• Genomics & Informatics
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• 제10권3호
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• pp.206-211
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• 2012

DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59 shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.

• 한국균학회지
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• 제37권2호
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• pp.139-143
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• 2009

Several entomopathogenic fungi have been exploited to be developed into biological control agents in insect pest management. The beet armyworm, Spodoptera exigua, is a serious insect pest infesting various crops, but not effectively controlled by commercial chemical pesticides due to its high insecticide resistance. A fungal isolate was isolated from S. exigua larvae collected from cabbage field in Andong, Korea. The fungus could be cultured in potato dextrose agar. Larvae of S. exigua injected with the cultured conidia showed a potent entomopathogenicity. To identify the fungus isolate, its internal transcribed space (ITS) and surrounding partial 18S/28S regions were sequenced. The ITS sequence was highly matched (99%) to that of Nomuraea rileyi. Morphological characters of its hyphae and conidia were well fit to those of known N. rileyi. This study reports the first record of an entomopathogenic fungus, N. rileyi, in Korea.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.131-136
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• 2005

Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a pr1-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the pr1-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, pr1 genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111 bp including 52 bp intron. The deduced Pr1-like peptide showed a low identity with Pr1s of entomopathogenic fungi such as B. bassiana Pr1 (BbPr1) and M. anisopliae Pr1 (MaPr1) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity $(>65\%)$ with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Pr1-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.

• 한국균학회지
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• 제48권1호
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• pp.69-74
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• 2020

2017년 9월 춘천의 오봉산에서 참싸리 잎에 겹둥근무늬가 생기고 조기낙엽이 일어나는 등 큰 피해가 발견되었다. 병든 잎의 뒷면에는 원뿔형의 흰색 포자과가 다수 발견되었다. 2019년 9월 횡성의 태기산에서도 참싸리 잎에서 유사한 증상이 발견되었다. 이러한 증상에서 발견된 포자과의 형태적 특징으로 보아 이 곰팡이는 Grovesinia moricola와 일치하였다. 확보된 두 균주(KACC48417 및 KACC48934)의 ITS rDNA 염기서열 분석을 통해 동정을 확인하였다. 이 곰팡이의 병원성은 상대습도 100%와 15±2℃의 조건에서 인공접종을 통하여 확인하였다. 이는 한국에서 참싸리와 G. moricola의 관계를 연구한 최초의 보고이다.

• 한국균학회지
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• 제46권1호
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• pp.69-74
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• 2018

진달래속의 흰가루병균인 Erysiphe izuensis는 중국, 일본, 한국, 러시아 등 아시아에서만 기록되어있다. 한국에서는 무성세대의 형태적 특징에 의해, 도입된 진달래속 식물에서 E. izuensis가 기록되었다. 최근에 저자들은 산철쭉에서 흰가루병균을 국내에서 처음으로 채집하였다. 본 연구를 통하여 국내에서 E. izuensis의 유성세대의 형태적 특징과 internal transcribed spacer 염기서열을 처음으로 제공하였다. 게다가, 이 종에서 그 동안 알려지지 않았던 1차 분생포자와 분생포자의 표면구조 등 무성세대의 형태적 특징을 처음으로 기재하였다.

• 한국응용곤충학회지
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• 제59권2호
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• pp.117-127
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• 2020

2018년 6월 1일부터 9월 17일까지 109일 동안, 총 112개 국외 발 국내 입항 선박을 대상으로 편승자 해충(hitchhiker insect pests)에 대한 모니터링을 실시하였다. 그 결과, 총 336개체의 편승자 해충을 확보하였으며, 이를 통합 분류학적 종동정 방법(integrative identification method)을 이용하여 9목 47과 159종으로 동정하였다. 이 중 총 3목 9과 14종의 한국 미분포종(not-distributed species)이 확인되어 보고하며, 검출된 미분포종에 대해서 침입종(invasive species) 내지 침입 가능종(invasive likelihood species)에 대한 위험성 평가를 위한 data sheet를 제공하였다. 또한, 미기록(unrecorded species), 미보고종(unreported species) 내지 신기록종(new recorded species)과 용어의 혼동을 피하기 위하여 본 연구에서는 미분포종(not-distributed species)을 제안하여 사용하였다.

• 한국응용곤충학회지
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• 제60권3호
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• pp.269-276
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• 2021

2018년 7월 13일부터 2019년 10월 29일까지 1년 3개월 동안, 총 306개 국외 발 국내 입항 선박을 대상으로 한국 미분포 편승자 해충(not-distributed hitchhiker insect pests in Korea)에 대한 모니터링을 실시하였다. 그 결과, 총 805개체의 편승자 해충을 확보하였으며, 이를 통합 분류학적 종동정 방법(integrative identification method)을 이용하여 총 12목 78과 379종으로 동정하였다. 이 중 7목 21과 42종 67개체의 한국 미분포종이 확인되었는데, 10종이 다중검출된 것으로 나타났으며, 그 중 7종은 2018년도에 이어 2019년도에도 검출된 것으로 확인되었다. 또한, 농림축산검역본부에 관리해충으로 등재되어 있는 Erthesina fullo (Pentatomidae, Hemiptera)와 Tessaratoma papilosa (Tessaratomidae, Hemiptera)가 검출되었다. 이에 따라, 검출된 미분포종의 사전 조사 및 모니터링 방안 마련뿐만 아니라 침입종(invasive species) 내지 침입 가능종(invasive likelihood species)에 대한 위험성 평가를 위한 data sheet를 제공하였다.

• Journal of Ecology and Environment
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• 제47권4호
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• pp.250-260
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• 2023

Background: Crabgrass (Digitaria ciliaris, Poaceae) is a globally distributed weed, including in Afro-Eurasia, America, and Australia. As a highly gregarious plant, crabgrass is an important habitat for a diverse array of insects, and a potential habitat for agricultural pests. To compare the insect communities associated with the crabgrass community, insects were sampled using sweep sampling (100 sweeps per sample) at five sites, including Daejeon (Daejeon and Gap rivers), Anseong, Namhae, and Inje, with a focus on the Daejeon River. Results: A total of 5,888 individual insects belonging to eight orders, 42 families, and 115 species were collected from the five sites. Both the number of species and individuals of Hemiptera were the highest at all of the sites. In the present study, 73% of the insect population fed on D. ciliaris as a host plant. The dominant species in the D. ciliaris community was Laodelphax striatellus (Delphacidae), being ubiquitous at all the sites which showed a high abundance of rice pests in the communities and the suitability of D. ciliaris as an alternative host plant for them. The Shannon-Wiener diversity index was highest in Inje on 17 September (2.88), and the Chao1-bc diversity index was highest in the Gap River on 5 September (80). The sampling efficiency of 100 sweep samples (sample coverage) was calculated to be as high as 90%. The results of the samples taken from September to November in the Daejeon River showed that the number of species and individuals decreased gradually over time, and the number of dominant species decreased sharply between September and October. Similarity analysis indicated that sampling dates that were closer together yielded sampled assemblages with higher faunal similarity. In addition, in each sampling, the difference in the minimum temperature during the two-week period prior to sampling and faunal similarities were negatively correlated. Conclusions: This study provides foundational data that could enhance our understanding of insect diversity in D. ciliaris. The data can facilitate ecological conservation and management of Korean grasslands generally, as well as identification of potential pests that may disperse from D. ciliaris communities to nearby farmland.

• Journal of Microbiology and Biotechnology
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• 제29권6호
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• pp.887-896
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• 2019

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based pathogen identification relies on the ribosomal protein spectra provided in the proprietary database. Although these mass spectra can discern various pathogens at species level, the spectra-based method still has limitations in identifying closely-related microbial species. In this study, to overcome the limits of the current MALDI-TOF MS identification method using ribosomal protein spectra, we applied MALDI-TOF MS of low-mass profiling to the identification of two genetically related Bacillus species, the food-borne pathogen Bacillus cereus, and the insect pathogen Bacillus thuringiensis. The mass spectra of small molecules from 17 type strains of two bacilli were compared to the morphological, biochemical, and genetic identification methods of pathogens. The specific mass peaks in the low-mass range (m/z 500-3,000) successfully identified various closely-related strains belonging to these two reference species. The intensity profiles of the MALDI-TOF mass spectra clearly revealed the differences between the two genetically-related species at strain level. We suggest that small molecules with low molecular weight, 714.2 and 906.5 m/z can be potential mass biomarkers used for reliable identification of B. cereus and B. thuringiensis.