• Title/Summary/Keyword: in vitro protein digestibility

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Evaluation the Feed Value of Whole Crop Rice Silage and Comparison of Rumen Fermentation according to Its Ratio (신규 조사료원 사료용 벼 사일리지의 사료가치 평가 및 급여 비율에 따른 반추위 발효성상 비교)

  • Park, Seol Hwa;Baek, Youl Chang;Lee, Seul;Kim, Byeong Hyeon;Ryu, Chae Hwa
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.4
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    • pp.236-243
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    • 2020
  • This study was to evaluate the feed value of whole crop rice silage (WCRS) and to investigate a suitable ratio of the WCRS and concentrate by an analysis of rumen fermentation. A total of 6 treatments were used according to WCRS: concentrate ratio on in vitro rumen fermentation: T1 (100:0), T2 (60:40), T3 (40:60), T4 (20:80), T5 (10:90), and T6 (0:100). The ruminal pH, total gas emission, ammonia nitrogen, and volatile fatty acid (VFA) were determined as fermentation parameters. Total nutrients digestibility trial was conducted by 4 treatments according to WCRS: concentrate ratio at 40:60 (W40), 20:80 (W20), and 10:90 (W10), respectively. Feed value was analyzed according to AOAC (2019) and nutrient digestibility was calculated based on NRC (2001). The levels of crude protein (CP), crude fat, and neutral detergent fiber of the WCRS were 12.29%, 1.67%, and 59.79%, respectively. It was found to be 51.49% as a result of predicting the total digestible nutrient of WCRS using the NRC (2001) model. In vitro rumen fermentation, T4, T5, and T6 treatments showed a greater gas emission and total VFA concentration compared with other treatments (p<0.05). Acetate and acetate to propionate ratio of T4, T5, and T6 were significantly higher than other treatments (p<0.05). There was a significant difference in the level of propionate and butyrate according to the WCRS: concentrate ratio (p<0.05). The digestibility of dry matter and CP was significantly lower in W40 than in other treatments (p<0.05); however, there was no difference in W20 and W10. In conclusion, the 20:80 (WCRS: concentrate) is beneficial for stabilizing the rumen that does not inhibit rumen fermentation and nutrient digestion. This ratio might have a positive effect on the economics of farms as a valuable feed.

Lactobacillus plantarum Improves the Nutritional Quality of Italian Ryegrass with Alfalfa Mediated Silage

  • Ilavenil, Soundarrajan;Arasu, Mariadhas Valan;Vijayakumar, Mayakrishnan;Jung, Min-Woong;Park, Hyung Soo;Lim, Young Cheol;Choi, Ki Choon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.3
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    • pp.174-178
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    • 2014
  • The present study was planned to analyze the nutritional quality, microbial counts and fermentative acids in Italian ryegrass (IRG) 80% and alfalfa 20% (IRG-HV) mediated silage inoculated with lactic acid bacteria (LAB) as a probiotic strain for 3 months. Crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), total digestible nutrient (TDN) and In-vitro dry matter digestibility (IVDMD), lactic acid bacteria (LAB), yeast and fungi counts and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. The result shows that the nutritional quality and metabolite profiles of silage were significantly improved with LAB. For microbial counts, LAB showed dominant followed by yeast as compared with control silage. The pH of the silage also reduced significantly when silage inoculated with LAB. The result confirmed that silage preparation using different crops with L. plantarum inoculation is most beneficial for the farmers.

Determining the Harest Time for Maximum Yield and Qualty of Ochardgrass Swards (Orchardgress 단파초지의 최고생산과 질적향상을 위한 취확적기의 결정)

  • Chang, Nam-Kee;Lee, Sung-Kyu;Kim, Sung-Ha
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.3 no.2
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    • pp.86-91
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    • 1983
  • The harvest schedule for maximum total yield and quality of orchardgrass sward was studied on the basis of theoretical analysis due to Chang's total yield equation. Harvesting at the 10% bloom stage of morphological development of orchardgrass swards was selected as a criterion which provided highest dry matter (DM) yield in conjunction with high and consistent levels of in vitro dry matter digestibility (IVD) and crude protein contents (CP) from harvest to harvest. However, since orchardrass after the 1st cutting do not bloom, the harvest time for the maximum yield and quality of orchardgrass swards is replaced by the three should time of the ceiling leaf area index (LAI).

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Changes in fermentation pattern and quality of Italian ryegrass (Lolium multiflorum Lam.) silage by wilting and inoculant treatments

  • Liu, Chang;Zhao, Guo Qiang;Wei, Sheng Nan;Kim, Hak Jin;Li, Yan Fen;Kim, Jong Geun
    • Animal Bioscience
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    • v.34 no.1
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    • pp.48-55
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    • 2021
  • Objective: This study was conducted to investigate the effects of wilting and microbial inoculant treatment on the fermentation pattern and quality of Italian ryegrass silage. Methods: Italian ryegrass was harvested at heading stage and ensiled into vinyl bags (20 cm×30 cm) for 60d. Italian ryegrass was ensiled with 4 treatments (NWNA, no-wilting noadditive; NWA, no-wilting with additive; WNA, wilting no-additive; WA, wilting with additive) in 3 replications, wilting time was 5 hours and additives were treated with 106 cfu/g of Lactobacillus plantarum. The silages samples were collected at 1, 2, 3, 5, 10, 20, 30, 45, and 60 days after ensiling and analyzed for the ensiling quality and characteristics of fermentation patterns. Results: Wilting treatment resulted in lower crude protein and in vitro dry matter digestibility and there were no significant differences in acid detergent fiber (ADF), total digestible nutrient (TDN), water-soluble carbohydrate (WSC), ammonia content, and pH (p>0.05). However, wilting treatment resulted in higher ADF and neutral detergent fiber content of Italian ryegrass silage (p<0.05), and the WNA treatment showed the lowest TDN and in vitro dry matter digestibility. The pH of the silage was higher in the wilting group (WNA and WA) and lower in the additive treatment group. Meanwhile, the decrease in pH occurred sharply between the 3-5th day of storage. The ammonia nitrogen content was significantly lower in the additive treatment (p<0.05), and wilting had no effect. As fermentation progressed, the lactic and acetic acid contents were increased and showed the highest content at 30 days of storage. Conclusion: The wilting treatment did not significantly improve the silage fermentation, but the inoculant treatment improved the fermentation patterns and quality of the silage. So, inoculation before ensiling is recommended when preparing high quality of Italian ryegrass silage, and when wilting, it is recommended to combine inoculation for making high quality silage.

On-site Output Survey and Feed Value Evaluation on Agro- industrial By-products (농산업부산물들에 대한 배출 현장 조사 및 사료적 가치 평가)

  • Kwak, W. S.;Yoon, J. S.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.251-264
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    • 2003
  • This study was conducted to make on-site survey on the output pattern and utilization situation of 19 by-products selected, to evaluate their nutritional characteristics, to find out a reliable index with which digestion of by-products can be predicted on the basis of chemical compositions analyzed and to diagnose the risk of using book values in the absence of the actual values analyzed for diet formulation. Production and utilization situations of by-products were quite various. Nutritionally, fruit processing by-products such as apple pomace (AP), pear pomace (PP), grape pomace (GP), and persimmon peel (PSP), and bakery by-products (BB) were classified as energy feeds. Soybean curd meal (SCM), animal by- products such as blood (BD), feather meal (FM) and poultry by-products (PB), and activated milk processing sludge (AMS) were classified as protein feeds. Soy hulls (SH), spent mushroom compost (SMC), barley malt hulls (BMH), waste paper (WP) and broiler litter (BL) were classified as roughage. Rumen contents (RC) and restaurant food waste (FW) were nutritionally analogous to complete diets for cattle and swine, respectively. Compared to soybean meal (SBM), BD and FM contained high (P<0.05) levels of amino acids and barley malt sprouts (BMS), AMS and FW contained low (P<0.05) levels of amino acids. Enzymatic (pepsin) digestibilities of proteinaceous feeds ranged between 99 and 66%. In vitro DM digestibility was high (P<0.05) in the order of FW, BB, AP, SH, PP, PSP, BMH, BMS, SCM, GP, RC, PB, BL, WP, SMC, AMS, FM and BD. In vitro DM digestibility had the highest correlation (r=0.68) with nonfibrous carbohydrate among chemical components. Differences between analyzed values of chemical components and book values were considerable. Caution is required in using book values when large amount of by-products are used in diets.

Evaluation of Characteristics and Forage Production for Bermudagrass (Cynodon dactylon) and Bahiagrass (Paspalum notatum) in Jeju (제주지역에서 버뮤다그라스 및 바히아그라스의 생육특성 및 사초생산성 평가)

  • Park, Hyung-Soo;Park, Nam-Gun;Kim, Jong-Gun;Choi, Ki-Choon;Lim, Young-Chul;Choi, Gi-Jun;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.2
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    • pp.131-138
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    • 2012
  • This study was conducted to evaluate the characteristics and forage production of bermudagrass (Cynodon dactylon) and bahiagrass (Paspalum notatum) in Jeju. Bermudagrass cultivars evaluated were Common and Ecotype. Bahiagrass cultivars evaluated were Tifton 9 and Argentine. Two warm season grasses were established at the Subtropical Animal Experiment Station in spring 2007. Emergence of bremudagrass and bahiagrass was observed approximately 16 days and 28 days after seeding, respectively. The heading dates of bermudagrass and bahiagrass were on 26 May and in mid-July, respectively. Bermudagrass cultivars had higher dry matter (DM) than bahiagrass at first harvest. Dry matter yield of bahiagrass was higher than that of bermudagrass (p<0.05). Peak forage DM production of bermudagrass and bahiagrass cultivars was in June and July, respectively. The content of crude protein (CP) and total digestibility nutrient (TDN) of bermudagrass cultivars were higher than those of bahiagrass during the first harvest. Acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across the four cultivars. In Jeju, bermudagrass and bahiagrass provide a useful option for supplemental summer forage in most livestock forage systems.

Effects of different ratios and storage periods of liquid brewer's yeast mixed with cassava pulp on chemical composition, fermentation quality and in vitro ruminal fermentation

  • Kamphayae, Sukanya;Kumagai, Hajime;Angthong, Wanna;Narmseelee, Ramphrai;Bureenok, Smerjai
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.4
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    • pp.470-478
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    • 2017
  • Objective: This study aims to evaluate the chemical composition, fermentation quality and in vitro ruminal fermentation of various ratios and storage periods of liquid brewer's yeast (LBY) mixed with cassava pulp (CVP). Methods: Four mixtures of fresh LBY and CVP were made (LBY0, LBY10, LBY20, and LBY30 for LBY:CVP at 0:100, 10:90, 20:80, and 30:70, respectively) on a fresh matter basis, in 500 g in plastic bags and stored at 30 to $32^{\circ}C$. After storage, the bags were opened weekly from weeks 0 to 4. Fermentation quality and in vitro gas production (IVGP) were determined, as well as the dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber, acid detergent fiber and acid detergent lignin contents. Results: The contents of CP and EE increased, whereas all other components decreased, in proportion to LBY inclusion (p<0.01). The DM and OM contents gradually decreased in weeks 3 and 4 (p<0.05), while EE contents were lowest in week 0. The pH, ammonia nitrogen per total nitrogen ($NH_3-N/TN$) and V-score in each mixture and storage period demonstrated superior fermentation quality ($pH{\leq}4.2$, $NH_3-N/TN{\leq}12.5%$, and V-score>90%). The pH increased and $NH_3-N/TN$ decreased, with proportionate increases of LBY, whereas the pH decreased and $NH_3-N/TN$ increased, as the storage periods were extended (p<0.01). Although IVGP decreased in proportion to the amount of LBY inclusion (p<0.01), in vitro organic matter digestibility (IVOMD) was unaffected by the mixture ratios. The highest IVGP and IVOMD were observed in week 0 (p<0.01). Conclusion: The inclusion of LBY (as high as 30%) into CVP improves the chemical composition of the mixture, thereby increasing the CP content, while decreasing IVGP, without decreasing fermentation quality and IVOMD. In addition, a preservation period of up to four weeks can guarantee superior fermentation quality in all types of mixtures. Therefore, we recommend limiting the use of CVP as a feed ingredient, given its low nutritional value and improving feed quality with the inclusion of LBY.

Study on Supplementing Effects or Feeding Systems of Molasses and Urea on Methane and Microbial Nitrogen Production in the Rumen and Growth Performances of Bulls Fed a Straw Diet

  • Huque, K.S.;Chowdhury, S.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.1
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    • pp.35-46
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    • 1997
  • An experiment with growing bulls were conducted to determine the effect of supplementation of a straw (S) with 15% molasses and 3% urea as an intimate mix (UMS) on its dry matter (DM) intake and digestibility (DMD) and reduction of methane ($CH_4$) production from fermentation in vitro of the straw. In the next experiment, the feeding of the UMS was compared with that of the feeding of molasses and urea in meals (DS) or in lick blocks (DSUMB) as supplements to straw. The UMS feeding increased daily intake of straw DM ($89.5 g{\cdot}kgW^{-0.75}$, p < 0.01) and digestible crude protein (DCP 333 g, p < 0.001) and nitrogen (N) balances ($508mg{\cdot}kgW^{-0.75}{\cdot}d^{-1}$, p < 0.01) of the bulls than the feeding of 'S' ($65g{\cdot}kgW^{-0.75}$, 55 g and $8.0mg{\cdot}kgW^{-0.75}{\cdot}d^{-1}$, respectively). It also increased the digestibility of DM ($594g{\cdot}kg^{-1}$, p < 0.05), organic matter (OM, $641g{\cdot}kg^{-1}$, p < 0.05), CP ($619g{\cdot}kg^{-1}$, p < 0.001) and acid detergent fibre (ADF, 773, p < 0.05). The $CH_4$ emitted per g of DOM apparently fermented in the rumen (DOMR) was 91.0 ml in the 'S' and reduced (p < 0.05) to 61.6 ml in the UMS. The feeding of the UMS when compared with that of the DS or DSUMB also gave a higher straw intake (1.77% of live weight, LW, p <0.01) and ADF digestibility ($516g{\cdot}kg^{-1}$, p < 0.05) than the other diets (1.52% or 1.55% LW and 472 or $490g{\cdot}kg^{-1}$, respectively) in association with the increased microbial N yield in the rumen (14.1, 5.62 or $17.0g{\cdot}kg^{-1}$ DOMR, respectively, p < 0.05), daily LW gains (233, 125 or 93 g, respectively, p < 0.05) and feed conversion ratios of the diets (26.0, 56.1, or 57.6 g feed/g LW gain, p > 0.05, respectively). It can be concluded that molasses and urea feeding as an intimate mix with straw (UMS) increased its digestion and intake in association with a reduced methane emissions in the rumen. When compared with that of their feeding in meals or in lick blocks as supplements to straw the UMS gave the highest straw in take and digestion and live weight gains of growing bulls concurring the finding that the UMS system may be the best way of molasses and urea feeding to ruminants fed straws.

Comparison of Growth Characteristics, Forage Production and Feed Values of Bermudagrass, Teffgrass and Kleingrass as Annual Forage Crop in Summer (여름철 버뮤다그라스, 클라인그라스 및 테프그라스의 생육특성, 생산성 및 사료가치 비교)

  • Park, Hyung Soo;Choi, Ki Choon;Kim, Ji Hea;Lee, Sang-Hoon;Jung, Jong Seong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.1
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    • pp.36-42
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    • 2015
  • This study was conducted from 2013 to 2014 to explore the feasibility of the cultivation of warm-season grass as an annual Korean forage crop, while concurrently evaluating the characteristics and forage production of warm-season grass in Korea. The experimental design was a randomized block design (RBD) with three replications. Five bermudagrass [Cynodon dactylon (L.) Pers.] cultivars, two teffgrass (Paspalum notatum Flugge) cultivars, and a Kleingrass [Panicum coloratum L.] cultivar were compared for forage production and quality in the middle region of Korea. After seeding, the numbers of days until seedling emergence for bermudagrass and kleingrass were observed at approximately day 11 and day 12, respectively. The heading dates of teffgrass and kleingrass were on July 12 and July 26, respectively. The dry matter (DM) yield of bermudagrass Tifton 85 was usually greater than the other entries. The crude protein content (CPC) and total digestibility nutrient (TDN) for the teffgrass cultivars were usually greater than the other entries at all study sites. The acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across all cultivars.

A COMPARATIVE STUDY OF THE PROTECTION OF DHAINCHA (Sesbania aculeata) SEED MEAL AND FISH MEAL FROM RUMEN DEGRADATION USING NYLON BAG TECHNIQUE

  • Hussain, M.;Chowdhury, B.;Siddiqua, A.;Routh, C.K.;Saadullah, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.2
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    • pp.197-203
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    • 1993
  • The protection of dry matter and nitrogen from rumen degradation of dhaincha seed (Sesbania aculeate) meal was studied using nylon bag technique. The dhaincha seed meal was subjected to various heat treatments that included oven drying, autoclaving and boiling. Similar experiment was conducted with fish meal as reference for comparison. The oven-dried dhaincha meal was found to retain more dry matter and nitrogen than was found boiled or autoclaved meal. While autoclaving appeared to improve nitrogen and dry matter retention to some extent, boiling seemed to cause more loss of dry matter from nylon bag. Heat treatment caused high retention of nitrogen by fish meal. The calculated effective protein degradation was 80.4% and 83.2% for the oven dried fish meal and dhaincha seed meal whereas same values were 74.2% and 86.7% for autoclaved fish and dhaincha seed meal respectively at the outflow rate of 4.4% per hour. The in vitro study revealed higher digestibility for heat treated samples by pepsin. The dry matter, nitrogen and ash content of dhaincha seed meal were 85.93%, 5.93% and 7.31% respectively.