• 제목/요약/키워드: inbred lines

검색결과 238건 처리시간 0.019초

Development of Clubroot Resistant Doubled-Haploid Inbred Lines in Kimchi Cabbage (Chinese Cabbage) (Brassica rapa L.)

  • Park, Suhyoung;Jang, Hayoung;Park, Min Young
    • 한국균학회소식:학술대회논문집
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    • 한국균학회 2015년도 춘계학술대회 및 임시총회
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    • pp.37-37
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    • 2015
  • Kimchi cabbage (Chinese cabbage), radish and Cabbage are major Brassicaceae vegetables in Korea. Especially, we can easily develop whole plant from one microspore in Kimchi cabbage. To develop clubroot resistant doubled-haploid (DH) inbred lines, we pollinated a clubroot resistant turnip of 'IT 033820' with a Kimchi cabbage (Chinese cabbage) inbred of 'BP 079'. More than 85 DH inbred lines were developed from this combination. We screened about 400 materials including these DH inbred lines, commercial cultivars and breeding materials during 3 years using hydroponic system after inoculating single spore isolation race 4(SSI-04) inoculate. One inbred line derived from this combination selected as clubroot resistant and registered as 'Wonkyo20036ho'. We inoculated 26 DH inbred lines derived from 'Zoong-baek 2ho' using SSI-4, the percent of resistant plants varied from 0 to 83%. However the horticultural traits of highly resistant DH inbred line was poor. Thus we selected one DH line showing 77% resistant with yellow inner leaf and maid good head, was registered as 'Wonkyo20034ho'. Another DH inbred line derived from Korean variety of 'Wol-dong' showing 86% resistant was registered as 'Wonkyo20037ho'. Other DH inbred lines were derived from Chinese cultivar of 'Choon-hi-go-hang-wang' and 'Hwang-shim-zo48' showed 80 and 71% resistant, respectively, was also selected for registration. Even though DH inbred lines derived from turnip showed highly resistant to SSI-04 and provincial inoculate, they showed poor characteristics in horticultural traits. However, commercial seed companies showed interesting for adapting these DH inbred lines in commercial breeding.

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SSR 분자마커를 이용한 찰옥수수 및 종실용 옥수수 자식계통들의 핵심집단에 대한 유전적 다양성 및 집단구조 분석 (Analysis of Genetic Diversity and Population Structure for Core Set of Waxy and Normal Maize Inbred Lines using SSR Markers)

  • 사규진;김진아;박기진;박종열;고병대;이주경
    • 한국육종학회지
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    • 제43권5호
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    • pp.430-441
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    • 2011
  • 본 연구는 총 50개의 SSR 마커를 이용하여, 찰옥수수 및 종실용 옥수수 핵심집단(찰옥수수 40계통, 종실용 옥수수 40계통)의 자식계통들의 유전적 다양성, 집단구조 및 계통유연관계를 분석하였다. 1. 그 결과 65bp에서 225bp 크기의 범위로 총 242개의 대립단편들을 증폭시켰다. SSR primer들에서 증폭된 대립단편의 수는 최소 2개에서부터 최대 9개까지의 범위로 나타났고, 평균 4.84개가 증폭되었다. 그리고 GD값은 0.420에서 0.854의 범위로 나타났고, 평균 0.654의 값을 나타내었다. 2. 80개의 옥수수 자식계통들의 집단구조를 분석한 결과, 13개의 찰옥수수 자식계통은 group I에 포함되었고, Group II는 7개의 찰옥수수 자식계통과 38개의 종실용 옥수수 자식계통들이 포함되었다. 나머지 22개의 자식계통들은 admixed group에 포함되었으며, 20개 찰옥수수 자식계통과 2개의 종실용 옥수수 자식계통으로 구성되어있다. 3. UPGMA법에 의한 계통유연관계 분석 결과, 80개 옥수수 자식계통들은 유전적 유사성 31.7% 수준에서 크게 3개의 그룹으로 나누어졌다. Group I은 40개의 찰옥수수 자식계통과 11개의 종실용 옥수수 자식계통을 포함하고 있었고, Group II는 27개의 종실용 옥수수 자식계통을, 그리고 Group III은 단지 2개의 종실용 옥수수 자식계통을 포함하고 있었다. 따라서 본 연구의 결과는 앞으로 강원도 농업기술원 옥수수시험장에서 육성한 찰옥수수 및 종실용 옥수수 자식계통들에 대한 유전자원 관리 및 선발 그리고 교배조합 구성 및 예측 등에 유용한 정보를 제공할 것으로 기대된다.

Association of Marker Loci and QTL from Crosses of Inbred Parental Lines

  • Lee, Gi-Woong
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권6호
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    • pp.772-779
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    • 2005
  • The objectives of this study were to examine problems with using F$_1$ data by simulation, association of marker loci and QTL from crosses of inbred parental lines and to enumerate the preliminary characterization of genetic superiority within inbred parental lines. In this study, the association between markers for QTL used as covariates and estimates of variance components due to effects of lines was investigated through computer simulation. The effects of size of population to develop inbred lines and initial frequencies and magnitudes of effects of QTL were also considered. Results show that estimates of variance components due to line effects are influenced by including marker information as covariates in the model for analysis. Estimates of line variance were increased by adding marker information into the analysis, because negative covariances between effects associated with the markers and the remaining effects associated with other loci existed. However, the fit of the model as indicated by the log likelihood improved by adding more markers as covariates into the analysis. Marker assisted selection will be beneficial when markers explain unexplained genetic difference during selection procedure. Markers can be used to identify QTLs affecting traits, and to select for favorable QTL alleles. To efficiently use genetic markers, location of markers at the genome must be identified. The estimates of variance due to effects of with and without marker information used as covariates in the analysis were investigated. The estimates of line variances were always increased when markers were included as covariates for the model because a negative covariance were existed.

R Type-S Block Designs for Control versus Test Inbred Lines Comparisons for Diallel Crosses

  • Son, Young-Nam
    • Communications for Statistical Applications and Methods
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    • 제9권2호
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    • pp.443-450
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    • 2002
  • In this paper, block designs for control versus test comparisons among the lines for diallel crosses are proposed. These block designs are constructed by using balanced incomplete block designs. Also, the efficiencies of the diallel crosses block designs obtained through this method are tabulated for number of lines 23 or less.

Fruit Yield and Quality Evaluation of Sweet Pepper (Capsicum annuum L.) F1 Hybrids Derived from Inbred Lines

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • 한국육종학회지
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    • 제42권4호
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    • pp.344-350
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    • 2010
  • Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce $F_1$ hybrids. A total of 37 $F_1$ hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the $F_1$ hybrids. Superiority on yield over standard/commercial varieties were differed among $F_1$ hybrids. Hybrid $5AVS8{\times}SP45$ exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the $F_1$ hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, $KNU1017{\times}SP27$, $5AVS1{\times}SP43$, $5AVS5{\times}SP27$, $5AVS8{\times}SP45$, $SP12{\times}SP38$ and $SP27{\times}SP25$ hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce $F_1$ hybrid seed for commercial production.

Expression of Flowering-Related Genes in Two Inbred Lines of Chinese Cabbage

  • Jang Hyun-Seung;Lim Yong-Pyo;Hur Yoon-Kang
    • Journal of Plant Biotechnology
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    • 제5권4호
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    • pp.209-214
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    • 2003
  • Expression of 43 flowering-related genes was examined in two inbred lines of Chinese cabbage, Chiifu and Kenshin, under different photoperiod, vernalization and flower development stages. The floral genes cloned by RT-PCR with degenerated primers showed high homology with Arabidopsis counterparts. Genes in two inbred lines, TOC, CRY1, CO, RGAL and GAl, were highly expressed under all tested conditions. However, expression of three genes was regulated by particular experimental conditions. The expression of LHY gene was predominant in Chiifu under the short-day conditions, whereas the expression of RGAL gene was influenced by vernalization in both inbred lines. Besides, the expression of NAP gene was induced by vernalization only in Chiifu. Most of the flower identity-related genes were expressed during flower development. The transcript level for several genes was not detected in this experiment.

Genetic Diversity and Association Analyses of Chinese Maize Inbred Lines Using SSR Markers

  • Vathana, Yin;Sa, Kyu Jin;Lim, Su Eun;Lee, Ju Kyong
    • Plant Breeding and Biotechnology
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    • 제7권3호
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    • pp.186-199
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    • 2019
  • We selected 68 Chinese maize inbred lines to understand the genetic diversity, population structure, and marker-trait associations for eight agronomic traits and 50 simple sequence repeats (SSRs) markers. In this study, effective traits, such as days of anthesis (DA), days of silking (DS), ear height (EH), plant to ear height ratio (ER), plant height (PH), and leaf width (LW) were divided into PC1 and PC2 by PCA analysis for maize inbred lines. Genetic diversity analysis revealed a total of 506 alleles at 50 SSR loci. The mean number of alleles per locus was 10.12. The averages of genetic diversity (GD) and polymorphic information content (PIC) values were 0.771 and 0.743, respectively. Based on a membership probability threshold of 0.80, the population structure revealed that the total inbred lines were divided into three major groups with one admixed group. A marker-trait association using Q + K MLM showed that nine SSR markers (bnlg1017, umc2041, umc2400, bnlg105, umc1229, umc1250, umc1066, umc2092, and umc1426) were related with seven agronomic traits. Among these SSR markers, eight SSR markers were associated with only one agronomic trait (DA, DS, ER, LL, LW, PH, and ST), whereas one SSR marker (umc1229) was associated with two agronomic traits (DA and ST). These results will help in optimizing the choice of inbred lines for cross combinations, as well as in selecting markers for further maize breeding programs.

Variation of Univariate Flow Karyotypes and Chromosomal DNA Contents in Maize (Zea mays L.)

  • Lee, Jai-Heon;Lee, Myoung-Hoon;Kim, Kyung-Je
    • 한국작물학회지
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    • 제43권2호
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    • pp.128-133
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    • 1998
  • Analyses of now karyotypes using different maize (Zea mays L.) inbred lines have been performed. The accumulation and isolation of high quality and quantity metaphase chromosomes from root tips can be achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synchronized maize root tips and analyzed with a now cytometry. The variations of experimental now karyotypes were detected among inbred lines in terms of the positions and/or the numbers of chromosome peaks. The 2C DNA amount among 8 inbred lines ranged from 5.09 to 5.52 pg. The variability of DNA content in maize chromosome 1 was 9.1 % ranging from 0.685 to 0.747 pg. The selection of appropriate maize lines is critical for sorting specific single chromosome types. At least five different chromosome types can be discriminated and sorted from five maize lines.

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종실옥수수 자식계통들에 대한 형태적 특성 연구 (Analysis of Morphological Characteristics for Normal Maize Inbred Lines)

  • 박종열;사규진;박기진;이주경
    • 한국작물학회지
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    • 제59권3호
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    • pp.312-318
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    • 2014
  • 본 연구는 강원도농업기술원 옥수수연구소에서 종실옥수수 품종개발을 위하여 육성한 156개의 자식계통들에 대하여 총 14개의 질적 및 양적 형질들을 이용하여 형태적 변이연구를 수행하였다. 3개의 질적 형질들의 조사 결과에서 웅수색(QL1)과 자수색(QL2)은 황색을 나타내는 계통(85계통, 84계통)들이 가장 많았고, 초형(QL3)은 대부분의 계통(105 계통)이 반직립형을 나타내었다. 반면에 11개의 양적 형질들에 대한 조사 결과에서 출웅일수(QN1)는 평균 $66.7{\pm}4.2$ 일을, 출사일수(QN2)는 평균 $72.8{\pm}5.8$일을, 간경(QN3)은 $16.2{\pm}2.6mm$를, 간장(QN4)은 평균 $16.2{\pm}2.6mm$를, 착수고(QN5)는 평균 $84.3{\pm}17.3cm$를, 백립중(QN6)은 평균 $24.4{\pm}4.4g$을, 이삭장(QN7)은 평균 $47.0{\pm}17.9cm$를, 착립장(QN8)은 평균 $40.7{\pm}15.7cm$을, 이삭경(QN9)은 평균 $14.2{\pm}4.7cm$를, 종실중(QN10)은 평균 $175.6{\pm}97.6kg$을, 종자의 수분함량은 평균 $10.0{\pm}0.3%$를 각각 나타내었다. 이상의 결과에 의하면 분석에 이용된 자식계통들 중에서 11개의 자식계통(00hf3, 00hf19, 00hf30, 00hf36, 02S8069, 02S8072, 02S8090, 02S8099, 05S10011, 06S8085-6, 07S8011)들은 조사된 5개의 수량 관련 형질들 중에서 3~4개 이상의 형질들에서 비교적 높은 특성을 나타내었다. 한편 주성분 분석결과에서는 분석에 이용된 14개의 질적 및 양적 형질들 중에서 이삭장(QN7), 착립장(QN8), 이삭경(QN9), 종실중(QN10)은 제 1 주성분에서 양의 방향에 크게 기여하였고, 반면에 제 2 주성분에서는 출웅일수(QN1), 출사일수(QN2), 간장(QN4), 착수고(QN5)은 음의 방향에 크게 기여하였다. 따라서 본 연구에서 제 1축 및 제 2축의 주성분에서 양의 방향과 음의 방향에 크게 기여한 형질들은 본 연구에서 분석에 이용한 156개의 종실 옥수수 자식계통들을 식별하는데 유용한 형질들인 것으로 생각되었다. 본 연구에서 156개의 종실옥수수 자식계통들에 대한 형태적 변이 및 주성분 분석의 결과는 앞으로 강원도농업기술원 옥수수연구소에서 종실옥수수 품종개발을 위한 계통 육성 및 교배조합 구성 등에 유용한 정보를 제공할 것으로 기대한다.

Investigation of Cytoplasmic Male Sterility and Development of Maintainer and Restorer Lines in Rye (Secale cereale L.)

  • Heo, Hwa-Young;Hong, Byung-Hee;Seong, Rak-Chun;Park, Moon-Woong
    • 한국작물학회지
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    • 제45권5호
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    • pp.347-351
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    • 2000
  • Rye has been a major winter forage crop in Korea. Varietal improvement of rye has been practiced either by hybrid or population breeding systems. Hybrid breeding offers important advantages over population breeding since it is normally a cross-pollinated crop. The hybrid breeding in rye has been possible since cytoplasmically inherited forms of male sterility (CMS) and corresponding nuclear restorer genes were found. The objectives of this research were to develop the maintainer and restorer lines of Korean inbred lines and to estimate the effect of 'Pampa' type of CMS cytoplasm on yield and its related characteristics. For easy discrimination of male-sterile status of plants, anther scoring and the restore index system in which seed-setting and pollen quantity of viability were taken into account were established. High significant correlation between pollen quantity and pollen viability was found. For "Pampa" cytoplasm, four of 14 Korean inbred lines tested turned out to be a maintainer but no restorer was found. But for "235b" CMS cytoplasm, seven inbred lines acted as complete restorers. The Korean inbred rye lines acted mainly as maintainers in "Pampa" cytoplasm but acted mainly as restorer in "235b" cytoplasm. The 'Pampa' cytoplasm inducing male sterility reduced cohn length and plant height and increased the number of tiller, so forage yield and grain yield were enhanced. However, heading date was slightly delayed compared to the normal cytoplasm.elayed compared to the normal cytoplasm.

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