• Journal of Microbiology and Biotechnology
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• v.29 no.5
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• pp.667-686
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• 2019

Streptomyces are attractive microbial cell factories that have industrial capability to produce a wide array of bioactive secondary metabolites. However, the genetic potential of the Streptomyces species has not been fully utilized because most of their secondary metabolite biosynthetic gene clusters (SM-BGCs) are silent under laboratory culture conditions. In an effort to activate SM-BGCs encoded in Streptomyces genomes, synthetic biology has emerged as a robust strategy to understand, design, and engineer the biosynthetic capability of Streptomyces secondary metabolites. In this regard, diverse synthetic biology tools have been developed for Streptomyces species with technical advances in DNA synthesis, sequencing, and editing. Here, we review recent progress in the development of synthetic biology tools for the production of novel secondary metabolites in Streptomyces, including genomic elements and genome engineering tools for Streptomyces, the heterologous gene expression strategy of designed biosynthetic gene clusters in the Streptomyces chassis strain, and future directions to expand diversity of novel secondary metabolites.

• Membrane and Water Treatment
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• v.13 no.5
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• pp.245-257
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• 2022

Water quality characteristics and their spatial variations in the Nakdong River were statistically analyzed by multivariate techniques including correlation analysis, CA, and FA/PCA based on water quality parameters for 17 sites over 2017-2019, yielding PI values for primary factors. Site 10 indicated the highest parameter concentrations, and results of pearson's correlation analysis suggest that non-biodegradable organic matter had been distributed on the site. Five clusters were identified in order of descending pollution levels: I (Ib > Ia) > II (IIa > IIb) > III. Spatial variations started from sub-cluster Ib in which Daegu city and Geumho-river are joined. T-P, PO4-P, SS, COD, and TOC corresponded to VF 1 and 2, which were found to be principal components with strong influence on water quality. Sub-cluster Ib was strongly influenced by NO3-N and T-N compared to other clusters. According to the PIs, water quality pollution deteriorated due to non-biodegradable organic matter, nitrogen- and phosphorus-based nutrient salts in the middle and lower reaches, illustrating worsening water pollution due to inflows of anthropogenic sources on the Geumho-river, i.e., sewage and wastewater, discharged from Site 10, at which there is a concentration of urban, agricultural, and industrial areas.

• International Journal of Computer Science & Network Security
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• v.22 no.10
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• pp.107-112
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• 2022

Nowadays clusters are recognized as an important instrument for promoting industrial development, innovation, competitiveness and growth. An educational cluster is a set of interrelated vocational educational institutions of various levels that are united by industry with each other and are connected by partnership with industry enterprises. This article attempts to develop and describe cluster model of university students' multilingual training. The purpose of this study is to describe multilingual training of university students and their polycultural competencies formation and to define the process of multilingual training in form of a cluster. The authors consider clusters as an integral part of the educational campus within the concept framework of Shadrinsk State Pedagogical University. To determine the essence of the concept of a cluster model of university students' multilingual training, theoretical, empirical, observational, and diagnostic methods were implemented, such as a review of scientific literature, a compilation of best practices, observation, statistical methods, etc. The authors analyzed the programs of partner universities and organized international webinars and internships for bachelors and masters abroad and developed online courses "Foreign language for undergraduate students and masters". Experimental data obtained during the implementation of cluster training show the effectiveness of the formation of students' polycultural competencies.

• Journal of the Ergonomics Society of Korea
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• v.26 no.4
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• pp.103-111
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• 2007

The purpose of this study is to categorize and analyze the body shape of obese Korean men that are needed for industrial design. Using the anthropometric data that were surveyed through the 5^th Size Korea project, this study was conducted in four steps mostly through the multivariate statistical analysis. In the first step, Broca, BMI, WHR indices are used to define obesity and select obese men from Korean adults and teens. After 34 human anthropometric variables are supposed to be related to obesity were extracted through an expect survey. In the second step, a factor analysis was executed for those human anthropometric variables. Through this analysis, we obtained the human body factors that are related to the representation of obesity. Then the third step, we used a cluster analysis from the result of the factor analysis. And ANOVA analysis was also conducted to obtain the critical obese human anthropometric variables. In the final step, we found the characteristics of the body types of obese men according to clusters and ages. The body types of obese men classified in the study are expected to be applied to product design for clothing, furniture, automobile packaging, etc.

• Fisheries and Aquatic Sciences
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• v.22 no.8
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• pp.17.1-17.9
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• 2019

Bacillus is a diverse genus consisting of more than 200 species with extensive genetic diversity. Their beneficial effects in industrial shrimp farming have been well documented. However, little is known about the biodiversity of the Bacillus spp. in this aquaculture system. Taxonomic analysis by 16S rRNA sequencing does not always allow species-level identification of Bacillus spp. In this study, 26 Bacillus isolates from two industrial Litopenaeus vannamei shrimp ponds in Bac Lieu Province, Vietnam, were analyzed for their genetic diversity by multi-locus sequence typing (MLST). A total of 22 sequence types were identified and segregated into four distinct clusters, corresponding to B. subtilis, B. velezensis, B. siamensis, and B. licheniformis. Bacillus subtilis and B. velezensis accounted for more than 73% of the Bacillus isolates. Notably, the MLST scheme exhibited high discriminatory power and might be further simplified to be a convenient method to identify species of the genus Bacillus.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.6
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• pp.629-641
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• 2016

This study analyzed the use of the phenomenological method proposed by Colaizzi (1978) in order to understand the meaning of block system class experiences in nursing students. Furthermore, this study provides primal data to improve curriculum efficiency. The data were collected from 18 participants who were students at a nursing university in the city of G from 20th of June to 10th of July 2015. This study investigated nursing student's block system class experience. The study results show six theme clusters consisting of 'Discomfort', 'Unsystematic study', 'Holding back an urge', 'Ever intensifying relationship', 'New discovery', and 'Chance for development', 17 themes, and 42 sub themes, which are included in those clusters in 58 configured meanings. This demonstrates that nursing students could reestablish their resolve for personal betterment and realize the importance of managing time by finishing the procedure with their best efforts although they suffer from physical and psychological inconvenience due to massive amounts of learning and assignment. These results indicate the need for development of stress management and an emotional support program based on the experience of nursing students.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.4 no.2
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• pp.108-113
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• 2003

Research results have demonstrated that conventional client-server databases have scalability problem in the presence of many concurrent clients. The multi-tier architecture that exploits similarities in clients' object access behavior partitions clients into logical clusters according to their object request pattern. As a result, object requests that are served inside the clusters, server load and request response time can be optimized. Management of clustering by utilizing clients' access pattern-based is an important component for the system's goal. Off-line methods optimizes the quality of the global clustering, the necessary cost and clustering schedule needs to be considered and planned carefully in respect of stable system's performance. In this paper, we propose methods that detect changes in access behavior and optimize system configuration in real time. Finally this paper demonstrates the effectiveness of on-line change detection and results of experimental investigation concerning reconfiguration.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.7
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• pp.263-272
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• 2016

Modular product design has contributed to flexible product modification and development, production lead time reduction, and increasing product diversity. Modular product design aims to develop a product architecture that is composed of detachable modules. These modules are constructed by maximizing the similarity of components based on physical and functional interaction analysis among components. Accordingly, a systematic procedure for clustering the components, which is a main activity in modular product design, is proposed in this paper. The first phase in this procedure is to build a component-to-component correlation matrix by analyzing physical and functional interaction relations among the components. In the second phase, network flow modeling is applied to find clusters of components, maximizing their correlations. In the last phase, a network flow model formulated with linear programming is solved to find the clusters and to make them modular. Finally, the proposed procedure in this research and its application are illustrated with an example of modularization for a vacuum cleaner.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.309-322
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• 2017

The purpose of this qualitative study was to describe the experience of mothers who have had children with cancer. Participants were seven mothers who had children diagnosed with cancer between 3 to 36 months. Data were gathered through in-depth interviews and analyzed by Colaizzi's phenomenological methodology. Overall, 216 significant statements, 62 formulated statements, 16 themes and five theme clusters were identified. The five theme clusters were 'heartbreaking sadness', 'arduous journey of battling', 'protect my child', 'feeling varying between gratitude and disappointment', and 'suffering and wishing to be with family'. Mothers who have had children with pediatric cancer receiving chemotherapy have experienced strong emotional fluctuations as well as hope as they cope with their ill child. However, they try to become strong women and embrace their family to cope with their situation. The results of this study will support for health professionals to understand mothers who have had children with cancer and to consider the supportive nursing care in considering mothers' emotional fluctuation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.9
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• pp.473-479
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• 2020

This study examined the meaning of the experiences of university students majoring in occupational therapy, where their practice was discontinued due to COVID-19, and to provide basic data for future measures. This study was a phenomenological study. The subjects of this study were seven students. Interviews were analyzed according to Colaizzi data analysis. As a result of an interview, it was organized into 17 themes, six themes clusters, and two categories. The themes clusters were indifference, problem recognition, wandering, coping, aftermath, and anticipation for coping. The students experienced a confused and frustrating situation after recognizing the problem of COVID-19 and experienced anxiety and regret as they experienced the interruption of their fieldwork. The students expected all universities to implement a batch and quick response at the same time. In the future, a systematic communication and delivery system is needed to quickly prepare and implement countermeasures at the same time as institutions, associations, and universities.