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http://dx.doi.org/10.15188/kjopp.2016.02.30.1.27

Inhibitory Effects of Radix Pulsatillae Extract on Insoluble Glucan Synthesis and Adhesion of Streptococcus mutans  

Kim, Kang Ju (Department of Oral Microbiology of Wonkwang University)
Park, Bog Im (Department of Oral Biochemistry of Wonkwang University)
Min, Jae Hong (Department of Oral Biochemistry of Wonkwang University)
Chae, Min Suk (Department of Oral Biochemistry of Wonkwang University)
Lim, Jae You (Department of Oral Biochemistry of Wonkwang University)
Son, Hyeok Jin (Department of Oral Biochemistry of Wonkwang University)
Lee, Gi Hoon (Department of Oral Biochemistry of Wonkwang University)
An, So Youn (Department of Pediatric dentistry, School of Dentistry of Wonkwang University)
Jeon, Byung Hun (Department of Pathology, College of Korean Medicine of Wonkwang University)
Choi, Na Young (Department of Home Economics Education of Wonkwang University)
You, Yong Ouk (Department of Oral Microbiology of Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.1, 2016 , pp. 27-32 More about this Journal
Abstract
Streptococcus mutans plays a virtal role in trigering dental caries establishment due to its ability to synthesize two significant factors. The two factors are organic acids and glucans. The former demineralized dental enamel and the latter mediates the attachment of bacteria to tooth surface. It is believed that demineralization of dental enamel and attachment of bacteria are the crucial events that indicate and develop dental caries. For this reason, we studied the effect of the ethanol extracts of Radix Pulsatillae on the growth and acid production of S. mutans. Ethanol extracts of the Radix Pulsatillae showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by Radix Pulsatillae. the ethanol extract of Radix Pulsatillae showed remarkable inhibition of glucosyltransferase, which synthesizes water insoluble glucan form sucrose. Phytochemical analysis showed Radix Pulsatillae contained major components such as phenolic compounds, glycosides, steroids, terpenoid, and saponin. These results suggest that Radix Pulsatillae may have anti-cariogenic properties, which may be related with major components such as phenolic compounds, glycosides, steroids, terpenoid, and saponin.
Keywords
Radix Pulsatillae; Dental caries; Streptococcus mutans; Bacteria; Glucan; Adhesion;
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