• Journal of the Optical Society of Korea
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• v.8 no.3
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• pp.132-136
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• 2004

Delayed luminescence of plant leaves was imaged by a 2-D cooled charge-coupled device. We report the delayed luminescence imaging of normal/injured leaves picked ami the leaves intact. The luminescent intensity was lower in leaf veins, scars and edge cut. The intensity of delayed luminescence from intact leaves was higher than that of picked leaves. These results indirectly support the argument that the delayed luminescence of a photosynthetic system is closely related to the electron transfer process of PSII in the thylakoid membrane.

• Reproductive and Developmental Biology
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• v.40 no.3
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• pp.27-31
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• 2016

The aim of this study was to evaluate effect of ${\alpha}$-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at $37.5^{\circ}C$ for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.

• The Korean Journal of Physiology
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• v.12 no.1_2
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• pp.1-5
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• 1978

In an attempt to explore the effect of Ginseng on the permeability of the biological membrane to cations we have investigated the effect of Ginseng-alcohol extract on the transport of $Na^+$ human red blood cell preprations. The $Na^+$ influx was measured in intact red cells using $^{22}Na$ as a tracer and the efflux in reseated red cells using $^{24}Na$ as a tracer. 1. The influx of $Na^+$ was not apparently changed by the Ginseng-alcohol extract of 20mg% in the incubation medium. 2. Similarly, 20mg% Ginseng-alcohol extract in the cellular space did not alter the efflux of $Na^+$ from the cell. However, 50mg% of Ginseng-alcohol extract in the cell resulted in a significant increase in the $Na^+$ efflux and this effect was magnified when the cell was suspended in the medium containing the Ginseng-alcohol extract in a concentration of 20mg %. The results suggest that Ginseng-alcohol extract over 50mg% increase permeability of red blood cell membrane to $Na^+$ by altering the membrane integrity.

• Membrane and Water Treatment
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• v.8 no.6
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• pp.517-528
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• 2017

This study evaluates the performance of membrane bioreactor (MBR) coupled with a modified walnut shell granular activated carbon (WSGAC) for tannery wastewater treatment. For this purpose, a pilot with overall volume of 80L and 12 hours hydraulic retention time (HRT) is operated in three scenarios. Here, the chemical oxidation demand (COD) of wastewater is reduced more than 98% in both C:N ratios of 13 (S1) and 6.5 (S2). This performance also remains intact when alkalinity depletes and pH reduces below 6 (S3). The ammonium removal ranges between 99% (S2) and 70% (S3). The reliability of system in different operating conditions is due to high solids retention time and larger flocs formation in MBR. The average breakthrough periods of WSGAC are determined between 15 minutes (S2) and 25 minutes (S1). In this period, the overall nitrate removal of MBR-WSGAC exceeds 95%. It is also realized that adding no chemicals for alkalinity stabilization and consequently pH reduction of MBR effluent (S3) can slightly lengthen the breakthrough from 15 to 20 minutes. Consequently, MBR can successfully remove the organic content of tannery wastewater even in adverse operational conditions and provide proper influent for WSGAC.

• Reproductive and Developmental Biology
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• v.29 no.4
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• pp.253-257
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• 2005

This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than $80\%$ of fresh sperm washed with mTLP-PVA medium at $20^{\circ}C$ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane $(36.4\~46.9\%)$ and nonfunctional mitochondrion $(55.1\~71.1\%)$ in the mTLP-PVA and BTS washing media at $20^{\circ}C$. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at $4^{\circ}C$ washing temperature than at $20^{\circ}C$ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

• Reproductive and Developmental Biology
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• v.40 no.4
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• pp.33-37
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• 2016

This study was conducted to evaluate effect of ${\alpha}$-linolenic acid (ALA) and bovine serum albumin (BSA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved using freezing extender containing 3 ng/mL ALA and/or $20\;{\mu}g/mL$ BSA. Cryo-preserved boar sperms were thawed in $37^{\circ}C$ water-bath for 45 sec to analysis. Viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed boar sperm was significantly higher in only ALA+BSA supplement group than control group (p<0.05), whereas there was no difference either in ALA or BSA supplement. However, acrosome reacted sperm in both of live and all sperm population were significantly decreased in all treatment groups than control (p<0.05). Interestingly, mitochondrial intact of boar sperm was enhanced in ALA and ALA+BSA groups compared with control (p<0.05). In this study, we showed that supplementation of ALA and BSA in freezing extender enhanced the sperm viability, mitochondrial intact and decrease acrosomal membrane damage. In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by using of ALA and BSA.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.6
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• pp.497-502
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• 2014

Extracellular nicotinamide adenine dinucleotide (NAD) cleaving activity of a particular cell type determines the rate of the degradation of extracellular NAD with formation of metabolites in the vicinity of the plasma membrane, which has important physiological consequences. It is yet to be elucidated whether intact human neutrophils have any extracellular NAD cleaving activity. In this study, with a simple fluorometric assay utilizing $1,N^6$-ethenoadenine dinucleotide (etheno-NAD) as the substrate, we have shown that intact peripheral human neutrophils have scant extracellular etheno-NAD cleaving activity, which is much less than that of mouse bone marrow neutrophils, mouse peripheral neutrophils, human monocytes and lymphocytes. With high performance liquid chromatography (HPLC), we have identified that ADP-ribose (ADPR) is the major extracellular metabolite of NAD degradation by intact human neutrophils. The scant extracellular etheno-NAD cleaving activity is decreased further by N-formyl-methionine-leucine-phenylalanine (fMLP), a chemoattractant for neutrophils. The fMLP-mediated decrease in the extracellular etheno-NAD cleaving activity is reversed by WRW4, a potent FPRL1 antagonist. These findings show that a much less extracellular etheno-NAD cleaving activity of intact human neutrophils compared to other immune cell types is down-regulated by fMLP via a low affinity fMLP receptor FPRL1.

• Membrane Journal
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• v.29 no.2
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• pp.105-110
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• 2019

Porous membranes are widely used in industry for removing particulate matter. Unlike conventional porous membrane fabrication methods, the solution spreading phase separation method can form pores very simply. The first step is to wet the mesh with the support layer, then to let the polysulfone solution flow into a solvent without water. The solvent is readily vaporized and the polysulfone is made into a thin film. When the polysulfone solution is mixed with water to form pores, the pore size can be adjusted according to the concentration ratio of the polysulfone solution. The thickness of the membrane is easily controlled by the concentration of the solution. The porous separator has the formation of meshes intact and is very useful for forming a three-dimensional structure. The solution spreading phase separation method proposed in this study is characterized by its high cost competitiveness compared with conventional membranes due to its low production cost and easy process control.

• Journal of Chest Surgery
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• v.16 no.1
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• pp.34-39
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• 1983

The syndrome of anomalous muscle bundle dividing the right ventricle into two pressure chambers has been described by many authors. The malformation should not be confused with tetralogy of Fallot itself because the obstructive hypertrophic bands are usually proximal to the right ventricular infundibulum. One case [11 years old male] of double chambered right ventricle due to aberrant muscle bundle with intact ventricular septum is presented. The pressure gradient was 68 mmHg between inflow and outflow tracts of right ventricle on cardiac catheterization. On opening the right ventricle, there noted stenosis of outflow tract by infundibular membrane, hypertrophied anomalous muscle bundle, thickened moderator band & hypertrophied anterior papillary muscle. Open heart surgery was carried out with the aid of extracorporeal support, Anomalous muscle bundle [1.0 cm x 4.0 cm] and infundibular membrane were resected safely. The hypertrophied moderator band was cut at mid-portion and anterior papillary muscle was split vertically. The postoperative course was uneventful and discharged in good condition 2 weeks later.

• The Korean Journal of Physiology
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• v.2 no.1
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• pp.39-45
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• 1968

Recently, in this department, pressure-displacement curve and breaking tension of dog and human tympanic membrane were studied using intact, fresh or dried tympanic membrane attached to external auditory meatus. However, physical property, proper elasticity-Young Modulus, of the tympanic membrane has not been clarified yet. Present study is attempted to further clarify proper Young Modulus of tympanic membrane, and to distinguish possible difference between layer of stratum radiatum and layer of stratum circulare of tympanic membrane in breaking tension and in Young Modulus. Tympanic membrane was excised from sacrificed dog, and preparation was made into the size of approximately 1 mm in width and 3 mm in length. In fresh or dried tympanic membrane, which was dried at $80^{\circ}C$ for 24 hrs., some preparations were made along the long axis parallel to the fibers of radial direction, and others were made along the long axis perpendicular to the radial fibers-circular direction. Breaking tension and displacement according to loading, were measured and Young Modulus was calculated in tympanic membrane preparations under the different experimental conditions. Results obtained are summarized as follows : 1. Young Modulus of fresh tympanic membrane in radial direction was $6.57{\times}10^8\;dyne/cm^2$, and that of fresh preparation in circular direction was $1.68{\times}10^8\;dyne/cm^2$. The Young Modulus of fresh tympanic membrane in radial direction resembles to that of silk and whale moustache. In dried tympanic membrane, Young Modulus of preparation of radial direction was $30.2{\times}10^8\;dyne/cm^2$ and that of preparation in circular direction was $25.0{\times}10^8\;dyne/cm^2$. 2. Breaking tension of fresh tympanic membrane was 44.9 gm/mm in radial preparation, and 7.9 gm/mm in circular preparation. In dried tympanic membrane, breaking tension was 46.7 gm/mm in preparation of radial direction, and 17.2 gm/mm in preparation of circular direction. 3. Much smaller breaking tension of the circular preparation-one fifth to the radial preparation-seemed to be responsible for the higher incidence of circular fiber breaking in tympanic membrane performation caused by trauma or sudden change in atmospheric pressure. 4. The correlation seemed to be very close between breaking tension and Young Modulus in tympanic membrane.