Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Characteristics of Enterotoxigenic genes and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Tomato Farms in Western Gyeongnam

서부 경남지역 토마토 농장에서 분리된 Staphylococcus aureus의 내독소 유전자의 특성과 항생제 감수성

  • Kim, Jin-Soo (Busan Regional Food & Drug Administration) ;
  • Lee, Jin-Ha (Division of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Ji-Hun (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, Ju-Mi (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Se-Ri (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee, Kyu-Ho (Department of Environmental Engineering and Biotechnology. Hankuk University of Foreign Studies) ;
  • Kim, Min-Gon (Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Cheol-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
  • 김진수 (부산식품의약품안전청) ;
  • 이진하 (강원대학교 바이오산업공학부) ;
  • 김지훈 (경상대학교 응용생명과학부) ;
  • 최주미 (경상대학교 응용생명과학부) ;
  • 김세리 (농촌진흥청 작물과학원 영남농업연구소) ;
  • 하상도 (중앙대학교 식품공학과) ;
  • 김근성 (중앙대학교 식품공학과) ;
  • 이규호 (한국외국어대학교 환경학과) ;
  • 김민곤 (한국생명공학연구원) ;
  • 김광엽 (충북대학교 식품공학과) ;
  • 김철호 (동국대학교 한의과대학) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Published : 2006.04.01
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Abstract

To provide microbial information for the safety of agricultural production, the presence of enterotoxin genes and antibiotic susceptibility of 14 isolated Staphylococcus aureus (11.7%) strains were investigated using PCR-based methods and disk diffusion method, respectively. Among enterotoxin-encoding genes, sea was detected from two isolates (14.3%), sea and sed genes were co-detected from three isolates (21.4%), and sea, sed, and see genes in seven isolates (50.0%), whereas seb, sec, and tsst were not detected in any isolate. Nine (64.3%), eight (57.1%), six (42.9%), two (14.3%), and one (7.2%) isolates were resistant to penicillin, novobiocin, amphicillin, erythromycin and oxacillin, and doxycycline and kanamycin, respectively. Methicilline-resistant S. aureus was found in roller of B farm and in hydroponic solution of D farm.

본 연구는 서부 경남지역 토마토 농장 5 곳에서 총 120점의 시료를 수집하여 S. aureus를 분리하였으며, 분리된 S. aureus 에대하여 PCR에 방법을 이용, 내독소 유전자(enterotoxin genes)와디스크 확산방법(disk diffusion method)을 이용하여 22가지 항생제에 대하여 항생제 감수성 실험을 수행하였다. 본 연구의 결과 14점의 시료(11.7%)가 S. aureus에 오염되어 있었다. 가장 빈번하게 검출된 시료로는 관개용수, 양액, 작업자의 손 그리고 토마토였다. 또한 14개의 시료에서 enterotoxin 유전자가 검출되었고, 이들 중 sea 유전자를 갖는 균주는 2주(14.3%), sea와 sed 유전자를 동시에 갖는 균주가 3주(21.4%), sea, sed 및 see 유전자가 모두 검출된 균주는 7주(50.0%)였다. 그러나 sec, sed와 tsst유전자를 갖는 균주는 검출되지 않았다. 분리된 균주를 대상으로 항생제 내성실험결과 penicillin에 대한 항생제 내성이 64.3%로 뚜렷하게 높은 것으로 나타났다. 그 외 novobiocin(57.1%)에 대하여 8 균주, amphicillin(42.9%)에 대하여 6균주, erythromycin(13.4%), oxacillin (14.2%)에 대하여 2 균주, kanamycin(7.2%), doxycycline(7.2%)에대하여 1 균주가 내성을 나타내었다. 특히 B 농장의 롤러와 D 농장의 양액에서 분리된 균주는 oxacillin에 내성을 갖는 MRSA(Methicillin Resistant S. aureur)균주였다. 따라서 본 연구결과는 안전 농산물 생산을 위한 미생물학적 정보를 제공할 수 있을 것으로 사료되는 바이다.

Keywords

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