• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• Journal of Chest Surgery
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• v.38 no.12 s.257
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• pp.856-859
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• 2005

A male neonate was referred to our hospital with facial cyanosis and tachypnea at 19 days of age. Two-dimensional echocardiography showed type B interrupted aortic arch, posterior malalignment ventricular septal defect and valvular aortic stenosis. A new surgical repair was done with biventricular repair and neo-aortic arch reconstruction. Left ventricular outflow track (LVOT) was consisted of aortic valve and pulmonic valve. Right ventricular outflow (RVOT) track was reconstructed with extracardiac conduit. Postoperative two-dimensional echocardiography showed no stenosis and turbulency flow on LVOT and RVOT.

• Journal of Chest Surgery
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• v.38 no.12 s.257
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• pp.852-855
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• 2005

A 85-day-old infant was successfully operated on for truncus arteriosus (type I) with interrupted aortic arch (type A) using one-stage anterior approach without circulatory arrest. Aortic arch was reconstructed by direct anastomosis of ascending aorta and descending aorta with regional perfusion and continuity of right ventricle to pulmonary artery was established with $Shelhigh^{circledR}$ pulmonic conduit. The patient experienced left bronchus compression by descending aorta immediately postoperatively, which was improved with positional change and physiotherapy. The patient had reoperation due to stenosis of valved conduit at 13 months later. The patient is currently well under follow-up of 14 months from initial repair.

• Journal of Korean Society of Transportation
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• v.26 no.1
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• pp.191-201
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• 2008

It has been employed TRANSYT-7F and NETSIM to evaluate the validity and effectiveness of improvement on TSM(Transportation Systems Management). But T7F is hard to describe platoon compression and dispersion in actually, and NETSIM takes a long time for network coding, calibration and have difficulty in setting up saturation flow. While Shockwave Model have advantage which can describe platoon compression and dispersion in actually and shorten hours, convenience of application. But Shockwave Model apply unrealistic traffic flow relation ship(U-K curve) and simplify platoon because of difficulty in calculating shockwave's position and cross. For solving limitation of existing shockwave models, It develop new model with 2-regime linear model, New platoon model, Extended shockwave, etc. For verifying the validity of the proposed model, it was compared with delay of T7F and NETSIM by offset variation. In conclusion, it is thought that proposed model have outstanding performance to simulate traffic phenomenon.

• Journal of Korean Society of Transportation
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• v.27 no.2
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• pp.133-144
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• 2009

In Korea, the ITS project has been progressed to improve traffic mobility and safety. Further, it is to relieve traffic jam by supply real time travel information for drivers and to promote traffic convenience and safety. It is important that the traffic information is provided accurately. This study was conducted outlier elimination and missing data adjustment to improve accuracy of raw data. A method for raise reliability of travel time prediction information was presented. We developed Historical Profile model and adjustment formula to reflect quality of interrupted flow. We predicted travel time by developed Historical Profile model and adjustment formula and verified by comparison between developed model and existing model such as Neural Network model and Kalman Filter model. The results of comparative analysis clarified that developed model and Karlman Filter model similarity predicted in general situation but developed model was more accurate than other models in incident situation.

• Health Policy and Management
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• v.28 no.2
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• pp.186-193
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• 2018

Background: The aim of our study was to review the findings of health insurance fraud investigations and to evaluate their impacts on medical costs for target and non-target organizations. An interrupted time series study design using generalized estimation equations was used to evaluate changes in cost following fraud investigations. Methods: We used National Health Insurance claims data from 2009 to 2015, which included 20,625 medical institutions (1,614 target organizations and 19,011 non-target organizations). Outcome variable included cost change after fraud investigation. Results: Following the initiation of fraud investigations, we found statistically significant reductions in cost level for target organizations (-1.40%, p<0.001). In addition, a reduction in cost trend change per month was found for both target organizations and non-target organizations after fraud investigation (target organizations, -0.33%; non-target organizations of same region, -0.19%; non-target organizations of other regions, -0.17%). Conclusion: This study suggested that fraud investigations are associated with cost reduction in target organization. We also found similar effects of fraud investigations on health expenditure for non-target organizations located in the same region and in different regions. Our finding suggests that fraud investigations are important in controlling the growth of health expenditure. To maximize the effects of fraud investigation on the growth of health expenditure, more organizations needed to be considered as target organizations.

• Journal of environmental and Sanitary engineering
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• v.24 no.4
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• pp.112-115
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• 2009

This research was performed to purify water quality through removing T-N, and T-P and to estimating rate of $NO_x$ by the response of photocatalyst using ceramics. The ceramics was a mixtures of Titanium Diboride($TiB_2$) which is used to develop armored cars with excellent protective power and lightness, Olivine, and Hwangto with water and was furnaced for an hour at $1160^{\circ}C$. Hwangto and Olivine used in the study are produced at Haenam-gun, Jeonnam, and Andong-city, Gyeongsangbuk-do, respectively. The ground Hwangto and Olivine were seived through PR $100{\times}200$, and $TiB_2$ was a product of SIGMA ALDRICH. The experiment was performed under the sunlight, Mass flow controller was used for constant flow to pass through the pyrex reactor which was fully charged with furnaced ceramics. The concentration of $NO_2$ gas passed through the pyrex reactor was measured by Multi Gas Monitor. The reaction took for 60 minutes, The material was exposed to sunlight for 40 minutes. The sunlight was interrupted before and after the exposure for 10 minutes. The result showed that the concentration of $NO_2$ gas of the ceramics with $TiB_2$ and without $TiB_2$ became reduced and then maintained the same concentration under the sunlight, and became increased when the sunlight is interrupted. The $NO_2$ removing efficiency of the ceramics mixed with $TiB_2$ was 14~43% higher than that of the ceramics without $TiB_2$ under the sunlight. The study showed that the removing rate of $NO_2$ was 80% when the mixture rate of Hwangto, Olivine, and $TiB_2$ was 68%, 30% and 2%.

• Journal of Chest Surgery
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• v.45 no.1
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• pp.19-23
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• 2012

Background: A ring implantation in the tricuspid annulus requires many interrupted mattress sutures for correction of tricuspid regurgitation (TR). In this study, tricuspid ring annuloplasty was performed by 2-0 polypropylene continuous suture instead of multiple interrupted 2-0 polyester mattress sutures, and the efficacy of the method was evaluated. Materials and Methods: This study included 20 patients who underwent tricuspid ring annuloplasty by continuous suture between May 2009 and July 2010. Four of the patients had an isolated TR, and the rest had a left-sided cardiac lesion. The concomitant tricuspid annuloplasty was performed after the left-sided heart surgery was completed and a Duran flexible ring prosthesis was used. Results: There was no perioperative mortality or conduction problem. More than a moderate degree of TR was improved to less than a mild degree after the procedure. After the ring annuloplasty, the right atrial volume decreased from $123.7{\pm}69.2mL$ to $74.5{\pm}37.4mL$, and the mean right atrial pressure was lowered from $18.7{\pm}12.2mmHg$ to $8.9{\pm}5.5mmHg$. Conclusion: The continuous "over and over" suture may be a useful procedure for fixing the ring to the annulus and making an intentional annular placation in performing tricuspid ring annuloplasty.

• Journal of Korean Society of Transportation
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• v.18 no.5
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• pp.57-67
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• 2000

Two fuzzy travel time estimators for interrupted traffic flow were developed based on field survey data and simulation data 7hat is collected from DETSIM, which is microscopic traffic simulation model that car-following theory is applied. One is FETTOS(Fuzzy Estimator of Travel Time using Occupancy and Spot speed) and the other is FETTOS(Fuzzy Estimator of Travel Speed using Volume and Occupancy). Fuzzy logic controller was applied to the estimators to deal with non-linear relationship between traffic variables and travel time. According to results of simulation and field survey. estimation of travel time can be modeled by using percent occupancy better than any other traffic variables. Detector location from storyline and signal timing Plan of intersection are affected to estimate travel time. With a few findings, the estimator was constructed and its performance was tested for observed travel time data and simulated data. FETTOS which needs signal timing plan and detector location estimates travel time with accurate better than FETSVO does. However. FETSVO has excellent transferability because the estimator needs set of input data only; volume and time mean speed.

• Journal of Korean Society of Transportation
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• v.20 no.4
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• pp.19-26
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• 2002

The purpose of this paper is to study the fundamental characteristics of bicycle flows. Several experiments were conducted to obtain the characteristics of bicycle flows, speed variation along a curve radius, bicycle driver's travelling territory and saturation flow rate at signalized intersection. Bicycle facilities are categorized into uninterrupted and interrupted, the capacity of uninterrupted is approximately 5000bic/h, and that of the interrupted (at signalized intersection) is approximately 3000bic/h, when a curve radius is over 20m, bicycle speed is not increasing. Bicycle driver's travelling territory is used to occupancy area, it is the same concept as pedestrian's. Bicycle occupancy area is to be divided into circulation zone, comfort zone. and collision zone. Circulation zone is over 2.21$\times$4.1m and collision zone is less than 0.96$\times$2.47m. Comfort zone is defined as intermediate state between two zones.