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Morphological and Cytogenetic Analysis of Colchicine-induced Tetraploids of Fallopia multiflolra Haraldson (Colchicine 처리에 의해 유기된 4배체 하수오의 형태 및 세포유전학적 특성)

  • Kim, Ki Hyun;Youn, Cheol Ku;Kim, In Jae;Lee, Kyung Ja;Kim, Young Ho;Hong, Seong Tack;Woo, Sun Hee
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.5
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    • pp.362-369
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    • 2018
  • Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

Advanced Field Weakening Control for Maximum Output Power Operation of Induction Motor in a Limited Environment

  • Seo, Yong-Joo;Go, Hee-Young;Kim, Jang-Mok
    • Proceedings of the KIPE Conference
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    • 2012.11a
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    • pp.217-218
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    • 2012
  • A load motor used for warship or submarine is with limited volume and weight, also specific environmental tests like impact, vibration, noise, temperature and EMC/EMI have to be satisfied. Induction motors, synchronous motors, BLDC motor and etc, are used depending on the purpose of using military equipment. Induction Motors are used for a number of military equipment more commonly due to the robust structure and simple maintenance. Domestic and foreign warships have a wide range of voltages as the DC voltage sources with battery are mainly used for them. The ${\Delta}-connection$ operation of the induction motor is required to make the maximum power in a low voltage level. But the elements' temperature of the inverter increases due to high input current when it is in the ${\Delta}-connection$ operation. Therefore, the induction motor must be driven with the Y-connection. The lack of voltage needs to be with the field weakening control. This paper suggests the optimum field weakening control algorithm to drive the induction motor with maximum power in a limited thermal and DC voltage condition.

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Integration of Heterogeneous Models with Knowledge Consolidation (지식 결합을 이용한 서로 다른 모델들의 통합)

  • Bae, Jae-Kwon;Kim, Jin-Hwa
    • Korean Management Science Review
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    • v.24 no.2
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    • pp.177-196
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    • 2007
  • For better predictions and classifications in customer recommendation, this study proposes an integrative model that efficiently combines the currently-in-use statistical and artificial intelligence models. In particular, by integrating the models such as Association Rule, Frequency Matrix, and Rule Induction, this study suggests an integrative prediction model. Integrated models consist of four models: ASFM model which combines Association Rule(A) and Frequency Matrix(B), ASRI model which combines Association Rule(A) and Rule Induction(C), FMRI model which combines Frequency Matrix(B) and Rule Induction(C), and ASFMRI model which combines Association Rule(A), Frequency Matrix(B), and Rule Induction(C). The data set for the tests is collected from a convenience store G, which is the number one in its brand in S. Korea. This data set contains sales information on customer transactions from September 1, 2005 to December 7, 2005. About 1,000 transactions are selected for a specific item. Using this data set. it suggests an integrated model predicting whether a customer buys or not buys a specific product for target marketing strategy. The performance of integrated model is compared with that of other models. The results from the experiments show that the performance of integrated model is superior to that of all other models such as Association Rule, Frequency Matrix, and Rule Induction.

Development of Induction Heating Apparatus for Rapid Heating of Metallic Mold (미세 임프린팅용 금속몰드의 급속가열을 위한 유도가열기구 개발)

  • Hong, S.K.;Lee, S.H.;Heo, Y.M.;Kang, J.J.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2007.05a
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    • pp.199-204
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    • 2007
  • Hot embossing, one of Nanoimprint Lithography(NIL) techniques, has been getting attention as an alternative candidate of next generation patterning technologies by the advantages of simplicity and low cost compared to conventional photolithographies. A typical hot embossing usually, however, takes more than ten minutes for one cycle of the process because of a long thermal cycling. Over the last few years a number of studies have been made to reduce the cycle time for hot embossing or similar patterning processes. The target of this research is to develop an induction heating apparatus for heating a metallic micro patterning mold at very high speed with the large-area uniformity of temperature distribution. It was found that a 0.5 mm-thick nickel mold can be heated from $25^{\circ}C$ to $150^{\circ}C$ within 1.5 seconds with the temperature variation of ${\pm}5^{\circ}C$ in 4-inch diameter area, using the induction heating apparatus.

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Effects of Intermediate Metabolites on Phenanthrene Biodegradation

  • Cho Hwa-Young;Woo Seung-Han;Park Jong-Moon
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.969-973
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    • 2006
  • Stimulatory effects of three different intermediate metabolites (1-hydroxy-2-naphthoate, salicylate, and catechol) as potential inducers on phenanthrene degradation were investigated using two different bacteria (Pseudomonas putida ATCC 17484 and Burkholderia cepacia PB12). The relative induction capacity was high in the sequence of 1-hydroxy-2-naphthoate, salicylate, and catechol in both strains. The highest of up to 12 times increase of the induction was obtained by the addition of 1-hydroxy-2-naphthoate in the strain PB12, compared with the control where no exogenous inducer was added. The induction capacity of the potential inducers was closely related with the number of oxygenations required per electron equivalents in one mole of the inducer.

The Comparative Analysis of 2-Phase Inverter Topologies for 2-Phase Induction Motor (2상 유도전동기 구동을 위한 2상 인버터 토폴로지의 비교 분석)

  • Kim, Dong-Ki;Yoon, Duck-Yong
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.6
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    • pp.1013-1018
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    • 2016
  • In this paper, three kinds of the inverter topologies for the variable speed drive of 2-phase induction motor are compared and analyzed. The 2-phase inverters are classified into 2-leg, 3-leg, and 4-leg types depending on the number of power switching devices. And they use the output voltage vectors of the different forms according to the inverter topologies. Based on the comparative analyzed results, the effective values of output voltage have been defined by the linear modulation range. Therefore, the motor design guideline is proposed in order to decide a rated voltage of 2-phase induction motor according to the inverter topologies. Also, the computer simulations are carried out to verify the output voltage and current characteristics of each inverter topology.

Analysis of the torque transient performance of the induction motor by means of phase segregation method (상분리법에 의한 유도전동기의 토오크 과도특성해석)

  • Jeong, Jong-Ho;Lee, Eun-Woong;Choi, Jae-Young
    • Proceedings of the KIEE Conference
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    • 2000.11b
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    • pp.247-249
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    • 2000
  • Transient phenomena cause delay in control response and must be studied and eliminated, if possible, suppressed. The difficulty in analyzing transient phenomena in ac machines comes from the large number of windings involved. But, it is possible that only one phase is used to represent three phases of the induction motor as called phase segregation method. The phase segregation method provides equivalent circuits for both the steady and transient states of induction motor. In this paper, analysis of the torque transient of the induction motor be carried out the phase segregation method and confirmed in the possibility of transientless torque control.

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Coil Design of Pulse Induction Metal Detector (펄스 유도 방식의 금속탐지기 코일 설계)

  • Jung, Byung-Min;Chang, Yu-Shin;Han, Seung-Hoon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.26 no.4
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    • pp.389-396
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    • 2015
  • A coil design of pulse induction metal detectors has been described. The search coil was demonstrated by using the wire with the diameter of 0.3 mm, 0.5 mm and 1.0 mm and the dielectric plate with the $30cm{\times}30cm$ and $35cm{\times}35cm$, the time constant and the currents of the coil as the variation of the coil size and the number of coil turns was characterized. The coil parameters like the resistance, the inductance and the time constants as the variation of the diameter of the wire, the coil size and the number of coil turns were compared and analysed through the calculation and the measurement. In addition, investigating the coil currents as the variation of the input pulse width, the coil design of pulse induction metal detectors has been discussed.

Machine Learning Perspective Gene Optimization for Efficient Induction Machine Design

  • Selvam, Ponmurugan Panneer;Narayanan, Rengarajan
    • Journal of Electrical Engineering and Technology
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    • v.13 no.3
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    • pp.1202-1211
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    • 2018
  • In this paper, induction machine operation efficiency and torque is improved using Machine Learning based Gene Optimization (ML-GO) Technique is introduced. Optimized Genetic Algorithm (OGA) is used to select the optimal induction machine data. In OGA, selection, crossover and mutation process is carried out to find the optimal electrical machine data for induction machine design. Initially, many number of induction machine data are given as input for OGA. Then, fitness value is calculated for all induction machine data to find whether the criterion is satisfied or not through fitness function (i.e., objective function such as starting to full load torque ratio, rotor current, power factor and maximum flux density of stator and rotor teeth). When the criterion is not satisfied, annealed selection approach in OGA is used to move the selection criteria from exploration to exploitation to attain the optimal solution (i.e., efficient machine data). After the selection process, two point crossovers is carried out to select two crossover points within a chromosomes (i.e., design variables) and then swaps two parent's chromosomes for producing two new offspring. Finally, Adaptive Levy Mutation is used in OGA to select any value in random manner and gets mutated to obtain the optimal value. This process gets iterated till finding the optimal value for induction machine design. Experimental evaluation of ML-GO technique is carried out with performance metrics such as torque, rotor current, induction machine operation efficiency and rotor power factor compared to the state-of-the-art works.

HIF-1α-Dependent Gene Expression Program During the Nucleic Acid-Triggered Antiviral Innate Immune Responses

  • Hong, Sun Woo;Yoo, Jae Wook;Kang, Hye Suk;Kim, Soyoun;Lee, Dong-ki
    • Molecules and Cells
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    • v.27 no.2
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    • pp.243-250
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    • 2009
  • Recent studies suggest a novel role of $HIF-1{\alpha}$ under nonhypoxic conditions, including antibacterial and antiviral innate immune responses. However, the identity of the pathogen-associated molecular pattern which triggers $HIF-1{\alpha}$ activation during the antiviral response remains to be identified. Here, we demonstrate that cellular administration of double-stranded nucleic acids, the molecular mimics of viral genomes, results in the induction of $HIF-1{\alpha}$ protein level as well as the increase in $HIF-1{\alpha}$ target gene expression. Whole-genome DNA microarray analysis revealed that double-stranded nucleic acid treatment triggers induction of a number of hypoxia-inducible genes, and induction of these genes are compromised upon siRNA-mediated $HIF-1{\alpha}$ knock-down. Interestingly, $HIF-1{\alpha}$ knock-down also resulted in down-regulation of a number of genes involved in antiviral innate immune responses. Our study demonstrates that $HIF-1{\alpha}$ activation upon nucleic acid-triggered antiviral innate immune responses plays an important role in regulation of genes involved in not only hypoxic response, but also immune response.