• Title/Summary/Keyword: in-structure amplification

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Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

  • Chauhan, Puneet Singh;Shagol, Charlotte C.;Yim, Woo-Jong;Tipayno, Sherlyn C.;Kim, Chang-Gi;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.1
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    • pp.127-145
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    • 2011
  • Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.

Hydraulic experiments on wave amplification at concave corner for rubble mound structures (경사식구조물 오목부 구간의 파랑증폭 실험)

  • Kim, Young-Taek;Ahn, Chang-Hyun;Lee, Jong-In
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.6
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    • pp.3074-3080
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    • 2013
  • Amplification of wave height at concave corner was investigated by three dimensional hydraulic tests. A typical rubble mound structure was tested with two-layer Tetrapod and the slope of 1:1.5. The irregular waves with Bretschneider-Mitsuyasu spectrum were applied to the tests. The center angles of concave corner were 120 degree, 140 degree and 160 degree. According to the test results, the maximum wave height amplification ratio at concave corner was about 1.5 times of incident wave height among the all test conditions, and the W-shaped wave height distribution was shown.

Structure optimization of a L-band erbium-doped fiber amplifier for 64 optical signal channels of 50 GHz channel spacing (50 GHz 채널 간격의 64 채널 광신호 전송을 위한 L-band EDFA의 구조 최적화)

  • Choi, Bo-Hun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.11
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    • pp.1666-1671
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    • 2022
  • The structure of a high-power gain-flattened long wavelength band (L-band) optical amplifier was optimized, which was implemented for 64-channel wavelength division multiplexed optical signals with a channel spacing of 50 GHz. The output characteristics of this L-band amplifier were measured and analyzed. The amplifier of the optimized two-stage amplification configuration had a flattened gain of 20 dB within 1 dB deviation between 1570 and 1600 nm for -2 dBm input power condition. The noise figure under this condition was minimized to within 6 dB in the amplification bandwidth. The gain flattening was realized by considering only the characteristics of gain medium in the amplifier without using additional optical or electrical devices. The proposed amplifier consisted of two stages of amplification stages, each of which was based on the erbium-doped fiber amplifier (EDFA) structure. The erbium-doped fiber length and pumping structures in each stage of the amplifier were optimized through experiments.

Design, Fabrication and Test of Piezoelectric Actuator Using U-Shape PZT Strips and Lever Structure for Lateral Stroke Amplification (수평방향 변위증폭을 위해 U-형상의 PZT 스트립과 지렛대 구조를 이용한 압전구동형 액추에이터의 설계, 제작 및 실험)

  • 이준형;이택민;최두선;황경현;서영호
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.28 no.12
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    • pp.1937-1941
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    • 2004
  • We present lateral actuated piezoelectric actuator using U-shaped PZT strip and lever structure for the RF switch application. In the previous study of RF switch, they used horizontal contact switch fabricated by thin film metals. However, thin film metals could not generate large contact force due to low stiffness. In this work, we suggest lateral contact switch which makes large contact force by increasing stiffness. In addition, we use PZT actuator for the high force actuation. Generally actuator using thin film PZT moves to the vertical direction due to the neutral axis shift. Therefore we need lateral motion generation mechanism based on the thin film PZT actuator. In order to increase lateral motion of thin film PZT actuator, we use U-shaped PZT actuator using residual stress control. Also, thin film PZT actuator can generate very small lateral motion of 120${\times}$10$^{-6}$ ${\mu}{\textrm}{m}$/V for d$_{31}$ mode, thus we suggest lever structure to increase stroke amplification. From the experimental study, fabricated PZT actuator shows maximum lateral displacement of 1 ${\mu}{\textrm}{m}$, and break down voltage of the thin film PZT actuator is above 16V.

Seismic Reliability Analysis of Offshore Wind Turbine Jacket Structure Using Stress Limit State (응력한계상태를 이용한 해상풍력발전기 재킷구조물의 지진신뢰성해석)

  • Lee, Gee-Nam;Kim, Dong-Hyawn
    • Journal of Ocean Engineering and Technology
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    • v.30 no.4
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    • pp.260-267
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    • 2016
  • Considering the effect of dynamic response amplification, a reliability analysis of an offshore wind turbine support structure under an earthquake is presented. A reliability analysis based on the dynamic response requires a large amount of time when using not only a level 3 approach but also level 2 such as a first order reliability method (FORM). Moreover, if a limit state is defined by using the maximum stress at a structural joint where stress concentration occurs, a three-dimensional element should be used in the finite element analysis. This makes the computational load much heavier. To deal with this kind of problem, two techniques are suggested in this paper. One is the application of a quasi-static structural analysis that takes the dynamic amplification effect into account. The other is the use of a stress concentration factor to estimate the maximum local stress. The proposed reliability analysis is performed using a level 2 FORM and verified using a level 3 simulation approach.

Slope topography effect on the seismic response of mid-rise buildings considering topography-soil-structure interaction

  • Shabani, Mohammad J.;Shamsi, Mohammad;Ghanbari, Ali
    • Earthquakes and Structures
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    • v.20 no.2
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    • pp.187-200
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    • 2021
  • The main factor for the amplification of ground motions near the crest or the toe of a slope is the reflection of the incident waves. The effects of the slope topography on the surrounding lands over the crest or at the toe can amplify the seismic responses of buildings. This study investigates the seismic performance of the slope topography and three mid-rise buildings (five, ten, and fifteen-storey) located near the crest and toe of the slope by 3D numerical analysis. The nonlinear model was used to represent the real behavior of building and ground elements. The average results of seven records were used in the investigations. Based on the analysis, the amplification factor of acceleration near the crest and toe of the slope was the most effective at distances of 2.5 and 1.3 times the slope height, respectively. Accordingly, the seismic performance of buildings was studied at a distance equal to the height of the slope from the crest and toe. The seismic response results of buildings showed that the slope topography to have little impact on up to five-storey buildings located near the crest. Taking into account a topography-soil-structure interaction system increases the storey displacement and base shear in the building. Accordingly, in topography-soil-structure interaction analyses, the maximum lateral displacement was increased by 71% and 29% in ten and fifteen-storey buildings, respectively, compare to the soil-structure interaction system. Further, the base shear force was increased by 109% and 78% in these buildings relative to soil-structure interaction analyses.

Evaluation of moment amplification factors for RCMRFs designed based on Iranian national building code

  • Habibi, Alireza;Izadpanah, Mehdi;Rohani, Sina
    • Advances in concrete construction
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    • v.9 no.1
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    • pp.23-31
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    • 2020
  • Geometric nonlinearity can significantly affect load-carrying capacity of slender columns. Dependence of structural stability on columns necessitates the consideration of second-order effects in the design process of columns, appropriately. On the whole, the design codes present a simplified procedure for second order analysis of slender columns. In this approximate method, the end moments of columns resulted from linear analysis (first-order) are multiplied by the recommended moment amplification factors of codes to achieve magnified moments of the second-order analysis. In the other approach, the equilibrium equations are directly solved for the deformed configuration of structure, so the resulting moments and deflections contain the influence of slenderness and increase more rapidly than do loads. The aim of this study is to evaluate the accuracy of moment amplification factors of Iranian national building code whose provisions are similar to the ACI requirement. Herein, finite element method is used to achieve magnified end moments of reinforced concrete moment resisting frames, and the outcomes are compared with the moments acquired based on the proposed approximate method by Iranian national building code. The results show that the approximate method of Iranian code for calculating magnified moments has significant errors for both unbraced and braced columns.

Response of a frame structure on a canyon site to spatially varying ground motions

  • Bi, Kaiming;Hao, Hong;Ren, Weixin
    • Structural Engineering and Mechanics
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    • v.36 no.1
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    • pp.111-127
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    • 2010
  • This paper studies the effects of spatially varying ground motions on the responses of a bridge frame located on a canyon site. Compared to the spatial ground motions on a uniform flat site, which is the usual assumptions in the analysis of spatial ground motion variation effects on structures, the spatial ground motions at different locations on surface of a canyon site have different intensities owing to local site amplifications, besides the loss of coherency and phase difference. In the proposed approach, the spatial ground motions are modelled in two steps. Firstly, the base rock motions are assumed to have the same intensity and are modelled with a filtered Tajimi-Kanai power spectral density function and an empirical spatial ground motion coherency loss function. Then, power spectral density function of ground motion on surface of the canyon site is derived by considering the site amplification effect based on the one dimensional seismic wave propagation theory. Dynamic, quasi-static and total responses of the model structure to various cases of spatially varying ground motions are estimated. For comparison, responses to uniform ground motion, to spatial ground motions without considering local site effects, to spatial ground motions without considering coherency loss or phase shift are also calculated. Discussions on the ground motion spatial variation and local soil site amplification effects on structural responses are made. In particular, the effects of neglecting the site amplifications in the analysis as adopted in most studies of spatial ground motion effect on structural responses are highlighted.

Shear forces amplification due to torsion, explicit reliance on structural topology. Theoretical and numerical proofs using the Ratio of Torsion (ROT) concept

  • Bakas, Nikolaos
    • Structural Engineering and Mechanics
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    • v.61 no.1
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    • pp.15-29
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    • 2017
  • The recently introduced index Ratio Of Torsion (ROT) quantifies the base shear amplification due to torsional effects on shear cantilever types of building structures. In this work, a theoretical proof based on the theory of elasticity is provided, depicting that the ratio of torsion (ROT) is independent of the forces acting on the structure, although its definition stems from the shear forces. This is a particular attribute of other design and evaluation criteria against torsion such as center of rigidity and center of strength. In the case of ROT, this evidence could be considered as inconsistent, as ROT is a function solely of the forces acting on structural members, nevertheless it is proven to be independent of them. As ROT is the amplification of the shear forces due to in-plan irregularities, this work depicts that this increase of internal shear forces rely only on the structural topology. Moreover, a numerical verification of this theoretical finding was accomplished, using linear statistics interpretation and nonlinear neural networks simulation for an adequate database of structures.

A Study on the Dynamic Amplification Characteristics of the Domestic Seismic Observation Sites using Shear- and Coda-Wave (S파 및 Coda파를 이용한 국내 관측소지반의 동적 증폭특성에 관한 연구)

  • Kim, Jun-Kyoung
    • Tunnel and Underground Space
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    • v.19 no.5
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    • pp.432-439
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    • 2009
  • For more reliable estimation of seismic source, attenuation properties and dynamic ground property, site amplification function should be considered. Among various estimation methods, this study used the Nakamura's method (1989) for estimating site amplification characteristics. This method was originally applied to the surface waves of background noise and therefore there are some limitations in applying to general wave energy. However, recently this method has been extended and applied to the S wave energy successfully. This study applied the method to S wave and Coda wave energy, which is equivalent to the backscattered S wave energy. We used more than 60 observed ground motions from 5 earthquakes which were occurred recently, with magnitude range from 3.6 to 5.1. Each station showed characteristic site amplification property in low-, high- and resonance frequencies. Some of the stations showed as high as 4 times of site amplification in the range of specific frequencies, which may imply abnormal small scale geologic strata below the station or development of various trapped modes in the basin structure. Moreover, removal of site amplification can give us more reliable seismic source and attenuation parameters, addition to the seismic hazard estimation.