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http://dx.doi.org/10.5352/JLS.2013.23.11.1311

Identification of the Interaction between Insulin-like Growth Factor Binding Protein-4 (IGFBP-4) and Heterogeneous Nuclear Ribonucleoprotein L (hnRNP L)  

Choi, Mieyoung (Department of Biomedical Sciences, Sunmoon University)
Publication Information
Journal of Life Science / v.23, no.11, 2013 , pp. 1311-1316 More about this Journal
Abstract
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a major pre-mRNA binding protein and it is an abundant nuclear protein that shuttles between the nucleus and the cytoplasm. hnRNP L is known to be related to many cellular processes, including chromatin modification, pre-mRNA splicing, mRNA export of intronless genes, internal ribosomal entry site (IRES)-mediated translation, mRNA stability, and spermatogenesis. In order to identify the cellular proteins interacting with hnRNP L, this study performed a yeast two-hybrid screening, using a human liver cDNA library. The study identified insulin-like growth factor binding protein-4 (IGFBP-4) as a novel interaction partner of hnRNP L in the human liver. It then discovered, for the first time, that hnRNP L interacts specifically with IGFBP-4 in a yeast two-hybrid system. The authenticity of this two-hybrid interaction of hnRNP L and IGFBP-4 was confirmed by an in vitro pull-down assay.
Keywords
Heterogeneous nuclear ribonucleoproteins (hnRNPs); hnRNP L; yeast two-hybrid system; Insulin-like growth factor binding protein-4 (IGFBP-4); GST pull-down assay;
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