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http://dx.doi.org/10.15204/jkobgy.2015.28.2.015

Effects of Chelidonium Majus Extract on Apoptosis Induction of MDA-MB-231 Human Breast Cancer Cells  

Jang, Sae-Byul (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.28, no.2, 2015 , pp. 15-25 More about this Journal
Abstract
Objectives : In this study, we investigated the anti-proliferative and apoptosis inducing effect of water extract of Chelidonium majus (CM) on human breast cancer cell MDA-MB-231. Methods : The MTT assay was used to assess cell proliferation. The expression of apoptosis related gene was assessed by quantitative Real-time PCR. Cell apoptosis detected by flow cytometry using Annexin-V/PI staining. Results : Our data revealed that CM inhibited the cell growth in a dose dependent manner (0, 62.5, 125, 250, 500 μg/ml). CM increased mRNA expression of pro-apoptotic genes Bax, caspase-3, and caspase-9. Annexin-V/PI staining assays revealed that apoptosis-induced cell death increased in a dose-dependent manner in cells. Conclusions : CM induces cell death in MDA-MB-231 human breast cancer cell and shows potentials for use in cancer therapy as apoptosis-inducing agent.
Keywords
Chelidonium majus; breast cancer; MDA-MB-231; apoptosis; anti-proliferative;
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Times Cited By KSCI : 7  (Citation Analysis)
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