• Natural Product Sciences
• /
• 제21권3호
• /
• pp.205-209
• /
• 2015

Fucoidan, a sulfated polysaccharide is found in several types of edible brown algae. It has shown numerous biological activities; however, the molecular mechanisms on the activity against atopic dermatitis have not been reported yet. We now examined the effects of fucoidan on chemokine production co-induced by TNF-α/IFN-γ, and the possible mechanisms underlying these biological effects. Our data showed that fucoidan inhibited the TNF-α/IFN-γ-induced production of thymus and activation-regulated chemokine (TARC) and macrophagederived chemokine (MDC) mRNA in human keratinocytes HaCaT cells. Also, fucoidan suppressed phosphorylation of nuclear factor kappa B (NF-κB) and activation of signal transducer and activator of transcription (STAT)1 in a dose-dependent manner. In addition, fucoidan significantly inhibited activation of extracellular-signal-regulated kinases (ERK) phosphorylation. These data indicate that fucoidan shows anti-inflammatory effects by suppressing the expression of TNF-α/IFN-γ-induced chemokines by blocking NF-κB, STAT1, and ERK1/2 activation, suggestive of as used as a therapeutic application in inflammatory skin diseases, such as atopic dermatitis.

• 전기학회논문지P
• /
• 제57권2호
• /
• pp.79-83
• /
• 2008

In this paper, we introduced a LIF measurement method and summarized the theoretical side. When an altered wavelength of laser and electric power, lamp applied electric power, we measured the relative density of the metastable state in mercury after observing a laser induced fluorescence signal of 404.8nm and 546.2nm, and confirmed the horizontal distribution of plasma density in the discharge lamp. Due to this generation, the extinction of atoms in a metastable state occurred through collision, ionization, and excitation between plasma particles. The density and distribution of the metastable state depended on the energy and density of plasma particles, intensely. This highlights the importance of measuring density distribution in plasma electric discharge mechanism study. The results confirmed the resonance phenomenon regarding the energy level of atoms along a wavelength. change, and also confirmed that the largest fluorescent signal in 436nm, and that the density of atoms in 546.2nm ($6^3S_1{\to}6^3P_2$) were larger than 404.8nm ($6^3S_1{\to}6^3P_2$). According to the increase of lamp applied electric power, plasma density increased, too. When increased with laser electric power, the LIF signal reached a saturation state in more than 2.6mJ. When partial plasma density distribution along a horizontal axis was measured using the laser induced fluorescence method, the density decreased by recombination away from the center.

• 대한기계학회논문집B
• /
• 제24권5호
• /
• pp.725-732
• /
• 2000

Laser induced incandescence (LII) method is frequently used to measure soot volume fraction in flames. In this study, experiments were performed to measure soot volume fraction in coaxial diffusion flame using LII method and calibrated with laser scattering/extinction method. The effects of laser intensity (>$1{\times}10^8W/cm^2$), laser wavelength (532nm, 1064nm) and detection wavelength (400nm, 600nm) on the LII signal were investigated. On the range of $4{\times}10^8{\sim}8{\times}10^8W/cm^2$ there were no effects of laser intensity on LII signal. Except these ranges, LII signal was increased with laser intensity. For the long gate width, the LII signals of the higher laser intensity (>${\vartheta}(GW/cm^2)$) cases had better correlation with soot volume fraction which were measured by laser extinction method compared with lower laser intensity cases. The errors of 2-dimensional cases at the calibration height were approximately 50% regardless of laser wavelength.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
• /
• pp.570-571
• /
• 2005

In this paper, we introduced a LIF measurement method and summarized the theoretical side. When an altered wavelength of laser and electric power, lamp applied electric power, we measured the relative density of the metastable state in mercury after observing a laser induced fluorescence signal of 404.8nm and 546.2nm, and confirmed the horizontal distribution of plasma density in the discharge lamp. The results confirmed the resonance phenomenon regarding the energy level of atoms along a wavelength change, and also confirmed that the largest fluorescent signal in 436nm, and that the density of atoms in 546.2nm ($6^3S_1\rightarrow6^3P_2$) were larger than 404.8nm ($6^3S_1\rightarrow6^3P_1$). According to the increase of lamp applied electric power, plasma density increased, too. When increased with laser electric power, the L1F signal reached a saturation state in more than 2.6mJ. When partial plasma density distribution along a horizontal axis was measured using the laser induced fluorescence method, the density decreased by recombination away from the center.

• IMMUNE NETWORK
• /
• 제5권1호
• /
• pp.30-35
• /
• 2005

Background: p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) signaling are thought to have critical role in lipopolysaccharide (LPS)-induced immune response but the molecular mechanism underlying the induction of these signaling are not clear. Methods: Specific inhibitors for p38, SB203580, and for ERK, PD98059 were used. Cells were stimulated by LPS with or without specific MAPK inhibitors. Results: LPS activated inducible nitric oxide synthase (iNOS), subsequent NO productions, and pro-inflammatory cytokine gene expressions (TNF-${\alpha}$, IL-$1{\beta}$, IL-6, and IL-12). Treatment of both SB203580 and PD98059 decreased LPS-induced NO productions. Concomitant decreases in the expression of iNOS mRNA and protein were detected. SB203580 and PD98059 decreased LPS-induced gene expression of IL-$1{\beta}$ and IL-6. SB203580 increased LPS-induced expression of TNF-${\alpha}$ and IL-12, and reactive oxygen species production, but PD98059 had no effect. Conclusion: These results indicate that both p38 and ERK pathways are involved in LPS-stimulated NO synthesis, and expression of IL-$1{\beta}$ and IL-6. p38 signaling pathways are involved in LPS-induced TNF-${\alpha}$ and IL-12, and reactive oxygen species plays an important role in these signaling in macrophage.

• 한국광학회지
• /
• 제10권1호
• /
• pp.47-52
• /
• 1999

두 개의 짧은 광경로가 밀착된 마하젠더 간섭계를 사용한 보다 간단하고 효율적인 시럼방법을 통하여 펌프광에 의해 유도되는 어븀 첨가 광섬유의 굴절률 변화와 분산특성을 측정하엿으며, 어븀 첨가 농도와 펌프광 세기에 이들 값들의 변화 특성을 규명하였다. 어븀 이옹의 첨가 농도가 300ppm에서 700ppm으로 증가할 때 1510∼1560nm의 파장 범위에서 1530 nm 의 공명파장 전후로 광유도된 굴절률과 분산의 변화량은 거의 선형적으로 증가하며 펌프광의 세기가 커질수록 변화량들은 증가하다가 전자들이 전부 여기됨과 동시에 점차 포화되는 것을 알 수 있었다.

• Journal of Audiology & Otology
• /
• 제24권4호
• /
• pp.174-179
• /
• 2020

Background and Objectives: The integration of auditory-visual speech information improves speech perception; however, if the auditory system input is disrupted due to hearing loss, auditory and visual inputs cannot be fully integrated. Additionally, temporal coincidence of auditory and visual input is a significantly important factor in integrating the input of these two senses. Time delayed acoustic pathway caused by the signal passing through digital signal processing. Therefore, this study aimed to investigate the effects of hearing loss and hearing aid digital delay circuit on sound-induced flash illusion. Subjects and Methods: A total of 13 adults with normal hearing, 13 with mild to moderate hearing loss, and 13 with moderate to severe hearing loss were enrolled in this study. Subsequently, the sound-induced flash illusion test was conducted, and the results were analyzed. Results: The results showed that hearing aid digital delay and hearing loss had no detrimental effect on sound-induced flash illusion. Conclusions: Transmission velocity and neural transduction rate of the auditory inputs decreased in patients with hearing loss. Hence, the integrating auditory and visual sensory cannot be combined completely. Although the transmission rate of the auditory sense input was approximately normal when the hearing aid was prescribed. Thus, it can be concluded that the processing delay in the hearing aid circuit is insufficient to disrupt the integration of auditory and visual information.

• 한국지반환경공학회 논문집
• /
• 제23권10호
• /
• pp.13-19
• /
• 2022

본 연구는 암석의 압축강도를 비파괴적으로 산정하기 위하여 암석시편 초기타격 및 반발에 의한 연속적인 반복타격 시 발생하는 타격력에 대한 응답신호를 모두 측정하고 이를 누적한 전체 타격력 신호에너지를 이용하고 그 결과를 제시하는 것에 관한 것이다. 본 연구에서는 이를 위해서 타격 및 측정장치를 고안 및 셋업하였고 이를 이용하여 암석시편을 회전 자유낙하에 의해 초기 타격토록하고 반발작용에 의한 반복타격이 이루어질 수 있도록 하였다. 본 연구에서는 서로 다른 세 종류의 암석시편에 대하여 타격력실험을 실시하고 발생신호를 측정하였다. 각 시편별 초기 및 반발타격으로부터 발생된 신호로부터 산정된 전체 타격력 신호에너지와 각 시편별 측정한 직접압축강도와 상호 비교하였다. 비교결과, 타격력 응답신호로 부터 산정된 전체 타격력 신호에너지는 시편의 직접압축강도와 직접적인 관계가 있다는 것을 확인하였으며, 이를 통해 암석의 압축강도는 타격 시 발생하는 타격력 응답신호로부터 산정된 전체 타격력 신호에너지를 이용하여 비파괴적으로 산정할 수 있음을 알 수 있었다.

• 대한통합의학회지
• /
• 제11권2호
• /
• pp.169-179
• /
• 2023

Purpose : Although human periodontal ligament stem cells (hPDLSCs) are a supportive factor for tissue engineering, oxidative stress during cell culture and transplantation has been shown to affect stem cell viability and mortality, leading to failed regeneration. The aim of this study was to evaluate the antioxidant and protective effects against cell damage of celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, and the antioxidant signal of hPDLSCs in H₂O₂-induced oxidative stress. Methods : To induce oxidative stress in cultured hPDLSCs, H₂O₂ was used as an exogenous reactive oxygen species (ROS). Dose-dependent celecoxib (.1, 1, 10, or 100 µM) was administered after H₂O₂ treatment. WST-1 assay was used to assess cell damage and western blot was used to observe antioxidant activity of hPDLSCs in oxidative stress. Immunohistochemistry was performed for inverting the localization of the SOD and Nrf2 antibody. Results : We found that progressive cell death was induced in hPDLSCs by H₂O₂ treatment. However, low-dose celecoxib reduced H₂O₂-induced cellular damage and eventually enhanced the SOD activity and Nrf2 signal of hPDLSCs. Oxidative stress-induced morphological change in hPDLSCs included lowered the survival and number of spindle-shaped cells, and shrinkage and shortening of cell fibers. Notably, celecoxib promoted cell survival function and activated antioxidants such as SOD and Nrf2 by positively regulating the cell survival signal pathway, and also reduced the number of morphological changes in hPDLS. Immunohistochemistry results showed a greater number of SOD- and Nrf2-stained cells in the celecoxib-treated group following oxidative stress. Conclusion : By increasing SOD and Nrf2 expression at the antioxidant system, the findings suggest that celecoxib enhanced the antioxidative ability of hPDLSCs and protected cell viability against H₂O₂-induced oxidative stress by increasing SOD and Nrf2 expression in the antioxidant system.

• 한국복합재료학회:학술대회논문집
• /
• 한국복합재료학회 2005년도 춘계학술발표대회 논문집
• /
• pp.235-239
• /
• 2005

In this research, we investigated the frequency characteristic of low-velocity impact induced damage signals on graphite/epoxy composite laminates using high-speed fiber Bragg grating(FBG) sensor system. Appling the FBG sensors to damage assessment, we need to study the response of FBG sensors as the damage signals of the different incident angles because FBG shows different directional sensitivity. In order to discriminate an impact induced damage signal from that of undamaged case, drop impacts with different energies were applied to the composite panel with different incident angle to the FBG sensor. Finally, detected impact signals were compared using frequency distributions of wavelet detail components in order to find distinctive signal characteristics of composites delamination.