• 대한수의학회지
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• 제32권2호
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• pp.211-215
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• 1992

Fecal examination for survey of natural prevalence of Balantidium coli was performed on 1080 healthy bred pigs in Korea, and the positive rate was 56.2%. In order to observe if Balantidium coli might be a secondary invader after certain initiation of the intestinal lesions, the piglet groups preinfected with the protozoa experimentally were treated with Salmonella cholerasuis, Trichuris. cold stress, HCI, and immunosuppressive drug respectively, but no relation was found between them. Also the protozoa were not detected from the intestinal lesions spontaneously formed of 107 pigs which were checked in the slaughter house. According to the above results, Balantidium coli is not directly associated with the formation of any lesions in the pig's large intestine.

• 한국동물위생학회지
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• 제15권2호
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• pp.195-202
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• 1992

Fecal Examination for survey of natural prevalence of Balantidium coli was performed on 1,080 healthy bred pigs in Korea, and the positive rate was 56.2%. In order to observe if Balantidium coli might be a secondary invader after certain initiation of the Intestinal lesions, the piglet groups preinfected with the protozoa experimentally were treated with salmonella cholerasuis, Trichuris, cold stress, HCI, and immunosuppressive drug respectively, but no relatios was found between them. Also the protozoa were not deteceted from the intestinal lesions spontaneously formed of 107 pigs which were checked in the slaughter house. According to the above results, Balantidium coli is not directly associated with the formation of any lesions in the pig's intestine.

• Parasites, Hosts and Diseases
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• 제22권1호
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• pp.116-126
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• 1984

The present study was designed to elucidate the recent prevalence of the intestinal protozoa and to demonstrate the relationship on prevalence of the surveyed population with their academic career and living conditions. During the period from May to July 1981, total 2, 735 fecal specimens were collected from the inhabitants of 11 areas in Gyeong-gi Do(province) near by Seoul City, and 6 areas in Jeonra Bug Do, southwestern part of Korea. The samples were fixed with SAF solution and were examined by formalin-ether sedimentation technique. The results are summarised as follows: 1, Overall positive rate of intestinal protozoa was 11. l% : Gyeong-gi Do, 10.7%; Jeonra Bug Do, 11.7%. 2. Five species of intestinal protozoa were detected: Entamoeba histolytica 0.69% : Entamoeba cali 5.52%; Endolimax nana 2.71%, Giardia lamblia 2.23%; lodamoeba biitschlii 0.18%. 3. Highest positive rates among the examined areas were recognized: Incheon City of Gyeong-gi Do, 26.7%; Im-sil Gun(county) of Jeonra Bug Do, 19.9%. 4. By species, E. nana showed the highest prevalence rate (13.74%) in Incheon City, and E. histolytica (2.37%) and E. coli (9.95%) in Im-sil Gun. 5. Prevalence of E. histolytica in rural areas was higher than in urban areas. 6. No significant difference in the prevalence rate by sect and age was recognized, although the group under 10 years and over 60 years were lower than in other age groups. 7, The highest positive rate of E. histolytica was observed in pauper group and significant linearity of prevalence rate of E. histozytica by monthly income was observed. 8. The inhabitants who used conventional latrine showed higher prevalence, 11.5%, than the group of inhabitants who used modified privy. 9. Although no significant difference of positive rate was recognized by academic career, but the lowest prevalence rate was observed in college graduates. 10. Over half of the surveyed population, 64.8% has no experience on anthelmintic practice. Number of anthelmintic application resulted no difference in prevalence. In conclusion, prevalences of intestinal protozoa in Gyeong-gi Do and Jeonra Bug Do decreased remarkably in comparison with those of one decade before. However, economic level, academic career and anthelmintic administration seemed no essential reasons for such declination.

• Parasites, Hosts and Diseases
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• 제41권2호
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• pp.113-115
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• 2003

We carried out a small-scale survey to investigate the status of intestinal protozoa and helminthes infection of inhabitants in Roxas city, Mindoro, the Philippines. Total 301 stool samples were subjected to the formalin-ether concentration method for the detection of helminth ova and protozoan cysts. The overall positive rate was 64.5%, and that of male and female were 56.6% and 72.5%, respectively. The highest infected helminth was Ascaris lumbricoudes (51.2%), followed by Trichuris trichiura (27.6%), hookworm (8.0%) and Enterobius vermicularis (0.3%). The protozoa infection status revealed that Entamoeba coli was the most frequent (15.0%). lodoamoeba buetschlii and E. histolytica were found but few. The multiple infection more than two parasites was 29.6%, and double infection with A. lumbricoides and T. trichiura was most common. The intestinal helminth infections were highly prevalent in this area, according to this result, and we concluded that anthelminthic drugs should be given to inhabiiants, especially to children of 1 to 15 years-old.

• Parasites, Hosts and Diseases
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• 제48권4호
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• pp.297-301
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• 2010

Recently, emerging waterbome protozoa, such as microsporidia, Cyclospora, and Cryptosporidium, have become a challenge to human health worldwide. Rapid, simple, and economical detection methods for these major waterborne protozoa in environmental and clinical samples are necessary to control infection and improve public health. In the present study, we developed a multiplex PCR test that is able to detect all these 3 major waterborne protozoa at the same time. Detection limits of the multiplex PCR method ranged from $10^1$ to $10^2$ oocysts or spores. The primers for microsporidia or Cryptosporidium used in this study can detect both Enterocytozoon bieneusi and Encephalitozoon intestinalis, or both Cryptosporidium hominis and Cryptosporidium paNum, respectively. Restriction enzyme digestion of PCR products with BsaBI or BsiEI makes it possible to distinguish the 2 species of microsporidia or Cryptosporidium, respectively. This simple, rapid, and cost-effective multiplex PCR method will be useful for detecting outbreaks or sporadic cases of waterborne protozoa infections.

• 생명과학회지
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• 제5권3호
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• pp.126-136
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• 1995

The studies had been conducted to evaluate the grain processing effects for ruminants on starch digestion, body weight gain and feed efficiency since 1970. This research deals with experimental results on chemical structure, gelatinization, microbial starch digestion in rumen, intestinal starch digestion in rumen, roles of protozoa, intestinal starch digestion of bypass starch, limits to starch digestion in small intestine. The grain processing has different effects on digestion, weight gain and feed efficiency when different grain sources and contents is used, and the quality and quantity of roughage is different. The economical and efficient method of grain processing should be selected considering weight gain and feed efficiency enhancement than digestibility.

• Parasites, Hosts and Diseases
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• 제24권2호
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• pp.209-212
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• 1986

The present study was undertaken to evaluate the present status of intestinal parasitic infection in Seoul area, Korea. During the period from June 1985 to July 1986, a total of 5,251 stool samples were collected in Department of Clinical Pathology, Hanyang University Hospital and examined by formalin-ether sedimentation technique once for helminth ova and protozoan cysts. The results were summarized as follows: 1. The overall egg positive rate of intestinal helminthes was 2.53%; and 1.43% for Clonorchis sinensis, 0.7% for Trichuris trichiura, 0.13% for Metagonimus yokogawai, 0.06% for hookworm, and 0.02% for Ascaris lumbricoides, Diphyllobothrium latum and Hymenolepis nana, respectively. 2. The overall cyst positive rate of intestinal protozoa was 1.07%. Cyst positive rate was 0.06% for Entamoeba histolytica and 0.13% for Giardia lamblia, respectively. 3. Higher prevalence of intestinal parasitic infection was observed in male and in $21{\sim}50$ year-old groups. 4. The highest prevalence of Clonorchis sinensis infection was observed in 40th age group (3.4%), and male (2.3%) was more infected than female (0.7%) in general.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6175-6179
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• 2012

Intestinal parasitic infections (IPIs) are still important to the health of Thai rural residents. IPIs are the cause of many chronic diseases with, for example, opisthorchiasis resulting in progression to cholangiocarcinoma (CCA). This cross-sectional study in a prospective cohort study aimed to examine the prevalence and co-infection of intestinal parasites among Northeastern Thai rural residents, recruited into the Khon Kaen Cohort Study (KKCS), and who were residing in areas of high-risk for developing CCA. On recruitment, subjects had completed questionnaires and provided fecal samples for IPI testing using the formalin ethyl acetate concentration technique. Data on selected general characteristics and the results of the fecal tests were analysed. IPI test results were available for 18,900 of cohort subjects, and 38.50% were found to be positive for one or more types of intestinal parasite. The prevalence of Opisthorchis viverrini (O. viverrini) infection was the highest (45.7%), followed by intestinal flukes (31.9%), intestinal nematodes (17.7%), intestinal protozoa (3.02%), and intestinal cestodes (1.69%). The pattern of different infections was similar in all age groups. According to a mapping analysis, a higher CCA burden was correlated with a higher prevalence of O. viverrini and intestinal flukes and a greater intensity of O. viverrini. Both prevention and control programs against liver fluke and other intestinal parasites are needed and should be delivered simultaneously. We can anticipate that the design of future control and prevention programmes will accommodate a more community-orientated and participatory approach.

• Parasites, Hosts and Diseases
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• 제26권4호
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• pp.275-284
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• 1988

1988년 2월 및 5월에 전라남도 여천군 남면 소재 연도 주민 1,011명 (남자 507명, 여자 504명)에 대하여 formalin-ether 침전법으로 장내 기생충 감염상환을 조사하고, 장내 윤충란 양성자 의 경우 감염강도를 E.P.G.로 측정한 바 다음과 같은 결과를 얻었다. 주민 1,011명중 398명 (39.4%)이 장내 기생충 양성자이었고 윤충 감염이 372명 (36.8%), 원충 감 염이 56명(5.5%)이었다. 검출된 기생충은 모두 10종으로서 편충란 양성자가 278명(27.5%)으로 가 장 많았고 그 다음이 회충 176명(17.4U, 불수정란 양성자 31명 포함), Taenia sp. 59명(5.8%)이었으며, 대장아메바 3.3%, 람블편모충 1.5%, 왜소아메바 0.8%, 왜소조충 0.4%이었고, 구충, 동 양모양선충 및 이질아메바가 각각 0.2%의 양성률을 보였다. 성별 감염률은 여자가 44.0%로 전 연령군에서 남자 34.7%보다 높았다. 연령별로는 40대가 46.0%로 가장 높았고 10세 미만이 33.1%로 가장 낮았다. 회충란 양성자중 149명의 E.P.G.는 평균 1,876(범위 200∼17,800), 편충 E.P.G. 는 검사자 242명에서 평균 327(범위 200∼1,600)이었다. 장내 기생 윤충의 양성자 572명중 단일 감염은 236명 (63.4%), 2종 감염은 129명 (34.7%), 3종 감염은 7명 (1.9%)이었고, 원충시스트 양성자 56명 중 단일 감염은 53명 (94.6%), 2종 감염은 3명 (5.4%)이었다. 이상의 결과로 보아 낙도 주민의 장내 기생충 감염률은 높음을 알 수 있으며 이의 퇴치를 위하여 생환 주변 환경의 개선, 집단 치료, 보건 교육 등 적극적인 관리가 시급히 수행되어야 할 것으로 생각된다.

• Parasites, Hosts and Diseases
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• 제58권5호
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• pp.583-587
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• 2020

Blastocystis sp. is a kind of protozoa living in the intestinal tract of human and animals, which will cause intestinal diseases such as diarrhea, abdominal distension and vomiting. This paper was aimed to understand the infection of Blastocystis sp. In golden monkeys and the transmission path in North China. Thirty-seven feces samples from golden monkeys and 116 cockroach samples from Shijiazhuang Zoo were collected from July to October 2019 for PCR analysis of Blastocystis sp. Genetic diversity analysis was further conducted on the samples with positive PCR results. The results showed that the infection rate was 48.7% (18/37) in golden monkeys and 82.8% (96/116) in cockroaches, respectively. The genetic evolution analysis based on small subunit ribosomal RNA demonstrated that three subtypes (ST) of Blastocystis sp. including ST1, ST2, and ST3 existed in the intestinal tract of golden monkeys, while only ST2 was detected in the intestinal tract of cockroaches. This paper may provide supports for the quarantine and control of Blastocystis sp. for the zoo in Northern China.