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Phenolic Compounds from the Fruit Body of Phellinus linteus Increase Alkaline Phosphatase (ALP) Activity of Human Osteoblast-like Cells

  • Lyu, Ha-Na;Lee, Dae-Young;Kim, Dong-Hyun;Yoo, Jong-Su;Lee, Min-Kyung;Kim, In-Ho;Baek, Nam-In
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1214-1220
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    • 2008
  • Secondary metabolites from the fruit body of Phellinus linteus were evaluated for their proliferative effect on human osteoblast-like cells. 3-[4,5-Dimethylthiazole-2-y1]-2,5-diphenyl-tetraxolium bromide (MTT) assay and alkaline phosphatase (ALP) activity assay were used to assess the effect those isolates on the human osteoblast-like cell line (Saos-2). Activity-guided fractionation led to the isolation of ALP-activating phenolic compounds through the extraction of P. linteus, solvent partitioning, and repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations. From the result of spectroscopic data including nuclear magnetic resonance (NMR), mass spectrometry (MS), and infrared spectroscopy (IR), the chemical structures of the compounds were determined as 4-(4-hydroxyphenyl)-3-buten-2-one(1), 2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), 3,4-dihydroxybenzaldehyde (4), and protocatechuic acid methyl ester (5), respectively. This study reports the first isolation of compounds 1-3 and 5 from P. linteus. In addition, all phenolic compounds stimulated proliferation of the osteoblast-like cells and increased their ALP activity in a dose-dependent manner ($10^{-8}$ to $10^{-1}\;mg/mL$). The present data demonstrate that phenolic compounds in P. linteus stimulated mineralization in bone formation caused by osteoporosis. The bone-formation effect of P. linteus seems to be mediated, at least partly, by the stimulating effect of the phenolic compounds on the growth of osteoblasts.

A Study on the Development of Wearable Smart Fashion Product - Focused on the Construction of Optimized Functionalities for Particular Needs - (웨어러블 기능성 스마트 패션제품 개발 연구 - 특정사용자를 위한 특수한 기능성 구현을 중심으로 -)

  • Lee, Hyunseung;Lee, Jaejung
    • Fashion & Textile Research Journal
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    • v.21 no.2
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    • pp.133-140
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    • 2019
  • This study developed smart fashion prototypes that provide utilitarian functionality by combining Fashion and Electronics regarding the IT focused convergence tendency in modern industries. A convergence R&D workshop was performed by Fashion design majors and Engineering majors for the study. As a result, 5 functional smart fashion prototypes were developed and the outline of each prototype are as follows. The $1^{st}$ prototype, 'Hidden Camera Detecting Coat' focused on gender-related crimes. The coat uses infrared lighting and LED technologies to provide a function to detect hidden cameras in suspicious public spaces such as toilets. The $2^{nd}$ prototype, 'Heating-massage Suit' targeted patients with musculoskeletal system difficulties. The suit uses heating and vibration technologies to provide a heating massage treatment for patients with ongoing difficulties in their daily lives. The $3^{rd}$ prototype is an air-bag jacket to prevent sexual molestation on public transportation. The jacket extends its volume through pressure sensing, air compressing, motors and 3D-printing technology to secure the wearer's personal preventive space between the user's body and others. The $4^{th}$ prototype is a town wear for people suffering from synesthesia. People with synesthesia inadvertently see colors when exposed to certain sounds. This town wear uses sound sensing, air compressing, motors and 3D-printing technology to provide sound prevention and a comfortable sound playing function. The $5^{th}$ prototype is a set of a vest and a gloves for visually impaired people. The vest and gloves uses DMS, voice playing, vibration technology to provide distance measuring and warning functions.

Low Cost Alcoholic Breath Sensor Based on SnO2 Modified with CNTs and Graphene

  • Morsy, M.;Yahia, I. S.;Zahran, H.Y.;Ibrahim, M.
    • Journal of the Korean Physical Society
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    • v.73 no.10
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    • pp.1437-1443
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    • 2018
  • In this work, $SnO_2$ modified with reduced graphene oxide (rGO) and carbon nanotubes (CNTs) separately and combined sensitized by using the co-precipitation method and their sensing behavior toward ethanol vapor at room temperature were investigated. An interdigitated electrode (IDE) gold substrate is very expensive compared to a fluorine doped tin oxide (FTO) substrate; hence, we used the latter to reduce the fabrication cost. The structure and the morphology of the studied materials were characterized by using differential thermal analyses (DTA) and thermogravimetric analysis (TGA), transmission electron microscope (TEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, Brunauer-Emmett-Teller surface area and Barrett-Joyner-Halenda (BJH) pore size measurements. The studied composites were subjected to ethanol in its gas phase at concentrations from 10 to 200 ppm. The present composites showed high-performance sensitivity for many reasons: the incorporation of $SnO_2$ and CNTs which prevents the agglomeration of rGO sheets, the formation of a 3D mesopourus structure and an increase in the surface area. The decoration with rGO and CNTs led to more active sites, such as vacancies, which increased the adsorption of ethanol gas. In addition, the mesopore structure and the nano size of the $SnO_2$ particles allowed an efficient diffusion of gases to the active sites. Based on these results, the present composites should be considered as efficient and low-cost sensors for alcohol.

Portable titrator equipped spectroscopic detectors; Spectrator (분광학적 검출기가 내장된 휴대용 적정기: 스펙트레이터)

  • Shin, Jiwon;Chae, Gyoyoon;Kim, Yeajin;Kim, Sangho;Chae, Yoonsu;Chae, Won-Seok
    • Analytical Science and Technology
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    • v.34 no.3
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    • pp.128-133
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    • 2021
  • During titration, several chemical reactions result in changes not only in the potential of chemicals, but also in the colors of the indicator. In a potentiometric titration, a titration curve is obtained by measuring the abrupt change in the potential at the endpoint. Generally, acid-base titration is performed by observing the color change caused by an indicator to determine the endpoint. The method of determining the endpoint by measuring the potential difference has been well established and commercialized; however, the devices that can obtain the endpoint by observing the color change are limited. Consequently, in this study, a simple and precise spectral endpoint detector was manufactured using a drop-counter comprising an infrared emitter and a phototransistor, a white light LED as the light source and photodetector, and an analog-to-digital converter (Arduino). Spectrator, a new named, showed excellent results in terms of the reproducibility of acid-base titration using thymol blue as an indicator. Herein, we present the results of the Spectrator-manufacturing process as well as the experimental results.

Development of a Backpack-Based Wearable Proximity Detection System

  • Shin, Hyungsub;Chang, Seokhee;Yu, Namgyenong;Jeong, Chaeeun;Xi, Wen;Bae, Jihyun
    • Fashion & Textile Research Journal
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    • v.24 no.5
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    • pp.647-654
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    • 2022
  • Wearable devices come in a variety of shapes and sizes in numerous fields in numerous fields and are available in various forms. They can be integrated into clothing, gloves, hats, glasses, and bags and used in healthcare, the medical field, and machine interfaces. These devices keep track individuals' biological and behavioral data to help with health communication and are often used for injury prevention. Those with hearing loss or impaired vision find it more difficult to recognize an approaching person or object; these sensing devices are particularly useful for such individuals, as they assist them with injury prevention by alerting them to the presence of people or objects in their immediate vicinity. Despite these obvious preventive benefits to developing Internet of Things based devices for the disabled, the development of these devices has been sluggish thus far. In particular, when compared with people without disabilities, people with hearing impairment have a much higher probability of averting danger when they are able to notice it in advance. However, research and development remain severely underfunded. In this study, we incorporated a wearable detection system, which uses an infrared proximity sensor, into a backpack. This system helps its users recognize when someone is approaching from behind through visual and tactile notification, even if they have difficulty hearing or seeing the objects in their surroundings. Furthermore, this backpack could help prevent accidents for all users, particularly those with visual or hearing impairments.

Characteristics of Magnetic Sengon Wood Impregnated with Nano Fe3O4 and Furfuryl Alcohol

  • Gilang Dwi LAKSONO;Istie Sekartining RAHAYU;Lina KARLINASARI;Wayan DARMAWAN;Esti PRIHATINI
    • Journal of the Korean Wood Science and Technology
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    • v.51 no.1
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    • pp.1-13
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    • 2023
  • Sengon (Falcataria moluccana Miq.) tree offers a wood of low quality and durability owing to its low density and thin cell walls. This study aimed to improve the properties of sengon wood by making the wood magnetic, producing new functions, and characterizing magnetic sengon wood. Each wood sample was treated using one of the following impregnation solutions: Untreated, 7.5% nano magnetite-furfuryl alcohol (Fe3O4-FA), 10% nano Fe3O4-FA, and 12.5% nano Fe3O4-FA. The impregnation process began with vacuum treatment at 0.5 bar for 2 h, followed by applying a pressure of 1 bar for 2 h. The samples were then tested for dimensional stability and density and characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM-EDX), Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD) analysis, and vibrating sample magnetometry (VSM) analysis. The results showed that the Fe3O4-FA impregnation treatment considerable affected the dimensional stability, measured in terms of weight percent gain, anti-swelling efficiency, water uptake, and bulking effect, as well as the density of sengon wood. Changes in wood morphology were detected by the presence of Fe deposits in the cell walls and cell cavities of the wood using SEM-EDX analysis. XRD and FTIR analyses showed the appearance of magnetite peaks in the diffractogram and Fe-O functional groups. Based on the VSM analysis, treated sengon wood is classified as a superparamagnetic material with soft magnetic properties. Overall, 10% Fe3O4-FA treatment led to the highest increase in dimensional stability and density of sengon wood.

Design and Implementation of Smart Self-Learning Aid: Micro Dot Pattern Recognition based Information Embedding Solution (스마트 학습지: 미세 격자 패턴 인식 기반의 지능형 학습 도우미 시스템의 설계와 구현)

  • Shim, Jae-Youen;Kim, Seong-Whan
    • Proceedings of the Korea Information Processing Society Conference
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    • 2011.04a
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    • pp.346-349
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    • 2011
  • In this paper, we design a perceptually invisible dot pattern layout and its recognition scheme, and we apply the recognition scheme into a smart self learning aid for interactive learning aid. To increase maximum information capacity and also increase robustness to the noises, we design a ECC (error correcting code) based dot pattern with directional vector indicator. To make a smart self-learning aid, we embed the micro dot pattern (20 information bit + 15 ECC bits + 9 layout information bit) using K ink (CMYK) and extract the dot pattern using IR (infrared) LED and IR filter based camera, which is embedded in the smart pen. The reason we use K ink is that K ink is a carbon based ink in nature, and carbon is easily recognized with IR even without light. After acquiring IR camera images for the dot patterns, we perform layout adjustment using the 9 layout information bit, and extract 20 information bits from 35 data bits which is composed of 20 information bits and 15 ECC bits. To embed and extract information bits, we use topology based dot pattern recognition scheme which is robust to geometric distortion which is very usual in camera based recognition scheme. Topology based pattern recognition traces next information bit symbols using topological distance measurement from the pivot information bit. We implemented and experimented with sample patterns, and it shows that we can achieve almost 99% recognition for our embedding patterns.

Colloidal Optics and Photonics: Photonic Crystals, Plasmonics, and Metamaterials

  • Jaewon Lee;Seungwoo Lee
    • Current Optics and Photonics
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    • v.7 no.6
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    • pp.608-637
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    • 2023
  • The initial motivation in colloid science and engineering was driven by the fact that colloids can serve as excellent models to study atomic and molecular behavior at the mesoscale or microscale. The thermal behaviors of actual atoms and molecules are similar to those of colloids at the mesoscale or microscale, with the primary distinction being the slower dynamics of the latter. While atoms and molecules are challenging to observe directly in situ, colloidal motions can be easily monitored in situ using simple and versatile optical microscopic imaging. This foundational approach in colloid research persisted until the 1980s, and began to be extensively implemented in optics and photonics research in the 1990s. This shift in research direction was brought by an interplay of several factors. In 1987, Yablonovitch and John modernized the concept of photonic crystals (initially conceptualized by Lord Rayleigh in 1887). Around this time, mesoscale dielectric colloids, which were predominantly in a suspended state, began to be self-assembled into three-dimensional (3D) crystals. For photonic crystals operating at optical frequencies (visible to near-infrared), mesoscale crystal units are needed. At that time, no manufacturing process could achieve this, except through colloidal self-assembly. This convergence of the thirst for advances in optics and photonics and the interest in the expanding field of colloids led to a significant shift in the research paradigm of colloids. Initially limited to polymers and ceramics, colloidal elements subsequently expanded to include semiconductors, metals, and DNA after the year 2000. As a result, the application of colloids extended beyond dielectric-based photonic crystals to encompass plasmonics, metamaterials, and metasurfaces, shaping the present field of colloidal optics and photonics. In this review we aim to introduce the research trajectory of colloidal optics and photonics over the past three decades; To elucidate the utility of colloids in photonic crystals, plasmonics, and metamaterials; And to present the challenges that must be overcome and potential research prospects for the future.

Improvement and Validation of Convective Rainfall Rate Retrieved from Visible and Infrared Image Bands of the COMS Satellite (COMS 위성의 가시 및 적외 영상 채널로부터 복원된 대류운의 강우강도 향상과 검증)

  • Moon, Yun Seob;Lee, Kangyeol
    • Journal of the Korean earth science society
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    • v.37 no.7
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    • pp.420-433
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    • 2016
  • The purpose of this study is to improve the calibration matrixes of 2-D and 3-D convective rainfall rates (CRR) using the brightness temperature of the infrared $10.8{\mu}m$ channel (IR), the difference of brightness temperatures between infrared $10.8{\mu}m$ and vapor $6.7{\mu}m$ channels (IR-WV), and the normalized reflectance of the visible channel (VIS) from the COMS satellite and rainfall rate from the weather radar for the period of 75 rainy days from April 22, 2011 to October 22, 2011 in Korea. Especially, the rainfall rate data of the weather radar are used to validate the new 2-D and 3-DCRR calibration matrixes suitable for the Korean peninsula for the period of 24 rainy days in 2011. The 2D and 3D calibration matrixes provide the basic and maximum CRR values ($mm\;h^{-1}$) by multiplying the rain probability matrix, which is calculated by using the number of rainy and no-rainy pixels with associated 2-D (IR, IR-WV) and 3-D (IR, IR-WV, VIS) matrixes, by the mean and maximum rainfall rate matrixes, respectively, which is calculated by dividing the accumulated rainfall rate by the number of rainy pixels and by the product of the maximum rain rate for the calibration period by the number of rain occurrences. Finally, new 2-D and 3-D CRR calibration matrixes are obtained experimentally from the regression analysis of both basic and maximum rainfall rate matrixes. As a result, an area of rainfall rate more than 10 mm/h is magnified in the new ones as well as CRR is shown in lower class ranges in matrixes between IR brightness temperature and IR-WV brightness temperature difference than the existing ones. Accuracy and categorical statistics are computed for the data of CRR events occurred during the given period. The mean error (ME), mean absolute error (MAE), and root mean squire error (RMSE) in new 2-D and 3-D CRR calibrations led to smaller than in the existing ones, where false alarm ratio had decreased, probability of detection had increased a bit, and critical success index scores had improved. To take into account the strong rainfall rate in the weather events such as thunderstorms and typhoon, a moisture correction factor is corrected. This factor is defined as the product of the total precipitable waterby the relative humidity (PW RH), a mean value between surface and 500 hPa level, obtained from a numerical model or the COMS retrieval data. In this study, when the IR cloud top brightness temperature is lower than 210 K and the relative humidity is greater than 40%, the moisture correction factor is empirically scaled from 1.0 to 2.0 basing on PW RH values. Consequently, in applying to this factor in new 2D and 2D CRR calibrations, the ME, MAE, and RMSE are smaller than the new ones.

Development on New Laser Tabbing Process for Modulation of Thin Solar Cell (박형 태양 전지 모듈화를 위한 레이져 태빙 자동화 공정(장비) 개발)

  • No, Donghun;Choi, Chul-June;Cho, Hyun Young;Yu, Jae Min;Kim, JungKeun
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.58.1-58.1
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    • 2010
  • In solar cell module manufacturing, single solar cells has to be joined electrically to strings. Copper stripes coated with tin-silver-copper alloy are joined on screen printed silver of solar cells which is called busbar. The bus bar collects the electrons generated in solar cell and it is connected to the next cell in the conventional module manufacturing by a metal stringer using conventional hot air or infrared lamp soldering systems. For thin solar cells, both soldering methods have disadvantages, which heats up the whole cell to high temperatures. Because of the different thermal expansion coefficient, mechanical stresses are induced in the solar cell. Recently, the trend of solar cell is toward thinner thickness below 180um and thus the risk of breakage of solar cells is increasing. This has led to the demand for new joining processes with high productivity and reduced error rates. In our project, we have developed a new method to solder solar cells with a laser heating source. The soldering process using diode laser with wavelength of 980nm was examined. The diode laser used has a maximum power of 60W and a scanner system is used to solder dimension of 6" solar cell and the beam travel speed is optimized. For clamping copper stripe to solar cell, zirconia(ZrO)coated iron pin-spring system is used to clamp both joining parts during a scanner system is traveled. The hot plate temperature that solar cell is positioned during lasersoldering process is optimized. Also, conventional solder joints after $180^{\circ}C$ peel tests are compared to the laser soldering methods. Microstructures in welded zone shows that the diffusion zone between solar cell and metal stripes is better formed than inIR soldering method. It is analyzed that the laser solder joints show no damages to the silicon wafer and no cracks beneath the contact. Peel strength between 4N and 5N are measured, with much shorter joining time than IR solder joints and it is shown that the use of laser soldering reduced the degree of bending of solar cell much less than IR soldering.

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