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Characteristics of Enterotoxigenic genes and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Tomato Farms in Western Gyeongnam  

Kim, Jin-Soo (Busan Regional Food & Drug Administration)
Lee, Jin-Ha (Division of Biotechnology and Bioengineering, Kangwon National University)
Kim, Ji-Hun (Division of Applied Life Science, Gyeongsang National University)
Choi, Ju-Mi (Division of Applied Life Science, Gyeongsang National University)
Kim, Se-Ri (Yeongnam Agricultural Research Institute, NICS, RDA)
Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
Lee, Kyu-Ho (Department of Environmental Engineering and Biotechnology. Hankuk University of Foreign Studies)
Kim, Min-Gon (Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology)
Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University)
Kim, Cheol-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.2, 2006 , pp. 295-303 More about this Journal
Abstract
To provide microbial information for the safety of agricultural production, the presence of enterotoxin genes and antibiotic susceptibility of 14 isolated Staphylococcus aureus (11.7%) strains were investigated using PCR-based methods and disk diffusion method, respectively. Among enterotoxin-encoding genes, sea was detected from two isolates (14.3%), sea and sed genes were co-detected from three isolates (21.4%), and sea, sed, and see genes in seven isolates (50.0%), whereas seb, sec, and tsst were not detected in any isolate. Nine (64.3%), eight (57.1%), six (42.9%), two (14.3%), and one (7.2%) isolates were resistant to penicillin, novobiocin, amphicillin, erythromycin and oxacillin, and doxycycline and kanamycin, respectively. Methicilline-resistant S. aureus was found in roller of B farm and in hydroponic solution of D farm.
Keywords
Staphylococcus aureus; tomato farms; enterotoxin gene; antibiotic susceptibility;
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1 Speller DC, Johnson AP, James D, Marpes RR, Chalett A, Gorge RC. Resistance to methicillin and other antibiotics in isolated of Staphvlococcus aureus from blood and cerebrospinal fluid, England and Wales, 1989-95. Lancet 359: 323-325 (1997)
2 Park HO, Kim CM, Woo GJ, Park SH, Lee DH, Chang EJ, Park KH. Monitoring and trends analysis of food poisoning outbreaks occurred in recent years in Korea. J. Food. Hyg. Safety 16: 280-294 (2001)   과학기술학회마을
3 Harris LJ, Beuchat LR, Kajs TM, Ward TE, Taylor CH. Efficacy and reproducibility of a produce wash in killing Salmonellr: on the surface of tomatoes assessed with a proposed standard method for produces sanitizer. J. Food Prot. 64: 1447-1485 (2001)
4 Matsunaga T, Kamata S, Kakiichi N, Uchida K. Characteristics of Staphvlococcus aureus isolated from peracute, acute and chronic bovine mastitis. J. Vet. Med. Sci. 55: 297-300 (1993)   DOI   ScienceOn
5 Jeong HB. Cho MA, No JR, Kang NJ, Jeong HJ. Kim HT, Kang KY. 'Tamnara', a new full ripe tomato for protected cultivation. Korean J. Hort. Sci. Technol. 22: 25-28 (2004)
6 Anonymous. Guidelines for effectiveness testing of surgical hand scrub (glove juice test). Fed. Regist. 43: 1242-1243 (1978)
7 NCCLS. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 6th ed. M7-A6. NCCLS, Wayne, PA. USA (2003)
8 Kim SR, Shim WB, Kim JH, Hwang SJ, Park SJ, Ha SD, Kim KS, Lee KH, Kim MG Kim KY, Kim CH, Chung DH. Screeing of Staphvlococcus aureus and Staphylococcal enterotoxin a, b, c gene in strain isolated from strawberry farms in Western Gyeongnam. Korean J. Food Sci. Technol. 37: 321-327 (2005)   과학기술학회마을
9 Norman NN. Wang LL. Studies on the use of sewage effluent for irrigation of truck crops. J. Milk Food Technol. 24: 44-47 (1961)
10 Gambrill MP, Mara DD, Silva SA. Physiochemical treatment of' tropical wastewater: production of microbiologically safe effluents for unrestricted crop irrigation. Water Sci. Technol. 26: 1449-1458 (1992)
11 Martin MC, Fueyo JM, Gonzalez-Hevia MA. Mendoza MC. Genetic procedures for identification of enterotoxigenic stains of Staphvlococcus aureus from three food poisoning outbreaks. Int. J. Food Microbiol. 94: 279-286 (2004)   DOI   ScienceOn
12 Cho SJ, Park CJ, Oem DI. Soil science. Hyangmunsa Co., Seoul. Korea. pp. 109-128 (1996)
13 Jung HJ, Cho JI, Park SH, Ha SD, Lee KH, Kim CH, Song FS, Chung DH. Kim MG, Kim KY, Kim KS, Genotypic and phenotypic characteristics of Staphylococcus aureus isolates from lettuces and raw milk. Korean J. Food Sci. technol. 37: 134-141 (2005)   과학기술학회마을
14 Ha DJ, Kim YC, Kim YJ. Management or infection for methicillin resistant Staphylococcus aureus at an orthopaedic surgery department. J. Korean Orthop. Assoc. 38: 34-38 (2003)   DOI
15 Chen TR, Hsiao MH, Chiou CS, Tsen HY. Development and use of PC R primers for the investigation of $C_1$, $C_2$ and $C_3$, enterotoxin types of Staphylococcus aureus strains isolated from food-borne outbreaks. Int. J. Food Microbiol. 71: 63-70 (2001)   DOI   ScienceOn
16 Bisbini P, Leoni E. Nanetti A. An outbreak of Salmollella harbor associated with roast rabbit in a restaurant. Eur. J. Epidemiol. 16: 613-618 (2000)   DOI   ScienceOn
17 Centers for disease control and prevention. Outbreaks of Salmonella infections associated with eating Roma tomatoes-United states and Canada, 2004. Available from: http://www.ede.gov. Accessed April 8, 2005
18 Tsen HY, Yu Gk, Wang KC, Wang SJ, Chang MY, Lin LY. Comparison of the enterotoxigenic types, toxic shock syndrome toxin 1 (TSST-1) strains and antibiotic susceptibilities for enterotoxigenic Staphylococcus aureus strains isolated from food and clinical samples. Food Microbiol. 15: 33-41 (1998)   DOI   ScienceOn
19 Kim HJ, Park JK, Lee DS, Paik HD. Changes of indicator microorganisms and pathogenic bacteria in spinach during cook-chill process. Korean J. Food Sci. Technol. 34: 927-930 (2002)   과학기술학회마을
20 Park SG, Hwang YO, Jung JH, Lee KM. Biological characteristics of Staphylococcus aureus isolated from food-borne patients in Seoul. J. Food Hyg. Safety 16: 159-167 (2001)   과학기술학회마을
21 Lee YW, Park SG. Distribution of indicator organisms and influence of storage temperature and period in commercial plant food. J. Food Hyg. Safety 14: 1-8 (1999)
22 Zshock M, Botzler D, Blocher S, Sommerhauser J, Hamann HP. Detection of genes for enterotoxins (ent) and toxic shock sysdrome toxin-1 (tsst) in mammary isolates of Staphylococcus aureus by polymerase-chain-reacton. Int. Dairy J. 10: 569-574 (2000)   DOI   ScienceOn
23 Hazariwala A, Sanders Q, Hudson CR, Hofacre C, Thayer SG, Distribution of staphylococcal enterotoxin genes among Staphylococcus aureus isolates from poultry and human invasive staphylococcal disease. Avian Dis. 46: 132-136   DOI
24 Baiocchi P, Galie M, Santini C, Czarfagna P, Cassone M, Tarasi D, Venditti M. In vitro susceptibility of Staphylococcus aurcus isolated from blood to currently used anti staphylococcal drug. J. Chemother. 10: 25-28 (1998)   DOI
25 Kim SR, Park SJ, Shim WB, Kim HK, Chung DH. Detection of Staphylococcus aureus and screening Staphylococcal enterotoxin a, b, c genes in strains isolated from strawberry juice shops in Jin-ju. Korean J. Env. Hlth. 31: 23-30 (2005)   과학기술학회마을
26 FDA. Center for Food Safety and Appied Nutrition. FDA survey of imported fresh interim results. Avaiable from http://www.cfsan.Fda.gov./~dms/prodsur9.html. Accessed July 31, 2001
27 Brown MH, Meat microbiology. Applied Science Publishers. London, England. pp. 269-486 (1982)
28 Klotz M, Opper S, Heeg K, Zimmermann S. Detection of Staphylococcus aureus enterotoxins A to D by real-time fluorescence PCR assay. J. Clin. Microbiol. 41: 4683-4687 (2003)   DOI
29 Sveum WH, Moberg LJ, Rude RA, Frank JF. Microbiological monitoring of the food processing environment. 3rd ed. American Public Health Association, Washington D.C., USA. pp. 51-74 (1992)
30 Jang DS, Sin DH, Chung DH, Kim CM, Lee IS. Food hygienic. Jeongmungak Co., Seoul, Korea, pp. 71-111 (2002)
31 Kang SM, Crops Physiology, Hyangmunsa Co., Seoul, Korea. pp. 77-127 (1996)
32 Choi JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, Kim MG, Lee DH, Kim KS, Ha SD. Microbial contamination levels of fresh vegetables distrihuted in markets. J. Food. Hyg. Safety 20: 43-47 (2005)   과학기술학회마을
33 Kim SY. Indication system for the verification in agricultural food safety. pp. 23-42. In: Symposium on GAP application strategy for the safety agricultural production. July 29, Gyeongsang National University, Jinju, Korea. The Center of Agri-food Safety. Jinju, Korea (2004)
34 Monday SR, Bohach GA. Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in Staphylococcal isolates. J. Clin. Micobiol. 44: 3411-3414 (1999)
35 Johnson WM, Tyler SD, Ewan EP, Ashton FE, Pollard DR, Rozee KR. Detection of genes for enterotoxins, exfoliative toxins and toxic shock syndrome toxin-1 in S. aureus by the polymerase chain reaction. J. Clin. Microbiol. 29:426-430 (1991)
36 Kaneko KI, Hideki H, Yoshimitsu O, Junko K, Masahiko K, Koki T, Yasuo S, Masua O. Bacterial contamination of ready-to-cat foods and fresh products in retail shops and food factories. J. Food Prot. 62: 644-649 (1999)   DOI
37 Ha KS, Park SJ, Shim WB, Chung DH. Screening of MRSA (Methicilline Resistant Staphylococcus Aureus) and seb gene in producing strains isolated from food service environment of elementary schools. J. Food. Hyg. Safety 18: 79-86 (2003)
38 Tsen HY, Chen TR. Use of the polymerase chain reaction for specific detection of type A, D, and E enterotoxigenic Staphvlococcus aureus in foods, Appl. Microbiol. Biotcchnol. 37: 685-690 (1992)
39 Suk SU, Park SC. Staphylococcal infections. J. Infection 17: 115-122 (1985)
40 Bell WA, Marpuis RE. Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Appl. Environ. Microbiol. 57: 1134-1138 (1991)
41 Boehme S, Werner G, Klare I, Reissbrodt R, Witte W. Occurrence of antibiotic-resistant enterobacteria in agricultural foodstuffs. Mol. Nutr. Food Res. 48: 522-531 (2004)   DOI   ScienceOn
42 AOAC Official Method of Analysis of AOAC Intl. 17th ed. Method 987.09. Association of Official Analytical Chemists, Arlington. VA, USA (2000)
43 Becker K, Roth R, Peters G, Rapid and specific detection of toxic Staphylococcus aureus: Use of two multiplex PCR enzyme immunoassays for amplification and hybridization of Staphylococcal enterotoxin genes. exfoliative toxin genes, and toxic shock syndrome toxin I gene. J. Clin. Microbiol. 43: 2548-2553 (1998)   DOI   ScienceOn
44 Bauer AW, Kerdy WM. Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496 (1966)   DOI
45 FDA. Guidance for industry. Guide to minimize microbial food safety hazard for fresh fruits and vegetables. Availahlc From: http://csan.fda.gov. Accessed Oct, 26, 1998
46 Reina LD, Fleming HP, Breidt F. Bacterial contamination of cucumber fruit through adhesion. J. Food Prot. 65: 1881-1887 (2002)   DOI