• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1323-1329
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• 2005

A mathematical model was developed for predicting the growth kinetics of Listeria monocytogenes in tryptic soy broth (TSB) as a function of combined effects of temperature, pH, and NaCl. The TSB containing four different concentrations of NaCl (2, 4, 5, and $10\%$) was initially adjusted to six different pH levels (pH 5, 6, 7, 8, 9, and 10) and incubated at 4, 10, 25, or 37$^{circ}C$. In all experimental variables, the primary growth curves were well fitted ($r^{2}$=0.982 to 0.998) to a Gompertz equation to obtain the lag time (LT) and specific growth rate (SGR). Surface response models were identified as appropriate secondary models for LT and SGR on the basis of coefficient determination ($r^{2}$=0.907 for LT, 0.964 for SGR), mean square error (MSE=3.389 for LT, 0.018 for SGR), bias factor ($B_{1}$B,=0.706 for LT, 0.836 for SGR), and accuracy factor ($A_{f}$=1.567 for LT, 1.213 for SGR). Therefore, the developed secondary model proved reliable predictions of the combined effect of temperature, NaCl, and pH on both LT and SGR for L. monocytogenes in TSB.

• Journal of Korean Ophthalmic Optics Society
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• v.15 no.3
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• pp.293-297
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• 2010

Purpose: The aim of the current study was to compare body mass index (BMI) with waist circumference (WC) as a predictor of elevated intraocular pressure (IOP). Methods: The subjects were consisted of 458 adults, aged 20 year or above, of one community in Kyunggi-do. Mean IOPs were stratified jointly for BMI and WC tertiles. Multi-variate linear regression analysis was also used to compare between BMI and WC. Results: Although any BMI tertiles were not associated with IOP within each tertile of WC, WC tertiles was significantly related to elevation of IOP within the third BMI tertile (${\geq}24.9kg/m^2$). After adjusting for age and sex, only WC showed significant association with IOP. In additional adjustment for lifestyle variables, both BMI and WC were significantly associated with elevation of IOP. However, the results showed the stronger association of IOP with WC than BMI, whether they were adjusted by age and sex or additionally lifestyle variables. Conclusions: These data showed that BMI and WC were positively associated with IOP. However, WC appeared to be a better indicator for higher IOP than BMI.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.185-205
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• 2017

The purpose of this research is to examine the relationship between the adult attachment of married employees, their job motivation and marital relationship. For this purpose, a questionnaire survey was carried out with 370 married employees in the Chungnam area as the target from Sep. 10 to Oct. 14, 2016. The collected data was analyzed using SPSS 23.0 using descriptive statistical analysis, the independent sample T-test, ANOVA analysis, Pearson correlation, and structural equation analysis. First, significant differences were revealed in the level of attachment avoidance, satisfaction with the marriage relationship, and job motivation according to the age group, in the level of attachment avoidance and satisfaction with the marriage relationship according to the education level, in the level of attachment avoidance and satisfaction with the marriage relationship according to the marriage period, in job motivation according to the position, in satisfaction with the marriage relationship according to the average household income, and in satisfaction with the marriage relationship according to the work shift pattern. Second, although a significant negative correlation appeared between attachment anxiety and attachment avoidance, a significant positive correlation was found between attachment avoidance and job motivation. Also, although a significant negative correlation appeared between attachment avoidance and marital relationship, a significant positive correlation was found between job motivation and marital relationship. Third, to examine the mediating effect of satisfaction with the marital relationship on the relationship between adult attachment and job motivation, a structural equation analysis was carried out. As a result, the indirect effect of attachment anxiety on job motivation turned out to be significant and its complete mediating effect was confirmed. This suggests that when developing programs to improve job motivation within the workplace or consultative interventions for the enhancement of marital relationships, the above characteristics of individuals should be taken into consideration.

• Women's Health Nursing
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• v.5 no.3
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• pp.313-326
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• 1999

This study sought to find out the level of perception of postpartal care and the meaning and opinion of traditional postpartal care (Sanhujori) from health professional. The subjects were 188 health professionals who work at University hospital, clinics, Oriented clinics, and midwifery clinics in Seoul and Chung-Buk, Korea. Data were collected from 8th April, 1999 to 6th May, 1999. The data were analyzed through the SPSS program by use of frequency, percentage, t-test, ANOVA and Scheffe test as a post hoc contrast, $x^2$-test, and stepwise multiple regression. The results of the study were as follows: mean age of the subject was 33.0 years and female was 58.0%. Occupation of the subject was nurse 28.7%, doctor 22.3%, midwife 21.3 Korean Oriental doctor 27.0%. Mean period of career was 7.6 years and the subject who experienced Sanhujori was 54.3%. The subject who did not know 6 principles of Sanhujori was 73.9%. In the opinion on traditional Sanhujori method 68.1% of respondents expressed that Sanhujori is scientific postpartal care which fits Korean people's physical constitution and culture. On the opinion of effective postpartal care in 97.9% of respondents expressed that it is to in hospital postpartal care and traditional Sanhujori perform it according to Korean culture constitution. On the opinion of Sanhujori, 96.6% of respondents expressed that it is necessary to understand Sanhujori with consideration which was adapted to Oriental culture and to verify it through continual study. On the opinion of the effect of the level of performance of Sanhujori upon women's health life, 43.2% of respondents expressed that the level of performance of Sanhujori has great effect on women's general health, including postpartal recovery, Sanhujori prevention, and so on. On the opinion of Sanhujori of women who undergo Caesarean operation, 57.7% of respondents expressed that women who undergo Caesarean operation perform Sanhujori with more attention. On the opinion of Sanhujori center (sanhujorwon), 56.3% of respondents expressed that the center is necessary for women's health and health professional are required to manage the center scientifically. On considering the level of importance of post care according to occupation, midwife showed highest level of importance of traditional Sanhujori doctor the lowest level. Midwife showed the hi level of importance of postpartal care and K Oriental doctor showed the lowest level of importance of women's postpartal care. On considering the relationship between the level of importance of postpartal care and general characteristics, sex, career, clinical department and whether they know 6 principles of Sanhujori or not were statistically significant at the level of 5%. In the stepwise multiple regression analysis, the main influencing variables on the level of importance of postpartal care were occupation, sex, and clinical department. In conclusion, this finding confirmed that professionals were considering the traditional Sa importantly for women's health. Above all things necessary for health professional to integrate concept of traditional Sanhujori into practical nu intervention program, to apply it to profes practices in order to reestablish effective integrative postpartal caring system, and to Sanhujori scientific through performing continual research.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.27 no.4
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• pp.196-205
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• 2017

In recent years, the service design in the medical sector evolves through practical service research and development that can visualize both intangible and intangible service elements in an integrative way and derive innovative solutions to help customers feel the service more important value. With the improvement of personal income, interest in medical welfare and well-being is increasing day by day, and the focus of the medical sector shifts from the concept of treatment of diseases and illness to preventive medicine. In response to this trend, research and development of home health care system, which greatly reduces the time and space constraint of health checkup and health care by combining ubiquitous concept with medical welfare, are being actively conducted, and the needs for improving products and medical environment based on user-centered medical service and user needs in accordance with the Health Care 3.0 Era, it becomes necessary to develop on-site medical diagnostic products that reflect user-centered needs and needs. This study is intended to research and develop a product that sufficiently reflects the needs of users by applying suitable materials and shape for on-site diagnostic product in researching and developing Wireless X-ray Detector.

• Journal of Korean Neurosurgical Society
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• v.48 no.5
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• pp.391-398
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• 2010

Objective : This study was designed to validate the cell trafficking efficiency of the in vivo bioluminescence image (BLI) study in the setting of transplantation of the luciferase expressing bone marrow-derived mesenchymal stem cells (BMSC), which were delivered at each different time after transient middle cerebral artery occlusion (MCAO) in a mouse model. Methods : Transplanting donor BMSC were prepared by primary cell culture from transgenic mouse expressing luciferase (LUC). Transient focal infarcts were induced in 4-6-week-old male nude mice. The experiment mice were divided into five groups by the time of MSC transplantation : 1) sham-operation group, 2) 2-h group, 3) 1-day group, 4) 3-day group, and 5) 1-week group. BLI for detection of spatial distribution of transplanted MSC was performed by detecting emitted photons. Migration of the transplanted cells to the infarcted area was confirmed by histological examinations. Differences between groups were evaluated by paired t-test. Results : A focal spot of bioluminescence was observed at the injection site on the next day after transplantation by Signal intensity of bioluminescence. After 4 weeks, the mean signal intensities of 2-h, 1-day, 3-day, and 1-week group were $2.6{\times}10^7{\pm}7.4{\times}10^6$. $6.1{\times}10^6{\pm}1.2{\times}10^6$, $1.7{\times}10^6{\pm}4.4{\times}10^5$, and $8.9{\times}10^6{\pm}9.5{\times}10^5$, respectively. The 2-h group showed significantly higher signal intensity (p<0.01). The engrafted BMSC showed around the infarct border zones on immunohistochemical examination. The counts of LUC-positive cells revealed the highest number in the 2-h group, in agreement with the results of BLI experiments (p<0.01). Conclusion : In this study, the results suggested that the transplanted BMSC migrated to the infarct border zone in BLI study and the higher signal intensity of LUC-positive cells seen in 2 hrs after MSC transplantation in MCAO mouse model. In addition, noninvasive imaging in real time is an ideal method for tracking stem cell transplantation. This method can be widely applied to various research fields of cell transplantation therapy.

• Journal of Food Hygiene and Safety
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• v.22 no.1
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• pp.1-14
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• 2007

The term, "food safety", has traditionally been viewed as a practical science aimed at assuring the prevention acute illnesses caused by biological microorganisms, and only to a minor extent, chronic diseases cause by chronic low level exposures to natural and synthetic chemicals or pollutants. "food safety" meant to prevent microbiological agents/toxins in/on foods, due to contamination any where from "farm to Fork", from causing acute health effects, especially to the young, immune-compromised, genetically-predisposed and elderly. However, today a broader view must also include the fact that diet, perse (nutrients, vitamins/minerals, calories), as well as low level toxins and pollutant or supplemented synthetic chemicals, can alter gene expressions of stem/progenitor/terminally-differentiated cells, leading to chronic inflammation and other mal-functions that could lead to diseases such as cancer, diabetes, atherogenesis and possibly reproductive and neurological disorders. Understanding of the mechanisms by which natural or synthetic chemical toxins/toxicants, in/on food, interact with the pathogenesis of acute and chronic diseases, should lead to a "systems" approach to "food safety". Clearly, the interactions of diet/food with the genetic background, gender, and developmental state of the individual, together with (a) interactions of other endogenous/exogenous chemicals/drugs; (b) the specific biology of the cells being affected; (c) the mechanisms by which the presence or absence of toxins/toxicants and nutrients work to cause toxicities; and (d) how those mechanisms affect the pathogenesis of acute and/or chronic diseases, must be integrated into a "system" approach. Mechanisms of how toxins/toxicants cause cellular toxicities, such as mutagenesis; cytotoxicity and altered gene expression, must take into account (a) irreversible or reversal changes caused by these toxins or toxicants; (b)concepts of thresholds or no-thresholds of action; and (c) concepts of differential effects on stem cells, progenitor cells and terminally differentiated cells in different organs. This brief Commentary tries to illustrate this complex interaction between what is on/in foods with one disease, namely cancer. Since the understanding of cancer, while still incomplete, can shed light on the multiple ways that toxins/toxicants, as well as dietary modulation of nutrients/vitamins/metals/ calories, can either enhance or reduce the risk to cancer. In particular, diets that alter the embryo-fetal micro-environment might dramatically alter disease formation later in life. In effect "food safety" can not be assessed without understanding how food could be 'toxic', or how that mechanism of toxicity interacts with the pathogenesis of any disease.

• Journal of Life Science
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• v.26 no.1
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• pp.123-128
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• 2016

The object of this study was to obtain accurate information about the co-administration effects of cardiotonic pills on the pharmacokinetics of cilostazol were observed as a process of the comprehensive and integrative medicine. Cilostazol is a synthetic anti-platelet and vasodilator agent developed for the treatment of intermittent claudication resulting from peripheral arterial disease. By increasing intracellular cyclic adenosine monophosphate (cAMP), cilostazol induces the activation of protein kinase A, which activates endothelial nitric oxide synthase. In order to evaluate the effect of a single or repeated cardiotonic pill dose on the pharmacokinetics of cilostazol, a single dose of pure_distilled water or a colloidal suspension of distilled water and cardiotonic pills were administered to the control and test groups, respectively. After 30 min, both groups were administered cilostazol. Plasma was collected 30min before administration, and 0.25, 0.5, 0.45, 1, 2, 4, 6, 8, and 24h after the end of cilostazol treatment. We then evaluated the pharmacokinetic changes observed with cilostazol between the control and test groups. No statistically significant differences were observed. These findings demonstrated that a single dose of cardiotonic pills did not affect the pharmacokinetics of cilostazol. The results obtained in this study suggest that co-administration of cardiotonic pills and cilostazol may not affect the bioavailability of cilostazol as a potential drug interaction.

• The Korean Journal of Zoology
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• v.23 no.2
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• pp.61-68
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• 1980

Quantitative analysis of the activities of transport ATPases as well as alkaline phosphatase of the mouse uterus was carried out during the estrous cycle. Even though the proportional patterns of the enzyme activities were similar each another between the stages of estrous cycle, the absolute activities of the enzymes except $K^+$-dependent and $Na^+$, $K^+$-activated ATPases at the time of estrus were significantly (p<0.025) higher than that at any other time of the estrous cycle. That is, the activities of $K^+$-dependent and $Na^+$, $K^+$-activated ATPases were negligible during the period of time from diestrus to estrus while the little activities (0.04 $\\sim$ 0.05$\\mu$M/mg protein/hr in average, $6\\sim7$% of the total enzyme activity) of these enzymes appeared at the time of metaestrus. On the other hand, at the time of estrus, the activities of $Mg^++$-dependent phosphatase, transport ATPase and alkaline phosphatase were rapidly and tremendously increased to be 0.69 (35%), 0.42 (21%) and 1.58 (79%), respectively. The activity of alkaline phosphatase was in the range of 0.60 $\\sim$ 1.58 (79 $\\sim$ 90%) and predominant throughout the estrous cycle. The activity of $Mg^++$-dependent alkaline phosphatase was estimated as 12 $\\sim$ 16% of the total enzyme activity. Therefore, it is assumed likely that $K^+$-dependent and $Na^+$, $K^+$-activated ATPases are not the main factors to control the fluid accumulation at the time of estrus, but may be the factors to reabsorb the luminal fluid into the uterine epithelium at the time of metaestrus, and that $Mg^++$-dependent phosphatase, transport ATPase and alkaline phosphatase must be closely involved in the secretion of luminal fluid from the epithelial cells of the mouse uterus.

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.295-303
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• 2006

To provide microbial information for the safety of agricultural production, the presence of enterotoxin genes and antibiotic susceptibility of 14 isolated Staphylococcus aureus (11.7%) strains were investigated using PCR-based methods and disk diffusion method, respectively. Among enterotoxin-encoding genes, sea was detected from two isolates (14.3%), sea and sed genes were co-detected from three isolates (21.4%), and sea, sed, and see genes in seven isolates (50.0%), whereas seb, sec, and tsst were not detected in any isolate. Nine (64.3%), eight (57.1%), six (42.9%), two (14.3%), and one (7.2%) isolates were resistant to penicillin, novobiocin, amphicillin, erythromycin and oxacillin, and doxycycline and kanamycin, respectively. Methicilline-resistant S. aureus was found in roller of B farm and in hydroponic solution of D farm.