Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Soybeans Fermented with Bacillus po/yfermenticus KJS-2 Protects Oplegnathus fasciatus from Iridovirus and Pathogenic Bacterial Infection

Bacillus polyfermenticus KJS-2에서 발효된 콩의 돌돔에 대한 이리도바이러스 및 병원성균에 대한 예방효과

  • Published : 2009.06.30
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Abstract

Lyophilized powder (BP2FS) of soybeans fermented with Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) exhibited in vitro antibacterial activities against eight pathogenic bacteria. BP2FS was used as a fodder additive for Oplegnathus fasciatus (0. fasciatus) culture. One group (UFD) of O. fasciatus was fed a commercial fodder, while another group (FD) was fed the same fodder, but including BP2FS ($6{\times}10^{4}$ cfu $g^{-1}$ fodder), two times daily for 120 days. The mean body weight of the FD group (67.29${\pm}$12.62 g) was higher than that of the UFD group (56.56${\pm}$8.21 g) after 120 days. The survival rate of FD was 80% compared to 40% for the UFD group. Cumulative mortalities in the FD and UFD groups were 18.95% and 60.98% respectively. B. polyfermenticus KJS-2 was isolated from the intestines of the FD group and the number of viable colonies was estimated to be $1.04{\times}10^{4}$ cfu $g^{-1}$. Iridovirus and Vibrio vulnificus was detected in the organs of the UFD group but not in the FD group. All of the infected fish showed typical clinical symptoms of hemorrhage in their tail fins. Dissection of the infected internal organs revealed liver congestion and spleen enlargement - typical symptoms caused by iridovirus infection. These results clearly show that BP2FS is highly beneficial in preventing O. fasciatus from iridovirus infection.

Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2)를 이용하여 발효된 콩(BP2FS)은 8가지의 병원성균에 대해 항균활성을 가졌다. BP2FS는 돌돔의 배양에 있어 사료첨가제로 사용되었다. 돌돔의 배양시 BP2FS를 투여한 군($6{\times}10^{4}$ cfu $g^{-1}$ 사료)과 투여하지 않은 군으로 나누었으며, 투여한 군은 120일 동안 하루에 두 번씩 투여 하였다. 120일 후에 투여한 군의 돌돔의 몸무게는 67.29${\pm}$12.62 g이었으며 그렇지 않은 군의 몸무게는 56.56${\pm}$8.21 g으로 나타났다. 생존율은 투여한 군(80%)과 투여하지 않은 군(40%)이었다. 폐사한 돌돔의 축적된 양은 투여한 군(18.95%)과 투여하지 않은 군(60.98%)이었다. BP2FS를 투여한 군에서 B. polyfermenticus KJS-2를 돌돔의 장내에서 검출한 결과 $1.04{\times}10^{4}$ cfu $g^{-1}$으로 나타났다. BP2FS를 투여 하지 않은 군의 돌돔에서는 iridovirus에 감염되었을 경우 나타나는 전형적인 증상인 비장과 간에서 비대세포가 관찰 되었다. 이 결과들은 BP2FS는 돌돔의 이리도바이러스에 대한 감염 시 예방효과를 가져온다 할 수 있다.

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References

  1. Austin, B., L. F. Stuckey, P. A. W. Robertson, J. Effendi, and D. R. W. Griffith. 1995. A probiotic reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii. Journal of Fish Diseases 18, 93-96 https://doi.org/10.1111/j.1365-2761.1995.tb01271.x
  2. Andersson, A., P. E. Granum, and U. Ronner. 1998. The adhesion of Bacillus cereus spores to epithelial cells might be an additional virulence mechanism. International Journal of Food Microbiology 39, 93-99 https://doi.org/10.1016/S0168-1605(97)00121-9
  3. Blake, S., J. Y. Ma, D. A. Caporale, S. Jairath, and B. L. Nicholson. 2001. Phylogenetic relationships of aquatic birnaviruses based on deduced amino acid sequences of genome segment A cDNA. Diseases of Aquatic Organisms 45, 89-102 https://doi.org/10.3354/dao045089
  4. Cole, C. B. and R. Fuller. 1984. A note on the effect of host specific fermented milk on the coliform population of the neonatal rat gut. Journal of Applied Microbiology 56, 495-498 https://doi.org/10.1111/j.1365-2672.1984.tb01379.x
  5. Chauviere, G., M. H. Coconnier, S. Kerneis, Darfeuille- Michaud., B. Joly, and A. L. Servin. 1992. Competitive exclusion of diarrheagenic Escherichia coli (ETEC) from enterocyte-like Caco-2 cells in culture. FEMS Microbiology Letters 49, 213-218
  6. Douillet, P. A. and C. J. Langdon. 1994. Use of a probiotic for culture of larvae of the Pacific oyster (Crassostrea, gigas Thuberg). Aquaculture 119, 25-40 https://doi.org/10.1016/0044-8486(94)90441-3
  7. Do, J. W., C. H. Moon, H. J. Kim, M. S. Ko, S. B. Kim, J. H. Son, J. S. Kim, E. J. An, M. K. Kim, S. K. Lee, M. S. Han, S. J. Cha, M. S. Park, M. A. Park, Y. H. Kim, J. W. Kim, and J. W. Park. 2004. Complete genomic DNA sequence of rock bream iridovirus. Virology 325, 351-363 https://doi.org/10.1016/j.virol.2004.05.008
  8. Fuller, R. 1989. Probiotics in man and animals. Journal of Applied Bacteriology 66, 365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  9. Goren, E., W. A. De Jong, P. Doornenbal, J. P. Koopman, and H. M. Kennis. 1984. Protection of chicks against Salmonella infection induced by spray application of intestinal microflora in the hatchery. The Veterinary Quarterly 6, 73-79 https://doi.org/10.1080/01652176.1984.9693915
  10. Gram, L., J. Melchiorsen, B. Spanggaard, I. Huber, and T. F. Nielsen. 1999. Inhibition of Vibrio anguillarum by Pseudomonas fluorescens AH2, a Possible Probiotic Treatment of Fish. Applied and Environmental Microbiology 65, 969-973
  11. He, J. G., S. P. Wang, K. Zeng, Z. J. Huang, and S. M. Chan. 2002. Systemic disease caused by an iridovirus-like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China. Journal of Fish Diseases 23, 219-222 https://doi.org/10.1046/j.1365-2761.2000.00213.x
  12. Irianto, A. and B. Austin. 2002. Probiotics in aquaculture. Journal of Fish Diseases 25, 633-642 https://doi.org/10.1046/j.1365-2761.2002.00422.x
  13. Jung, S. J. and M. J. Oh. 2000. Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Koreanpeninsula. Journal of Fish Diseases 23, 223-226 https://doi.org/10.1046/j.1365-2761.2000.00212.x
  14. Kennedy, S. B., J. W. Tucker, C. L. Neidig, G. K. Vermeer, V. R. Cooper, J. L. Jarrell, and D. G. Sennett. 1998. Bacterial management strategies for stock enhancement of warm water marine fish: A case study with common snook Centropomus undecimalis. Bulletin of Marine Science 62, 573-588
  15. Kim, Y. J., S. J. Jung, T. J. Choi, H. R. Kim, K. V. Rajendran, and M. J. Oh. 2002. PCR amplification and sequence analysis of irido-like virus infecting fish in Korea. Journal of Fish Diseases 25, 121-124 https://doi.org/10.1046/j.1365-2761.2002.00345.x
  16. Kim, D. H., H. K. Oh, J. I. Eou, H. J. Seo, S. K. Kim, M. J. Oh, S. W. Nam, and T. J. Choi. 2005. Complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish. Virus Research 107, 1-9 https://doi.org/10.1016/j.virusres.2004.06.004
  17. Kim, K. M., J. Y. Lee, C. K. Kim, and J. S. Kang. 2009. Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2. Archives of Pharmacal Research 32, 711-715 https://doi.org/10.1007/s12272-009-1509-2
  18. Langdon, J. S., J. D. Humphrey, L. M. Williams, A. D. Hyatt, and H. A. Westbury. 1986. First virus isolation from Australian fish: an iridovirus-like pathogen from redfin perch, Perca fluviatilis L. Journal of Fish Diseases 9, 263-268 https://doi.org/10.1111/j.1365-2761.1986.tb01011.x
  19. Lai, Y. S., J. A. C. John, C. H. Lin, I. C. Guo, S. C. Chen, K. Fang, C. H. Lin, and C. Y. Chang. 2003. Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido- and nodaviruses. Journal of Fish Diseases 26, 31-42 https://doi.org/10.1046/j.1365-2761.2003.00434.x
  20. Lee, K. H., K. D. Jun, W. S. Kim, and H. D. Paik. 2001. Partial characterization of polyfermenticin SCD, a newly identified bacteriocin of Bacillus polyfermenticus. Letters in Applied Microbiology 32, 146-151 https://doi.org/10.1046/j.1472-765x.2001.00876.x
  21. Lilly, D. M. and R. H. Stillwell. 1965. Probiotics: Growth promoting factors produced by microorganisms. Science 147, 747-748 https://doi.org/10.1126/science.147.3659.747
  22. Nishizawa, T., K. I. Mori, M. Furuhashi, T. Nakai, I. Furusawa, and K. Muroga. 1995. Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. Journal of General Virology 76, 1563-1569 https://doi.org/10.1099/0022-1317-76-7-1563
  23. Oh, D. J., J. Y. Kim, J. A. Lee, W. J. Yoon, S. Y. Park, and Y. H. Jung. 2007. Complete mitochondrial genome of the rock bream Oplegnathus fasciatus (Perciformes, Oplegnathidae) with phylogenetic considerations. Gene 392, 174-180 https://doi.org/10.1016/j.gene.2006.12.007
  24. Oh, M. J., S. I. Kitamura, W. S. Kim, M. K. Park, S. J. Jung, T. Miyadai, and M. Ohtani. 2006. Susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, Psetta maximus (L.). Journal of Fish Diseases 29, 415-421 https://doi.org/10.1111/j.1365-2761.2006.00734.x
  25. Oshima, S., J. Hata, N. Hirasawa, T. Ohtaka, I. Hirono, T. Aoki, and S. Yamashita. 1998. Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction. Diseases of Aquatic Organisms 32, 87-90 https://doi.org/10.3354/dao032087
  26. Parker, R. B. 1976. Dental office procedures for breaking an infection chain. Journal of the American Society for Preventive Dentistry 6, 18-21
  27. Perera, R. P., S. K. Johnson, and D. H. Lewis. 1997. Epizootiological aspects of Streptococcus iniae affecting tilapia in Texas. Aquaculture 152, 25-33 https://doi.org/10.1016/S0044-8486(96)01450-0
  28. Rengpipat, S., W. Phianphak, S. Piyatiratitivorakul, and P. Menasveta. 1998. Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167, 301-313 https://doi.org/10.1016/S0044-8486(98)00305-6
  29. Rengpipat, S., S. Rukpratanporn, S. Piyatiratitivorakul, and P. Menasaveta. 2000. Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture 191, 271-288 https://doi.org/10.1016/S0044-8486(00)00440-3
  30. Schiitze, H., P. J. Enzmann, R. Kuchling, E. Mundt, H. Niemann, and T. C. Mettenleiter. 1995. Complete genomic sequence of the fish rhabdovirus infectious haematopoietic necrosis virus. Journal of General Virology 76, 2519-2527 https://doi.org/10.1099/0022-1317-76-10-2519
  31. Sugita, H., N. Mutsuo, K. Shibuya, and Y. Deguchi. 1996. Production of antibacterial substances by intestinal bacteria isolated from coastal crab and fish species. Journal of Marine Biotechnology 4, 220-223
  32. Verschuere, L., G. Rombaut, P. Sorgeloos, and W. Verstraete. 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews 64, 655-671 https://doi.org/10.1128/MMBR.64.4.655-671.2000
  33. Wang, C. S., H. H. Shih, C. C. Ku, and S. N. Chen. 2003. Studies on epizootic iridovirus infection among red sea bream, Pagrus major (Temminck & Schlegel), cultured in Taiwan. Journal of Fish Diseases 26, 127-133 https://doi.org/10.1046/j.1365-2761.2003.00441.x
  34. Weng, S. P., Y. Q. Wang, J. H. He, M. Deng, L. Lu, H. J. Guan, Y. J. Liu, and S. M. Chan. 2002. Outbreaks of an iridovirus in red drum, Sciaenops ocellata (L.), cultured in southern China. Journal of Fish Diseases 25, 681-685 https://doi.org/10.1046/j.1365-2761.2002.00419.x