• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.6
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• pp.510-515
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• 2006

The putative EPA synthesis gene cluster was mined from the entire genome sequence of Shewanella oneidensis MR-1. The gene cluster encodes a PKS-like pathway that consists of six open reading frames (ORFs): ORFSO1602 (multi-domain beta-ketoacyl synthase, KS-MAT-4ACPs-KR), ORFSO1600 (acyl transferase, AT), ORFSO1599 (multi-domain beta-ketoacyl synthase, KS-CLF-DH-DH), ORFSO1597 (enoyl reductase, ER), ORFSO1604 (phosphopentetheine transferase, PPT), and ORFSO1603 (transcriptional regulator). In order to prove involvement of the PKS-like machinery in EPA synthesis, a 20.195-kb DNA fragment containing the genes was amplified from S. oneidensis MR-1 by the long-PCR method. Its identity was confirmed by the methods of restriction enzyme site mapping and nested PCR of internal genes orfSO1597 and orfSO1604. The DNA fragment was cloned into Escherichia coli using cosmid vector SuperCos1 to form pCosEPA. Synthesis of EPA was observed in four E. coli clones harboring pCosEPA, of which the maximum yield was 0.689% of the total fatty acids in a clone designated 9704-23. The production yield of EPA in the E. coli clone was affected by cultivation temperature, showing maximum yield at $20^{\circ}C$ and no production at $30^{\circ}C$ or higher. In addition, production yield was inversely proportional to glucose concentration of the cultivation medium. From the above results, it was concluded that the PKS-like modules catalyze the synthesis of EPA. The synthetic process appears to be subject to regulatory mechanisms triggered by various environmental factors. This most likely occurs via the control of gene expression, protein stability, or enzyme activity.

• Journal of the Korean Geosynthetics Society
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• v.5 no.4
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• pp.35-42
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• 2006

This paper describes a field experience on geogrid-reinforced soil walls rising up to 29.5m in height. Since experiences of design and construction on very high-raised geogrid reinforced soil wall were limited, thorough design and construction management was performed for safe construction of the wall. Regarding design of the wall, both internal and external stabilities were examined based on the design guideline specified by FHWA and overall slope stability analyses were performed by using Bishop simplified method. Moreover, a series of instrumentations were performed. The results of instrumentation for two tiered reinforced soil wall showed that not only the deformations of both the wall face and the reinforcement but also the horizontal earth pressures acting on the wall facing were very small. These results indicate that the reinforced soil wall technology can be applied successfully for high-raised tiered wall more than 20m heights and FHWA design guideline is very conservative for that large wall.

• Industrial Engineering and Management Systems
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• v.4 no.1
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• pp.47-53
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• 2005

The efficiency of fabrication (fab) operation is one of the key factors in order for a semiconductor manufacturing company to stay competitive. Optimization of manpower and forecasting manpower needs in a modern fab is an essential part of the future strategic planing and a very important to the operational efficiency. As the semiconductor manufacturing technology has entered the 8-inch wafer era, the complexity of fab operation increases with the increase of wafer size. The wafer handling method has evolved from manual mode in 6-inch wafer fab to semi-automated or fully automated factory in 8-inch and 12-inch wafer fab. The distribution of manpower requirement in each specialty varied as the trend of fab operation goes for downsizing manpower with automation and outsourcing maintenance work. This paper is to study the specialty distribution of manpower from the requirement in a typical 6-inch, 8-inch to 12-inch wafer fab. The human resource planning in today’s fab operation shall consider many factors, which include the stability of technical talents. This empirical study mainly focuses on the human resource planning, the manpower distribution of specialty structure and the forecast model of internal demand/supply in current semiconductor manufacturing company. Considering the market fluctuation with the demand of varied products and the advance in process technology, the study is to design a headcount forecast model based on current manpower planning for direct labour (DL) and indirect labour (IDL) in Taiwan’s fab. The model can be used to forecast the future manpower requirement on each specialty for the strategic planning of human resource to serve the development of the industry.

• Journal of the Institute of Convergence Signal Processing
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• v.1 no.2
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• pp.169-176
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• 2000

Deadbeat property is well established in digital control system design in time domain. But in continuous time system, deadbeat is impossible because of it's ripples between sampling points inspite of designs using the related digital control system design theory. But several researchers suggested delay elements. A delay element is made from the concept of finite Laplace Transform. From some specifications such as internal model stability, physical realizations as well as finite time settling, unknown coefficents and poles in error transfer functions with delay elements can be calulted so as to satisfy these specifications. For the application to the real system, robustness property can be added. In this paper, error transfer function is specified with 1 delay element and robustness condition is considered additionally. As the criterion of the robustness, a weighted sensitive function's $H_{infty}$ norm is used. For the minimum value of the criterion, error transfer function's poles are calculated optimally. In this sense, optimal design of the continuous time deadbeat controller is obtained.

• The Plant Pathology Journal
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• v.36 no.6
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• pp.515-535
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• 2020

Essential oils (EOs) have gained a renewed interest in many disciplines such as plant disease control and medicine. This review discusses the components of ginger EOs, their mode of action, and their potential nanotechnology applications in controlling tropical plant diseases. Gas chromatography-mass spectroscopy (GC-MS), high-performance liquid chromatography, and headspace procedures are commonly used to detect and profile their chemical compositions EOs in ginger. The ginger EOs are composed of monoterpenes (transcaryophyllene, camphene, geranial, eucalyptol, and neral) and sesquiterpene hydrocarbons (α-zingiberene, ar-curcumene, β-bisabolene, and β-sesquiphellandrene). GC-MS analysis of the EOs revealed many compounds but few compounds were revealed using the headspace approach. The EOs have a wide range of activities against many phytopathogens. EOs mode of action affects both the pathogen cell's external envelope and internal structures. The problems associated with solubility and stability of EOs had prompted the use nanotechnology such as nanoemulsions. The use of nanoemulsion to increase efficiency and supply of EOs to control plant diseases control was discussed in this present paper. The findings of this review paper may accelerate the effective use of ginger EOs in controlling tropical plant diseases.

• Journal of Chest Surgery
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• v.49 no.1
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• pp.1-8
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• 2016

More than a century ago, the first scientific report was published about fracture fixation with plates. During the 1950's, open reduction and plate fixation for fractures were standardized by the founders of Arbeitsgemeinschaft $f{\ddot{u}}r$ osteosynthesefragen/Association for the Study of Internal Fixation. Since the introduction of plate fixation for fractures, several plates and screws have been developed, all with their own characteristics. To accomplice more fracture stability, it was thought the bigger the plate, the better. The counter side was a compromised blood supply of the bone, often resulting in bone necrosis and ultimately delayed or non-union. With the search and development of new materials and techniques for fracture fixation, less invasive procedures have become increasingly popular. This resulted in the minimally invasive plate osteosynthesis (MIPO) technique for fracture fixation. With the MIPO technique, procedures could be performed with smaller incisions and thus with less soft tissue damage and a better preserved blood supply. The last 5 years rib fixation has become increasingly popular, rising evidence has becomeavailable suggesting that surgical rib fixation improves outcome of patients with a flail chest or isolated rib fractures. Many surgical approaches for rib fixation have been described in the old literature, however, most of these techniques are obscure nowadays. Currently mostly large incisions with considerable surgical insult are used to stabilize rib fractures. We think that MIPO deserves a place in the surgical treatment of rib fractures. We present the aspects of diagnosis, preoperative planning and operative techniques in regard to MIPO rib fixation.

• Tribology and Lubricants
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• v.31 no.6
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• pp.302-307
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• 2015

The use of turbocharger in internal combustion engines has increased as it is a key components for improving system efficiency without increasing engine size. Because of increasing demand, many studies have evaluated rotordynamic performance so as to increase rotation speed. This paper presents a linear and nonlinear analysis model for a turbocharger rotor supported by a floating ring bearing. We constructed rotor model by using the finite element method and approximated bearings as being infinitely short. In the linear model, we considered fluid film force as stiffness and damping element. In nonlinear analysis, calculation of the fluid film force involved solving the time dependent Reynolds equation. We verified the developed model by comparing the results to those of previous research. The analysis results show that there are four unstable modes, which are rigid body modes combining ring and rotor motion. As the rotating speed increases, the logarithmic decrement shows that certain unstable modes goes into the stable area or the stable mode goes into the unstable area. These unstable modes appear as sub-synchronous vibrations in nonlinear analysis. In nonlinear analysis frequency jump phenomenon demonstrated in several experimental studies appears. The analysis results also showed that frequency jump phenomenon occurs when the vibration mode changes and the sequence of unstable mode matches the linear analysis result. However, the natural frequency predicted using linear analysis differs from those obtained using nonlinear analysis.

• Journal of Korea Water Resources Association
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• v.39 no.1 s.162
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• pp.23-33
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• 2006

In this study, application potential of SINMAP, a GIS-based landslide hazard mapping tool, is evaluated through a case study. Through the application to the severe landslide events occurred during a heavy storm in 1991 on the Mt. Dalbong area about 78 kilometers south from Seoul, SINMAP successfully spotted most landslide sites. The effects and proper ranges of three calibration parameters of SINMAP, i.e. the soil internal friction angle, the combined cohesion of tree roots and soil, and T/R, were examined through comparison of predicted landslides with the landslide inventory data. From the findings of this study, it seems that SINMAP could be used as an effective screening tool for landslide hazard mapping especially for mountain areas with fairly steep slopes and relatively thin soil layers.

• Journal of Life Science
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• v.23 no.11
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• pp.1311-1316
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• 2013

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a major pre-mRNA binding protein and it is an abundant nuclear protein that shuttles between the nucleus and the cytoplasm. hnRNP L is known to be related to many cellular processes, including chromatin modification, pre-mRNA splicing, mRNA export of intronless genes, internal ribosomal entry site (IRES)-mediated translation, mRNA stability, and spermatogenesis. In order to identify the cellular proteins interacting with hnRNP L, this study performed a yeast two-hybrid screening, using a human liver cDNA library. The study identified insulin-like growth factor binding protein-4 (IGFBP-4) as a novel interaction partner of hnRNP L in the human liver. It then discovered, for the first time, that hnRNP L interacts specifically with IGFBP-4 in a yeast two-hybrid system. The authenticity of this two-hybrid interaction of hnRNP L and IGFBP-4 was confirmed by an in vitro pull-down assay.

• Journal of the Korean Geotechnical Society
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• v.34 no.2
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• pp.5-17
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• 2018

In this study, the in-situ stress, strength and stress-strain characteristics with shear parameters (UU, CU, ${\bar{CU}}$) are analytically evaluated and the stability analyses are carried out under loading/unloading conditions. The in-situ stress and the stress-strain behaviour may become different according to input shear parameters in finite element analyses with construction step, Especially, if the internal friction angle in Mohr-Coulomb model is set to zero, the in-situ stress and the stress-strain behaviour might not be properly predicted. The results from CU parameter of total stress analysis have no significant difference with the results from CU of effective stress analysis. Therefore, in the numerical analysis for soft ground, CU parameters can be applied to predict in-situ stress and stress-strain behaviors. In addition, the calculation method was proposed to determine the shear parameter of Mohr-Coulomb model, which is corresponding to the shear strength equivalent to that of in-situ soil.