• Bulletin of the Korean Chemical Society
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• v.27 no.4
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• pp.529-534
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• 2006

Chemical modification of the S. cerevisiae farnesyl protein transferase (FPT) with CMC, phenylglyoxal and DEPC resulted in enzyme inactivation, depending upon the reagent concentration. The peptide substrate GST-PEP-I, a GST-fused undecapeptide mimicking the C-terminus of $p21^{Ki-ras}$, protected the enzyme against inactivation by CMC which is specific to either aspartate or glutamate, while the other substrate farnesyl pyrophosphate (FPP) showed protection against phenylglyoxal which is the specific modifier of arginine residues, dependent on the substrate concentrations. Neither of the two substrates protected the enzyme against histidine inactivation by DEPC. It is suggested that there is at least one aspartate or glutamate residue at the peptide substrate binding site, and that at least one arginine residue is located at the binding site of FPP. There also seems to be at least one histidine residue which is critical for enzymic activity and is exposed toward the bulk solution, excluded from the substrate binding sites.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1577-1582
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• 1999

Carrot juice inoculated with $2\;{\times}\;10^8\;cfu/mL$ of Escherichia coli was treated with pulsed electric fields(PEF) for the purpose of a development of new cold pasteurization processes. Inactivation of E. coli in carrot juice increased with increase in intensity of the electric field strength and treatment time. The cells were suspended at concentration of ca. $2\;{\times}\;10^8$ cells per ml. A reduction of 4D was obtained at 40 kv/cm and 256 exponential decay pulses at room temperature. Critical electric field strength(Ec) and treatment time(tc) needed for inactivation of E. coli were 11.74 kV/cm and $3.6\;{\mu}s$ at room temperature, respectively. The combination of PEF and thermal treatment inactivated E. coli more effectively. The reductions of up to 5.5D were observed when the carrot juice was treated with PEF of 22.5 kV/cm and $205\;{\mu}s$ at $50^{\circ}C$. PFF treatment did not effect in color, pH, $^{\circ}Brix$, titratable acidity and ${\alpha}-,\;{\beta}-carotene$ contents of carrot juice.

• Food Science and Preservation
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• v.18 no.1
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• pp.98-102
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• 2011

We evaluated microbial inactivation in chicken cage litter, to ensure microbial safety, using aqueous chloride dioxide. Contamination by coliforms, Escherichia coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. was detected in fresh cage litter, and microbial populations increased if litters were repeatedly used. Aqueous $ClO_2$ treatment (500 ppm) significantly decreased the populations of coliforms, E. coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. in all litter samples tested. In particular, aqueous $ClO_2$ treatment on fresh litter reduced the initial populations of coliform, E. coli, Listeria spp., yeasts and molds, and total aerobic bacteria by 4.47, 1.29, 1.23, 3.24, and 5.2 log CFU/g, respectively. In addition, when litters used for 1 and 5 weeks were tested, treatment significantly reduced microbial populations. The results suggest that aqueous $ClO_2$ treatment is useful to reduce microbial hazards in chicken cage litter and to improve the microbial safety of slaughtered chickens.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1708-1716
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• 2013

Conventional molecular detection methods cannot distinguish between viable and dead Escherichia coli O157 cells. In this study, the loop-mediated isothermal amplification (LAMP) method combined with propidium monoazide (PMA) treatment was developed to selectively detect viable E. coli O157 cells. Four primers, including outer primers and inner primers, were specially designed for the recognition of six distinct sequences on the serogroups (O157) of the specific rfbE gene of the E. coli O157 genome. PMA selectively penetrated through the compromised cell membranes and intercalated into DNA. Amplification of DNA from dead cells was completely inhibited by $3.0{\mu}g/ml$ PMA, whereas the DNA derived from viable cells was amplified remarkably within 1 h by PMA-LAMP. Exhibiting high sensitivity and specificity, PMA-LAMP is a suitable method for evaluating the inactivation efficacy of slightly acidic electrolyzed water in broth. PMA-LAMP can selectively detect viable E. coli O157 cells. This study offers a novel molecular detection method to distinguish between viable and dead E. coli O157 cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.10
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• pp.1265-1269
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• 2017

This study was performed to evaluate the effects of combined treatment of clove bud essential oil (CBEO) and mild heat (MH) on inactivation of Escherichia coli O157:H7 inoculated onto red oak leaves. Combined treatment of 0.2% CBEO with MH at $50^{\circ}C$ exhibited the highest inhibitory effect against E. coli O157:H7 among treatments, resulting in 1.45 log reduction compared with water washing treatment. In addition, inhibitory effect of the combined treatment was maintained during storage of red oak leaves at $4^{\circ}C$ for 9 days, showing 1.67~2.25 log reductions compared with non-treated samples. Thus, these results indicate that combined treatment with CBEO/MH can be used to ensure the microbiological safety of fresh leaf vegetables such as red oak leaves during storage.

• Journal of Life Science
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• v.31 no.8
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• pp.755-760
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• 2021

Swine diarrhea is a livestock disease that causes huge economic losses to pig farms. In general, diarrhea occurs because of the proliferation of pathogenic Escherichia coli (E. coli). The toxins produced by the proliferated E. coli cause edema in pigs. Although the proliferation of these coliforms can be prevented by using a vaccine, the vaccines containing chemically produced dead bacteria are not very effective, making it difficult to control the proliferation of E. coli. Therefore, there is a need to develop new, more effective vaccines. In this study, we prepared killed F4+ and F18ab+ E. coli, which induce diarrhea and edema in pigs, using the extracts of immune-induced silkworms containing antimicrobial peptides and examined their availability as a killed-bacteria vaccine. First, the antimicrobial activity analysis of the prepared immune-induced silkworm extract was conducted using the radial diffusion assay. The results showed high activity against both F4+ and F18ab+ E. coli. The production efficiency of E. coli dead cells was determined using the colony-counting method. The concentration of the E. coli dead cells was the highest (50 mg/ml) when treated at 4℃. In addition, the analysis of the prepared dead cells using a transmission electron microscope confirmed that E. coli leaked out of the cytoplasm and the cell membrane remained intact. Therefore, F4+ and F18ab+ E. coli produced using immune-induced silkworms extract are considered to be highly available as bacterial ghost vaccines that can help prevent swine diarrhea and the resulting edema.

• Korean Journal of Food Science and Technology
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• v.29 no.4
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• pp.771-775
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• 1997

Treatment with electron beam irradiation was investigated for the elimination of Escherichia coli O157:H7 which has been linked to outbreaks of foodborne illness on undercooked and raw meat. Before treatment, the maximum populations were observed at 16 hr when E. coli O157:H7 was incubated in TSB at $37^{\circ}C$. Incubation at $4^{\circ}C$ did not influence survival and growth of the strain. The numbers of E. coli O157:H7 were present about $10^{7}\;CFU/mL$ in the log $(6\;hr\;at\;37^{\circ}C)$ and stationary phase $(16\;hr\;at\;37^{\circ}C)$ of cells, respectively. Freezing $(24\;hr\;at\;-18^{\circ})$ had a more marked lethal effect. The $D_{10}$ value at $-18^{\circ}C$ of E. coli O157:H7 contaminated in frozen beef was 0.45 kGy, and inactivation factor were $6.67{\sim}11.11$ at the radiation doses of $3{\sim}5\;kGy$. Therefore, electron beam irradiation was an effective method to eleminate of E. coli O157:H7.

• Bulletin of the Korean Chemical Society
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• v.24 no.12
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• pp.1799-1804
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• 2003

Acetolatate synthase(ALS) catalyzes the first common step in the biosynthesis of valine, leucine, isoleucine in plants and microorganisms. ALS is the target of several classes of herbicides, including the sulfonylureas, the imidazolinones, and the triazolopyrimidines. To elucidate the roles of arginine residues in tobacco ALS, chemical modification and site-directed mutagenesis were performed. Recombinant tobacco ALS was expressed in E. coli and purified to homogeneity. The ALS was inactivated by arginine specific reagents, phenylglyoxal and 2,3-butanedione. The rate of inactivation was a function of the concentration of modifier. The inactivation by butanedione was enhanced by borate, and the inactivation was reversible on removal of excess butanedione and borate. The substrate pyruvate and competitive inhibitors fluoropyruvate and phenylpyruvate protected the enzyme against inactivation by both modifiers. The mutation of well-conserved Arg198 of the ALS by Gln abolished the enzymatic activity as well as the binding affinity for cofactor FAD. However, the mutation of R198K did not affect significantly the binding of FAD to the enzyme. Taken together, the results imply that Arg198 is essential for the catalytic activity of the ALS and involved in the binding of FAD, and that the positive charge of the Arg is crucial for the interaction with negatively charged FAD.

• Particle and aerosol research
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• v.6 no.4
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• pp.185-192
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• 2010

This paper reports that the installation of a carbon fiber ionizer in front of an activated carbon fiber(ACF) filter enhanced the antibacterial efficiency. In addition, the effect of the ionizer on the filtration of bioaerosols is reported. Negative air ions from the ionizer were used as antibacterial agent. The test bacteria(Escherichia coli) were aerosolized using an atomizer and were deposited on the ACF filter media for 10 minutes. E. coli deposited on the filter were exposed to negative air ions for 0, 1, 5 and 10 minutes. Then they were separated from the ACF filter by shaking incubation with nutrient broth for 4 hours. The separated E. coli were spread on nutrient agar plates and incubated at $37^{\circ}C$ for 1~3 days. The antibacterial efficiency of E. coli was measured using a colony counting method. The antibacterial efficiencies of E. coli exposed to negative air ions for 1, 5 and 10 minutes were 14%, 48% and 71%, respectively. The filtration efficiency was evaluated by measuring the number concentration of bioaerosols at the upstream and downstream of the filter media. The increase of filtration efficiency by air ions was 14%, that is similar to the 17% filtration efficiency by none air ions. The ozone concentration was below the detection limit (under 0.01ppm) when the carbon fiber ionizers were on.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.7
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• pp.1009-1013
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• 2012

We investigated the antibacterial effects of Opuntia ficus indica extracts on foodborne pathogens, Escherichia coli O157:H7 and Listeria monocytogenes, on the medium of sliced apples. Pathogens were inoculated on sliced apples and immersed for 10 min in Opuntia ficus indica extracts. Each sample was packaged and stored at $4^{\circ}C$ and $21^{\circ}C$ for 8 days. The populations of E. coli O157:H7 and L. monocytogenes significantly decreased with increasing extract concentration (p<0.05). In particular, L. monocytogenes was reduced to non-detectable levels after 2 days in 50 mg/mL treatment at $4^{\circ}C$ and $21^{\circ}C$ Opuntia ficus indica extracts therefore have antibacterial effects on the two foodborne pathogens. Sensory evaluation results indicated that treated apples had better sensory characteristics than did the control. Therefore, the results suggest that Opuntia ficus indica extracts could be useful as a natural food preservative to improve microbial safety.