• Title/Summary/Keyword: invertible

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Expression of an Antimicrobial Peptide Magainin by a Promoter Inversion System

  • Lee, Jae-Hyun;Hong, Seung-Suh;Kim, Sun-Chang
    • Journal of Microbiology and Biotechnology
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    • v.8 no.1
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    • pp.34-41
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    • 1998
  • A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

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Tunable Q-factor 2-D Discrete Wavelet Transformation Filter Design And Performance Analysis (Q인자 조절 가능 2차원 이산 웨이브렛 변환 필터의 설계와 성능분석)

  • Shin, Jonghong
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.11 no.1
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    • pp.171-182
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    • 2015
  • The general wavelet transform has profitable property in non-stationary signal analysis specially. The tunable Q-factor wavelet transform is a fully-discrete wavelet transform for which the Q-factor Q and the asymptotic redundancy r, of the transform are easily and independently specified. In particular, the specified parameters Q and r can be real-valued. Therefore, by tuning Q, the oscillatory behavior of the wavelet can be chosen to match the oscillatory behavior of the signal of interest, so as to enhance the sparsity of a sparse signal representation. The TQWT is well suited to fast algorithms for sparsity-based inverse problems because it is a Parseval frame, easily invertible, and can be efficiently implemented. The transform is based on a real valued scaling factor and is implemented using a perfect reconstruction over-sampled filter bank with real-valued sampling factors. The transform is parameterized by its Q-factor and its over-sampling rate, with modest over-sampling rates being sufficient for the analysis/synthesis functions to be well localized. This paper describes filter design of 2D discrete-time wavelet transform for which the Q-factor is easily specified. With the advantage of this transform, perfect reconstruction filter design and implementation for performance improvement are focused in this paper. Hence, the 2D transform can be tuned according to the oscillatory behavior of the image signal to which it is applied. Therefore, application for performance improvement in multimedia communication field was evaluated.

${H^1}({\Omega})$-NORM ERROR ANALYSIS UNDER NUMERICAL QUADRATURE RULES BY THE P-VERSION OF THE FINITE ELEMENT METHOD

  • Kim, Ik-Sung;Kim, Chang-Geun;Song, Man-Suk
    • Communications of the Korean Mathematical Society
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    • v.9 no.2
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    • pp.467-489
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    • 1994
  • Let $\Omega$ be a closed and bounded polygonal domain in R$^2$, or a closed line segment in R$^1$ with boundary $\Gamma$, such that there exists an invertible mapping T : $\Omega$ \longrightarrow $\Omega$ with the following correspondence: x$\in$$\Omega$ ↔ x = T(x) $\in$$\Omega$, (1.1) and (1.2) t $\in$ U$\sub$p/($\Omega$) ↔ t = to T$\^$-1/ $\in$ U$\sub$p/($\Omega$), where $\Omega$ denotes the corresponding reference elements I = [-1,1] and I ${\times}$ I in R$^1$ and R$^2$ respectively, (1.3) U$\sub$p/($\Omega$) = {t : t is a polynomial of degree $\leq$ p in each variable on $\Omega$}, and (1.4) U$\sub$p/($\Omega$) = {t : t = to T $\in$ U$\sub$p/($\Omega$)}.(omitted)

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Image Restoration Based on Inverse Filtering Order and Power Spectrum Density (역 필터 순서와 파워 스펙트럼 밀도에 기초한 이미지 복원)

  • Kim, Yong-Gil;Moon, Kyung-Il
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.16 no.2
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    • pp.113-122
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    • 2016
  • In this paper, we suggest a approach which comprises fast Fourier transform inversion by wavelet noise attenuation. It represents an inverse filtering by adopting a factor into the Wiener filtering, and the optimal factor is chosen to minimize the overall mean squared error. in order to apply the Wiener filter, we have to compute the power spectrum of original image from the corrupted figure. Since the Wiener filtering contains the inverse filtering process, it expands the noise when the blurring filter is not invertible. To remove the large noises, the best is to remove the noise using wavelet threshold. Wavelet noise attenuation steps are consisted of inverse filtering and noise reduction by Wavelet functions. experimental results have not outperformed the other methods over the overall restoration performance.

On the Public Key Cryptosystems over Imaginary Quadratic Fields (복소 이차체위에서의 공개키 암호계에 관한 소고)

  • Kim, Yong-Tae
    • The Journal of the Korea institute of electronic communication sciences
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    • v.4 no.4
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    • pp.270-273
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    • 2009
  • In 1988, Buchmann et al. proposed a public key cryptosystem making use of ideals of the maximal orders in quadra tic fields which may pave the way for a public key cryptosystem using imaginary quadratic non-invertible ideals as generators. Next year, H$\ddot{u}$hnlein, Tagaki et al. published the cryptosystem with trapdoor and conductor prime p over non-maximal orders. On the other hand Kim and Moon proposed a public key cryptosystrem and a key distribution cry ptotsystem over class semigroup in 2003. We, in this paper, introduce and analyze the cryptotsystems mentioned above.

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Non-rigid Image Registration using Constrained Optimization (Constrained 최적화 기법을 이용한 Non-rigid 영상 등록)

  • Kim Jeong tae
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.10C
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    • pp.1402-1413
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    • 2004
  • In non-rigid image registration, the Jacobian determinant of the estimated deformation should be positive everywhere since physical deformations are always invertible. We propose a constrained optimization technique at ensures the positiveness of Jacobian determinant for cubic B-spline based deformation. We derived sufficient conditions for positive Jacobian determinant by bounding the differences of consecutive coefficients. The parameter set that satisfies the conditions is convex; it is the intersection of simple half spaces. We solve the optimization problem using a gradient projection method with Dykstra's cyclic projection algorithm. Analytical results, simulations and experimental results with inhale/exhale CT images with comparison to other methods are presented.

The Improvement of Meshwarp Algorithm for Rotational Pose Transformation of a Front Facial Image (정면 얼굴 영상의 회전 포즈 변형을 위한 메쉬워프 알고리즘의 개선)

  • Kim, Young-Won;Phan, Hung The;Oh, Seung-Taek;Jun, Byung-Hwan
    • Proceedings of the Korea Information Processing Society Conference
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    • 2002.11a
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    • pp.425-428
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    • 2002
  • 본 논문에서는 한 장의 정면 얼굴 영상만으로 회전 변형을 수행할 수 있는 새로운 영상기반렌더링(Image Based Rendering, IBR) 기법을 제안한다. 3차원 기하학적 모델을 대신하면서 수평 회전 변형을 연출하기 위해, 특정 인물의 정면, 좌우 반측면, 좌우 측면의 얼굴 영상에 대한 표준 메쉬 집합을 작성한다. 변형하고자 하는 임의의 인물에 대해서는 정면 영상에 대한 메쉬만을 작성하고, 나머지 측면 참조 메쉬들은 표준 메쉬 집합에 의해 자동으로 생성된다. 입체적인 회전 효과를 연출하기 위해, 회전 변형시 발생할 수 있는 제어점들간의 중첩 및 역전을 허용하도록 기존의 두 단계 메쉬워프 알고리즘을 개선한 역전가능 메쉬워프 알고리즘(invertible meshwarp algorithm)을 제안한다. 이 알고리즘을 이용하여 다양한 남녀노소의 정면 얼굴 영상에 대해 회전에 따른 포즈 변형을 수행하여 비교적 자연스러운 포즈 변형 결과를 얻었다.

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Structural time-varying damage detection using synchrosqueezing wavelet transform

  • Liu, Jing-Liang;Wang, Zuo-Cai;Ren, Wei-Xin;Li, Xing-Xin
    • Smart Structures and Systems
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    • v.15 no.1
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    • pp.119-133
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    • 2015
  • This paper proposed a structural time-varying damage detection method by using synchrosqueezing wavelet transform. The instantaneous frequencies of a structure with time-varying damage are first extracted using the synchrosqueezing wavelet transform. Since the proposed synchrosqueezing wavelet transform is invertible, thus each individual component can be reconstructed and the modal participation factor ratio can be extracted based on the amplitude of the analytical signals of the reconstructed individual components. Then, the new time-varying damage index is defined based on the extracted instantaneous frequencies and modal participation factor ratio. Both free and forced vibrations of a classical Duffing nonlinear system and a simply supported beam structure with abrupt and linear time-varying damage are simulated. The proposed synchrosqueezing wavelet transform method can successfully extract the instantaneous frequencies of the damaged structures under free vibration or vibration due to earthquake excitation. The results also show that the defined time-varying damage index can effectively track structural time-varying damage.

Neogambogic acid relieves myocardial injury induced by sepsis via p38 MAPK/NF-κB pathway

  • Fu, Wei;Fang, Xiaowei;Wu, Lidong;Hu, Weijuan;Yang, Tao
    • The Korean Journal of Physiology and Pharmacology
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    • v.26 no.6
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    • pp.511-518
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    • 2022
  • Sepsis-associated myocardial injury, an invertible myocardial depression, is a common complication of sepsis. Neogambogic acid is an active compound in garcinia and exerts anthelmintic, anti-inflammatory, and detoxification properties. The role of neogambogic acid in sepsis-associated myocardial injury was assessed. Firstly, mice were pretreated with neogambogic acid and then subjected to lipopolysaccharide treatment to induce sepsis. Results showed that lipopolysaccharide treatment induced up-regulation of biomarkers involved in cardiac injury, including lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and troponin I (cTnI). However, pretreatment with neogambogic acid reduced levels of LDH, CK-MB, and cTnI, and ameliorated histopathological changes in the heart tissues of septic mice. Secondly, neogambogic acid also improved cardiac function in septic mice through reduction in left ventricular end-diastolic pressure, and enhancement of ejection fraction, fractional shortening, and left ventricular systolic mean pressure. Moreover, neogambogic acid suppressed cardiac apoptosis and inflammation in septic mice and reduced cardiac fibrosis. Lastly, protein expression of p-p38, p-JNK, and p-NF-κB in septic mice was decreased by neogambogic acid. In conclusion, neogambogic acid exerted anti-apoptotic, anti-fibrotic, and anti-inflammatory effects in septic mice through the inactivation of MAPK/NF-κB pathway.

Implementation of Levitation Controller for Toroidally-Wound Self-Bearing BLDC Motor Using Continuously Invertible Force Model (연속적 역변환이 가능한 힘 모델을 이용한 환형권선 셀프베어링 BLDC 모터의 부상 제어기 구현)

  • Choi, Won-Yeong;Choi, Jung-Kyu;Noh, Myounggyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.37 no.7
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    • pp.899-903
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    • 2013
  • A self-bearing is an electric machine that achieves both rotational actuation and magnetic levitation using a single magnetic structure. To be able to stably levitate the rotor in a self-bearing, one needs to have an inverse of the force-current model. However, the force-current model in a self-bearing motor is typically not square. Furthermore, the elements of the matrix vary with respect to the rotational angle, resulting in singularities of the pseudo-inverse at various angles. In this paper, we propose a new force-current model that eliminates the singularities by adding a constraint in coil currents. This constraint eliminates the flux density in the stator core so that the saturation problem in the previous study is avoided. By implementing this force-current model, we are able to implement a levitation controller for a toroidally-wound self-bearing BLDC motor. The model inversion and levitation are validated experimentally.