• Journal of Korean Biological Nursing Science
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• 제12권1호
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• pp.16-23
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• 2010

Purpose: The purpose of this study was to examine the effects of aromatherapy on blood pressure, pulse, fatigue and sleep for patients with allergic rhinitis admitted at a hospital specialized in an oriental medicine. Methods: This research was a single-group pretest-posttest design. Data were collected at admission and discharge from May to December, 2009. The patients selected were received an aroma inhalation therapy via nebulizer with a 2% diluted oil mixture of eucalyptus and peppermint with 10 cc of saline solution in the ratio of 1:1 ones a day for 15 min in the period of 2 weeks. The data were analyzed for the descriptive statistics and paired t-test. Results: There was a statistically significant improvement on physical and psychological symptoms of fatigue and sleep with a increase in systolic blood pressure. Conclusion: Eucalyptus and peppermint blended oil aromatherapy as a sleep induction modality is an effective nursing and therapeutic intervention in conjunction with acupuncture and herbal medicine for symptoms of nasal congestion. However, further well designed research is required in evaluating the effectiveness of the types of clinically available essential oil.

• Journal of Korean Biological Nursing Science
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• 제12권2호
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• pp.81-88
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• 2010

Purpose: The purpose of this study was to compare effects of intravenous fluid warming and forced-air warming on perioperative body temperature, Blood Pressure, Pulse and thermal discomfort after gastrectomy under general anesthesia. Methods: Data collection was performed from October, 2009 to February, 2010. The intravenous fluid warming group (27) was warmed through an IV line by an Animec set to $37^{\circ}C$. The forced-air warming group (27) was warmed by Bair Hugger System. The warming continued from induction of general anesthesia to two hours after completion of surgery. The data was analyzed by t-test, $X^2$, repeated measures ANOVA using SPSS/WIN 17.0. Results: There was a significant difference of body temperature and thermal discomfort between the intravenous fluid warming group and the forced-air warming group. Conclusion: We need to explore the effects combination of the intravenous fluid warming and the forced-air warming, and other warming therapy and the efficiency of modalities with regards to cost benefit is also needed.

• 생약학회지
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• 제35권4호통권139호
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• pp.283-287
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• 2004

The effect of water extract from Cnidii Rhizoma (CRW) on proliferation of human breast cancer cells, nitric oxide production, nitric oxide synthase expression, and ornithine decarboxylase activity was tested. CRW inhibited the growth of both estrogen-dependent MCF-7 and estrogen-independent MDA-MB-23I human breast cancer cells. Lipopolysaccharide-induced nitric oxide (NO) production was significantly reduced by CRW at the concentration of 0.5, 1.0 and 5.0 mg/ml. Expression of inducible nitric oxide synthase (iNOS) was also suppressed with the treatment of CRW in Raw 264.7 cells. CRW inhibited induction of ornithine decarboxylase by 12-0-tetradecanoylphorbol-13-acetate, a key enzyme of polyamine biosynthesis, which is enhanced in tumour promotion. Therefore, CRW is worth further investigation with respect to breast cancer chemoprevention or therapy.

• 정신신체의학
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• 제14권1호
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• pp.18-24
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• 2006

불면증은 다양한 신체적, 정신적 원인에 의해 생기는데, 특히 과잉각성은 불면증에 중요한 영향을 미친다고 알려져 있다. 불면증 환자에서 약물치료에 한계가 있으며 심리적 요인이 불면증의 발생과 경과에 큰 영향을 미친다는 사실이 알려지면서, 여러 가지 비약물치료적 방법이 도입되었다. 바이오피드백을 포함한 비약물치료는 불면증 치료에 효과적이며 치료 효과도 오래 유지되는 것으로 보고되었다. 바이오피드백은 이완요법과 함께 사용 시 불면증 환자의 각성 수준을 낮춰 수면의 시작 및 유지에 도움이 되므로, 각성 수준이 높거나 약물 사용에 제한이 따르는 불면증 환자에게 매우 유용할 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2807-2811
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• 2015

Scorpion venom peptides recently have attracted attention as alternative chemotherapeutic agents that may overcome the limitations of current drugs, providing specific cytotoxicity for cancer cells with an ability to bypass multidrug-resistance mechanisms, additive effects in combination therapy and safety. In the present study, BmKn-2 scorpion venom peptide and its derivatives were chosen for assessment of anticancer activities. BmKn-2 was identified as the most effective against human oral squamous cells carcinoma cell line (HSC-4) by screening assays with an $IC_{50}$ value of $29{\mu}g/ml$. The BmKn-2 peptide killed HSC-4 cells through induction of apoptosis, as confirmed by phase contrast microscopy and RT-PCR techniques. Typical morphological features of apoptosis including cell shrinkage and rounding characteristics were observed in treated HSC-4 cells. The results were further confirmed by increased expression of pro-apoptotic genes such as caspase-3, -7, and -9 but decrease mRNA level of anti-apoptotic BCL-2 in BmKn-2 treated cells, as determined by RT-PCR assay. In summary, the BmKn-2 scorpion venom peptide demonstrates specific membrane binding, growth inhibition and apoptogenic activity against human oral cancer cells.

• BMB Reports
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• 제47권4호
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• pp.184-191
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• 2014

Respiratory syncytial virus (RSV) is the leading cause of respiratory infection in infants and young children. Severe clinical manifestation of RSV infection is a bronchiolitis, which is common in infants under six months of age. Recently, RSV has been recognized as an important cause of respiratory infection in older populations with cardiovascular morbidity or immunocompromised patients. However, neither a vaccine nor an effective antiviral therapy is currently available. Moreover, the interaction between the host immune system and the RSV pathogen during an infection is not well understood. The innate immune system recognizes RSV through multiple mechanisms. The first innate immune RSV detectors are the pattern recognition receptors (PRRs), including toll-like receptors (TLRs), retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), and nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs). The following is a review of studies associated with various PRRs that are responsible for RSV virion recognition and subsequent induction of the antiviral immune response during RSV infection.

• 대한한방부인과학회지
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• 제28권2호
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• pp.15-25
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• 2015

Objectives : In this study, we investigated the anti-proliferative and apoptosis inducing effect of water extract of Chelidonium majus (CM) on human breast cancer cell MDA-MB-231. Methods : The MTT assay was used to assess cell proliferation. The expression of apoptosis related gene was assessed by quantitative Real-time PCR. Cell apoptosis detected by flow cytometry using Annexin-V/PI staining. Results : Our data revealed that CM inhibited the cell growth in a dose dependent manner (0, 62.5, 125, 250, 500 μg/ml). CM increased mRNA expression of pro-apoptotic genes Bax, caspase-3, and caspase-9. Annexin-V/PI staining assays revealed that apoptosis-induced cell death increased in a dose-dependent manner in cells. Conclusions : CM induces cell death in MDA-MB-231 human breast cancer cell and shows potentials for use in cancer therapy as apoptosis-inducing agent.

• Genomics & Informatics
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• 제6권2호
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• pp.84-86
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• 2008

The Tetrahymena group I intron has been shown to employ a trans-splicing reaction and has been modified to specifically target and replace human telomerase reverse transcriptase (hTERT) RNA with a suicide gene transcript, resulting in the induction of selective cytotoxicity in cancer cells that express the target RNA, in animal models as well as in cell cultures. In this study, we evaluated the target RNA specificity of trans-splicing phenomena by the group I intron in mice that were intraperitoneally inoculated with hTERT-expressing human cancer cells to validate the anti-cancer therapeutic applicability of the group I intron. To this end, an adenoviral vector that encoded for the hTERT-targeting group I intron was constructed and systemically injected into the animal. 5'-end RACE-PCR and sequencing analyses of the trans-spliced cDNA clones revealed that all of the analyzed products in the tumor tissue of the virus-infected mice resulted from reactions that were generated only with the targeted hTERT RNA. This study implies the in vivo target specificity of the trans-splicing group I intron and hence suggests that RNA replacement via a trans-splicing reaction by the group I intron is a potent anti-cancer genetic approach.

• Asian Pacific Journal of Cancer Prevention
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• 제17권sup3호
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• pp.299-304
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• 2016

Cytolethal distending toxin (CDT) is a secreted tripartite genotoxin produced by many pathogenic gram-negative bacteria. It is composed of three subunits, CdtA, CdtB and CdtC, and CdtB-associated deoxyribonuclease (DNase) activity is essential for the CDT toxicity. In the present study, to design a novel potentially antitumor drug against lung cancer, the possible mechanisms of cdtB anticancer properties were explored in the A549 human lung adenocarcinoma cell line. A recombinant plasmid pcDNA3.1/cdtB was constructed expressing CdtB of human periodontal bacterium Aggregatibacter actinomycetemcomitans and investigated for toxic properties in A549 cells and possible mechanisms. It was observed that plasmid pcDNA3.1/cdtB caused loss of cell viability, morphologic changes and induction of apoptosis. Furthermore, measurement of caspase activity indicated involvement of an intrinsic pathway of cell apoptosis. Consequently, the recombinant plasmid pcDNA3.1/cdtB may have potential as a new class of therapeutic agent for gene therapy of lung cancer.

• 대한의생명과학회지
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• 제7권4호
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• pp.205-210
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• 2001

Whereas apoptosis is a critical mode of cell deletion in normal organism development, apoptotic cells are also observed in tumor therapy. We therefore investigated the expression of apoptotic cells induced as a function of time and dose in murine A-20 lymphoma treated with cyclophosphamide in vivo, by H&E and TUNEL method. The percent of apoptotic cells were scored from tumor section using TUNEL method. The expression of apoptotic positive cell was determined over a 10-day period following treatment of the mice with 200 mg/kg. Apoptosis increased further with time, reaching a peak value between 12~24 hr (scored 6.7$\pm$1.0%~6.1$\pm$0.7%), and then slowly declined to background levels by 10 days after treatment. The dependence of induction of apoptosis on the dose of cyctophosphamide was determined by treatment with 50, 100, or 200 mg/kg at 12 hr after treatment. Apoptosis was dose dependent in that as the dose was increased the percentage of apoptosis increased. However, the increase in apoptosis at the lower dose used (50 mg/kg) was higher on a per unit dose basis than that at the higher dose used (200 mg/kg). This result show that the alkylating agent cyclophosphamide strongly induces apoptosis in murine lymphoma.