• 제목/요약/키워드: iridovirus

검색결과 59건 처리시간 0.017초

담수관상어 5종에서의 iridoviruses 검출과 분포 분석 (Detection and distribution of iridoviruses in five freshwater ornamental fish species)

  • 정현도;류지효;정준범;김호열;전려진;조혜진;이준우
    • 한국어병학회지
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    • 제19권3호
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    • pp.197-206
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    • 2006
  • 2004~2005년까지 수입산 pearl gourami (Trichogaster leeri), dwarf gourami (Colisa lalia), silver gourami (Trichogaster microlepis), blue gourami (Trichogaster trichopterus sumatranus) 그리고 freshwater angelfish (Pterophyllum scalare) 등 5 어종을 대상으로 iridovirus 감염 실태를 PCR 방법을 사용하여 조사하였다. 채집한 56 samples 중 11 samples에서 PCR 양성 반응이 관찰되었고, pearl gourami에서 31.8% (7/22)로 가장 높은 감염 빈도를 나타내었다. 그 다음으로 dwarf gourami, blue gourami, silver gourami 순으로 나타났으며, freshwater angelfish에서는 iridovirus가 검출되지 않았다. 자연 감염된 관상어종의 비장으로부터 iridovirus의 핵산을 분리하여 검출한계 PCR 방법으로 viral DNA의 양을 비교하였으며, dwarf gourami에 비해 pearl gourami에서 더 많은 viral DNA가 검출되는 것을 확인하였다. 자연 감염이 확인된 pearl gourami groups는 실험실 환경에서 100% 폐사하였고, 폐사어 중 57% 이상이 PCR 양성 반응을 나타내었다. PstⅠfragment의 염기서열 비교에서 pearl gourami와 dwarf gourami에서 분리한 iridoviruses는 중국의 mandarinfish (Siniperca chuatsi)에서 분리된 infectious spleen and kidney necrosis virus (ISKNV)와 99% 이상의 매우 높은 상동성을 나타내었다.

Bacillus polyfermenticus KJS-2에서 발효된 콩의 돌돔에 대한 이리도바이러스 및 병원성균에 대한 예방효과 (Soybeans Fermented with Bacillus po/yfermenticus KJS-2 Protects Oplegnathus fasciatus from Iridovirus and Pathogenic Bacterial Infection)

  • 김강민;나해춘;박정희;강재선
    • 생명과학회지
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    • 제19권6호
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    • pp.720-727
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    • 2009
  • Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2)를 이용하여 발효된 콩(BP2FS)은 8가지의 병원성균에 대해 항균활성을 가졌다. BP2FS는 돌돔의 배양에 있어 사료첨가제로 사용되었다. 돌돔의 배양시 BP2FS를 투여한 군($6{\times}10^{4}$ cfu $g^{-1}$ 사료)과 투여하지 않은 군으로 나누었으며, 투여한 군은 120일 동안 하루에 두 번씩 투여 하였다. 120일 후에 투여한 군의 돌돔의 몸무게는 67.29${\pm}$12.62 g이었으며 그렇지 않은 군의 몸무게는 56.56${\pm}$8.21 g으로 나타났다. 생존율은 투여한 군(80%)과 투여하지 않은 군(40%)이었다. 폐사한 돌돔의 축적된 양은 투여한 군(18.95%)과 투여하지 않은 군(60.98%)이었다. BP2FS를 투여한 군에서 B. polyfermenticus KJS-2를 돌돔의 장내에서 검출한 결과 $1.04{\times}10^{4}$ cfu $g^{-1}$으로 나타났다. BP2FS를 투여 하지 않은 군의 돌돔에서는 iridovirus에 감염되었을 경우 나타나는 전형적인 증상인 비장과 간에서 비대세포가 관찰 되었다. 이 결과들은 BP2FS는 돌돔의 이리도바이러스에 대한 감염 시 예방효과를 가져온다 할 수 있다.

Polymerase chain reaction - restriction fragment length polymorphism을 이용한 바이러스성 어류 질병 진단 (Diagnosis of viral fish diseases by polymerase chain reaction - restriction fragment length polymorphism)

  • 김명석;박신후;조미영;김진우;박명애
    • 한국어병학회지
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    • 제21권3호
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    • pp.181-188
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    • 2008
  • Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to detect and identify four fish viruses, fish iridovirus, viral hemorrhagic septicaemia virus (VHSV), viral nervous necrosis virus (VNNV), hirame rhabdovirus (HRV). Four viruses were detected by PCR with each specific primers. Identification of iridovirus was achieved by digesting the PCR amplified fragment with a restriction enzyme ApaⅠ. It was possible to distinguish positive from false positive PCR amplicons of VHSV by RFLP of PstⅠ or HindⅢ restriction enzymes. VNNV was identified using RFLP of BamHⅠrestriction enzyme and HRV was identified by XbaⅠ restriction enzyme. This approach can be used for more rapid, simple and specific diagnosis of fish viral diseases.

수온별 Red Sea Bream Iridovirus (RSIV) 인위감염에 따른 돌돔의 누적폐사 및 Heat Shock Protein (HSP) 70의 동정 (Cumulative Mortality in Striped Beakperch, Oplegnathus fasciatus Infected with Red Sea Bream Iridovirus (RSIV) at Different Water Temperature and Identification of Heat Shock Protein 70)

  • 김석렬;정병문;정성주;키타무라 신이치;김두운;김도형;오명주
    • 한국어병학회지
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    • 제21권1호
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    • pp.13-20
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    • 2008
  • 본 연구에서는 RSIV의 수온에 따른 병원성을 규명하기 위한 연구의 일환으로 수온별로 RSIV를 인위적으로 감염시킨 돌돔의 누적폐사를 관찰하고, 다른 온도에서 사육된 돌돔의 온도 스트레스 단백질인 HSP이 관여하는지를 확인하고자, 돌돔의 HSP 유전자의 염기서열 일부를 밝혔다. 각온도별 감염실험에서 17°C와 20°C의 경우 폐사가 발생하지 않았으나, 25°C 및 27°C 수조에서는 각각 90%와 80%의 누적 폐사율을 보였다. 돌돔에서 확인된 HSP 유전자의 일부는 넙치에서 확인된 HSP70와 97%의 homology를 나타내 돌돔의 HSP70으로 판단되었다.

Expression Analysis of Interferon-Stimulated Gene 15 in the Rock Bream Oplegnathus fasciatus against Rock Bream Iridovirus (RSIV) Challenge

  • Kim, Kyung-Hee;Yang, In Jung;Kim, Woo-Jin;Park, Choul-Ji;Park, Jong-Won;Noh, Gyeong Eon;Lee, Seunghyung;Lee, Young Mee;Hwang, Hyung Kyu;Kim, Hyun Chul
    • 한국발생생물학회지:발생과생식
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    • 제21권4호
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    • pp.371-378
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    • 2017
  • Interferon-stimulated gene 15 (ISG15) is known to interfere with viral replication and infection by limiting the viral infection of cells. Interferon-stimulated gene 15 (ISG15) interferes with viral replication and infectivity by limiting viral infection in cells. It also plays an important role in the immune response. In this study, tissue-specific expression of ISG15 in healthy rock bream samples and spatial and temporal expression analysis of rock bream ISG15 (RbISG15) were performed following rock bream iridovirus (RSIV) infection. RbISG15 expression was significantly higher in the eye, gill, intestine, kidney, liver, muscle, spleen, and stomach, but low in the brain. There were particularly high levels of expression in the liver and muscle. RbISG15 expression was also examined in several tissues and at various times following RSIV infection. ISG15 expression increased within 3 h in the whole body and decreased at 24 h after infection. In addition, temporal expression of several tissues following RSIV infection showed a similar pattern in the muscle, kidney, and spleen, increasing at 3 h and decreasing at 72 h. These results suggest that ISG15 plays an important role in the immune response of rock bream. Overall, this study characterizes the response of RbISG15 following RSIV infection.

Transcriptional profiles of rock bream iridovirus (RBIV) using microarray approaches

  • Myung-Hwa, Jung;Jun-Young, Song;Sung-Ju, Jung
    • 한국어병학회지
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    • 제35권2호
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    • pp.141-155
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    • 2022
  • Rock bream iridovirus (RBIV) causes high mortality and economic losses in the rock bream (Oplegnathus fasciatus) aquaculture industry in Korea. Viral open reading frames (ORFs) expression profiling at different RBIV infection stages was investigated using microarray approaches. Rock bream were exposed to the virus and held for 7 days at 23 ℃ before the water temperature was reduced to 17 ℃. Herein, 28% mortality was observed from 24 to 35 days post infection (dpi), after which no mortality was observed until 70 dpi (end of the experiment). A total of 27 ORFs were significantly up- or down-regulated after RBIV infection. In RBIV-infected rock bream, four viral genes were expressed after 2 dpi. Most RBIV ORFs (26 genes, 96.2%) were significantly elevated between 7 and 20 dpi. Among them, 12 ORF (44.4%) transcripts reached their peak expression intensity at 15 dpi, and 14 ORFs (51.8%) were at peak expression intensity at 20 dpi. Expression levels began to decrease after 25 dpi, and 92.6% of ORFs (25 genes) were expressed below 1-fold at 70 dpi. From the microarray data, in addition to the viral infection, viral gene expression profiles were categorized into three infection stages, namely, early (2 dpi), middle (7 to 20 dpi), and recovery (25 and 70 dpi). RBIV ORFs 009R, 023R, 032L, 049L, and 056L were remarkably expressed during RBIV infection. Furthermore, six ORFs (001L, 013R, 052L, 053L, 058L, and 061L) were significantly expressed only at 20 dpi. To verify the cDNA microarray data, we performed quantitative real-time PCR, and the results were similar to that of the microarray. Our results provide novel observations on broader RBIV gene expression at different stages of infection and the development of control strategies against RBIV infection.

Induction of antiviral mechanisms by interferon-related genes in rock bream (Oplegnathus fasciatus) infected with rock bream iridovirus (RBIV)

  • Myung-Hwa Jung
    • 한국어병학회지
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    • 제36권2호
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    • pp.213-228
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    • 2023
  • We evaluated the transcriptional response of interferon (IFN)-related genes in rock bream iridovirus (RBIV)-infected rock bream under high-, low-, or no-mortality conditions induced by different stocking water temperatures. Under the high susceptibility condition (group A, water temperature 26℃, 100% mortality), only the Mx gene was expressed early, with prolonged expression, and with heavy viral loads of approximately 106~107 major capsid protein gene copies/μL from 4 to 10 days post infection (dpi). However, IRF1, IRF3, IRF8, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56 were activated at later time points (8 dpi) and then quickly decreased (10 dpi). For the low susceptibility condition, the water temperature was set at 23℃ for 7 days (group B) and then reduced to 17℃. Group B exhibited a 28% mortality rate, in which persistent and effective antiviral responses were observed for long periods of time. In particular, at 20 and 22 dpi, when virus replication was peaked at approximately 107/μL, the expressions of most of the IFN-related genes (IRF1, IRF3, IRF8, Mx, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56) were significantly higher in group B than in the control group. Moreover, prolonged and higher levels of IRF3 (at least 30 dpi), IRF8 (at least 30 dpi), ISG15 (at least 30 dpi), PKR (at least 28 dpi), Viperin (at least 30 dpi), and IFI44 (at least 30 dpi) were also observed in the recovery stage of infection. Under the no-susceptibility condition at 17℃ (0% mortality), significantly elevated levels of IRF3, Mx, ISG15, and PKR were observed mostly until 20 dpi. The findings indicate that RBIV infection can induce an efficient IFN-mediated antiviral immune response in low- and no-susceptibility conditions. The findings could be valuable for effective control of viral pathogens in fish.

2019년부터 2023년까지 국내에서 분리된 참돔이리도바이러스의 계통 분류 및 항원 결정기 예측 (Phylogenetic analysis and antigenic determinant prediction of red sea bream iridovirus isolated in Korea from 2019 to 2023)

  • 김국현;민준규;정현도;김광일
    • 한국어병학회지
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    • 제37권1호
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    • pp.25-36
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    • 2024
  • In this study, we analyzed the phylogenetic classification, epitope prediction, and pathogenicity of red sea bream iridovirus (RSIV) isolated from rock bream between 2019 and 2023. Phylogenetics based on genes encoding MCP and ATPase indicated that all five RSIV isolates belonged to RSIV subtype II. The deduced amino acid sequence of the MCP for the amplicons (1362 bp) obtained from RSIV isolates had a length of 453 amino acids. Among these, the amino acid sequences of the RSIV-19, 21, 22, and 23 isolates showed 100% identity, while the RSIV-20 isolate showed 99.78% identity with one residue difference at position 306. As a result of antigenicity analysis based on amino acid sequence, the antigenicity score of the RSIV-20 isolate was 0.6386 and the other RSIV isolates were 0.6365. Additionally, the prediction of their antigenic determinants resulted in a total of 17 identical antigenic plots. When each RSIV was inoculated into rock bream, no significant differences were observed with 100% cumulative mortality in all groups. This study provides data on the potential for genetic variation of RSIV isolated in the same marine area over the past five years, and the antigenicity and pathogenicity results of each isolate are expected to be useful information for selecting future vaccine strains.

Characterization of rock bream (Oplegnathus fasciatus) fin cells and its susceptibility to different genotypes of megalocytiviruses

  • Jeong, Ye Jin;Kim, Young Chul;Min, Joon Gyu;Jeong, Min A;Kim, Kwang Il
    • 한국어병학회지
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    • 제34권2호
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    • pp.149-159
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    • 2021
  • Genus Megalocytivirus cause red sea bream iridoviral disease (RSIVD) and scale drop disease (SDD). Based on the phylogeny of the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes, megalocytiviruses except for SDD virus (SDDV) could be three different genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis (ISKNV), and turbot reddish body iridovirus (TRBIV). In this study, primary cells derived from the caudal fin of rock bream (Oplegnathus fasciatus) grew at 25℃ in Leibovitz's medium supplemented with 10% (v/v) fetal bovine serum and primocin (100 ㎍/mL). Rock bream fin (RBF) cells exhibited susceptibility to infections by different genotypes of megalocytiviruses (RSIV, ISKNV and TRBIV) with the appearance of cytopathic effects with an increase in the viral genome copy number. Furthermore, compared to grunt fin (GF) cells, even though 10 times lower number of RSIV genome copies were inoculated in RBF cells, viral genome copy number produced on RBF cells were 44 times higher than that of GF cells at 7 d post-inoculation. As the isolated RBF cells are sensitive to different genotypes of megalocytiviruses (RSIV, ISKNV and TRBIV), they can be used for future studies regarding in vitro viral infection and subsequent diagnosis.