• Journal of the Korean institute of surface engineering
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• v.42 no.4
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• pp.179-185
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• 2009

Diamond-like carbon(DLC) films were deposited by linear ion source(LIS)-physical vapor deposition method changing the anode voltages from 800 V to 1800 V, and characteristics of the films were investigated using residual stress tester, nano-indentation, micro raman spectroscopy, scratch tester and Field Emission Scanning Electron Microscope(FE-SEM). The results showed that the residual stress and hardness increased with increasing the ion energy up to anode voltage of 1400 V. It was also found that the content of $SP^3$ carbon increased with increasing the anode voltage $SP^3/SP^2$ ratio through investigation of $SP^3/SP^2$ ratio by the micro-raman analysis. From these results, it can be concluded that the physical properties of DLC films such as residual stress and hardness are increased with increasing the anode voltage. These results can be explained that 3-dimensional cross-links between carbon atoms and Dangling bond are enhanced and the internal compressive stress also increased with increasing the anode voltage. The optimal anode voltage is considered to be around 1400 V in these experimental conditions.

• Journal of the Korea institute for structural maintenance and inspection
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• v.14 no.2
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• pp.121-127
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• 2010

An experimental study of composite beam with perforated fiber reinforced polymer(FRP) plank as a permanent formwork and the tensile reinforcement was performed. A combined formwork and reinforcement system can facilitate rapid construction of concrete members since no conventional formwork is needed, which requires time consuming assembly and dismantling. In order for a smooth FRP plank to act compositely with the concrete, the surface of the FRP needs to be treated to increase its bond properties. Aggregates were bonded to the FRP plank using a commercially available epoxy and perforated web of plank. No additional flexural or shear reinforcement was provided in the beams. For comparison, two control specimens were tested. One control had no perforated hole in the web of FRP plank and the other had internal steel reinforcing bars instead of the FRP plank. The beams were loaded by central patch load to their ultimate capacity. This study demonstrates that the perforated FRP plank has the potential to serve as a permanent formwork and reinforcing for concrete beam.

• Journal of the Korean Magnetic Resonance Society
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• v.8 no.2
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• pp.77-85
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• 2004

$^1$H-detected $^{15}$N NMR was employed to investigated the effect of mutation (Y14F) on the dynamic properties of catalytic residues in ${\Delta}^5$-3- ketosteroid isomerase (KSI) from Conamonas testosteroni. In particular, the backbone dynamics of the catalytic residues have been studied in free enzyme and its complex with a steroid ligand, 19-nortestosterone hemisuccinate, by $^{15}$N relaxation measurements. The relaxation data were analyzed using the model-free formalism to extract the model-free parameters (S$^2$, ${\tau}_e$, and R$_{ex}$). The results show that the mutation causes a significant decrease in the order parameter (S$^2$) for the catalytic residues of free Y14F KSI, presumably due to breakdown of the hydrogen bond network by mutation. In addition, the order parameters of Phe-14 and Asp-99 increased slightly upon ligand binding, indicating a slight restriction of the high-frequency (pico- to nanosecond) internal motions of the residues in the complexed Y14F KSI, while the order parameter of Tyr-55 decreased significantly upon ligand binding.

• Journal of the Korean Wood Science and Technology
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• v.21 no.2
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• pp.57-65
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• 1993

The aim of this research was to manufacture sludge-particle board using paper sludge with wood particle and to investigate physical and mechanical properties of various sludge-particle boards, fabricated with ratios of sludge to particle of 10 to 90, 20 to 80, 30 to 70, 40 to 60 and 50 to 50(oven dry weight based). Sludge-particle boards were manufactured by urea-formaldehyde resin, 0.8 target specific gravity, and 10mm thickness. It was possible to manufacture sludge-particle board as the same processing in the present particleboard manufacturing system. This sludge-particle board have different properties as composition ratios of sludge and particle. And sludge-particle board made from 10 percent to 20 percent of sludge mixing ratio have similar mechanical properties compared with control particleboard. Especially, the sludge-particle board made from 10 percent to 40 percent mixing ratios of sludge have superior to control particleboard in internal bond, screw withdrawal holding strength and modulus of elasticity. In the case of dimensional stability, water absorption was increased and thickness swelling was decreased as increased with sludge mixing proportion. The sludge-particle board made of different mixing ratios of our laboratory design was able to concluded that there is possibility of partial substitution of wood particle materials.

• Journal of the Korean Chemical Society
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• v.36 no.4
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• pp.523-535
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• 1992

The conformations of hexapeptides, their complexation with alkali cations and the inhibition of the cation transport by 5,5-diphenylhydantoin(DPH) were studied theoretically using ECEPP/2 and MM2 force field. Several low energy conformations of uncomplexed cyclic hexapepides are obtained, and they adopt compact conformations in which most amide hydrogens form intramolecular hydrogen bond to amide carbonyl oxygens. The complexation energy of the peptide with $Na^+$ ion and DPH is -60 kcaal/mol and -18 kcal/mol, respectively. However, no suitable cavity to bind metal cation exists for the local minima of the peptide, and the internal energy of the uncomplexed hexapeptide having cavity is higher than that of the uncomplexed global minimum of this work by 10 kcal/mol. Also, one of the most important amino acid residue to bind DPH is Glycine, and this can explain experimental observation that the replacement of Gly by Sarcosine (N-methyl Glycine) reduce the inhibition ability of the cation transport.

• Journal of the Korean Wood Science and Technology
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• v.48 no.1
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• pp.32-40
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• 2020

While the utilization of wood as a raw material in related industries has been increasing with the population increasing, the availability of wood from natural forests has continued to decline. An alternative to this situation is the manufacture of particleboard from non-wood lignocellulose materials through the modification of sandwich particleboard (SPb) using bamboo strands as reinforcement. In this study, strandsof belangke bamboo (Gigantochloa pruriens W) and tali bamboo (Gigantochloa apus) were utilized. The non-wood particles included sugar palm fibers, cornstalk, and sugarcane bagasse. The board was made in a three-layer composition of the face, back, and core in a ratio of 1: 2: 1. The binder used was 8% isocyanate resin. The sheet was pressed at a temperature of 160℃ for 5 min under a pressure of 3.0 N/㎟. Testing included physical and mechanical properties based on the JIS A 5908 (2003) standard, while acoustic testing was based on ISO 11654 (1997) standards. The results showed that using bamboo strands as reinforcement has an effect on the mechanical and physical properties of SPb. Almost all the types of boards met the JIS A 5908 (2003) standards, with the exception of thickness swelling (TS) and internal bond (IB) parameters. Based on the thickness swelling parameter, the C-type board exhibited the best properties. Overall, the B-type board thatused a belangke bamboo strand for the surface and sugarcane bagasse as the core underwent the best treatment. Based on the acoustical parameter, boards using a tali bamboo strand for the surface and sugar palm fiber as the core (E-type board) exhibited good sound absorption properties.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2079-2094
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• 2018

Sunflower trypsin inhibitor (SFTI) is a 14-amino-acid bicyclic peptide that contains a single internal disulfide bond. We initially constructed chimeras of SFTI with N-terminal secretion signals from the Escherichia coli OmpA and Pseudomonas aeruginosa ToxA, but only detected small amounts of protease inhibition resulting from these constructs. A substantially higher degree of protease inhibition was detected from a C-terminal SFTI fusion with E. coli YebF, which radiated more than a centimeter from an individual colony of E. coli using a culture-based inhibitor assay. Inhibitory activity was further improved in YebF-SFTI fusions by the addition of a trypsin cleavage signal immediately upstream of SFTI, and resulted in production of a 14-amino-acid, disulfide-bonded SFTI free in the culture supernatant. To assess the potential of the secreted SFTI to protect the ability of a cytotoxic protein to kill tumor cells, we utilized a tumor-selective form of the Pseudomonas ToxA (OTG-PE38K) alone and expressed as a polycistronic construct with YebF-SFTI in the tumor-targeted Salmonella VNP20009. When we assessed the ability of toxin-containing culture supernatants to kill MDA-MB-468 breast cancer cells, the untreated OTG-PE38K was able to eliminate all detectable tumor cells, while pretreatment with trypsin resulted in the complete loss of anticancer cytotoxicity. However, when OTG-PE38K was co-expressed with YebF-SFTI, cytotoxicity was completely retained in the presence of trypsin. These data demonstrate SFTI chimeras are secreted in a functional form and that co-expression of protease inhibitors with therapeutic proteins by tumor-targeted bacteria has the potential to enhance the activity of therapeutic proteins by suppressing their degradation within a proteolytic environment.

• Journal of the Korean Wood Science and Technology
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• v.32 no.2
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• pp.65-72
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• 2004

A melamine-urea-formaldehyde (MUF) resin, based on 5 percent melamine addition of the resin solids weight, was synthesized in the laboratory for particleboard (PB) manufacture. Laboratory PBs were made with the MUF resin at three press times (3, 4, 5 min) and two resin application rates (6, 8 percent). Enclosed caul system was used for collecting the exhaust gases materials generated during the hot-pressing of PBs. Exhaust gases materials generated inside the enclosed caul during the hot-pressing of PBs were collected in a controlled air stream. Formaldehyde from the exhaust gases collected was determined per a chromotropic method of the National Institute of Occupational Safety and Health Method 3500. The measurement results showed that formaldehyde emissions during the hot-pressing of PB significantly increased with increasing press time, and MUF resin application rates. PB' performance test results showed that internal bond (IB) of PB made with 3-minute press time exceeded the minimum requirement for KS F 3104 PB type 8.0.

• Structural Engineering and Mechanics
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• v.85 no.3
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• pp.337-351
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• 2023

In this paper, the shear behavior of soft filling in rectangular-hollow concrete specimens was simulated using the 2D particle flow code (PFC2D). The laboratory-measured properties were used to calibrate some PFC2D micro-properties for modeling the behavior of geo-materials. The dimensions of prepared and modeled samples were 100 mm×100 mm. Some disc type narrow bands were removed from the central part of the model and different lengths of bridge areas (i.e., the distance between internal tips of two joints) with lengths of 30 mm, 50 mm, and 70 mm were produced. Then, the middle of the rectangular hollow was filled with cement material. Three filling sizes with dimensions of 5 mm×5 mm, 10 mm×5 mm, and 15 mm×5 mm were provided for different modeled samples. The parallel bond model was used to calibrate and re-produce these modeled specimens. Therefore, totally, 9 different types of samples were designed for the shear tests in PFC2D. The shear load was gradually applied to the model under a constant loading condition of 3 MPa (σc/3). The loading was continued till shear failure occur in the modeled concrete specimens. It has been shown that both tensile and shear cracks may occur in the fillings. The shear cracks mainly initiated from the crack (joint) tips and coalesced with another one. The shear displacements and shear strengths were both increased as the filling dimensions increased (for the case of a bridge area with a particular fixed length).

• Journal of the Korean Wood Science and Technology
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• v.32 no.1
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• pp.67-72
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• 2004

Wood/polyethylene panels were manufactured from Populus alba × glandulosa particles and low density polyethylene particles at three mixing rates, 50:50, 60:40, and 70:30. A total of 15 wood/polyethylene panels was made at 145℃ and 5 minutes hot-press time. Wood/polyethylene panels were tested for internal bond, bending, and dimensional stabilities such as thickness swell and water absorption. Panel performance data were analyzed using the SAS programing package. The test results of the wood/polyethylene panels showed that as the polyethylene mixing rates were increased, the panel property values increased. Based on panels' dimensional stabilities, the optimum wood/polyethylene mixing ratio appeared to be 60:40.