Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of α-Linolenic Acid in Frozen-thawed Boar Spermatozoa

돼지 정자의 동결보존 시 α-Linolenic Acid의 효과

  • Lee, Won-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Hwangbo, Yong (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (Institute of Animal Resources, Kangwon National University) ;
  • Yang, Jin-Woo (College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Hwa-Young (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Yu-Rim (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Ji-Eun (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 이원희 (강원대학교 동물생명과학대학) ;
  • 황보용 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 공동자원연구소) ;
  • 양진우 (강원대학교 동물생명과학대학) ;
  • 김화영 (강원대학교 동물생명과학대학) ;
  • 이유림 (강원대학교 동물생명과학대학) ;
  • 박지은 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2016.08.05
  • Accepted : 2016.08.29
  • Published : 2016.08.31
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Abstract

The aim of this study was to evaluate effect of ${\alpha}$-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at $37.5^{\circ}C$ for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.

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References

  1. Aksoy Y, Aksoy H, Altinkayanak K, Aydin HR, Ozkan A (2006): Sperm fatty acid composition in subfertile men. Prostaglandins Leukot Essent Fatty Acids 75:75-79. https://doi.org/10.1016/j.plefa.2006.06.002
  2. Alvarez JG, Storey BT (1995): Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42:334-336. https://doi.org/10.1002/mrd.1080420311
  3. Bailey JI, Morrie A, Cormier N (2003): Semen cryopreservation: success and persistent problems in farm species. Can J Anim Sci 83(3):393-401. https://doi.org/10.4141/A03-024
  4. Bucak MN, Atessahin A, Varisli O, Yuce A, Tekin N, Akcay A (2007): The influence of trehalose, taurine, cystramine and hyaluronan on ram semen: microscopic and oxidative stress parameters after freeze- thawing process. Theriogenology 67(5):1060-1067. https://doi.org/10.1016/j.theriogenology.2006.12.004
  5. Conquer JA, Martin JB, Tummon I, Watson L, Tekpetey F (1999): Fatty acid analysis of blood, serum, seminal plasma, and spermatozoa of normozoospermic versus asthenozoospermic males. Lipids 34:793-799. https://doi.org/10.1007/s11745-999-0425-1
  6. Gholami H, Chamani M, Towhidi A, Fazeli MH (2010): Effect of feeding docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen-thawed semen in Holstein bulls. Theriogenology 74:1548-1558. https://doi.org/10.1016/j.theriogenology.2010.06.025
  7. Grobfeld R, Sieg B, Struckmann C, Frenzel A, Maxwell WMC, Rath D (2008): New aspects of boar semen freezing strategies. Theriogenology 70:1225-1233. https://doi.org/10.1016/j.theriogenology.2008.07.017
  8. Kaka A, Wahid H, Rosnina Y, Yimer N, Khumran AM, Behan AA, Ebrahimi M (2015a): Alpha-Linolenic acid supplementation in tris extender can improve frozen-thawed bull semen quality. Reprod Dom Anim 50:29-33. https://doi.org/10.1111/rda.12445
  9. Kaka A, Wahid H, Rosnina Y, Yimer N, Khumran AM, Sarsaifi K, Behan AA, Kaka U, Ebrahimi M (2015b): $\alpha$-Linolenic acid supplementation in BioXcell extender can improve the quality of post-cooling and frozen-thawed bovine sperm. Anim Reprod Sci 153:1-7. https://doi.org/10.1016/j.anireprosci.2014.12.001
  10. Khoshvaght A, Towhidi A, Zare-shahneh A, Noruozi M, Zhandi M, Davachi ND, Karimi R (2016): Dietary n-3 PUFAs improve fresh and post-thaw semen quality in Holstein bulls via alteration of sperm fatty acid composition. Theriogenology 85:807-812. https://doi.org/10.1016/j.theriogenology.2015.10.023
  11. Koppers AJ, Garg ML, Aitken RJ (2010): Stimulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa. Free Radical Bio Med 48:112-119. https://doi.org/10.1016/j.freeradbiomed.2009.10.033
  12. Lenzi A, Gandini L, Lombardo F, Picardo M, Maresca V, Panfili E (2002): Polyunsaturated fatty acids of germ cell membranes, glutathione and blutathione-dependent enzyme-PHGPx: from basic to clinic. Contraception 65:301-304. https://doi.org/10.1016/S0010-7824(02)00276-7
  13. Lessard C, Parent S, Leclerc P, Bailey JI, Sullivan R (2000): Cryopreservation alters the levels of the bull sperm surface protein P25b. J Androl 21:700-707.
  14. Maldjian A, Pizzi F, Gliozzi T, Cerolini S, Penny P, Noble R (2005): Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology 63:411-421. https://doi.org/10.1016/j.theriogenology.2004.09.021
  15. Martinez-Soto JC, Landeras J, Gadea J (2013): Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success. Andrology 1:365-375. https://doi.org/10.1111/j.2047-2927.2012.00040.x
  16. Memon AA, Wahid H, Rosnina Y, Goh YM, Ebrahimi M, Nadia FM, Andrey G (2011): Effect of butylated hydroxytoluene on cryopreservation of boer goat semen in Tris egg yolk extender. Anim Reprod Sci 129:44-49. https://doi.org/10.1016/j.anireprosci.2011.10.004
  17. O'Flaherty C, Breininger E, Beorlegui N, Beconi MT (2005): Acrosome reaction in bovine spermatozoa: Role of reactive oxygen species and lactate dehydrogenase C4. Biochimica et Biophysica Acta (BBA)-General subjects 1726(1):96-101. https://doi.org/10.1016/j.bbagen.2005.07.012
  18. Parks JE, Lynch DV (1992): Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology 29(2):255-266. https://doi.org/10.1016/0011-2240(92)90024-V
  19. Piper PW, Talreja K, Panaretou B, Moradas-Ferreira P, Byrne K, Praekelt UM, Meacock P, Recnacq M, Boucherie H (1994): Induction of major heat-stress proteins of Saccharomyces cerevisiae, including plasma membrane Hsp 30, by ethanol levels above a critical threshold. Microbiology 140(11):3031-3038. https://doi.org/10.1099/13500872-140-11-3031
  20. Rooke JA, Shao CC, Speake BK (2001): Effects of feeding tuna oil on the lipid composition of pig spermatozoa and in vitro characteristics of semen. Reproduction 121:315-322. https://doi.org/10.1530/rep.0.1210315
  21. Samadian F, Towhidi A, Rezayazdi K, Bahreini M (2010): Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm. Animal 4:2017-2022. https://doi.org/10.1017/S1751731110001308
  22. Shevchenko A, Simons K (2010): Lipidomics: coming to grip with lipid diversity. Nat Rev Mol Cell Biol 11(8):593-598. https://doi.org/10.1038/nrm2934
  23. Silva PFN, Gadella BM (2006): Detection of damage in mammalian sperm cells. Theriogenology 65(5):958-978. https://doi.org/10.1016/j.theriogenology.2005.09.010
  24. Waterhouse KE, Hofmo PO, Tverdal A, Miller Jr RR (2006): Within and breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction 131:887-894. https://doi.org/10.1530/rep.1.01049
  25. Watson PF (2000): The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60-61:481-492. https://doi.org/10.1016/S0378-4320(00)00099-3
  26. Yoshida M (2000): Conservation of sperms: current status and new trends. Anim Reprod Sci 60-61:349-355. https://doi.org/10.1016/S0378-4320(00)00125-1