• Title/Summary/Keyword: interaction sites

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The Topic-Rank Technique for Enhancing the Performance of Blog Retrieval (블로그 검색 성능 향상을 위한 주제-랭크 기법)

  • Shin, Hyeon-Il;Yun, Un-Il;Ryu, Keun-Ho
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.1
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    • pp.19-29
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    • 2011
  • As people have heightened attention to blogs that are individual media, a variety rank algorithms was proposed for the blog search. These algorithms was modified for structural features of blogs that differ from typical web sites, and measured blogs' reputations or popularities based on the interaction results like links, comments or trackbacks and reflected in the search system. But actual blog search systems use not only blog-ranks but also search words, a time factor and so on. Nevertheless, those might not produce desirable results. In this paper, we suggest a topic-rank technique, which can find blogs that have significant degrees of association with topics. This technique is a method which ranks the relations between blogs and indexed words of blog posts as well as the topics representing blog posts. The blog rankings of correlations with search words are can be effectively computed in the blog retrieval by the proposed technique. After comparing precisions and coverage ratios of our blog retrieval system which applis our proposed topic-rank technique, we know that the performance of the blog retrieval system using topic-rank technique is more effective than others.

Targeting Analysis of Lumenal Proteins of Chloroplast of Wheat using Proteomic Techniques

  • Kamal, Abu Hena Mostafa;Kim, Da-Eun;Oh, Myoung-Won;Chung, Keun-Yook;Cho, Yong-Gu;Kim, Hong-Sig;Song, Beom-Heon;Lee, Chul-Won;Uozumi, Nobuyuki;Choi, Jong-Soon;Cho, Kun;Woo, Sun-Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2010.05a
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    • pp.14-14
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    • 2010
  • Plastid proteomics are essential organelles present in virtually all cells in plants and green algae. Plastids are responsible for the synthesis and storage of key molecules required for the basic architecture and functions of plant cells. The proteome of plastid, and in particular of chloroplast, have received significant amounts of attention in recent years. Various fractionation and mass spectrometry (MS) techniques have been applied to catalogue the chloroplast proteome and its sub-organelles compartments. To better understanding the function of the lumenal sub-organelles within the thylakoid network, we have carried out a systematical analysis and identification of the lumenal proteins in the thylakoid of wheat by using Tricine-SDS-PAGE, and LTQ-ESI-FTICR mass spectrometry followed by SWISS-PROT database searching. We isolation and fractionation these membrane from fully developed wheat leaves using a combination of differential and gradient centrifugation couple to high speed ultra-centrifuge. After collecting all proteins to eliminate possible same proteins, we estimated that there are 407 different proteins including chloroplast, chloroplast stroma, lumenal, and thylakoid membrane proteins excluding 20 proteins, which were identified in nucleus, cytoplasm and mitochondria. A combination of these three programs (PSORT, TargetP, TMHMM, and TOPPRED) was found to provide a useful tool for evaluating chloroplast localization, transit peptide, transmembranes, and also could reveal possible alternative processing sites and dual targeting. Finally, we report also sub-cellular location specific protein interaction network using Cytoscape software, which provides further insight into the biochemical pathways of photosynthesis. The present work helps understanding photosynthesis process in wheat at the molecular level and provides a new overview of the biochemical machinery of the thylakoid in wheat.

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Interaction of Antihistaminics with Muscarinic Receptor(II) -Action on the cerebral muscarinic $M_1$ Receptor- (항 Histamine제와 Muscarinic Receptor와의 상호작용(II) -대뇌 Muscarinic $M_1$ Receptor에 대한 작용-)

  • Lee, Shin-Woong;Park, Young-Joo;Park, In-Sook;Lee, Jeung-Soo
    • YAKHAK HOEJI
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    • v.34 no.4
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    • pp.224-237
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    • 1990
  • A single uniform population of specific, saturable, high affinity binding site of $[^3H]QNB$ guinuclidinyl benzilate(QNB) was identified in the rat cerebral microsomes. The Kd value(37.2 pM) for $[^3H]QNB$ calculated from the kinetically derived rate constants was in agreement with the Kd value(48.9 pM) determined by analysis of saturation isotherms at various receptor concentrations. Dimenhydrinate(DMH), histamine $H_1-blocker$, increased Kd value for $[^3H]QNB$ QNB without affecting the binding site concentrations and this effect resulted from the ability of DMH to slow $[^3H]QNB-receptor$ association. Pirenzepine inhibition curve of $[^3H]QNB$ binding was shallow(nH = 0.52) indicating the presence of two receptor subtypes with high ($M_1-site$) and low($M_2-site$) affinity for pirenzepine. Analysis of these inhibition curves yielded that 68% of the total receptor populations were of the $M_1-subtype$ and the remaining 32% of the $M_2-subtype$. Ki values for the $M_1-$ and $M_2-subtypes$ were 2.42 nM and 629.3 nM, respectively. Ki values for $H_1-blockers$ that inhibited $[^3H]QNB$ binding varied with a wide range ($0.02-2.5\;{\mu}M$). The Pseudo-Hill coefficients for inhibition of $[^3H]QNB$ binding by most of $H_1-blockers$ examined except for oxomemazine inhibition of $[^3H]QNB$ binding were close to one. The inhibition curve for oxomemazine in competition with $[^3H]QNB$ was shallow(nH = 0.74) indicating the presence of two receptor populations with different affinities for this drug. The proportion of high and low affinity was 33:67. The Ki values for oxomemazine were $0.045{\pm}0.016\;{\mu}M$ for high affinity and $1.145{\pm}0.232\;{\mu}M$ for low affinity sites. These data indicate that muscarinic receptor blocking potency of $H_1-blockers$ varies widely between different drugs and that most of $H_1-blockers$ examined are nonselective antagonist for the muscarinic receptor subtypes, whereas oxomemazine might be capable of distinguishing between subclasses of muscarinic receptor.

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A Comparative Study on the Perception of Actual Utilization of Smart Devices and Development of Culinary Education Application - Focused on 4-year University Students Located in the Daejeon.Chungnam Areas - (스마트 기기 활용 실태와 조리실습교육 애플리케이션 개발에 대한 인식 비교 연구 - 대전.충남지역 4년제 대학생을 중심으로 -)

  • Kang, Keoung-Shim
    • Culinary science and hospitality research
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    • v.19 no.2
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    • pp.176-189
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    • 2013
  • This study has been conducted on 213 students in 4-year universities located in the regions of Daejeon and Chungnam in order to investigate a method to develop a smart device based culinary education application and the results and development method were as follows. First, the most often used smart device was a smart phone, which is used for over 5 hours a day and mainly used for SNS. Second, they utilized a smart device for language and major study during their spare time, wanted educational contents most and thought them useful for learning. Third, most of the students were positively aware of the necessity and learning effects of culinary education applications, and the response rate to utilize the application once a week was highest. Also, they hoped various recipes and simple cuisine and craftsman cooking. Therefore, the functions of SNS mostly often used by students should be added to promote interaction between teachers and students. And more contents should be made for students to use easily in moving or in their spare time. Furthermore, various videos of teaching and theoretical information should be included. And the applications focused on recipes and simple and craftsman cooking should be developed and uploaded on a school homepage and on popular portal sites so that students can easily utilize them.

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Effect of Asphalt Pavement Conditions on Tensile Adhesive Strength of Waterproofing System on Concrete Bridge Deck (아스팔트 포장 조건이 교면방수 시스템의 인장접착강도에 미치는 영향)

  • Lee, Byung-Duck;Park, Sung-Ki;Kim, Kwang-Woo
    • International Journal of Highway Engineering
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    • v.5 no.2 s.16
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    • pp.15-24
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    • 2003
  • The performance of waterproofing system (WPS) is known to be a function of many complex interaction of material factors, design details, and the quality of construction, but it is mainly determined by the bond strength, which is measured by tensile adhesive strength (TAS) test. to the concrete bridge deck. In this research, eight waterproofing membranes were selected from commercial market and the tensile adhesive characteristics of the WPS on concrete bridge deck were investigated in view of various factor in asphalt pavement. The factors include type of asphalt mixture, pavement thickness, paving temperature and influence of wheel loading. TAS test of different asphalt pavement types showed that TAS of WPS under SMA (Stone Mastic Asphalt) pavement was greater than that under dense asphalt pavement. TAS of sheet membranes was improved as the compaction temperature of asphalt concrete increase, but TAS of liquid membranes were not. The influence of thickness of pavement val minimal with given laboratory test condition. TAS of sheet membranes after wheel tracking test were in the order of the sites under wheel path (UWP), before wheel tracking (BWT) and nearby wheel path (NWP). Since TAS of the same WPS of UWP was higher than TAS of BWT, wheel loading had function of pressing WPS resulting in higher adhesive strength. But liquid membranes were variable on types. The feature of detached interface after TAS test showed that sheet types were all detached in between deck concrete and WPS, and liquid types were detached in between asphalt pavement and WPS.

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Proton Conducting Composite Membranes Consisting of PVC-g-PSSA Graft Copolymer and Heteropolyacid (PVC-g-PSSA가지형 공중합체와 헤테로폴리산을 이용한 수소이온 전도성 복합 전해질막)

  • Kim, Jong-Hak;Koh, Jong-Kwan;Choi, Jin-Kyu;Yeon, Seung-Hyeon;Ahn, Ik-Sung;Park, Jin-Won
    • Membrane Journal
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    • v.19 no.2
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    • pp.96-103
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    • 2009
  • A series of organic-inorganic composite membranes from poly(vinyl chloride) (PVC) graft copolymer electrolyte and heteropolyacid (HPA) were prepared for proton conducting membranes. First, poly(vinyl chloride)-g-poly(styrene sulfonic acid) (PVC-g-PSSA) was synthesized by atom transfer radical polymerization (ATRP) using direct initiation of the secondary chlorines of PVC. HPA nanoparticles were then incorporated into the PVC-g-PSSA graft copolymer though the hydrogen bonding interactions, as confirmed by FT-IR spectroscopy. The proton conductivity of the composite membranes increased from 0.049 to 0.068 S/cm at room temperature with HPA contents up to 0.3 weight traction of HPA, presumably due to both the intrinsic conductivity of HPA particles and the enhanced acidity of the sulfonic acid of the graft copolymer. The water uptake decreased from 130 to 84% with the increase of HPA contents up to 0.45 of HPA weight traction, resulting from the decrease in number of water absorption sites due to hydrogen bonding interaction between the HPA particles and the polymer matrix. Thermal gravimetric analysis (TGA) demonstrated the enhancement of thermal stabilities of the composite membranes with increasing concentration of HPA.

Nickel Substitution Effects on Nano-sized Co, Mn and MnZn Ferrites Synthesized by Sol-gel Method

  • Choi, Won-Ok;Kwon, Woo Hyun;Chae, Kwang Pyo;Lee, Young Bae
    • Journal of Magnetics
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    • v.21 no.1
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    • pp.40-45
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    • 2016
  • Nickel substituted nano-sized ferrite powders, $Co_{1-x}Ni_xFe_2O_4$, $Mn_{1-x}Ni_xFe_2O_4$ and $Mn_{1-2x}Zn_xNi_xFe_2O_4$ ($0.0{\leq}x{\leq}0.2$), were fabricated using a sol-gel method, and their crystallographic and magnetic properties were subsequently compared. The lattice constants decreased as quantity of nickel substitution increased, while the particle size decreased in $Co_{1-x}Ni_xFe_2O_4$ ferrite but increased for the $Mn_{1-x}Ni_xFe_2O_4$ and $Mn_{1-2x}Zn_xNi_xFe_2O_4$ ferrites. For the $Co_{1-x}Ni_xFe_2O_4$ and $Mn_{1-x}Ni_xFe_2O_4$ ($0.0{\leq}x{\leq}0.2$) ferrite powders, the $M{\ddot{o}}ssbauer$ spectra could be fitted as the superposition of two Zeeman sextets due to the tetrahedral and octahedral sites of the $Fe^{3+}$ ions. However, the $M{\ddot{o}}ssbauer$ spectrum of $Mn_{0.8}Zn_{0.1}Ni_{0.1}Fe_2O_4$ consisted of two Zeeman sextets and one single quadrupole doublet due to the ferrimagnetic and paramagnetic behavior. The area ratio of the $M{\ddot{o}}ssbauer$ spectra could be used to determine the cation distribution equation, and we also explain the variation in the $M{\ddot{o}}ssbauer$ parameters by using this cation distribution equation, the superexchange interaction and the particle size. The saturation magnetization decreased in the $Co_{1-x}Ni_xFe_2O_4$ and $Mn_{1-2x}Zn_xNi_xFe_2O_4$ ferrites but increased in the $Mn_{1-x}Ni_xFe_2O_4$ ferrite with nickel substitution. The coercivity decreased in the $Co_{1-x}Ni_xFe_2O_4$ and $Mn_{1-2x}Zn_xNi_xFe_2O_4$ ferrites but increased in the $Mn_{1-x}Ni_xFe_2O_4$ ferrite with nickel substitution. These variations could thus be explained by using the site distribution equations, particle sizes and spin magnetic moments of the substituted ions.

Caspase-3 Specifically Cleaves $p21^{WAF1/CIP1}$ in the Earlier Stage of Apoptosis in SK-HEP-1 Human Hepatoma Cells

  • Park, Jeong-Ae;Kim, Kyu-Won;Kim, Shin-Il;Lee, Seung-Ki
    • Proceedings of the Ginseng society Conference
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    • 1998.06a
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    • pp.231-243
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    • 1998
  • In the present study, we provide evidence that ginsenoside $Rh_2$ (G-$Rh_2$) as well as staurosporine induces apoptosis of human hepatoma SK-HEP-1 cells by caspase 3-mediated processing of $p21^{WAFI/CIPI}$ in the early stage of apoptosls. Immunoblottings showed that G-$Rh_2$ as well as statrosporine induced the processing of caspase-3 to an active form, pl7. In stable Bcl-2 transfectants however, G-$Rh_2$ induced DNA fragmentation, while staurosporine did not. In the early stage of apoptosis, $p21^{WAFI/CIPI}$ was detected to undergo proteolytic processing specifically conducted by caspase-3. $p21^{WAFI/CIPI}$ translated in vitro was cleaved into a p14 fragment, when incubated with cell extracts obtained from either G-$Rh_2$- or staurosporine-treated cells. Cleavage was equally inhibited in both cases by adding Ac-DEVD-cho, a specific caspase-3 inhibitor, but not by Ac-YVkD-cho, a specific caspase-l inhibitor. Similarly, $p21^{WAFI/CIPI}$ was efficiently leaved by recombinant caspase-3 overexpressed in E. coli. Moreover, the endogenous $p21^{WAFI/CIPI}$ of untreated-cell extracts was also cleaved by recombinant caspase-3. Mutation analysis allowed identification of two caspase-3 cleavage sites, $DHVD^{112}$/L and $SMTD^{149}$/F, which are located within, or near the interaction domains for cyclins, Cdks, and PCNA. Taken together, these results show that G-$Rh_2$ as well as staurosporine increases caspase-3 activity, which in turn directly cleaves $p21^{WAFI/CIPI}$ resulting in elevation of Cdk kinase activity in the early stages of apoptosis. We propose that proteolytic cleavage of $p21^{WAFI/CIPI}$ is a functionally relevant event that allows unleashing the cyclin/Cdk activity from the inhibitor seen in the earlier stage of apoptosis, the event of which may be associated with the triggering mechanism for the execution of apoptosis.

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Analysis of Site Amplification of Seismic Stations using Odesan Earthquake (오대산지진 자료를 이용한 국내 지진관측소 부지의 지반증폭특성 연구)

  • Kim, Jun-Kyoung
    • Journal of the Earthquake Engineering Society of Korea
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    • v.13 no.1
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    • pp.27-34
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    • 2009
  • Site amplification should be considered in order to estimate Soil-Structure Interaction (SSI), seismic source and attenuation parameters with a greater degree of reliability. The horizontal to vertical (H/V) ratio technique, originally proposed by Nakamura (1989), has been applied to analyze the surface waves in microtremor records. Recently, its application has been extended to the shear wave energy of strong motion in order to study the site transfer function. The purpose of this paper is to estimate the H/V spectral ratio using the observed data from 9 seismic stations distributed within the Southern Korean Peninsula, from the Odesan earthquake (2007/01/20). The results show that most of the stations have more stable amplification characteristics in a low frequency band than in a high frequency band. However, each seismic station showed its own characteristic resonant frequency and low and high frequency. The resonant frequency at each station should be estimated carefully, because the quality of seismic data is dependent on the resonant frequency. It can be obtained more reliable results of seismic source and attenuation parameters, if seismic ground motions which deconvolved from site transfer function is used. The site amplification data from this study can be used to generally classify the sites within the Southern Korean Peninsula.

Interactions between Pine Needle Gall Midge, Thecodiplosis japonensis (Diptera: Cecidomyiidae), and its Parasitoids in Newly Invaded Areas (솔잎혹파리 확산지역에서 솔잎혹파리와 기생봉의 상호작용)

  • 박영석;정여진;전태수;이범영;이준호
    • Korean journal of applied entomology
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    • v.40 no.4
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    • pp.301-307
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    • 2001
  • The pine needle gall midge (PNGM), Thecodiplosis japonensis Uchida et Inouye, is a serious pest in pine forests in Korea. Since the first report of PNGM infestation in Korea in 1929, the infestation area has been expanded gradually. In 1975 the distribution of PNGM and its parasitoids were surveyed throughout several infested areas in Korea. Annual survey has been made for the rates of gall formation by PNGM and parasitism by its parasitoids at the monitoring sites including newly infested area in 1975 since 1980. These data were used to examine the relationship between PNGM and its parasitoids for newly invaded areas. The gall forming rate of PNGM and the parasitism by the parasitoid were 34.8 and 1.9% in 1975, respectively, while the gall forming rate and the parasitism were 20.7 and 8.9% in 1985, respectively. The relationships of densities between PNGM and its parsitoid were weak in the early stage of dispersion, but the density of parasitoid was with an asymptotic increase along with PNGMs density increase during the observation period for 10 years.

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