Browse > Article
http://dx.doi.org/10.12749/RDB.2016.40.3.27

Effects of α-Linolenic Acid in Frozen-thawed Boar Spermatozoa  

Lee, Won-Hee (College of Animal Life Sciences, Kangwon National University)
Hwangbo, Yong (College of Animal Life Sciences, Kangwon National University)
Lee, Sang-Hee (Institute of Animal Resources, Kangwon National University)
Yang, Jin-Woo (College of Animal Life Sciences, Kangwon National University)
Kim, Hwa-Young (College of Animal Life Sciences, Kangwon National University)
Lee, Yu-Rim (College of Animal Life Sciences, Kangwon National University)
Park, Ji-Eun (College of Animal Life Sciences, Kangwon National University)
Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.40, no.3, 2016 , pp. 27-31 More about this Journal
Abstract
The aim of this study was to evaluate effect of ${\alpha}$-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at $37.5^{\circ}C$ for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.
Keywords
Alpha-linolenic acid; Cryopreservation; Sperm characteristics; Pigs;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Memon AA, Wahid H, Rosnina Y, Goh YM, Ebrahimi M, Nadia FM, Andrey G (2011): Effect of butylated hydroxytoluene on cryopreservation of boer goat semen in Tris egg yolk extender. Anim Reprod Sci 129:44-49.   DOI
2 Aksoy Y, Aksoy H, Altinkayanak K, Aydin HR, Ozkan A (2006): Sperm fatty acid composition in subfertile men. Prostaglandins Leukot Essent Fatty Acids 75:75-79.   DOI
3 Alvarez JG, Storey BT (1995): Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42:334-336.   DOI
4 Bailey JI, Morrie A, Cormier N (2003): Semen cryopreservation: success and persistent problems in farm species. Can J Anim Sci 83(3):393-401.   DOI
5 Bucak MN, Atessahin A, Varisli O, Yuce A, Tekin N, Akcay A (2007): The influence of trehalose, taurine, cystramine and hyaluronan on ram semen: microscopic and oxidative stress parameters after freeze- thawing process. Theriogenology 67(5):1060-1067.   DOI
6 Conquer JA, Martin JB, Tummon I, Watson L, Tekpetey F (1999): Fatty acid analysis of blood, serum, seminal plasma, and spermatozoa of normozoospermic versus asthenozoospermic males. Lipids 34:793-799.   DOI
7 Gholami H, Chamani M, Towhidi A, Fazeli MH (2010): Effect of feeding docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen-thawed semen in Holstein bulls. Theriogenology 74:1548-1558.   DOI
8 O'Flaherty C, Breininger E, Beorlegui N, Beconi MT (2005): Acrosome reaction in bovine spermatozoa: Role of reactive oxygen species and lactate dehydrogenase C4. Biochimica et Biophysica Acta (BBA)-General subjects 1726(1):96-101.   DOI
9 Parks JE, Lynch DV (1992): Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology 29(2):255-266.   DOI
10 Piper PW, Talreja K, Panaretou B, Moradas-Ferreira P, Byrne K, Praekelt UM, Meacock P, Recnacq M, Boucherie H (1994): Induction of major heat-stress proteins of Saccharomyces cerevisiae, including plasma membrane Hsp 30, by ethanol levels above a critical threshold. Microbiology 140(11):3031-3038.   DOI
11 Rooke JA, Shao CC, Speake BK (2001): Effects of feeding tuna oil on the lipid composition of pig spermatozoa and in vitro characteristics of semen. Reproduction 121:315-322.   DOI
12 Samadian F, Towhidi A, Rezayazdi K, Bahreini M (2010): Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm. Animal 4:2017-2022.   DOI
13 Shevchenko A, Simons K (2010): Lipidomics: coming to grip with lipid diversity. Nat Rev Mol Cell Biol 11(8):593-598.   DOI
14 Silva PFN, Gadella BM (2006): Detection of damage in mammalian sperm cells. Theriogenology 65(5):958-978.   DOI
15 Waterhouse KE, Hofmo PO, Tverdal A, Miller Jr RR (2006): Within and breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction 131:887-894.   DOI
16 Watson PF (2000): The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60-61:481-492.   DOI
17 Kaka A, Wahid H, Rosnina Y, Yimer N, Khumran AM, Sarsaifi K, Behan AA, Kaka U, Ebrahimi M (2015b): $\alpha$-Linolenic acid supplementation in BioXcell extender can improve the quality of post-cooling and frozen-thawed bovine sperm. Anim Reprod Sci 153:1-7.   DOI
18 Grobfeld R, Sieg B, Struckmann C, Frenzel A, Maxwell WMC, Rath D (2008): New aspects of boar semen freezing strategies. Theriogenology 70:1225-1233.   DOI
19 Yoshida M (2000): Conservation of sperms: current status and new trends. Anim Reprod Sci 60-61:349-355.   DOI
20 Kaka A, Wahid H, Rosnina Y, Yimer N, Khumran AM, Behan AA, Ebrahimi M (2015a): Alpha-Linolenic acid supplementation in tris extender can improve frozen-thawed bull semen quality. Reprod Dom Anim 50:29-33.   DOI
21 Khoshvaght A, Towhidi A, Zare-shahneh A, Noruozi M, Zhandi M, Davachi ND, Karimi R (2016): Dietary n-3 PUFAs improve fresh and post-thaw semen quality in Holstein bulls via alteration of sperm fatty acid composition. Theriogenology 85:807-812.   DOI
22 Koppers AJ, Garg ML, Aitken RJ (2010): Stimulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa. Free Radical Bio Med 48:112-119.   DOI
23 Lenzi A, Gandini L, Lombardo F, Picardo M, Maresca V, Panfili E (2002): Polyunsaturated fatty acids of germ cell membranes, glutathione and blutathione-dependent enzyme-PHGPx: from basic to clinic. Contraception 65:301-304.   DOI
24 Lessard C, Parent S, Leclerc P, Bailey JI, Sullivan R (2000): Cryopreservation alters the levels of the bull sperm surface protein P25b. J Androl 21:700-707.
25 Maldjian A, Pizzi F, Gliozzi T, Cerolini S, Penny P, Noble R (2005): Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology 63:411-421.   DOI
26 Martinez-Soto JC, Landeras J, Gadea J (2013): Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success. Andrology 1:365-375.   DOI