• Title/Summary/Keyword: insecticides.

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Development of Fenvalerate Resistance in the Diamondback Moth, Plutella xylostela Linne (Lepidoptera : Yponomeutidae) and its Cross Resistance (배추좀나방의 Fenvalerate에 대한 저항성 발달과 교차저항성)

  • 김길하;서영식;이준호;조광연
    • Korean journal of applied entomology
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    • v.29 no.3
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    • pp.194-200
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    • 1990
  • The diamondback moth (Plutella xylostella L.) was selected over 24 generations with fenvalerate. The resulting resistant strain was tested to study development of insecticide resistance and cross resistance to some insecticides in the laboratory. Insecticide resistance of diamondback moth at the 24th generation devleoped 66.2 fold compared to the parent strain for fenvalerate. The fenvalerate selected strain exhibited 145 fold, a high level of cross resistance to deltamethrin, and also showed 17.4-45.0 fold cross resistance to alphamethrin, cypermethrin, fenvalerate, permethrin, and tetramethrin in the pyrethroid insecticides. The fenvalerate selected strain showed 2.5-4.3 fold, low cross resistance to diazinon, dichlorvos, EPN, BPMC, cabaryl, and methomyl. However, it did not show cross resistance to acephate, fenitrothion, phenthoate, and carbofuran.

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A Survey on the Actual Condition related to Clothing Custody and Managerial Behaviors of Elementary School Senior Students (초등학교 고학년의 의복보관과 관리행동에 관한 실태조사)

  • Cho, Young-Ok;Jung, Ji-Yoon;Hwang, Yeon-Soon
    • Fashion & Textile Research Journal
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    • v.13 no.1
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    • pp.100-106
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    • 2011
  • The primary purpose of this study was to research on the actual condition related to clothing custody and managerial behaviors of elementary school senior students. The data were collected from 296 elementary school senior students in Pusan. The aforementioned were analyzed utilizing frequency, factor analysis, ANOVA, t-test using SPSS Win 12.0. The results were as follows. First, factor analysis on clothing custody identified two groups such as classified custody by uses and season, and custody used insecticides and desiccating agents. Second, in the classify by uses and season case, there were significant differences economic level, purchasing place, dealing method of laundry, discriminating ability whether or not laundry, arrangement frequency, basic sewing ability, holdings and disposal. Third, in the use of insecticides and desiccating agents case, there were significant differences purchasing place, basic sewing ability, holdings and disposal.

Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control Potential of Essential Oil Isolated from Iranian Cymbopogon citratus Stapf

  • Ebadollahi, Asgar;Geranmayeh, Jafar;Kamrani, Morteza
    • Natural Product Sciences
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    • v.23 no.4
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    • pp.235-238
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    • 2017
  • Colorado potato beetle is a most destructive insect pest of potato throughout the world. Although utilization of chemical insecticides is a main method for management of this pest, their negative side-effects such as threat to humans and the environmental pollution prompted researchers to search for natural alternatives. Recently plant essential oils with low or without side-effects against noun-targeted organisms and with high availability were considered as safe bio-pesticides. In the present study, toxicity of essential oil of Iranian lemongrass, Cymbopogon citratus Stapf, was evaluated against 3th instar larvae and adults of Colorado potato beetle by a leaf dipping method. Results displayed essential oil had notable toxicity against both larvae and adults after 24 and 48 h exposure times. Probit analysis revealed $LC_{50}$ values (lethal concentration to kill 50% of population) with 95% confidence limits were 10.32 (9.17 - 11.72) and 7.76 (6.80 - 8.74) ${\mu}l/ml$ for larvae and 6.27 (4.82 - 8.15) and 4.35 (3.24 - 5.62) ${\mu}l/ml$ for adults after 24 and 48 h, respectively. Based on regression analysis, a positive correlation between log concentration of essential oil and insect mortality was achieved. Results indicated C. citratus essential oil can be candidate as a natural alternative to the harmful chemical insecticides in the management of Colorado potato beetle.

Expression of Mosquitocidal Bacillus sphaericus Binary Toxin and B. thuringiensis cry11B Genes in B. thuringiensis 407

  • Park, Hyun-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.185-189
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    • 2001
  • Wild type Bacilus thuringiensis subsp. israelensis and B. sphaericus toxins have been used separately as active in ingredients for bacterial insecticides to control mosquito larvae due to their comparable toxicity to chemical insecticides. Cry11B, recently cloned from B. thuringiensis subsp. jegathesan, shows higher toxicity against three major species of mosquito larvae than Cry11A, one of the major component of B. thuringiensis subsp. israelensis inclusion body. To determine whether the combination of cry11B and B. sphaericus binary toxins is as toxic as B. thuringiensis subsp. israelensis parental strain, cry11B and B. sphaericus binary toxins genes were co-expressed as an operon using cytlA promoters/STAB-SD hybrid expression system in B. thuringiensis subsp. israelensis acrystalliferous strain 4Q7. However, unexpectedly, B. sphaericus binary toxins were barely produced, whereas relatively large amount of Cry11B was produced. When this strain was grown in four different media, NB+G and Peptonized Milk produced more toxin proteins and spores per unit of media than GYS and G-Tris. Toxicity of this strain against fourth instar Culex quinquefasciatus was ranged from of 8.3 to 45.7 ng/ml, with NB+G culture being the highest, and GYS culture was the lowest.

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Occurrence of Organochlorine Insecticides in Vegetable Oils Produced in Korea (한국산 식물성(植物性) 기름 중 유기염소계(有機墮素系) 살충제(殺童劑)의 잔류량(殘留量))

  • Lee, Su-Rae;Kang, Soon-Young;Kim, Yong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.12 no.3
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    • pp.216-218
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    • 1980
  • The appearance of organochlorine insecticides in 43 samples of refined vegetable oils including rapeseed, rice bran, sesame, perilla and corn oils produced in Korea in 1976 was investigated. Residue levels varied depending on the pesticide, oil source and production area. Gas-liquid chromatographic techniques were used to detect and quantify the presence of heptachlor, its epoxide, BHC, aldrin, endrin and DDT residues.

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DNA Damage Effect of Botanical Insecticides Using Chinese Hamster Lung Cells

  • Kim, Areumnuri;Jeong, Mihye;Park, Kyung-Hun;Chon, Kyongmi;Cho, Namjun;Paik, Min Kyoung
    • Korean Journal of Environmental Agriculture
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    • v.34 no.4
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    • pp.350-354
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    • 2015
  • BACKGROUND: Botanical insecticides, especially Azadirachta Indica extract (AIE) and Sophorae radix extract (SRE) are widely used in Agriculture field. In our previous studies on genotoxicity test of AIE and SRE samples, a suspicious clastogenic properties was shown. Herein, we investigated the DNA damage effect of these botanical insecticide samples through the in vitro comet assay. METHODS AND RESULTS: Chinese hamster lung (CHL) fibroblast cell line was used, and methyl methanesulphonate was as positive control. Respective two samples of AIE and SRE were evaluated using Single Cell Gel Electrophoresis (Comet) assay and measured as the Olive tail moment (OTM). Results from this study indicated that all tested AIE and SRE samples did not show DNA damage in comet assay using CHL cells, compared with control. CONCLUSION: AIE and SRE samples used in this study were not cause genetic toxicity and are suitable for use as organic materials.

The effects of several insecticides on the control of the pear stem sawfly, Janus piri Okamoto et Muramatsu (배나무 줄기벌에 대한 몇 가지 살충제의 방제효과)

  • Yun Ju Kyung
    • Korean journal of applied entomology
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    • v.7
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    • pp.53-55
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    • 1969
  • At Nowan Myun and Bia Myun of Cholla-namdo the pear stem sawflies were found as the most serious pests of the pear trees. The writer attempted to control the pear stem sawfly by spraying certain insecieides in 1968. 1) The significance of each chemical applicated turned out to be as shown in Table 2 and 3. The priority of effectiveness among the chemicals turned out to be as follows: Dimecron>Lebaycid>D. S. >Endrin>Metasystox>DDVP 2) It was also found that the significance vary according to the difference in time of application of the insecticides as shown in Table 2 and 3. Dimecron and Lebaycid were found to be most effective at the hatching day and the 4th day after the hatching.

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A Case of Chlorfluazuron Insectisides Poisoning with Mental Change (클로르플루아주론 살충제 음독 후 발생한 의식변화 1례)

  • Park, Eun Suk;Kang, Soo;Kim, Ah Jin;Baek, Jin Hue;Jung, Hyun Min
    • Journal of The Korean Society of Clinical Toxicology
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    • v.13 no.1
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    • pp.40-42
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    • 2015
  • Benzoylureas are chemical compounds best known for their use as insecticides. Diflubenzuron is one of the more commonly used benzoylurea pesticides. Others include chlorfluazuron, flufenoxuron, hexaflumuron, and triflumuron. They act as insect growth regulators by inhibiting synthesis of chitin in the body of the insect. They have low toxicity in mammals because mammals have no chitin. Chlorfluazuron insecticides, which are mixed with solvent naphatha, are commonly used. Thus we assume that in the presented case mental change outcome of poisoning was connected with toxic effects of solvent naphtha rather than with chlorfluazuron action. Components of solvent naphtha, particularly trimethylbenzenes, exert strong irritant action on the gastric mucosa and are very well absorbed from the gastrointestinal tract. We report on a 67-year-old man with stuporous mentality after intentional ingestion of approximately 200 ml of liquid chlorfluazuron in a suicide attempt. He was discharged after conservative treatments including gastric irrigation, charcoal, mechanical ventilation, hydration, and antibiotics for aspiration pneumonia without complications.

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Study on the development of simultaneous-rapid screening methods for residues pesticides in livestock products (수입 축산식품에서의 잔류농약 동시분석법 개발에 관한 연구)

  • Kim, Jong-shu;Kim, Gon-sup;Hah, Dae-sik
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.213-220
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    • 1997
  • A simple and rapid method known as matrix solid phase dispersion(MSPD) for simultaneous determination of 11 pesticide(2,4,5,6-tetrachlor m-xylene, ${\alpha}$-BHC, ${\gamma}$-BHC, ${\delta}$-BHC, aldrin, chlorfulazuron, heptachloroepoxide, dieldrine, endrin, endosulfan sulfate, and tetradifon) in beef fat was estabilished. Beef fat(0.5g) was fortified by adding the 11 pesticides and dibutylchlorendate as internal standard, and blended with 2g bulk $C_{18}$ in pestle and motar. Pesticides were eluted from an extraction column composed of $C_{18}$/ fat matrix blend and 2g activated Florisil by addition of 8ml acetonitrile. Then $2{\mu}l$ portion of the acetonitrile elute was directly analyzed by gaschromatography with electron capture detection. Unfortified blank control were treated similarly. Recovery rate were ranged from $83{\pm}5.4%$ to $94.2{\pm}7.6%$, intra-assay variability and inter-assay variability were ranged from 2.3% to 7.4%(n=5 for each insecticides) and from $6{\pm}1%$ to $12{\pm}3%$(n=10 for each insecticides), respectively. These results indicated that the MSPD methodology is aceptable for the extraction, determination and screening of residues 11 chroniated pesticides in beef fat.

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Insecticide Targets: Learning to Keep Up with Resistance and Changing Concepts of Safety

  • Casida, John E.;Quistad, Gary B.
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.185-191
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    • 2000
  • Pest insect control is dependent on about 200 insecticides that work by relatively few mechanisms. The targets they disrupt are mostly involved in the nervous system, respiratory chain, growth and development, or the gut. The major nerve targets are: acetylcholinesterase for the organophosphates and methylcarbamates; the nicotinic acetylcholine receptor for the neonicotinoids; the $\gamma$-aminobutyric acid receptor for several chlorinated hydrocarbons and fipronil; the voltage-gated sodium channel for DDT and pyrethroids. Selection of resistant strains often confers cross-resistance to some or all other insecticides working at the same site. The toxicological properties of different compounds acting on the same target are increasingly considered together, summating the risk even though the compounds are of quite diverse chemical types. Continuing attention is also being given to secondary targets not involved in the primary mechanism of toxicity but instead in side effects that must be considered in the overall safety evaluation. Research on insecticide targets is important in learning to keep up with resistance and changing concepts and policies on safety. These relationships are illustrated by recent studies in the Environmental Chemistry and Toxicology Laboratory of the University of California at Berkeley.

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