• Journal of Microbiology and Biotechnology
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• v.10 no.1
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• pp.56-62
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• 2000

The hpa gene genetically linked to the ctxa2b gene was cloned into the pTED expression vector, and the constructed pTEDhpa/ctxa2b was transformed into Excherichia coli. The fusion protein, the adhesin fused to the cholera toxin subunit A2B (CTXA2B) subunit, was expressed to high levels as inclusion bodies in E. coli. The expressed protein was partially purified by washing the inclusion bodies with working solution containing 8M Urea and 0.1M DTT. Refolding of denatured fusion protein was carried out in the presence of glutathione redox buffer. The refolded fusion protein was purified by size exclusion chromatography. The expressed fusion protein was verified by SDS-PAGE, western blotting with antibodies to both antigenic components of adhesin and cholera toxin subunit B (CTXB), and its N-terminal amino acid sequence was analyzed. The orderly assembled fusion protein was confirmed by modified Gm1-ganglioside ELISA with Abs to adhesin. The results indicate that the purified fusion protein is an Adhesin/CTXA2B protein containing the H. pylori adhesin and $G_{m1}4-ganglioside binding activity of CTXB and the expressed fusion protein in E. coli could be easily purified by the refolding process, Its molecular weight was 168kDa as estimated by size exclusion chromatography. The Adhesin/CTXA2B protein may be used as a candidate antigen for oral immunization against H. pylori.

• The Journal of Internal Korean Medicine
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• v.37 no.1
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• pp.109-134
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• 2016

Objectives: The aim of this study was to help develop a guideline for the common cold. We searched recent clinical studies of the common cold in Western medicine and reviewed their objectives, inclusion and exclusion criteria, primary outcome, secondary outcome, and assessment tools to establish evidenced-based guideline.Methods: We searched electronic databases (Cochrane Library, MEDLINE, EMBASE) to identify eligible randomized controlled trials (RCTs) about the common cold for the last 10 years. We included 29 RCTs and showed their research summary via their objectives, participants, interventions, control, treatment duration, and results. We also analyzed the definition of the common cold presented in the article, inclusion and exclusion criteria, primary and secondary outcomes, and assessment tools.Results: We reported the aforementioned areas in detail. At first, the definition of the common cold was confused across the articles. Second, herbal medication clinical trials for the common cold have been extensively studied recently. Third, the eligibility criteria frequently included the Jackson Symptom score. Fourth, validated assessment tools (i.e., the Wisconsin Upper Respiratory Symptom Survey-21) have only been used in a few recent studies.Conclusions: Our research will be helpful to establish Korean herbal medicine clinical trial guidelines for the common cold.

• Journal of Korean Academic Society of Home Health Care Nursing
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• v.21 no.1
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• pp.14-25
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• 2014

Purpose: The purpose of this study was to analyze key performance indicators using a balanced scorecard in the nursing department of hospitals, and to find useful suggestions for future research. Methods: Before starting the literature search clear inclusion and exclusion criteria were determined. After that, were viewed 41 studies from the RISS, KISS, National assembly library, and NDSLDB. Data were collected on October 17, 2013. Researchers developed an analytic framework for the literature review using a balanced score card development process and construction steps of previous studies. This framework had 10 steps related to the balanced score card development process. Results: A total of 10 studies met the inclusion and exclusion criteria. We identified 234 key performance indicators for the nursing department. The average number was 6.6 in finance perspective, 8.6 in perspective, 11.9 in internal process perspective, and 6.2 in learning and growth perspective. We found that 173 indicators had been cited only once. 61 indicators were cited over twice. Further, 8 indicators employed different perspectives on different studies. Conclusion: The results of the present study summarize useful information on recent trends on key performance indicators using a balanced score card in the nursing department of hospitals. These results will help develop key performance indicators and provide directions for future research.

• Health Policy and Management
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• v.20 no.4
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• pp.45-57
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• 2010

Background : If different cost efficiency indexes were informed to the same clinic depending on the inclusion or exclusion of pharmacy cost, it may impair the reliability of provider-profiling system. This study aimed to investigate whether the omission of pharmacy cost affects cost-efficiency rankings in medical clinics. Methods : Data for ambulatory care cost at 23,112 medical clinics were collected from the claims database, which was constructed after review by the Health Insurance Review and Assessment Service (HIRA) of Korea in April 2007. We calculated two types of cost efficiency indexes by inclusion or exclusion of pharmacy cost for a medical clinic. The agreement between the decile rankings of the two indexes was also assessed using the weighted kappa statistic of Landis and Koch. Results : When the cost efficiency index for total cost including pharmacy cost was compared with the index for total cost excluding it, the agreement between the two indexes was only 55%. The agreements between the two indexes were relatively low within specialties which have larger pharmacy volume of total cost and lower correlation between total cost with or without pharmacy cost included than the average level of all the specialties. Conclusion : These results suggest that the omission of pharmacy cost may result in contradictory outcomes that may be confusing to a medical institution and may impair the reliability of provider-profiling systems. It is very important to standardize profiling criteria for the reliability of provider profiling system.

• Nuclear Engineering and Technology
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• v.24 no.1
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• pp.14-29
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• 1992

COMCAF, a computer code for the common-cause failure analysis, is developed to treat the common-cause failures in nuclear power plants. In the treatment of common-cause failures, the minimal cut sets of the system are obtained first without changing the fault-tree structure. The occurrence probabilities of the minimal cut sets are then calculated accounting for the common-cause failures among components in the same minimal cut set or in different minimal cut sets. The basic parameter model is used to model the common-cause failures between similar or identical components. For dissimilar components, the assumption of symmetry used in the basic parameter model is applied to the basic events affecting two or more components. The top event probability is evaluated using the inclusion-exclusion method. In addition to the common-cause failures of components in the same minimal cut sets, failures of components in the different minimal cut sets are also easily accounted for by this method. This study applied this common-cause failure analysis to the PWR auxiliary feedwater system. The results in the top event probability for the system are compared with those of no common-cause failures.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.3
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• pp.93-101
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• 2013

This paper suggests optimal location algorithm of new firm $A(F_A)^{\prime}s$ p(p$B(F_B)$ already operating q outlets of sports in the market. This algorithm selects top q nodes among $V=V{\backslash}F_B$ nodes that covers maximum nodes based on the shortest distance. Then, q nodes choose next node that has a maximum cover with inclusion-exclusion principle. At the time of same number of cardinality in q sets to pre-defined q, we select the maximum cover node set. This algorithm called by competitive algorithm. The competitive algorithm simply decides the optimal location of the outlets p=1,2,3,4 for q=5. Also, we show that the market share of competitive algorithm can be maximize.

• Journal of Agricultural Extension & Community Development
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• v.21 no.3
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• pp.91-119
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• 2014

This study explored feasibility of social cooperatives as a strategy of struggle to social exclusion in the social services area based on the relationships between people. Is social cooperatives on the Fundamental Act on Cooperatives a new type of social welfare service providers comparing the social welfare foundations and social enterprises? Would be expected as social inclusion processes effects arising from the properties of cooperative in social welfare sector? If social welfare institutions function as an intermediate medium between government and users, social cooperatives can be performed function in the community in a variety of cooperative relationships with Multiple stakeholders, e.g. various service providers, care-givers, social workers, and users, etc., conflicting the interests. In this perspective, looked the possibility of social cooperatives as community welfare practice model.

• Journal of the Korean Association of Geographic Information Studies
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• v.11 no.1
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• pp.20-30
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• 2008

This study is to analyse the hydrological behavior of agricultural reservoir using SWAT model. For the upsteam watershed of Gongdo water level gauge station in Anseongcheon watershed, the streamflows at 2 reservoir (Gosam and Geumgwang) locations and Gongdo station were simulated with reservoir inclusion and exclusion. The daily water surface area and storage have been calculated considering the stage-storage curve function of the reservoir. Afterwards, the reservoir operation module in SWAT was modified from original module in SWAT for daily reservoir discharge simulated by water balance equation. Model validation results were Nash-Sutcliffe model efficiency coefficients value of 0.55, root mean square error value of 2.33 mm/day. On the other hand, the simulation results of two reservoir exclusion were Nash-Sutcliffe model efficiency coefficients value of 0.37, root mean square error value of 2.91 mm/day. The difference of Nash-Sutcliffe model efficiency coefficients between the simulation results of two reservoir inclusion and exclusion at Gongdo station was 0.18. This is caused by the storage and release operation of agricultural reservoirs for the runoff occurred at 2 reservoir watersheds.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1620-1627
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• 2009

The design and expression of an antihypertensive peptide multimer (AHPM), a common precursor of 11 kinds of antihypertensive peptides (AHPs) tandemly linked up according to the restriction sites of gastrointestinal proteases, were explored. The DNA fragment encoding the AHPM was chemically synthesized and cloned into expression vector pGEX-3X. After an optimum induction with IPTG, the recombinant AHPM fused with glutathione S-transferase (GST-AHPM) was expressed mostly as inclusion body in Escherichia coli BL21 and reached the maximal production, 35% of total intracellular protein. The inclusion body was washed, dissolved, and purified by cation-exchange chromatography under denaturing conditions, followed by refolding together with size-exclusion chromatography and gradual dialysis. The resulting yield of the soluble GSTAHPM (34 kDa) with a purity of 95% reached 399 mg/l culture. The release of high active fragments from the AHPM was confirmed by the simulated gastrointestinal digestion. The results suggest that the design strategy and production method of the AHPM will be useful to obtain a large quantity of recombinant AHPs at a low cost.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.522-525
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• 2002

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.