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http://dx.doi.org/10.5338/KJEA.2015.34.4.40

DNA Damage Effect of Botanical Insecticides Using Chinese Hamster Lung Cells  

Kim, Areumnuri (Chemical Safety Division, National Institute of Agricultural Sciences)
Jeong, Mihye (Research Policy Bureau, Rural Development Administration)
Park, Kyung-Hun (Chemical Safety Division, National Institute of Agricultural Sciences)
Chon, Kyongmi (Chemical Safety Division, National Institute of Agricultural Sciences)
Cho, Namjun (Chemical Safety Division, National Institute of Agricultural Sciences)
Paik, Min Kyoung (Chemical Safety Division, National Institute of Agricultural Sciences)
Publication Information
Korean Journal of Environmental Agriculture / v.34, no.4, 2015 , pp. 350-354 More about this Journal
Abstract
BACKGROUND: Botanical insecticides, especially Azadirachta Indica extract (AIE) and Sophorae radix extract (SRE) are widely used in Agriculture field. In our previous studies on genotoxicity test of AIE and SRE samples, a suspicious clastogenic properties was shown. Herein, we investigated the DNA damage effect of these botanical insecticide samples through the in vitro comet assay. METHODS AND RESULTS: Chinese hamster lung (CHL) fibroblast cell line was used, and methyl methanesulphonate was as positive control. Respective two samples of AIE and SRE were evaluated using Single Cell Gel Electrophoresis (Comet) assay and measured as the Olive tail moment (OTM). Results from this study indicated that all tested AIE and SRE samples did not show DNA damage in comet assay using CHL cells, compared with control. CONCLUSION: AIE and SRE samples used in this study were not cause genetic toxicity and are suitable for use as organic materials.
Keywords
Botanical insecticide; Comet assay; DNA damage;
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Times Cited By KSCI : 2  (Citation Analysis)
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