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http://dx.doi.org/10.3746/jkfn.2010.39.7.1083

Inhibitory Mechanism of Daidzein on Helicobacter pylori Growth  

Bae, Kyung-Mi (Dept. of Food Science and Nutrition, Pusan National University)
Lee, Ju-Youn (Dept. of Food Science and Nutrition, Pusan National University)
Lee, Hee-Seob (Dept. of Food Science and Nutrition, Pusan National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.7, 2010 , pp. 1083-1086 More about this Journal
Abstract
This study was conducted to investigate the inhibitory effects of daidzein against H. pylori and its cholesterol $\alpha$-glucosyltransferase ($CHL{\alpha}GcT$). $CHL{\alpha}GcT$ is responsible for the production of $\alpha$-glucosyl cholesterol which constitutes more than 25% of cell wall lipids in H. pylori, and it has been suggested that it is essential for H. pylori viability. $CHL{\alpha}GcT$ was inhibited by daidzein, in a dose-dependant manner, of which $IC_{50}$ value was $128.5\;{\mu}M$. Daidzein also showed the inhibitory effect toward H. pylori growth by paper disc diffusion assay. Therefore, it is thought that the inhibition of daidzein on $CHL{\alpha}GcT$ was related to its anti-Helicobacter activity.
Keywords
Helicobacter pylori; cholesterol $\alpha$-glucosyltransferase ($CHL{\alpha}GcT$); inhibition; soy isoflavones; daidzein;
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1 Caragay AB. 1992. Cancer-preventive foods and ingredients. Food Technol 46: 566S-569S.
2 Clarkson TB. 2002. Soy, soy phytoestrogens and cardiovascular disease. J Nutr 132: 566S-569S.
3 Sarkar FH, Li YW. 2003. Soy isoflavones and cancer prevention. Cancer Invest 21: 744-757.   DOI
4 Eldridge AC. 1982. Determination of isoflavones in soybean flours, protein concentrates, and isolates. J Agric Food Chem 30: 353-355.   DOI
5 Murphy PA, Song T, Buseman G, Barua K, Beecher GR, Trainer D, Holden J. 1999. Isoflavones in retail and institutional soy foods. J Agric Food Chem 47: 2697-2704.   DOI
6 De Marino S, Borbone N, Gala F, Zollo F, Fico G, Pagiotti R, Iorizzi M. 2006. New constituents of sweet Capsicum annuum L. fruits and evaluation of their biological activity. J Agric Food Chem 54: 7508-7516.   DOI
7 Ustün O, Ozçelik B, Akyön Y, Abbasoglu U, Yesilada E. 2006. Flavonoids with anti-Helicobacter pylori activity from Cistus laurifolius leaves. J Ethnopharmacol 108: 457-461.   DOI
8 Lee H, Kobayashi M, Wang P, Nakayama J, Seeberger PH, Fukuda M. 2006. Expression cloning of cholesterol $\alpha$-glucosyltransferase, a unique enzyme that can be inhibited by natural antibiotic gastric mucin O-glycans, from Helicobacter pylori. Biochem Biophys Res Commun 349: 1235-1241.   DOI
9 Lee H, Wang P, Hoshino H, Ito Y, Kobayashi M, Nakayama J, Seeberger PH, Fukuda M. 2008. $\alpha$1,4GlcNAc-capped mucintype O-glycan inhibits cholesterol $\alpha$-glucosyltransferase from Helicobacter pylori and suppresses H. pylori growth. Glycobiology 18: 549-558.   DOI
10 Fukuda M, Kawakubo M, Ito Y, Kobayashi M, Lee H, Nakayama J. 2006. Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori. Methods Enzymol 415: 164-179.   DOI   ScienceOn
11 Vermeirssen V, Camp JV, Verstraete W. 2002. Optimization and validation of an angiotensin converting enzyme inhibition assay for the screening of bioactive peptides. J Biochem Biophys Methods 51: 75-87.   DOI
12 Jung KO, Kil JH, Kim KH, Park KY. 2003. Effect of kimchi and its ingredients on the growth of Helicobacter pylori. Nutraceuticals & Food 8: 149-153.   DOI
13 Lee JJ, Kim SH, Chang BS, Lee JB, Huh CS, Kim TJ, Baek YJ. 1999. The antimicrobial activity of medicinal plants extracts against Helicobacter pylori . Korean J Food Sci Technol 31: 764-770.   과학기술학회마을
14 Kim N. 2006. The effect of antibiotic resistance on the eradication of Helicobacter pylori. Korean J Gastroenterol 47: 82-86.
15 Marshall BJ, Warren JR. 1984. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1: 1311-1315.
16 Hidaka E, Ota H, Hidaka H, Hayama M, Matsuzawa T, Akamatsu T, Nakayama J, Katsuyama T. 2001. Helicobacter pylori and two ultrastructurally distinct layers of gastric mucous cell mucins in the surface mucous gel layer. Gut 49: 474-480.   DOI
17 Peek Jr RM, Blaser MJ. 2002. Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer 2: 28-37.   DOI
18 Hong YH. 2003. Functional foods. Chonnam National University Press, Kwangju, Korea. p 261.
19 Puupponen-Pimia R, Nohynek L, Hartmann-Schmidlin S, Kahkonen M, Heinonen M, Maatta-Riihinen K, Oksman-Caldentey KM. 2005. Berry phenolics selectively inhibit the growth of intestinal pathogens. J Appl Microbiol 98: 991-1000.   DOI
20 Correia RTP, McCue P, Vattem DA, Magalhaes MMA, Macedo GR, Shetty K. 2004. Amylase and Helicobacter pylori inhibition by phenolic extracts of pineapple wastes bioprocessed by Rhizopus oligosporus. J Food Biochem 28: 419-434.   DOI   ScienceOn
21 Naim M, Gestetner B, Bondi A, Birk Y. 1976. Antioxidative and antihemolytic activity of soybean isoflavone. J Agric Food Chem 22: 806-811.   DOI
22 Haque M, Hirai Y, Yokota K, Mori N, Jahan I, Ito H, Hotta H, Yano I, Kanemasa Y, Oguma K. 1996. Lipid profile of Helicobacter spp.: presence of cholesteryl glucoside as a characteristic feature. J Bacteriol 178: 2065-2070.   DOI
23 Hong HH, Landauer MR, Foriska MA, Ledney GD. 2006. Antibacterial activity of the soy isoflavone genistein. J Basic Microbiol 46: 329-335.   DOI