Expression of Flowering-Related Genes in Two Inbred Lines of Chinese Cabbage

  • Jang Hyun-Seung (Genome Research Center, Chungnam National University) ;
  • Lim Yong-Pyo (Genome Research Center, Chungnam National University) ;
  • Hur Yoon-Kang (Genome Research Center, Chungnam National University)
  • Published : 2003.12.01

Abstract

Expression of 43 flowering-related genes was examined in two inbred lines of Chinese cabbage, Chiifu and Kenshin, under different photoperiod, vernalization and flower development stages. The floral genes cloned by RT-PCR with degenerated primers showed high homology with Arabidopsis counterparts. Genes in two inbred lines, TOC, CRY1, CO, RGAL and GAl, were highly expressed under all tested conditions. However, expression of three genes was regulated by particular experimental conditions. The expression of LHY gene was predominant in Chiifu under the short-day conditions, whereas the expression of RGAL gene was influenced by vernalization in both inbred lines. Besides, the expression of NAP gene was induced by vernalization only in Chiifu. Most of the flower identity-related genes were expressed during flower development. The transcript level for several genes was not detected in this experiment.

Keywords

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