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http://dx.doi.org/10.7857/JSGE.2015.20.1.056

Bioremediation of Heavy Metal Contaminated Mine Wastes using Urease Based Plant Extract  

Roh, Seung-Bum (Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM))
Park, Min-Jeong (Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM))
Chon, Chul-Min (Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM))
Kim, Jae-Gon (Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM))
Song, Hocheol (Department of Environment and Energy, Sejong University)
Yoon, Min-Ho (Department of Bio Environmental Chemistry, Chungnam National University)
Nam, In-Hyun (Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM))
Publication Information
Journal of Soil and Groundwater Environment / v.20, no.1, 2015 , pp. 56-64 More about this Journal
Abstract
Acid mine drainage occurrence is a serious environmental problem by mining industry, it usually contains high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of the greatest concern. An indigenous plant extract was used to produce calcium carbonate from Canavalia ensiformis as effective biomaterial, and its ability to form the calcium carbonate under stable conditions was compared to that of purified urease. X-ray diffraction and scanning electron microscopy were employed to elucidate the mechanism of calcium carbonate formation from the crude plant extracts. The results revealed that urease in the plant extracts catalyzed the hydrolysis of urea in liquid state cultures and decreased heavy metal amounts in the contaminated soil. The heavy metal amounts were decreased in the leachate from the treated mine soil; 31.7% of As, 65.8% of Mn, 50.6% of Zn, 51.6% of Pb, 45.1% of Cr, and 49.7% of Cu, respectively. The procedure described herein is a simple and beneficial method of calcium carbonate biomineralization without cultivation of microorganisms or further purification of crude extracts. This study suggests that crude plant extracts of Canavalia ensiformis have the potential to be used in place of purified forms of the enzyme during remediation of heavy metal contaminated soil.
Keywords
Urease; Plant extract; $CaCO_3$ precipitation; Heavy metal; Soil remediation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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