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Identification of Entomopathogenic Fungus, Beauveria spp. F-101 Isolated from Thecodiplosis japonensis Using Internal Transcribed Spacer Sequence  

Shin, Sang-Chul (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute)
Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University)
Kim, Chul-Su (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute)
Park, Il-Kwon (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute)
Jeon, Mun-Jang (Department of Life & Environment, College of Natural Resources, Taegu University)
Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.8, no.1, 2004 , pp. 77-80 More about this Journal
Abstract
For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.
Keywords
Beauveria bassiana F-l01; Pathogenicity; Thecodiplosis japonensis; Acantholyda parki; Internal transcribed spacer; Forest pest control;
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