

• Journal of Microbiology
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• 제45권2호
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• pp.105-112
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• 2007

The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0 %), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.

• Asian-Australasian Journal of Animal Sciences
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• 제8권3호
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• pp.249-254
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• 1995

The effects of bentonite (B) on rumen protozoal population and rumen fluid characteristics of sheep fed palm kernel cake (PKC) were studied for a period of 21 days. Two groups, each comprising two sheep were fed either PKC or PKC + B ad libitum A third group was left at pasture. Rumen fluid was sampled through a rumen cannula three times daily from all animals. Palm kernel cake contained 16% crude protein, 1 % crude fat and high amounts of copper, zinc, iron and manganese. Protozoal population in the rumen fluid decreased significantly (p < 0.05) after the onset of feeding PKC or PKC + B. However, sheep given bentonite supplementation at 2% of the dietary dry matter, maintained higher protozoal densities ($15{\times}10^4/ml$) when compared to animals fed only PKC ($8{\times}10^4/ml$). With both diets, the protozoa were mainly of the small entodinia species. Animals at pasture had higher protozoal population ($47{\times}10^4/ml$) with varying species of entodiniomorphids and holotrichs. Rumen fluid pH and ammonia concentration was significantly (p < 0.05) higher in animals at pasture compared to animals fed PKC or PKC + B. Volatile fatty acid concentration was significantly (p < 0.05) lower in animals fed PKC when compared to animals at pasture. There was a shift in fermentation pattern in animals fed PKC or PKC + B towards a lower acetate; and higher propionate, isovalerate and valerate. Studies in vitro also showed the positive effect of bentonite on protozoal numbers.

• 원예과학기술지
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• 제34권4호
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• pp.549-556
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• 2016

본 연구에서 제주의 화산회토와 비화산회토에서 난지형 마늘인 남도 마늘과 대서 마늘을 재배하여 토양 및 마늘의 성분을 비교 분석하였다. 남도 마늘을 재배한 화산회토양에서는 전기전도도 및 가용성 황 함량이 높았으며 대서 마늘을 재배한 화산 회토양에서는 유기물함량 및 총 질소함량이 높게 나타났다. 남도 마늘을 재배한 비화산회토의 경우 가용성 인 함량이 높게 나타났다. 화산회토에서 재배한 남도 마늘의 경우 가용성고형물 및 알리신 함량이 높았으며, 화산회토에서 재배한 대서 마늘의 경우 환원당 함량이 높게 나타났다. 또한 제주에서 재배된 마늘의 대량원소 함량은 칼륨이 가장 높았으며 다음으로 황, 마그네슘, 칼슘 및 나트륨 순이었으며 미량원소의 경우 철, 아연, 망간, 구리 순이었다. 이러한 결과는 고품질 마늘 생산을 목표로 하는 재배농가들에게 유용한 정보를 제공하게 될 것이다.