• 한국식품영양과학회지
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• 제39권7호
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• pp.1083-1086
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• 2010

본 연구에서는 대두 isoflavone의 하나인 daidzein의 콜레스테롤 당전이 효소에 대한 저해효과와 헬리코박터에 대한 항균력을 측정하였다. 콜레스테롤 당전이 효소는 헬리코박터 파이로리의 세포벽 지질성분을 구성하는 $\alpha$-glucosyl cholesterol을 생성하는 데 관여하는 효소로 헬리코박터의 생육에 있어서 중요한 역할을 하고 있다. Daidzein을 농도별로 처리하였을 경우, 콜레스테롤 당전이 효소의 활성은 daidzein의 농도에 의존적으로 저해를 나타내었으며, daidzein의 $IC_{50}$값은 $128.5\;{\mu}M$이었다. 또한 disc diffusion 방법을 이용하여 항균실험에 있어서도 daidzein은 헬리코박터의 생육을 억제하였다. 이상의 결과를 통하여 daidzein의 효소저해 활성은 헬리코박터의 생육 저해와 밀접한 연관이 있는 것으로 판단된다.

• Applied Biological Chemistry
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• 제51권1호
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• pp.84-87
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• 2008

Streptococcus mutans, Streptococcus sobrinus 등과 같은 구강 세균에 의해 생산되는 효소인 glucosyltransferase(GTase; sucrose 6-glucosyltransferase, EC 2.4.1.5) 는 충치 발생에 중요한 역할을 하는 것으로 알려져 있으며 Gtase-I, GTase-SI, GTase-S 등 최소 3종의 isozyme이 알려져 있다.$^{7-10)}$ GTase는 sucrose로부터 점착성이 있는 불용성의 glucan을 형성하고 이 glucan에서 구강 미생물이 증식하며 그 결과로 생성된 유기산에 의하여 치아표면의 에나벨 층이 부식되므로써 충치가 유발되게 된다.$^{11)}$ 따라서 불용성 glucan의 생성을 유도하는 GTase의 활성을 조절하는 것은 충치 발생 억제에 중요한 역할을 하는 것으로 판단된다. 본 연구자 등은 GTase를 효율적으로 저해하여 충치를 예방할 물질을 탐색하기 위한 연구의 일환으로 약 420종 식물체의 ethanol 추출물로부터 GTase 저해 활성을 검색하여 보고한 바 있다. 수종의 식물체 ethanol 추출물은 S. mutans에 대한 정균활성과 GTase 저해 활성을 동시에 나타내었으며 물질의 분리${\cdot}$정제로부터 oleic acid를 주성분으로 히는 불포화 지방산임이 밝혀진바 있다.$^{12)}$ 본 연구에서는 약 420종 식물체의 열수추출물을 대상으로 GTase 저해 활성을 검색하였다. 그 중 13종의 열수 추출물에서 비교적 높은 GTase 저해활성이 분포 되고 있음을 확인하였으며 활성이 높은 회향을 대상 식물체로 하였다. 회향의 열수 추출물은 S. mutans의 생육에는 영향을 미치지 않고 GTase 활성을 저해하는 특정을 가지고 있다(data not shown). 특히 사람을 대상으로 한 회향 열수 추출물에 의한 치면 세균막 억제 임상 실험 결과 plaque index의 저하를 가져오는 것으로 나타나 회향 열수 추출물은 GTase 활성을 저해하여 충치 예방 목적으로 적용 할 수 있음을 알 수 있다. 회향은 한국을 비롯한 아시아권에서 식품의 향신료 및 민간요법에 널리 사용되는 식물이고 식품의 원료로 사용할 수 있는 소재이다. 회향의 열수 추출물은 열에 대해 높은 안정성을 나타내는 가공 특성을 가지고 있으므로 천연물 기원의 충치예방 기능성 식품으로서의 산업적 이용가치가 기대된다.

• 동의생리병리학회지
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• 제21권3호
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• pp.647-651
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• 2007

It has been well established that S. mutans is the major etiological agent in dental caries, one of the most common oral diseases worldwide. The present study was designed to investigate the effect of Saururus chinensis (S. chinensis) ethanol extracts on the growth, acid production, biofilm formation, adhesion, and insoluble glucan synthesis of S. mutans. The ethanol extracts of S. chinensis showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.025, 0.05, 0.1, 0.2 and 0.4 mg/ml compared to the control group. The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 80% at the concentration of 0.05 mg/ml and complete inhibition was observed at the concentration of 0.4 mg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan form sucrose, ethanol extract of S. chinensis showed more than 10% inhibition over the concentration of 0.025 mg/ml. The synthesis of insoluble glucan was decreased in the presence of 0.025 ${\sim}$ 0.4 mg/ml of the ethanol extract of S. chinensis. Our research strongly suggested S. chinensis was a promising natural product for the prevention of dental caries.

• 동의생리병리학회지
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• 제19권2호
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• pp.370-374
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• 2005

Streptococcus mutans (S. mutans) is known as the causative bacterial playing the most important role informing plaque and it is being noticed as major causative bacteria of dental caries. Therefore, development of more effective, substantial and safe preventive agent against dental caries and periodontal disease is honestly required. The present study was designed to investigate the effect of Cyperus rotundus (Cyperaceae) methanol extracts on the growth, acid production, adhesion, and insoluble glucan synthesis of S. mutans. The methanol extract of C. rotundus showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.5, 1, 2 and 4 mg/ml compared to the control group. The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 50% at the concentration of 0.5 mg/ml and complete inhibition was observed at the concentration of 4 mg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan from sucrose, methanol extract of C. rotundus showed more than 10% inhibition over the concentration of 2 mg/ml. Thus, the application of C. rotundus can be considered a useful and a practical method for the prevention of dental caries.

• 동의생리병리학회지
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• 제21권5호
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• pp.1148-1153
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• 2007

Streptococcus mutans is considered one of the primary etiologic agents of dental caries. we studied the effect of the ethanol extracts of Tribuli fructus (T. fructus) on the growth, biofilm formation, acid production, adhesion and insoluble glucan synthesis of S. mutans. The ethanol extracts of T. fructus showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.025, 0.05, 0.1, 0.2, 0.3, 0.5 mg/ml compared to the control group. In the biofilm assay, the ethanol extracts of T. fructus inhibited formation of biofilm synthesized by S. mutans at the concentration of 0.05 mg/ml. The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 50% at the concentration 0.05 mg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan form sucrose, ethanol extract of T. fructus showed more than 10% inhibition over the concentration of 0.025 mg/ml. Hence, we conclude that T. fructus might be a candidate of anticaries agent. Further studies are necessary to clarify the active constituents of T. fructus responsible for such biomolecular activities.

• 한국식품과학회지
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• 제32권1호
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• pp.168-173
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• 2000

애사과 polyphenol의 충치예방효과를 확인하고자, S. mutans 4 균주 가운데 GTase 활성이 가장 높은 S. mutans MT 8148 균주에 대하여 애사과 polyphenol의 GTase 활성억제효과, cell adherence 억제효과, 산 생성억제효과를 측정하였다. S. mutans GTase에 대한 활성 억제효과는 애사과 polyphenol 성분 중 (+)-catechin과 tannic acid가 1 mg/ml의 농도에서 60% 이상, 5mg/ml의 농도에서 90% 이상으로 높게 나타났다. S. mutans의 adherence에 대해서는 tannic acid가 약 50% 정도의 억제효과를 나타냈으며, 애사과 추출물도 약 30%정도의 억제효과를 보였다. 그러나 S. mutans의 산 생성에 대해서는 애사과 polyphenol 성분들이 억제효과를 나타내지 못하는 것으로 나타났다. 애사과 polyphenol 성분들의 S. mutans에 대한 항균활성을 확인한 결과, 이들은 항균활성은 갖지 않는 것으로 나타났다. 애사과 polyphenol의 충치억제효과는 주성분중의 하나인 catechin과 tannic acid의 S. mutans GTase 활성억제와 cell adherence 억제효과로부터 기인된 것임을 알 수 있었다. 따라서 애사과 추출물은 충치예방을 위한 기능성 식품소재로 사용 가능할 것으로 생각된다.

• 동의생리병리학회지
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• 제19권1호
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• pp.87-91
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• 2005

Dental plaque is a film of microorganisms on the tooth surface that plays an important part in the development of caries and periodontal diseases. Streptococcus mutans (S. mutans) is present in almost all types of dental plaque. Teeth and their supporting structure, the gums (gingiva) are subjected to infection by S. mutans that causes cavities and pyorrhea which, if left untreated, can eventually lead to gingivitis. Various chemical agents have been evaluated over the years with respect to their antimicrobial effects in the oral cavity; however, all are associated with side effects that prohibit regular long-term use. The present study was designed to investigate the effect of Aralia continentalis (Araliaceae) extracts on the growth, acid production, adhesion, and insoluble glucan synthesis of S. mutans. The methanol extract of A. continentalis showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.25, 0.5, 1, 2 and 4 mg/ml compared to the control group. The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 60% at the concentration of 0.25 mg/ml and complete inhibition was observed at the concentration of 4 mg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan from sucrose, methanol extract of A. continentalis showed more than 10% inhibition over the concentration of 0.25 mg/ml. The synthesis of insoluble glucan was decreased in the presence of 0.25 - 4 mg/ml of the methanol extract of A. continentalis. Hence, we conclude that A. continentalis might be a candidate of anticaries agent.

• 대한미생물학회지
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• 제17권1호
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• pp.75-85
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• 1982

In order to demonstrate the effect of specific serum IgG antibody on the adherence of Streptococcus mutans to smooth surface and the mechanism of effective adherence inhibition by IgG antibody, in the present study authors obtained purified IgG from different immunogen preparations of S. mutans NCTC 10449(serotype c) and observed the effect of each IgG preparation on the adherence of each S. mutans strain cultured in different conditions. In addition, the present study was undertaken to observe the cross-reactivity of IgG and the effect of sucrose concentration on the adherence of S. mutans in vitro non-growth condition. The adherence of S. mutans to glass surface was effectively inhibited by serum IgG antibody. At the same IgG concentrations, anti-2% fructose grown/1N NaCl washed S. mutans NCTC 10449 cell showed greater adherence inhibitory effect to S. mutans strains than anti-2% sucrose grown and anti-S. mutans NCTC 10449 cell wall, and the greater inhibitory effects of IgG preparations were observed in assay using 2% fructose grown S. mutans cell preparations than using 0.1% sucrose grown cell preparations. These results suggest that the more effective adherence inhibition by serum IgG antibody is due to the reaction with S. mutans cell surface antigens rather than glucan and cell-associated glucosyltransferase. The greatest adherence inhibitory effect of IgG to S. mutans strains was observed on homologous NCTC 10449 strain and the inhibition cross-reactivities were observed between serotype c, e, and f strains. More pronounced cross-reactivity of adherence inhibition of IgG to S. mutans was observed in assay using anti-2% fructose grown/1N NaCl washed cell than using other IgG preparations, and observed in assay using 2% fructose grown S. mutans cell preparations than 0.1% sucrose grown cell preparations. It was interested that low, but adequate concentration of reactive IgG antibody significantly increased the adherence ability of S. mutans. This result may be due to the formation of small cell aggregates resulted in a increase in the numbers of organisms which adhered to glass surface. The adherence of S. mutans to glass surface was possible in the absence of glucan-synthetic activity. Low level of sucrose significantly increased the adherence ability of S. mutans to glass surface, but excessive amount of sucrose induced large cell aggregates resulted in a decrease in the numbers of organism which adhered.

• 동의생리병리학회지
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• 제29권1호
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• pp.27-32
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• 2015

Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum ) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.

• 동의생리병리학회지
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• 제30권1호
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• pp.27-32
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• 2016

Streptococcus mutans plays a virtal role in trigering dental caries establishment due to its ability to synthesize two significant factors. The two factors are organic acids and glucans. The former demineralized dental enamel and the latter mediates the attachment of bacteria to tooth surface. It is believed that demineralization of dental enamel and attachment of bacteria are the crucial events that indicate and develop dental caries. For this reason, we studied the effect of the ethanol extracts of Radix Pulsatillae on the growth and acid production of S. mutans. Ethanol extracts of the Radix Pulsatillae showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by Radix Pulsatillae. the ethanol extract of Radix Pulsatillae showed remarkable inhibition of glucosyltransferase, which synthesizes water insoluble glucan form sucrose. Phytochemical analysis showed Radix Pulsatillae contained major components such as phenolic compounds, glycosides, steroids, terpenoid, and saponin. These results suggest that Radix Pulsatillae may have anti-cariogenic properties, which may be related with major components such as phenolic compounds, glycosides, steroids, terpenoid, and saponin.