• 생명과학회지
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• 제22권6호
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• pp.730-735
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• 2012

본 연구에서는 신이화에 함유된 물질을 열수와 에탄올을 이용하여 추출한 후 화장품약리활성 및 항염증효과를 확인하고, 화장품 및 바이오산업에 적용하기 위한 천연소재로써의 이용 가능성을 살펴보았다. DPPH 라디칼 소거능 실험결과 농도의존적으로 소거능 효과를 보이며, 열수추출물의 경우 5,000 ug/ml 농도에서 대조군인 BHA보다 우수한 효능을 보였다. SOD 유사활성에서는 농도가 증가할수록 유사활성이 증가하는 것을 확인할 수 있었고, 5,000 ug/ml에서 열수추출물의 경우 85% 유사활성을 나타내었고, 에탄올 추출물의 경우 43%의 유사활성을 나타내었다. Xanthine oxidase 저해능 실험에서도 5,000 ug/ml 농도에서 대조군 BHA 보다 높은 저해능을 효여 우수한 항산화효과를 보였으며, 합성항산화제를 대체할 수 있는 천연항산화제로 사용이 가능할 것으로 사료된다. 항염증효과를 확인하기 위하여 NO 저해 활성 및 iNOS 발현량을 측정하였는데, 열수와 에탄올 추출물 모두 농도가 증가할 수로 NO 저해활성과 iNOS 발현량이 감소하여 우수한 항염증효과를 보였다. 이상의 결과에서 신이화 열수추출물과 에탄올추출물은 우수한 화장품약리활성 및 항염증효과가 있음을 확인할 수 있었고, 안티에이징 및 트러블 피부 개선 화장품 소재로서의 가능성을 확인할 수 있었다.

• Journal of the Korean Wood Science and Technology
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• 제38권6호
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• pp.579-586
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• 2010

목련과 식물의 활용도 제고를 위해 항산화제, 식품첨가제 개발에 필요한 항균 및 항산화 활성을 검정하였다. 우선 목련과 총 11종 식물의 수피, 잎, 목부, 꽃 및 열매의 에탄올 조추출물을 1,000 ${\mu}g/m{\ell}$로 조제하여 항진균 활성을 조사하였다. 그 결과 일본목련 수피가 백색부후균인 Trametes versicolor를 제외한 모든 공시균주에 대하여 50% 이상의 균사생장억제율을 나타내어 항균활성이 가장 높은 것으로 나타났다. 그 다음이 일본목련 목부로 탄저병균인 Glomerella cingulata와 목재부후균인 T. versicolor, Tyromyces palustris를 제외한 공시균주에 대하여 50% 이상의 균사생장억제율을 나타내었다. 수종별로는 일본목련이 가장 항균활성이 우수하여 수피, 잎, 목부 모두 항균활성이 있는 것으로 나타났으며 그 다음이 목련, 함박꽃나무 순이었다. 부위별로는 수피가 가장 활성이 우수하였으며 백목련, 자목련의 경우에는 다른 부위에 비해 꽃의 항균활성이 우수하였다. 또한, 프리라 디칼소거능을 측정하여 항산화활성을 검정한 결과에서는 100 ${\mu}g/m{\ell}$의 농도에서 태산목 수피와 잎이 70% 이상의 소거능을, 그리고 초령목 수피와 잎, 흑오미자 줄기 및 남오미자 줄기가 90% 이상의 소거능을 나타내었다. 특히, 흑오미자 줄기 및 남오미자 줄기는 10 ${\mu}g/m{\ell}$의 농도에서 각각 41.2%와 44.4%의 소거능을 나타내어 합성 항산화제인 butylated hydroxyanisole 및 천연항산화제인 ${\alpha}$-tocopherol과 동등한 항산화활성이 있는 것으로 밝혀졌다. 금후 흑오미자 및 남오미자 줄기와 같이 항산화활성 우수 시료는 활성물질 분리 및 구조 동정, 안전성 검정 등을 통하여 식품보존제 및 화장품 소재로 개발이 가능할 것으로 사료되었다.

• 한국식품영양과학회지
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• 제40권4호
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• pp.509-516
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• 2011

개똥쑥(Artemisia annua L.)의 생리활성에 관한 연구로써 개똥쑥 잎과 줄기의 항산화 및 암세포 증식억제 활성을 측정하였으며, 이들의 활성과 밀접한 관련이 있을 것으로 추정되는 페놀 화합물을 HPLC를 이용하여 분석 동정하였다. 개똥쑥 잎과 줄기 추출물의 항산화 활성은 농도에 의존적이었으며, 대부분 줄기보다 잎에서 더 높은 경향이었다. 인체 자궁경부 상피암 세포인 HeLa 및 위암세포인 AGS에 대한 증식억제 활성은 줄기보다 잎 추출물에서 더 높았는데 특히, 잎 에탄올 추출물의 HeLa 및 AGS세포에 대한 증식억제 활성은 $500\;{\mu}g$/mL 농도에서 각각 61.07% 및 57.24%로 추출물 중 가장 높은 활성을 보였다. HPLC를 이용하여 페놀화합물을 분석한 결과, phenolic acid 및 catechin류는 줄기에 비해 잎 추출물에서 월등히 높게 정량되었다. Flavonol류는 잎에서만 동정되었으며, rutin 및 kaempferol은 잎 에탄올 추출물에서만 동정되었다. 개똥쑥 추출물의 항산화 및 암세포 증식억제 활성은 페놀 화합물에 대한 의존도가 높으나, 미지의 활성 물질이 관여하는 것으로 추정된다.

• Applied Biological Chemistry
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• 제38권4호
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• pp.289-292
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• 1995

무화과잎으로부터 천연 항미생물 물질의 개발을 검토할 목적으로 무화과잎을 메탄올로 추출하고 ethyl acetate와 buffer용액을 이용하여 용매분획하였고 각 분획에 대한 항미생물 활성을 측정하였으며 활성이 강한 분획에 대한 최소저해농도(MIC)와 최소치사농도(MLC)를 측정하였다. 무화과 잎중의 항미생물 활성은 산성 분획과 페놀성 분획에서 강하게 나타났으며 미생물의 종류에 관계없이 활성범위가 넓었다. 페놀성 분획이 산성 분획에 비하여 MIC와 MLC값이 상대적으로 낮았고 MIC값의 경우 두 분획이 공통적으로 세균보다는 진균류에 대하여 낮았으며 특히 저온세균인 Pseudomonas aeruginosa에 대한 MIC와 MLC값이 대단히 낮음을 알 수 있었다.

• Preventive Nutrition and Food Science
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• 제20권1호
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• pp.8-14
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• 2015

Many studies on broccoli have analyzed the functional components and their functionality in terms of antioxidant and anticancer activities; however, these studies have focused on the florets of different varieties. Investigation of the functionality of broccoli by-products such as leaves, stems, and leaf stems from different cultivars and harvest dates might be valuable for utilizing waste materials as useful food components. Total phenolics and sulforaphane contents, and antioxidant and anticancer activities were measured in the leaves, leaf stems, and stems of early-maturing (Kyoyoshi), middle-maturing (Myeongil 96), and late-maturing broccoli (SK3-085) at different harvest dates. Total phenolics in the leaves of Kyoyoshi were about 1.8-fold to 12.1-fold higher than those in all of the other cultivars and parts. The sulforaphane content of Kyoyoshi was 2.8-fold higher in the stems than in the florets. Antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorbance capacity were highest in Kyoyoshi, followed by Myeongil 96 and SK3-085, most notably in the leaves harvested at the immature stage. Inhibition activity of cell growth against the NCI-H1299 cell lines was highest in the leaves of all cultivars in decreasing order of florets, leaf stems, and stems. The leaves harvested in October (nonflowering stage) had the highest inhibition activity, while those harvested in January (mature broccoli) showed the lowest. The results of this study demonstrate that broccoli leaves and stems contain high levels of total phenolics, and high antioxidant and anticancer activities and can provide opportunities for early-maturing broccoli as functional fresh raw vegetables.

• 한국자원식물학회지
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• 제26권3호
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• pp.403-409
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• 2013

This study has assessed the anti-oxidative activities and cytotoxic effects of Lithospermum erythrorhizon ethanol extract and measured the effects of tyrosinase inhibition activities with a goal of estimating the usage of the medicinal plant as an ingredient of cosmetics. First, to perform a basic test on the extract, pH and UV-spectrum were measured. According to the measurement, the extract had control functions at pH 5.5, and maximum absorbance occurred at 530nm. In particular, DPPH (1-1-diphenyl-2-picryl-hydrazyl)-inhibiting activity (IC50) and polyphenol content were 149.81 ${\mu}g/mL$ and $51.28{\pm}2.52$ mg/mL respectively. In addition, as extract concentration increased, tyrosinase inhibition activities improved as well. In raw 264.7 cell-based MTT assay, cell survival rates were 98% at 1000 ppm and 153% at 100 ppm. Therefore, it's been confirmed that there is almost no cytotoxin. According to the test results above, it appears that the Lithospermum erythrorhizon ethanol extract would be effective in anti-oxidation and application as a cosmetic ingredient.

• Journal of Nutrition and Health
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• 제29권6호
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• pp.608-614
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• 1996

This study was performed to investigate the influcence of mugwort on the duodenal ulcer induced by cysteamine administration in rats. Five groups of rats were fed each experimental diet containing 0%, 5%, 15%, 30% of mugwork powder for 10 weeks. Duodenal ulcer was induced by cysteamine injection (400mg/100g body weight) after 10 weeks of feeding experimental diets (C-0, C-5, C-15, C-30). Control animal that fed 0% mugwork powder added diet were injected saline (S-0) to compare with cysteamine injected groups. When the duodenal ulcer induced by cysteamine-HCI administration, all animals in the C-0 group formed erosion and perforating ulcer was found in 44% of animals. Higher the added mugwork ratio, more inhibited of the duodenal ulcer induced by cysteamine administration (C-5, C-15). But when the ratio of added mugwort is 30%, the inhibition effect disappeared (C-30). The alkaline phospatase activities were lower at the duodenal mucosa and small intestinal mucosa in the cysteamine treated groups(p<0.05). The acid phophatase activities were higher at the stomach, small intestine and large intestine of the cysteamine treated groups. But in mugwort added diet group, the changes of enzyme activites were lessended. The maltast activities were lower at the duodental mucosa and small intestinal mucosa of cysteamine treated groups. But in mugwort added diet group, maltase activites were recovered.

• 한국응용과학기술학회지
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• 제24권2호
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• pp.109-116
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• 2007

2-Chlorobenzoyl hydrazine refluxed with benzoyl isothiocyanate and phenyl isothiocyanate in ethanol for 3 hours to give benzamide derivative (1) and anilinederivative (2) on yield of 71%and 95%, respectively. Benzamide derivative (1) reacted with ethanolic sodium hydroxide on reflux to afford cyclization product (3), followed by general substitution reaction of two steps to give acetamide (5), and derivatived acetamides 7a-7k, while aniline derivative (2) reacted with ethanolic sodium hydroxide on reflux to afford another cyclization product (4). Thiol (4) reacted with N-phenyl chloroacetamide in the presence of potassim carbonate to give acetamide derivative (6). Compounds 1-7kwere evaluated for their growth inhibition against five cancer cell lines, including human lung carcinoma (A-549), human prostate cancer (DU145), human colon adenocarcinoma (HT-29), human malignant melanoma (SK-MEL-2) and human ovary malignant ascites (SK-OV-3) with sulforhodamine B (SRB) assay. All compounds (1-7k) showed low inhibition activities under 50% on 100M concentration.

• KSBB Journal
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• 제28권3호
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• pp.177-184
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• 2013

This research was to investigate the inhibitory effects of tyrosinase, elastase and collagenase using rapeseed meal extract for the functional cosmetics. Gamma-tocopherol and alpha-tocopherol contents were 304.9 and 212.2 mg/kg, respectively. In the case of delta-tocopherol and plastochromanol-8, they were 12.1 and 35.7 mg/kg, respectively. The total phenol content of methanol extract was the highest (49.6 mg/g) which was about 4.96 fold higher than that of water extract. The maximum nitrite scavenging activities of methanol and acetone extract at pH 1.2 were 85.2 and 80.1%, respectively, at 8.0 mg/mL. When the extract concentration of rapeseed meal increased upto 2.0 mg/mL, cell viabilities did not appear to have any significant cytotoxic effect, irrespective of extracts. Tyrosinase and elastase inhibition activities increased from 25.2 to 42.5% and 25.3 to 48.0%, respectively, as methanol extract concentration increased from 0.5 to 1.0 mg/mL. The collagenase inhibition activities of methanol and acetone extracts at 1.0 mg/mL were 67.2 and 68.0%, respectively. These results showed that the methanol and acetone extract of rapeseed meal can be used as a new source of functional cosmetic agent.

• 생약학회지
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• 제51권3호
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• pp.199-206
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• 2020

The aim of this study was to investigate the potential of Chaenomeles sinensis as a content of protocatechuic acid and physiological activities. The analyzed the content of protocatechuic acid of Chaenomeles sinensis water extract (CSW) according to different extract temperature and time for extraction. It was analyzed as 0.14±0.00 mg/g in the extract at 100℃ for 3 h. Protocatechuic acid content increased as extraction time and temperature increased. The activity of the CSW at 100℃ for 3 h was measured. As a result of measuring DPPH, ABTS radical scavenging activity and SOD activity, antioxidant activity was found and α-glucosidase inhibition activities. Cell viability was shown for RAW 264.7 cells, and NO (nitric oxide) production was also inhibited. Finally, content of protocatechuic acid increased with increasing extraction time and extraction temperature, and exhibited antioxidant activity, inhibition of NO production, and anti-diabetic activity. It is believed that the Chaenomeles sinensis is likely to be developed as a functional material.